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Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum TRI-PANJI, .; SUHARYANTO, .; WIJAYANTI, Marini
E-Journal Menara Perkebunan Vol 77, No 1: Juni 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

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AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
KOMBINASI LARVA LALAT BUNGA (Hermetia illucens L.) DAN PELET UNTUK PAKAN IKAN PATIN JAMBAL (Pangasius djambal) Hariadi, Soikar; Irsan, Chandra; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 2, No 2 (2014): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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ABSTRACT The objective of study was to know the effect of combination of black soldier fly larva and pellet for growth and survival rate of Pangasius djambal and also to know the optimal food proportion combination of black soldier fly larva and pellet for growth and survival rate of P. djambal. The experiment was arranged has been done for 56 days in the Jambi Freshwater Aquaculture Development Center (JFADC). Fish that used were P. djambal with average weight of 10.17 g. They were placed in aquarium size 100x50x40 cm3 with the density 10 fish/aquarium. The experiment was arranged in a completly randomized design (CRD) with five treatments. Food combination tested were as follows : P1 (100% pellet), P2 (75% pellet and 25% black soldier fly larva), P3 (50% pellet and 50% black soldier fly larva), P4 (25% pellet and 75% black soldier fly larva), P5 (100% black soldier fly larva). The measured parameters were daily growth rate, food convertion ratio, protein efficiency ratio and survival rate of P. djambal. The result of this current experiment indicated that combination of black soldier fly and pellet influenced daily growth rate, food convertion ratio, protein efficiency ratio, but not influenced survival rate of P, djambal. The optimal food combination proportion wich gave the best daily growth rate, food convertion ratio, protein efficiency ratio was 97.0-82.5% pellet and 17.5-21.0% black soldier fly larva. The survival rate of P. djambal for all treatment was 100%. Keywords: Pangasius djambal, growth, survival rate, black soldier fly larva, feed
PERTUMBUHAN POPULASI Spirulina platensis DALAM MEDIA LIMBAH CAIR BAHAN OLAHAN KECAP DAN MEDIA ZARROUK Tinambunan, Junedi; Wijayanti, Marini; Jubaedah, Dade
Jurnal Akuakultur Rawa Indonesia Vol 5, No 2 (2017): JARI VOLUME 5 NO 2 DESEMBER 2017
Publisher : Universitas Sriwijaya

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ABSTRACTThe aims of this research were to study the influence of mixed industrial soy sauce liquid waste and Zarrouk medium mixture on population density and specific growth rate of Spirulina platensis on a short periode. This study has been conducted from March to April 2017 in the Laboratory of Aquaculture, Aquaculture study Program, Faculty of Agriculture, University of Sriwijaya. This research was arranged according to completely randomized design (CRD) with 5 treatment levels and 3 replications. The treatment levels consisted of P1 (0 % industrial soy sauce liquid waste + 100 % Zarrouk medium), P2 (25 % industrial soy sauce liquid waste + 75 % Zarrouk medium), P3 (50 % industrial soy sauce liquid waste + 50 % Zarrouk medium), P4 (75 % industrial soy sauce liquid waste + 25 % Zarrouk medium), P5 (100 % industrial soy sauce liquid waste + 0 % Zarrouk medium). The parameters observed during the study were population density and the maximum specific growth rate. The result of this study showed that the treatment P2 gave the best in maximum density (60.6 g.L-1 ) and specific growth rate (14.66%. day-1). Keywords: Spirulina platensis, the liquid waste ketchup, Zarrouk media.
Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum TRI-PANJI, .; SUHARYANTO, .; WIJAYANTI, Marini
E-Journal Menara Perkebunan Vol 77, No 1: Juni 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Original Source | Check in Google Scholar | Full PDF (374.392 KB)

