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PENGARUH PEMBERIAN HORMON METHYLTESTOSTERONE PADA LARVA IKAN GUPPY (Poecilia reticulata) TERHADAP PERUBAHAN JENIS KELAMIN

ZOO INDONESIA Vol 17, No 2 (2008): November 2008
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Huwoyon, G.H., Rustidja & R. Gustiano. 2008. Effect of methyltestosterone hormoneon sex reversal of Guppy (Poecillia reticulate) larvae fish. Zoo Indonesia 17(2): 49-54. Male ornamental fish is more exotic than female one. The objective of study wasto increase the percentage of male guppy fish (Poecillia reticulate) usingmethyltestosterone hormone at the larvae stage. Control (A = 0 ppm) and four differentdosages of methyltestosterone hormone (B = 5 ppm, C = 10 ppm, D = 15 ppm & E =20 ppm) were used as treatments with three replications. The results showed the besttreatment to produce the highest percentage (58.41%) of male guppy fish was at 10ppm dosage (p<0.01). However, the dosages hormone treatments given in this studywere not significantly different on intersexes production, survival rate and specificgrowth rate (p>0.05).

RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements]

BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.

ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)]

BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.

OPTIMALISASI KEPADATAN BENIH IKAN MAS (Cyprinus carpio) STRAIN RAJADANU PADA PENDEDERAN DI KOLAM AIR TENANG [Density Optimization of Carp Seed (Cyprinus carpio) Strain Rajadanu in the Nursery in Calm Pool Water]

BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

National fisheries production is not proportional to the increase of the population now, so that the consumption of animal protein needs are not met.For the sake of national fisheries production there is a need for intensive cultivation technology breakthroughs to spur the density in nursery phase ‘rajadanu’ carp (Cyprinus carpio) strain. This experiment aim to determine the appropriate density of rajadanu carp growth in the pond at the nursery phase. The average length of tested fishes is 2.02 cm and the average initial weight is 0.25 g maintained in the out door plot pool of Research Installation for Germ Plasm of the Research and Development Division of Freshwater Fisheries, Cijeruk, Bogor measuring 1x1x1 m with density 100, 150 and 200 fishes/m3 .Each treatment was repeated 3 times. Feed used was a commercial feed containing 28% protein given in 2 times a day as much as 5% of the total weight of fish. The results showed that after 40 days, each treatment showed no significant difference (P> 0.05) with absolute length and weight growth of the absolute highest in nursery density of 200 fishes/m3 of 1.30 ± 0.10 cm and 1.14 ± 0, 18 cm with a daily growth rate of 1.30 ± 0.10% and 4.08 ± 0.55%, and the highest survival at densities 200 fishes/m3 of 92.5 ± 6.55%.

PEMBESARAN IKAN BAUNG {Hemibagrus nemurus) YANG DIBERI PAKAN BERBEDA DIKOLAM TANAH

BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Green catfish is a commodity that is not only potential to be cultivated in the pond and in the floating net cages, but also easy to adjust to the artificial feed. The objective of this research is to evaluate the effect of different feed types (sinking and floating)on the growth performance of green catfish(Hemibagrus nemurus.One thousand and six hundred fingerlings averaging 2.50 g of 3 individual body weight were stocked in each of earthern ponds (10 x 8 x 1.2 m ). They were fed daily for four months with the same protein content (31%) and lipid (6%) diets. The feed was given in pelleted form at 8-4% of the total body weight. The result of this study showed that no significant difference (P>0.05) between different feed types. The sinking feed gave specific growth rate (2.35%), feed conversion ratio (2.49), protein retention (18.92%) and protein efficiency ratio 1.13 while the floating type gave the values 2.49%, 2.37, 20.48% and 1.21, respectively. Lipid retention of sinking feed (34.84%) is lower than that of floating type(48.39%).The survival rates ranged between 76.56-84.54%.Feed type sinking with protein content of 31% can be used in the rearing of fish in earthern pond.

LIMBAH BIOGAS KOTORAN AYAM SEBAGAI ALTERNATIF SUMBER PROTEIN PAKAN BENIH IKAN PATIN (Pangasianodon hypophthalmus)

Jurnal Sains Natural Vol 1, No 1 (2011): Sains Natural
Publisher : Universitas Nusa Bangsa

