Haperidah Nunilahwati, Haperidah
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EKSPLORASI, ISOLASI DAN SELEKSI JAMUR ENTOMOPATOGEN PLUTELLA XYLOSTELLA (LEPIDOPTERA: YPONOMEUTIDAE) PADA PERTANAMAN CAISIN (BRASSICA CHINENSIS) DI SUMATERA SELATAN Nunilahwati, Haperidah; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 12, No 1 (2012): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Abstract

Plutella xylostella is the most destructive insect pests of the brassicae family. The research objective was to explore, isolate and select entomopathogenic fungi as biological agents for control of P. xylostella. This study used 20 fungal isolates originating from soil and infected insects around the farmers’ field in lowland and highland of South Sumatra. The fungal isolates were tested to third instar larvae of P.xylostella. The suspension of entomopathogenic fungus was topical inoculated with a density of 1x106 conidia ml-1 on the test insect and five replicates. The result showed that the highest (83%) and the lowest (41%) mortality of the larvae P.xylostella was induced by fungal BPluS and BNIPTr, respectively. Moreover, the shortest (2.1 days) and the highest (4.3days) lethal times of the infected host were induced by fungal BPluS and BNIPTr, respectively.
Uji Efikasi Bioinsektisida Jamur Entomopatogen Berformulasi Cair terhadap Plutella xylostella (L.) Di Laboratorium Nunilahwati, Haperidah; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia; Khodijah, .; Meidelima, Dewi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 1 (2013): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Abstract

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.
Efek Bionematisida terhadap Serangan Nematoda Puru Akar Meloidogyne incognita (Koffoid & White) Chitwood pada Beberapa Takaran Purwanti, Yani; Nunilahwati, Haperidah; Khodijah, Khodijah
Seminar Nasional Lahan Suboptimal 2018: Prosiding Seminar Nasional Lahan Suboptimal "Tantangan dan Solusi Pengembangan PAJALE dan Kel
Publisher : Seminar Nasional Lahan Suboptimal

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Abstract

Purwanti et al, 2019. Bionematicidal Effects on the Attack of Root-Knot Nematode Meloidogyne incognita (Koffoid & White) Chitwood at Several Doses. pp. 198-205.Biological control by utilizing antagonistic fungi is an alternative control of root-knot nematode (RKN) which does not damage the environment.  Giving organic material to the soil can increase soil fertility and support the success of biological control.  Bionematicides in the form of manure compost enriched with antagonistic fungi at the right dosage are expected to be able to control the attack of root-knot nematodes and increase tomato growth and production.  The aim of the study wass to determine the effect of bionematicide treatment on chicken manure compost enriched with antagonistic fungi from South Sumatra lowland vegetable crops at various doses in controlling the attack of root-knot nematodes on tomato plants. The bionematicide in the form of  chicken manure compost with Aspergillus 2 fungi and Trichoderma  has a high potential in suppressing root-knot nematode attacks.  Increased bionematicidal dose decreases the amount of nodule formed at the roots of tomatoes. Bionematisidal treatment in the form of chicken manure enriched with Aspergillus 2 and Trichoderma fungi at a rate of  300 g  10 - kg of soil produced the lowest amount of the purse on the root with the highest growth and tomato production.
KOLONI JAMUR ANTAGONIS Trichoderma spp PADA BEBERAPA MEDIA TUMBUH SECARA IN VITRO Nunilahwati, Haperidah; Purwanti, Yani; ., Khodijah; Nisfuriah, Laili; Rompas, Joni Philep
JURNAL TRIAGRO Vol 2, No 2 (2017): Volume 2 No.2 Juli – Desember 2017
Publisher : JURNAL TRIAGRO

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Abstract

Trichoderma is an antagonist biological agent in some fungal pathogens that cause many plant diseases in farmland. The effort to multiply Trichoderma fungi in vitro may use some media material. The appropriate medium is a prerequisite for studying microorganisms in different environments. The study aims to determine the development of Trichoderma fungal colonies in a macroscopic way in some media. The research was conducted in the laboratory of Plant Pest and Disease Faculty of Agriculture Palembang University from September to November 2017. The research was carried out by stages of preparation of isolates, media preparation and Trichoderma fungus inoculation on potato media, cassava media, sweet potato media, rice media, corn and green bean media. Observation of macroscopic colony development was done 6 days after inoculation (hsi), ie density and colony color, then identified, and data were analyzed descriptively. The results showed that Trichoderma fungi developed in potato media, cassava media, sweet potato media, rice media, corn media and green bean media. Trichoderma fungal colonies on potato media, sweet potato media, green bean medium is more dense than in cassava media, rice media and corn media. Trichoderma fungal colonies form a circle with clear boundaries, colony color was dark green and bright green with white side colonies on all media.Keywords: Fungal colonies, biological agents, Trichoderma, media, in vitro