Abstract

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
PENAMBAHAN SARI BUAH BELIMBING WULUH (Averrhoa bilimbi) PADA PAKAN UNTUK MENGOBATI IKAN LELE SANGKURIANG (Clarias sp.) YANG DIINFEKSI Aeromonas hydrophila Agustina, Herdia; Sasanti, Ade Dwi; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 5, No 2 (2017): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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ABSTRACT Motile Aerhomonas Septicemia (MAS) is one of bacteria diseases which is often attack freshwater fish. Motile Aerhomonas Septicemia (MAS) or hemmorage septicemia caused by bacteria A. hydrophila. The aim of this reserch examine Averrhoa bilimbi juice mixed on feed in order to effect sangkuriang catfish by A. hydrophila. The research was conducted at September until October 2016 in Laboratorium Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya and Laboraturium UPT Klinik Universitas Sriwijaya. Method of the research uses Completely Ranomized Design with six treatments and three replications. This research was conducted for 25 days of rearing. The catfish given feed mixed A. b          ilimbi juice about 150 mL.kg-1 (P1), 200 mL.kg-1 (P2), 250 mL.kg-1 (P3) dan 300 mL.kg-1 (P4) with frequency was three times a day. The parameters of this reserch is wound diameter, the percentage of cured fish, hematocrit, growth, food convertion ratio, survival rate, and chemical properties (temperature, pH, DO and ammonia). The results showed that the addition of A. bilimbi juice at P1 be obtained the percentage of cured fish was 64.47% and survival rate was 71.11%. P2 be obtained the percentage of cured fish was 68.89% and survival rate was 73.33%. P3 be obtained the percentage of cured fish was 72.53% and survival rate was 80.00%. P4 be obtained the percentage of cured fish was 95.40% and survival rate was 95.56%. P4 with dose 300 mL.kg-1 feed is the best treatment bacterial A. hydrophila infection of hematocrit was 32%, the percentage of cured fish was 95.40%, and survival rate was 95.56%. Water quality parameters during maintenance is still in a state of optimum range. Keywords: Aeromonas hydrophila, Sangkuriang catfish, Averrhoa bilimbi juice.
Bacterial Communities in Aquatic Sediment from Bukit Duabelas Raintforest and Oil Palm Plantation at Sumatra Indonesia Wijayanti, Marini; Wahyudi, Aris Tri; Yuhana, Munti; Engelhaupt, Martin; Meryandini, Anja
HAYATI Journal of Biosciences Vol 25, No 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

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The diversity of bacterial communities in aquatic sediment of rainforest and oil palm plantation at Sumatra was studied using pyrosequencing of 16S rRNA gene and common biodiversity indices.  Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas.  Ecological approach used soil pH, total Carbon (TC), total Nitrogen (TN), available Phosphorus (AP) measurement and bacterial diversity with Shanon and Simpson indices, and bacterial richness with Chao1-ACE indices and OTUs.  Bacterial diversity and richness in aquatic sediment of forest area was higher than that ones in oil palm plantation area, although their pH, TC, and TN in both areas were not different significantly. The majority of sequences related to Proteobacteria (34.85%), Acidobacteria (32.67%), Nitrospirae (6.86%), Chloroflexi (4.31%), and Actinobacteria (4.02%) were from forest; whereas those related to Acidobacteria (46.10%), Proteobacteria (25.86%), Nitrospirae (9.20%), Chloroflexi (4.99%), and Actinobacteria (2.34%) invented from oil palm plantation. The genera of Alphaproteobacteria and Betaproteobacteria dominated in genera phylotype of bacterial 16S rRNA phylogenetic revealed both aquatic sediment of forest and oil palm plantation. The most genera in the phylogenetic tree from aquatic sediment of both areas was Burkholderia.  The bacterial community shift in aquatic sediment of forest transformation indicated higher bacterial diversity index, richness index, some of phyla and genera in aquatic sediment from forest than from oil palm plantation.
MODIFIKASI MEDIA SPIRULINA PLATENSIS SEBAGAI UPAYA PEMANFAATAN AIR LIMBAH BUDIDAYA IKAN LELE Widyantoro, Hendro; Wijayanti, Marini; Dwinanti, Sefti Heza
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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ABSTRACTThe purpose of this study was to determine the best fertilizer compotition that was made from technical fertilizer and waste water of cat fish farming. This experimental was conducted at Aquaculture Laboratory, Faculty of Agriculture, Sriwijaya University. It used complete randomized design (CRD) method which had five treatments and three replications, the waste water from catfish pond (P0), waste water from catfish pond + 25% technical fertilizer (P1), waste water from catfish pond + 50% technical fertilizers (P2), the waste water from catfish pond + 75% technical fertilizers (P3), the waste water from catfish pond + 100% technical fertilizers (P4). The results showed that the best maximum density of S. platensis was P3 about 3.98 g l-1, and growth rate was equal to 2.80% day-1. The most significant parameter of water quality was ammonia which decreased until 82.78%. Besides, it was more beneficial than others based on Return Cost Ratio (R/C ratio) and Benefit Cost Ratio (B/C ratio) which were 3.66 and 2.66 respectively. Keywords: Spirulina platensis, waste water pond of catfish, modified  media
Optimization of Spirulina platensis Culture Media as an Effort for Utilization of Pangasius Farming Waste Water Wijayanti, Marini; Jubaedah, Dade; Gofar, Nuni; Anjastari, Devi
Sriwijaya Journal of Environment Vol 3, No 3 (2018): Sustainable Resources
Publisher : Program Pascasarjana Universitas Sriwijaya

