Parawansah Parawansah, Parawansah
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Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah, Parawansah; Nuralifah, Nuralifah; Alam, Gemini; Natzir, Rosdiana
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

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BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages Parawansah, Parawansah; Nurtamin, Tomy; Mulyawati, Sufiah Asri; Nuralifah, Nuralifah; Misnaeni, Wa Ode Arlina
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

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BACKGROUND: The purpose of this research is to understand the secondary metabolites of Momordica charantia L. extract, as well as to disclose the potential of M. charantia extract in the phagocytic activity and capacity of peritoneummacrophages.METHODS: Examination of immunomodulatory effect was done by giving M. charantia ethanol extract on 5 treatment groups, given intra-peritoneally to mice daily. Echinacea extract as positive control and double distilled water as negative control were also given. On the 8th day, mice were infected with Staphylococcus epidermidis. After 30 minutes, peritoneum fluid was obtained to observe the activity and capacity of macrophage cells.RESULTS: The results showed significant phagocytic activity (p<0.05) at a concentration of 1,200 ppm compare to the other groups. Meanwhile the macrophage cell capacity was found statistically insignificant (p>0.05). The highest phagocytic activity was the group treated with 1,200 ppm (62%), significantly higher than other groups.CONCLUSION: The secondary metabolite content of M. charantia is alkaloids, flavonoids, tannins, saponins, and triterpenoids. The 1,200 ppm M. charantia ethanol extract is potential in inducing phagocytic activity and capacity. These results indicate that the M. charantia can be suggested as a natural immunomodulator.KEYWORDS: pare fruit, Momordica charantia L., phagocytosis, macrophage, immunomodulator
Uji Efek Antipiretik dan Antiinflamasi Ekstrak Etanol Buah Pare (Momordica charantia L.) terhadap Mencit Jantan Parawansah, Parawansah; Wahyuni, Wahyuni; Mahmudah, Zakiyatul
Medula Vol 4, No 1 (2016)
Publisher : Medula

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This research was carried out to determine the content of secondary metabolites contained in the ethanol extract of bitter melon (Momordica charantia L.) and antipyretic and antiinflammatory effects on mice. Bitter melon extract obtained by maceration method using ethanol 96%. Bitter melo ethanol extract was made in three variations of dose 50 mg / KgBW, 150 mg / KgBW, and 250 mg / KgBW. The content of secondary metabolites determined phytochemical Skrining by TLC (thin layer chromatography) with specific reagent. Antipyretic effect performed by vaccine DPT (Diphtheria Pertussis Tetanus) as inducers of fever and Parasetamol 500 mg as a positive control and antiinflammatory effect using the method of edema artificial on the soles of the mice foot with carrageenan 2% as an aphrodisiac edema and Diclofenac Sodium 50 mg as a positive control. Subjects used is a white male mice were given intraperitoneally (i.p). Analysis of the data using ANOVA statistical analysis and continued with LSD Post Hoc test. Phytochemical screening results showed that ethanol extract of bitter melon contains alkaloids, tannins, flavonoids, saponins, and triterpenoids. The results indicated that ethanol extract of bitter melon at dose of 150 mg/KgBW has antipyretic effect with total temperature decrease 1,1oC and anti-inflammatory effects with the largest of inflammation inhibition percent by 68.57%. One way ANOVA analysis signify a probability value (p) < 0.05 with 95 % of confidence level that indicate a significant influence of ethanol extract of bitter melon to its antipyretic and antiinflammatory effects on male mice.Keywords : Antipyretic, Anti-inflammatory, Momordica charantia, Bitter melon
Uji Antidiabetik Ekstrak Buah Pare (Momordica charantia L.) terhadap Penurunan Kadar Glukosa Darah pada Mencit yang Diinduksi Streptozotocin Parawansah, Parawansah; Nuralifah, Nuralifah; Kholidha, Andi Noor
Medula Vol 5, No 1 (2017)
Publisher : Medula

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ABSTRACT Diabetes mellitus is a chronic disease in which the patient has excess glucose levels in the blood. Pare (Momordica charantia L.) is one of the plants that have efficacy in treating various diseases one of them is antidiabetes. Activity of active substances from the pare may play a role in lowering blood sugar levels. This research is done to know the effect of increasing concentration of pare extract to decrease blood glucose level in mice induced by streptozotocin.This study was experimental laboratory, the study was done by measured  the initial blood sugar level in mice, sugar content after induction of steptozotosin, and after administration of 100 mg / kgBW extract test, 250 mg / kgBW and 400 mg / kgBW with observation time for 7 days , as well as gave metformin suspension as a positive control.The results showed that the optimum value of antidiabetic activity of ethanol extract of pare fruit at a concentration of 400 mg / kgBW and had an average decrease of 122 mg / dl.Keywords: Antidiabetes, Momordica charantia L., Streptozotocin
Uji Antioksidan Ekstrak Etanol Buah dan Biji Mengkudu (Morinda citrifolia L.) dengan Metode DPPH (1,1-diphenyl-2-picrylhydrazyl) Aritrina, Pranita; Parawansah, Parawansah; Ardani, Nurul
Medula Vol 5, No 1 (2017)
Publisher : Medula

