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ISOLASI DAN KARAKTERISASI LIPASE DARI KECAMBAH BIJI ALPUKAT (Persea americana Mill) Syabani, Nurillah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Isolation and determination of optimum lipase conditions from Avocado seeds germination (Persea americana Mill) have been done. The purpose of this study is to determine the optimum working conditions and lipase specific activity of avocado seeds germination. Lipase was isolated using homogenization and centrifugation techniques. The crude extract of the enzyme containing the lipase produced from avocado seeds germination was used for the determination of total protein concentration and tested its activity on the pH conditions, temperature, and optimum substrate concentration with different with various variations. Determination of total concentration was done by Bradford method, while the determination of optimum working conditions and lipase specific activity was performed by titrimetric method. The results showed that total protein concentration in avocado seeds germination was 458 μg / mL. The optimum working conditions on avocado seed sprouts are the degree of acidity (pH) 6, temperature 35 oC, and 1.5% v/v substrate concentration. The lipase specific activity of avocado seeds germination is 144,1 U / mg.
SCREENING BAKTERI PENGHASIL AMILASE DARI SEDIMEN SUMBER AIR PANAS DONDANG MUARA JAWA Fahrul Fhozi, M Nur Yasin; Astuti, Winni; Gunawan, Rahmat
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The screening of amylase enzyme producing thermophilic bacteria from sediment of hot spring in Muara Jawa has been done. Screening has done by using selective media of starch, will show the formation of clear zones around the colonies by dripping the iodine solution. From alot colonies selected 1 colony with  widest clear zone. Single colony obtained was identified as Bacillus sp. Amylase produced was tested for amylase activity by DNS method and obtained the amylase activity at pH 6 and temperature at 55°C is 2,8460 U/mL.
STUDI DOCKING MOLEKULAR SENYAWA KUERSETIN, KALKON DAN TURUNANNYA SEBAGAI INHIBITOR SEL KANKER PAYUDARA MC-7 (MICHIGAN CANCER FOUNDATION-7) Lelita, Resy; Gunawan, Rahmat; Astuti, Winni
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Molecular Docking studies Quercetin, Chalcone and its derivate inhibitor to breast cancer cells MCF-7 (Michigan Cancer Foundation-7) have been done. The results showed that the docking energies after the process of binding on mode 1 of four compounds Quersetin -7.4 Kcal/mol, 2-(3,4-dihidroksifenil)-5,7-dihidroksi-3-metoksi-3,4-dihidro-2H-1-benzopyran-4-on -6.8 Kcal/mol, Chalcone -6.7 Kcal/mol and 3-(2,4-dihidroksifenil)-1-fenilpropan-1-on -6.6 Kcal/mol. Quercetin formed hydrogen bondings with amino acid  of Aspartic Acid 61, Arginine 65, Arginine 68, Fenilalanin 71 and Serin 75. , 2-(3,4-dihidroksifenil)-5,7-dihidroksi-3-metoksi-3,4-dihidro-2H-1-benzopyran-4-on formed hydrogen bondings with amino acid of Alanin 59, Asparagine 102 dan Arginine 105. Chalcone formed hydrogen bondings with amino acid of Glitamic Acid 94 and 3-(2,4-dihidroksifenil)-1-fenilpropan-1-on formed hydrogen bondings with amino acid of Tyrosine 67. Quercetin is as potential than anticancer drug than chalcone, -(3,4-dihidroksifenil)-5,7-dihidroksi-3-metoksi-3,4-dihidro-2H-1-benzopyran-4-on and 3-(2,4-dihidroksifenil)-1-fenilpropan-1-on.   Keywords: Breast Cancer, Molecular Docking, Quercetin, Kalkon, Protein Breast Cancer Cells MCF-7 (Michigan Cancer Foundation-7).
ISOLASI AMILASE DARI KECAMBAH BIJI NANGKA (Artocarpus heterophyllus Lam) Fahmi, Ismi; Astuti, Winni; Sitorus, Saibun
JURNAL ATOMIK Vol 2 No 1 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

This research aims to isolate the crude extract amylase of sprouted seeds jackfruit (Artocarpus heterophyllus Lam) and determine the specific activity. Determination of total protein concentration and amylase activity of enzyme crude extract on seed germination jackfruit (Artocarpus heterophyllus Lam). Determination of total protein concentration using the method of Bradford, whereas the determination of amylase activity was performed using dinitrosalisilat acid method (DNS). The results showed a total protein concentration of the enzyme crude extract of sprout seeds jackfruit (Artocarpus heterophyllus Lam) is 319,47 ug / mL, amylase activity of 13,96 U / mL and specific activity is 1748,05 U / mg. 