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Abstract

Chicken Manure Biogas Waste as Protein Source for Patin FryF.         The hight of  production cost encourage to search for  alternative low price feed. The objectives of the study was to know an appropriate substitution by chicken manure biogas protein for soybean meal protein, and the influence on growth performance and feed efficiency of siames catfish (Pangasianodon hypophthalmus). The treatments of chicken manure biogas protein  consisted of control (A = 0 %) and four treatment (B = 8 %, C = 16%, D = 24 % dan E = 32 %). The results showed that best chicken manure biogas protein was found in treatments C (16 %), with survival rate of 100 %, specific growth rate (3,4% bw/day), feed conversion ratio (1,0), retention of energy (30,3 %), protein efficiency ratio (3,6 g/g) and 47,8 % of protein retention. Conclusion showed that the chicken manure biogas protein could be used to substitute for soybean meal with the best result in treatment of feed C = 16 %.Keywords : biogas waste, chicken manure, feed, siames catfish, pangasianodon hypophthalmusABSTRAK         Tingginya biaya produksi yang menyebabkan mencari alternatif agar pakan murah. Penelitian ini bertujuan untuk mencari substitusi yang tepat antara protein limbah biogas kotoran ayam terhadap protein bungkil kedelai, serta pengaruhnya terhadap pertumbuhan dan perbandingan efisiensi pakan benih ikan patin (Pangasianodon hypophthalmus). Perlakuan pakan substitusi protein limbah biogas kotoran ayam terhadap protein bungkil kedelai terdiri atas kontrol (A = 0%) dan 4 perlakuan (B = 8 %, C = 16%, D = 24 % dan E = 32%). Hasil penelitian menunjukan bahwa substitusi terbaik pada protein limbah biogas kotoran ayam terhadap protein bungkil kedelai didapatkan pada perlakuan pakan C = 16 %, dengan nilai sintasan 100 %, laju pertumbuhan spesifik 3,4 % bt/hari, rasio konversi pakan 1,0, retensi energi 30,3 %, rasio efisiensi protein 3,6 g/g dan retensi protein 47,8 %. Dari data dapat ditarik kesimpulan bahwa limbah biogas kotoran ayam dapat dimanfaatkan sebagai substitusi bungkil kedelai dengan hasil terbaik pada perlakuan pakan C = 16 %.Kata kunci : limbah biogas, kotoran ayam, pakan, patin, pangasianodon hypophthalmus

ANALISIS VARIASI GENOTIPE IKAN KELABAU (Osteochilus kelabau) DENGAN METODE MITOKONDRIA-RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)

Jurnal Riset Akuakultur Vol 5, No 1 (2010): (April 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Abstract

Ikan kelabau (Osteochilus kelabau) adalah salah satu jenis ikan ekonomis penting di perairan Kalimantan yang populasinya menurun. Penelitian ini bertujuan untuk menganalisis variasi genotipe ikan kelabau dengan metode DNA mitokondria-Restrictrion Fragment Length Polymorphism (RLFP) sehingga diperoleh informasi tentang status genotipe ikan kelabau sebagai bahan masukan dalam penentuan pengelolaan ikan tersebut. Hasil menunjukkan bahwa ikan kelabau dari empat lokasi di daerah Kalimantan Barat memiliki variasi genotipe yang rendah dengan komposit haplotipe 0-0,189. Tiga komposit haplotipe terdeteksi dengan menggunakan 5 enzim restriksi yaitu Rsa I, Hae III, Taq I, Hin6 I, dan Alu I pada sekuens 16S-rRNA. Perbedaan yang nyata terlihat antara populasi Sintang dengan populasi lainnya. Jarak genetik terdekat adalah antara populasi ikan kelabau dari Pontianak dengan populasi ikan dari Sekadau, sedangkan jarak genotipe terjauh  adalah antara populasi ikan kelabau dari Sintang dengan populasi ikan dari Pontianak dan Sekadau dan ternyata ikan kelabau dari Sintang berbeda spesies dengan yang dari lokasi lainnya

PENGARUH KEPADATAN TERHADAP SINTASAN, PERTUMBUHAN, DAN GAMBARAN DARAH BENIH IKAN BETUTU Oxyeleotris marmorata

Jurnal Riset Akuakultur Vol 12, No 4 (2017): (Desember 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Abstract