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ABSTRACT Background: Free radicals and antioxidant were widely discussed in world of health because most of the diseases were initiated by presence of oxidation reaction in the body. The process of free radical formation could be inhibited by the presence of antioxidant. Antioxidant was a compound that can protect the biological system in the body. Research Purpose: This research aimed to analyze the potential of fruits and seeds of Morinda citrifolia L. as antioxidant which could counteract free radicals using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Research Method:This research used experimental method with post test only control design. The research was located in Integrated Laboratory of Faculty of Medicine and Pharmacy Laboratory of Faculty of Pharmacy of Halu Oleo University. Samples of this research were fruit and seed ethanol extract of Morinda citrifolia L.which were taken in West Kendari, Kendari City. The independent variables from this research were fruit ethanol extract and seed ethanol extract of cheese fruit and dependent variable was antioxidant potential. Potential and antioxidant activity (IC50) were tested by measuring DPPH (1,1-diphenyl-2-picrylhydrazyl) uptake using a spectrophotometer. Antioxidant activity (IC50) was analyzed using a linear regression equation. Research Result:The result of this research showed that there was a color changing of DPPH from purple to yellow on fruits ethanol extract and seeds ethanol extract of Morinda citrifolia L. and had antioxidant activity about IC50 398,99 ppm and 401,37 ppm, respectively.Conclusion: Conclusion from this research revealed that fruit ethanol extract and seed ethanol extract of Morinda citrifolia L.has weak antioxidant activity.Keywords : Antioxidant activity, DPPH method, IC50, Morinda citrifolia L.
Uji Efek Antidiabetik Ekstrak Daun Andong (Cordyline fruticosa L. A. Cheval) Mus musculus yang Diinduksi Streptozotosin Parawansah, Parawansah; Giatna, Sifak; Yusuf, Muhammad Ilyas
Medula Vol 2, No 2 (2015)
Publisher : Medula

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The plant andong leaf is a medicinal plant that has not been widely used and contains phenolic, flavonoids,tanins and saponins has many benefits, one of which lower blood glucose levels. This study aim to look at the effects of decrease in blood glucose levels in  mice that induced by STZs. The method used was experimental research design is RAL with treatment variations leaf extracts 15%, 30% and 45%, glibenclamid as positive control and Na. CMC negative control. The results were analyzed by ANOVA and continued with Duncan. The results showed that there is a very real difference in the treatment given, andong leaf extracts concentrations is themost effective extracts 15% with the average is 17,67 mg/dL on the hour to three.Key words : Leaf andong, antidiabetic, Mus musculus, streptozotocin.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia Linn.) TERHADAP LARVA UDANG (Artemia salina Leach) DENGAN METODE BRINE SHRIMPLETHALITY TEST (BSLT) Parawansah, Parawansah; Nuralifah, Nuralifah; Akib, Nurlliyin; Antrie, Geong
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Buas-buas (Premna serratifolia Linn.) belongs to the family Verbenaceae has been used as a traditional medicine. The aims of this research are to determine the compound, extract characteristics and potential toxicity (LC50) of ethanol extract of buas-buas leaves. The used method is Brine Shrimp Lethality Test (BSLT). The test consisted of six concentration treatments namely 1000, 750, 500, 250, 100 ppm and negative control with three replications. In each concentration used 10 Artemia salina leach larvae, and observations were made during 24 hours of larvae mortality. The content of phytochemical compounds from the buas-buas leaves are alkaloids, flavonoids, saponins and tannins. Characterization extract showed 62,21% contents of ethanol soluble extract, 41,5% contents of water soluble extract, 14,1% contents moisture and 4,1% contents ash. LC50 value was determined by probit analysis. The result of LC50 is 133,96 ppm. LC50<1000 ppm showed that the ethanol extract of buas-buas leaves as toxic.Kata kunci— Premna serratifolia Linn., Brine Shrimp Lethality Test (BSLT), LC50, Artemia salina Leach
Uji Toksisitas Akut Ekstrak Etanol Daun Notika (Archboldiodendron calosercium (Kobuski)) Terhadap Larva Artemia salina Leach dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Nuralifah, Nuralifah; Jabbar, Asriullah; Parawansah, Parawansah; Iko, Ria Agus
Pharmauho Vol 4, No 1 (2018): Pharmauho
Publisher : Pharmauho