PENENTUAN KADAR ASAM AMINO ESENSIAL (FENILALANIN, TREONIN, TIROSIN DAN VALIN) PADA TELUR PENYU DAN TELUR AYAM RAS Lebang, Agnes; Sitorus, Saibun; astuti, winni
JURNAL KIMIA MULAWARMAN Vol 15 No 2 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Determination of amino acid essential’s content (phenylalanin, threonin, tyrosin and valine) on turtle eggs and chicken eggs
AKTIVITAS AMILASE, PROTEASE dan LIPASE DARI MADU LEBAH Trigona sp, Apis mellifera dan Apis dorsata Putra, Hendric Surya; Astuti, Winni; Kartika, Rudi
JURNAL KIMIA MULAWARMAN Vol 16 No 1 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.
SCREENING BACTERIAL OF ENDOPHYTIC PRODUCING AMYLASE, LIPASE AND PROTEASE FROM LEAVES OF Macaranga hullettii King ex Hook.f. Pricilia, Sherly; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The objective of this study was to screening test of endophytic bacteria producing amylase, lipase and protease from leaves Macaranga hullettii King ex Hook.f. Endophytic bacteria isolation was performed on nutrient agar (NA) and 21 single colonies of endophytic bacteria were obtained. Endophytic bacteria that all of the endophytic bacteria positive producing enzym, 8 of the endophytic bacteria positive amylase, 7 of the endophytic bacteria positive lipase and 4 of the endophytic bacteria positive protease.  
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE DARI TALAS BOGOR (Colocasia esculenta (L.) shoot) Estrada, Rezky; Kartika, Rudi; Astuti, Winni
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Isolation and determination of optimum amylase work condition has been conducted from Bogor taro (Colocasia esculenta (L.) shoot). The aim of this research is to understand the optimum work condition and specific activity of amylase from Bogor taro. Amylase was isolated using homogenization techniques and sentrifugation. The raw extract of enzyme that contain amylase produced by Bogor taro then used to determine the total protein concentration and tested its activity in pH condition, temperature and maximum substrate concentration in various ways. The determination of total protein concentration was done with Bradford method, while the optimum work condition and specifik activity of amylase was done with dinitrosalycylic acid (DNS) method. The result of this reseach show the total protein concentration in Bogor taro is 7,88 μg/mL. Optimum work condition in Bogor taro is on acidity level (pH) 6, 600C temperature and 6 mg/mL substrate concentration. Specific activity from Bogor taro is 9573,68 U/mL.   Keywords:  amylase, taro tuber Bogor (Colocasia esculenta  (L.) shoot), specific activity of amylase.  
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE PADA UMBUT KELAPA (Coccos nucifera L.) Nuryanti, Lila Karina; Astuti, Winni; Kartika, Rudi
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Amylase is an enzyme that can catalyze stach hydrlysis into its simplier molecule as glucose. Coconut’s buds (Coccos nucifera L.) have high enough of carbohydrate content which amylase could have existed. The research are to determine protein concentration, optimum catalyst function and specified activity of amylase in coconut’s buds (Coccos nucifera L.). Coconut’s buds (Coccos nucifera L.) are isolated by extraction method using phospate buffer solution 0,1 M pH 7. The determination of protein concentration are used Bradford method. The determination of optimum catalyst function are used dinitrosalicylate acid (DNS), optimum catalyst fuction which are determined include of optimum pH, optimum temperature and optimum substrate concentration. The determination of specified activity of amylase are tested in optimum condition. The result of research are obtained the protein content are 2,933 mg/mL. The specified activity which are obtained from coconut’s buds (Coccos nucifera L.) are 80,376 U/mg in pH 6, temperature 50 ºC and substrate concentration 6,5 mg/mL   Keywords : amylase, coconut’s buds, dinitrosalicylate acid (DNS) and specified activity.
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM LIPASE DARI PERKECAMBAHAN BIJI DURIAN (Durio zibethinus L) Dzulkarnain, Dzulkarnain; Astuti, Winni; Sitorus, Saibun
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

This research was conducted to isolate and to know the optimum lipase working conditions (pH, temperature and concentration of substrate), and to know lipase activity from germination of durian seed (Durio zibethinus L.). Isolation is done by 2 stages namely, homogenization and centrifugation. Centrifugation is done at a speed of 12000 rpm with a temperature of 4°C for 30 minutes. Activity by using titration method and protein concentration using method. The optimum lipase temperature obtained was 60°C, optimum pH was 7, and the optimum substrat concentration was 1 % v/v. The lipase specific activity yielded 0,221 U/mg.   Keywords: lipase, Durio zibethinus L., Lipase specific activity.