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Pangasius is a fast-growing fish species that has great potential for production and export growth in Indonesia. Their farming produces a lot of organic material and ammonia which potentially make polution in freshwater body. The wastewater can be used for high value microalgal cultivation media.  The microalgae are used in various fields, one of those is Spirulina platensis, a spiral blue green algae.  This aims of this study was to determine the best composition of the technical fertilizer in the pond waste of Pangasius farming pond to obtain maximum density and know the spesific growth rate of Spirulina platensis. The research method used Completely Randomized Design (CRD) with 6 treatments (3 replication), P0 using 100% technical fertilizer without using Pangasius waste water while P1-P5 use 0% (P1), 25% (P2), 50% (P3), 75% (P4) and 100% (P5) technical fertilizer using Pangasius farming waste water. The most efficient treatment obtained 23.90 gL-1 maximum density, 6.22%.d-1 specific growth rate and 87.77% ammonia removal.
Pengaruh Penambahan Pupuk Organik Cair terhadap Kesuburan Tanah dan Kualitas Air pada Kolam Tanah Rawa Pasang Surut di KTM Telang Banyuasin Dewi, Santa; Jubaedah, Dade; Wijayanti, Marini; Anggraini, Septika Putri
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 8, No 1 (2019): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Dewi et al, 2019. The Effect of Addition of Liquid Fertilizer on Soil Fertility and Water Quality in Pond at Tidal Lowland. JSLO 8(1):The soil of tidal lowland contains a lot of sulfidic material, low soil pH (> 3.50), high toxic substances and low soil fertility. This study aimed to compare the effect of adding liquid fertilizer to soil ponds is made of afkir fruit on tidal swamp land. The research used three treatments without replication. The treatments were pond without liquid fertilizer/control (P0), addition of liquid fertilizer concentration 0.131 L.m-3 (P1) and 0.261 L.m-3 (P2). The parameter were water quality, soil fertility, abundance and composition of plankton, and plankton that were potensial as natural feed of fish. The data obtained processed using descriptive analysis. The result of this research indicated that the addition of liquid fertilizer in KTM Telang Banyuasin gave positive changes to water quality and soil fertility parameters such as pH, secchi depth transparency, ammonia, orthophosphate, iron, N-total, P-available, K-dd, C-organic and pyrite, and has a negative effect on dissolved oxygen.. The treatment P2 resulted in highest plankton abundance value of phytoplankton (7974 ind.L-1) and zooplankton (192 ind.L-1), the fertility of pond was on moderate level (mesotrophic), 100% of phytoplankton natural food potency and 71.4% of zooplankton natural food potency.  
Penggunaan Melati Air (Echinodorus palaefolius) sebagai Filter Biologi pada Pemeliharaan Ikan Maanvis (Pterophyllum scalare) Riyadhi, Kezia Agustine; Jubaedah, Dade; Wijayanti, Marini; Almaniar, Siska
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 8, No 1 (2019): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Almaniar et al, 2019. The Using of Water Jasmine (Echinodorus palaefolius) as Biological Filter on Angelfish (Pterophyllum scalare)  Rearing. JLSO  Water quality was  One factors that become a contraint in ornamentalfish culture. This study aimed to compare the result of  jasmine water as a biological filter in maintaining water quality and reducing organic matter in the rearing of angelfish with a recirculation system. This research was conducted for 28 days at the Basic Laboratory of  Aquaculture, Departement of aquaculture, Universitas Sriwijaya. The research Used a completely randomized design with 5 treatments and 3 replications. The results showed that water quality  of  the temperature, pH, dissolved oxygen, ammonia, nitrat and  phosphate values was (27.2-29.2ºC), (5.7-6.9), (4.68-5.74 mgL-1), (0.005-0.037 mgL-1), (0,06-0,12 mgL-1) and ( 0.013-0.107 mgL-1). In treatment P0 the lowest fish survival was 44.45%, absolute length growth was 1.56 ± 0.26 cm, absolute weight growth was 1.47 ± 0.34 g, feed efficiency was 40,54 ± 2,54. While in treatment P4 the highest survival was 72.22%, absolute length growth was 1.24 ± 0.17, absolute weight growth was 1.25 ± 0.24 g feed efficiency 51.32 ± 13. The survival rate of angelfish in the treatment  without using water jasmine showed the lowest percentage of survival compared to other treatment.