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Daun notika merupakan tumbuhan yang berasal dari Papua, yang tumbuh di daerah perbukitan yang telah terjadi erosi.Daun notikasecara empiris memiliki manfaat menyembuhkan penyakit malaria dan mempermudah proses persalinan. Penelitian ini bertujuanuntuk mengetahui kandungan metabolit sekunder dari daun notika, karakterisasi simplisia daun notika dan toksisitas akutmenggunakan metode BSLT terhadap larva udang Artemia salina Leach. Ekstrak daun notika diperoleh dengan menggunakanmetode maserasi dengan pelarut etanol 96%. Pengujian skrining fitokimia dilakukan dengan metode perubahan warna yangmeliputi uji alkaloid, uji flavonoid, uji terpenoid, uji tannin dan uji saponin. Pengujian karakterisasi simplisia dilakukan denganmenentukan parameter spesifik yang meliputi penetapan sari larut air dan penetapan sari larut etanol sedangkan parameter nonspesifik meliputi penetapan kadar air dan penetapan kadar abu. Pengujian toksisitas akut menggunakan metode Brine ShrimpLethality Test (BSLT) dengan menggunakan larva udang Artemia salina Leach. Hasil pengujian skrining fitokimia daun notikamenunjukkan hasil positif pada flavonoid, terpenoid, saponin dan tanin. Hasil pengujian karakterisasi simplisia pada daun notikaadalah kadar sari larut etanol adalah 50,04%, kadar sari larut air adalah 32,01%, kadar air 3,99 % dan kadar abu adalah 5,11%.Hasil pengujian toksisitas akut dari ekstrak etanol daun notika secara BSLT menunjukan bahwa daun notika tidak bersifat toksikdengan nilai LC501024,19 µg/mL sehingga dapat dimanfaatkan sebagai obat tradisional.Kata kunci: daun notika, BSLT, toksisitas akut, Artemia salina, obat tradisional
Uji Aktivitas Antibakteri Sediaan Krim Antijerawat Ekstrak Etanol Terpurifikasi Daun Sirih (Piper betle L.) Dengan Basis Vanishing Cream Terhadap Propionibacterium acne Nuralifah, Nuralifah; Armadany, Fery Indradewi; Parawansah, Parawansah; Pratiwi, Aulif
Pharmauho Vol 4, No 2 (2018): Pharmauho
Publisher : Pharmauho

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AbstrakJerawat merupakan gangguan pada kulit yang ditandai dengan adanya peradangan yang disertai penyumbatan pada saluran kelenjar minyak dalam kulit yang disebabkan oleh bakteri Propionibacterium acne. Salah satu tanaman obat yang dapat dimanfaatkan sebagai obat jerawat adalah daun sirih. Daun sirih diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak dipurifikasi dari klorofilnya dengan metode ekstraksi cair-cair menggunakan pelarut n-heksan. Penelitian ini bertujuan untuk mengetahui efek antibakteri terhadap ekstrak etanol daun sirih terpurifikasi dan sediaan krim dengan basis vanishing cream terhadap bakteri P. acne dengan metode sumuran. Uji stabilitas fisik sediaan krim meliputi pemeriksaan organoleptis, homogenitas, daya sebar, viskositas, pH, dan tipe krim m/a.  Hasil penelitian menunjukkan bahwa ekstrak etanol daun sirih terpurifikasi dengan konsentrasi 0,5% memiliki daya hambat yang lemah, 1% memiliki daya hambat yang sedang, 1,5% dan 2% memiliki daya hambat pertumbuhan P. acne yang kuat. Konsentrasi ekstrak dalam pembuatan krim antijerawat dengan basis vanishing cream sebesar 0,5% memiliki daya hambat yang lemah, 1% dan 1,5% memiliki daya hambat yang sedang, 2% memiliki daya hambat yang kuat sedangkan kontrol positif (asam retinoat) yang digunakan memiliki daya hambat yang kuat terhadap pertumbuhan P. acne. Krim antijerawat dengan basis vanishing cream dengan konsentrasi ekstrak 2% memiliki aktivitas antibakteri yang paling baik terhadap P. acne.Kata kunci: Antibakteri, Daun Sirih, Krim Antijerawat Tipe m/a, Propionibacterium acne.