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Keanekaragaman Burung di Kampus Uin Raden Intan Lampung

Biosfer: Jurnal Tadris Biologi Vol 9, No 2 (2018): Biosfer: Jurnal Tadris Biologi
Publisher : Universitas Islam Negeri Raden Intan Lampung

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Abstract

Biodiversity of Indonesia is worldwide known. One of those is bird biodiversity. The native utilize birds as a pet. It is because birds have a beautiful color and voice. Therefore, Rare birds is likely to be hunted to get their aesthetic value. Raden intan Islamic ngy (UIN RIL) were a part of city forest. Trees and scrub were considered as bird habitat. The aim of this research is to identify birds in UINRIL campus, gruping the bird based on their exctinction.This research was done in five station each three times. The method is point count. All data were collected at 06.00-09.00 and 15.00-18.00 WIB. Data were analyzing using deskriptif quantitative approach. Diversity index (H’) for all station is 1.166-2.351. Diversity average at all station were(H’) 2,023. This research had success write 24 species of birds from 16 famili. Three of them were under government surveillance pp no. 7 year 1999. They are cekakak sungai (Todirhamphus chloris), madu sriganti (Nectarinia jugularis), and madu kelapa (Anthreptes malacensis).

IDENTIFIKASI FRAGMEN DNA MITOKONDRIA PADA SATU GARIS KETURUNAN IBU DARI SEL EPITEL RONGGA MULUT DAN SEL FOLIKEL AKAR RAMBUT

Biosfer: Jurnal Tadris Biologi Vol 8, No 1 (2017)
Publisher : Pendidikan Biologi Fakultas Tarbiyah dan Keguruan

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Abstract

Abstrak: DNA mitokondria (mtDNA) manusia memiliki sejumlah sifat genetik khas yang membedakannya dari genom inti yang dimanfaatkan dalam identifikasi penurunan hubungan kekerabatan, studi evolusi dan migrasi global manusia modern, bidang forensik dan identifikasi penyakit genetik. DNA mitokondria (mtDNA) manusia memiliki laju mutasi yang lebih cepat dibandingkan dengan DNA inti sehingga dapat dimanfaatkan untuk pemeriksaan sampel yang terbatas seperti untuk kepentingan visum. Penelitian ini bertujuan untuk megidentifikasi fragmen DNA mitokondria dari lisis sel epitel rongga mulut menggunakan primer M1dan HV2R, dan sel folikel akar rambut mengunakan primer M1 dan M2 dengan teknik PCR dan elektroforesis agarosa.Sampel sel epitel mulut dan akar rambut yang diambil dari satu garis keturunan dilisis terlebih dahulu dan dilakukan ekstraksi sel secara kimiawi dan enzimatik sehingga DNA total dapat keluar dari inti sel dan mitokondria. Setelah itu dilakukan amplifikasi fragmen D-loop mtDNA secara in vitro dengan dua jenis primer yang berbeda menggunakan teknik PCR dan proses terakhir yaitu dilakukan analisis hasil PCR menggunakan teknik elektroforesis gel agarosa. Hasil penelitian menunjukkan bahwa amplifikasi fragmen DNA mitokondria menggunakan primer M1 dan HV2R dari sel epitel rongga mulut menghasilkan fragmen berukuran 1 kb. Pada hasil amplifikasi fragmen DNA mitokondria dari sel folikel akar rambut menggunakan primer M1 dan M2, fragmen yang didapatkan berukuran 0,4-0,5 kb. Dengan demikian, fragmen DNA mitokondria dapat diamplifikasi menggunakan dua primer balik M2 dan HV2R yang dirancang pada ujung daerah D-LOOP mitokondria.

IDENTIFIKASI FRAGMEN HV1 DNA MITOKONDRIA INDIVIDU DATARAN RENDAH DAN DATARAN TINGGI

Biosfer: Jurnal Tadris Biologi Vol 8, No 2 (2017)
Publisher : Universitas Islam Negeri Raden Intan Lampung

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Abstract

An individual adaptation towards the geographical altitude associated with genetic factors, one of the genetic materials of the most widely used to study the characteristic of individuals in the population is the mitochondrial DNA.Based on that condition, this research will deciding human Fragmen HVI mtDNA from Indramayu as lowland individual and Sukabumi as highland individual. The purpose of this research is to identify hair root and the epithelial cell of the mouth as a source DNA mitochondrial that can used for a amplification of human fragment DNA mitokondria template and to identify fragment territory HV1 mtDNA by using Polimerase Chain Reaction (PCR) technic and detecting the yield of PCR from lowland and highland individual. The steps include the lysis to do the sample of the hair root and the epithelial cell of the mouth, amplified HV1 fragment mtDNA to utilize Polimerase Chain Reaction (PCR) technique and the result of PCR with elektroforesis gel agarosa. This research has been successfully, hair root and the epithelial cell can do analyze DNA mitochondrial marked by the fragment measuring 0,4 kb in the area D-Loop mtDNA and it can easier amplification is hair root characterized by the presence of DNA bands in agarose gel electrophoresis.

ENDOGAMY MARRIAGE MITOCHONDRIAL DNA VARIATION OF NORTH CIGINTUNG GARUT ISOLATES

Biosfer: Jurnal Tadris Biologi Vol 9, No 1 (2018):
Publisher : Universitas Islam Negeri Raden Intan Lampung

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Abstract

Mitochondrial DNA (mtDNA) has an unique genetic sequences which made its different from nuclear DNA. MtDNA is maternally inherited without recombination, and it has higher mutation rate rather than nuclear DNA. D-loop is an noncoding area in mtDNA which has highest polymorphism among mtDNA. The objective of this research is to study about nucleotide variation in D-Loop area from people who has endogamy marriage. The subject is four generation of maternally inherited. The research steps are sample collection, DNA template preparation, mtDNA amplification, agarose gel electrophoresis, sequencing analysis and homology analysis. The amplification of D-Loop fragment has 0,9 kb in size. Homology analysis showed fifty varians nucleotides.