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PERKEMBANGAN KULTUR IN VITRO PADA TANAMAN KARET (Hevea brasiliensis, Müell. Arg.) MELALUI EMBRIOGENESIS SOMATIK DI CIRAD PERANCIS Bintarti, Ari Fina
Warta Perkaretan Vol 34, No 1 (2015): volume 34, Nomor 1, Tahun 2015
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (990.143 KB) | DOI: 10.22302/ppk.wp.v34i1.58

Abstract

Embriogenesis somatik menjadi alternatif perbanyakan klonal pada tanaman karet untuk mendapatkan bahan tanam dengan sistem perakaran sendiri dan identik dengan induknya. Penelitian mengenai embriogenesis somatik pada tanaman karet sudah banyak dilakukan oleh lembaga penelitian di dunia, salah satunya adalah lembaga penelitian swasta CIRAD Perancis. Berbagai penelitian dalam upaya pengembangan metode embriogenesis somatik tanaman karet telah diteliti mulai tahun 1970-an, meliputi Embriogenesis Somatik Primer, Embriogenesis Somatik Jangka Panjang atau, dan Embriogenesis Somatik Sekunder. Selain itu, tim peneliti di CIRAD juga telah berhasil mengembangkan teknologi kriopreservasi atau penyimpanan jangka panjang di dalam nitrogen cair yang dikaitkan dengan Embriogenesis Somatic Sekunder untuk menyeleksi dan menetapkan galur-galur kalus yang mempunyai kapasitas embriogenik dan regenerasi tinggi. Walaupun begitu, teknik embriogenesis somatik belum bisa diaplikasikan secara luas untuk perbanyakan tanaman karet secara massal, karena beberapa kendala. Lebih jauh, teknik embriogenesis somatik dan kriopreservasi dapat mendukung program pemuliaan tanaman untuk menciptakan tanaman karet transgenik dengan sifat-sifat agronomis unggul seperti toleran terhadap stress abiotik dan produksi lateks tinggi.
Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Bintarti, Ari Fina; Rusmana, Iman; Wahyudi, Aris Tri
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/97

Abstract

Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3 and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indica ATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16 SrRNA indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24.