Ikan betutu Oxyeleotris marmorata merupakan ikan lokal potensial menjadi komoditas budidaya. Performa pertumbuhan dan sintasan dapat ditingkatkan dengan mengoptimalkan padat tebar. Tujuan penelitian adalah menentukan padat tebar yang menghasilkan sintasan dan pertumbuhan tinggi, serta respons fisiologis terbaik. Kolam yang digunakan berukuran 2 m x 1 m x 1 m dan diisi air 1 m3. Perlakuan yang diuji adalah kepadatan 50 ekor/m3, 100 ekor/m3, dan 150 ekor/m3. Ukuran benih yang digunakan 4,24 ± 0,58 cm dengan bobot 2,74 ± 0,45 g. Selama 60 hari masa pemeliharaan, pakan yang diberikan adalah cacing sutra Tubifex sp. secara sekenyangnya. Hasil penelitian menunjukkan sintasan benih ikan betutu yang dipelihara pada berbagai padat tebar tidak berbeda secara nyata, pertumbuhan spesifik panjang (1,50 ± 0,37%/hari) dan bobot total benih ikan betutu (1,95 ± 0,32%/hari) tertinggi, dan perubah respons fisiologis berupa gambaran darah paling stabil dicapai pada padat tebar 50 ekor/m3, serta biomassa tertinggi dicapai pada kepadatan 150/m3. Hasil penelitian ini dapat dijadikan informasi dasar untuk melakukan pendederan ikan betutu secara terkontrol.Sand goby, Oxyeleotris marmorata is a potential fish species for aquaculture in Indonesia. However, the growth performance and survival rate of the fish seed are still low. Such challenges could be solved through the optimization of stocking density of the fish. The research objective was to determine the optimal stocking density to produce high growth and survival rate, as well as the best physiological response. The ponds used in this experiment were 2 m x 1 m x 1 m in size (water volume: 1 m3). The stocking density treatments were 50, 100, and 150 individual/m3. The initial fish length average was 4.24 ± 0.58 cm, with the initial body weight average of 2.74 ± 0.45 g. During 60 days of rearing period, the fish were fed with Tubifex sp. ad libitum. The results showed that the survival rates on different stocking densities were not significantly different. The highest specific growth on length (1.50 ± 0.37%/day) and body weight total (1.95 ± 0.32%/day) and the most stable physiological response related to its hematological parameters were achieved by seed stocked at 50 individuals/m3. The best biomass total was achieved by seed stocked at 150 individuals/m3. The result of this study could be applied as basic information to culture sand goby in a controlled environment.

PENINGKATAN PRODUKTIVITAS BUDIDAYA IKAN DI LAHAN GAMBUT Gleni Hasan Huwoyon dan Rudhy Gustiano

Media Akuakultur Vol 8, No 1 (2013): (Juni 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Indonesia merupakan negara dengan kawasan gambut tropika terluas di dunia, berkisar antara 13,5-26,5 juta ha (rata-rata 20 juta ha). Luas area gambut tersebut merupakan 50% gambut tropika dunia. Untuk mengoptimalkan potensi lahan gambut melalui perikanan diperlukan suatu strategi untuk menanggulangi masalah pH rendah. Pilihan strategi yang dapat diterapkan melalui pendekatan biologis adalah dengan memanfaatkan secara optimal ikan lokal yang telah beradaptasi dengan lingkungan tersebut selain itu, strategi pendekatan secara biologis lainnya adalah dengan melakukan introduksi ikan-ikan dari luar dan hasil rekayasa yang tahan terhadap perairan gambut. Beberapa jenis ikan yang telah diintroduksi dan mulai dikembangkan di perairan gambut adalah ikan patin siam (Pangasianodon hypophthalmus), lele dumbo (Clarias gariepienus), dan ikan nila (Oreochromis niloticus) strain BEST (Bogor Enhanched Strain Tilapia). Untuk mendukung pengembangan budidaya yang berkelanjutan diperlukan adanya pemahaman pengelolaan lingkungan yang benar di perairan gambut. Untuk mengoptimalkan potensi yang ada perlu adanya transfer teknologi dalam pengembangan ikan-ikan yang berpotensi untuk dibudidayakan di perairan gambut agar kesejahteraan dan pembangunan daerah khususnya berbasis perikanan dapat tercapai.

BEBERAPA JENIS IKAN LOKAL YANG POTENSIAL UNTUK BUDIDAYA: Domestikasi, Teknologi Pembenihan, dan Pengelolaan Kesehatan Lingkungan Budidaya

Media Akuakultur Vol 7, No 1 (2012): (Juni 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Biodiversitas ikan-ikan air tawar lokal asli Indonesia sangat berlimpah namun belum banyak dimanfaatkan dalam budidaya. Pemanfaatan secara langsung masih dalam taraf penangkapan di alam yang dikhawatirkan dapat membahayakan populasinya di alam. Salah satu alternatif untuk pencegahannya adalah dengan upaya meningkatkan budidaya dan mengurangi penangkapan yang berlebihan. Langkah pertama yang dibutuhkan adalah melakukan domestikasi untuk mendapatkan teknologi pembenihannya dan upaya pengelolaan lingkungan yang dibutuhkannya. Sekitar 20 jenis ikan lokal asli Indonesia telah dimanfaatkan dalam budidaya ikan konsumsi, sedangkan teknologi yang telah berhasil dikembangkan tercatat pada 7 jenis ikan air tawar seperti ikan baung, jelawat, nilem, kancra, tawes, belida, dan betutu.