Dian Mulawarmanti, Dian
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Effect of oxygen hyperbaric therapy on malondialdehyde levels in saliva of periodontitis patients with type 2 diabetes mellitus Mulawarmanti, Dian; Widyastuti, Widyastuti
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 4 (2008): (December 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Lipid peroxidation (LPO) has implication in pathogenesis of several pathological disorders including periodontitis. Malondialdehyde (MDA) is end products of upid peroxidation. Hyperbaric Oxygen Therapy (HBOT) involves the administration of 100% oxygen under atmosphere pressure and has been used as an adjuvant therapy, while saliva is a diagnostic tool for many oral and systemic diseases. Purpose: The aim of this study was to examine the effect of HBOT on malondialdehyde in saliva to measure lipid peroxidation in periodontitis patients with type 2 Diabetes Mellitus (DM). Methods: Eight regulated type 2 DM subjects were compared to ten unregulated periodontitis patients type 2 DM (n = 18). Pre HBOT and after 10 days HBOT with 2.4 ATA dose, unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 minutes and were then stored at −80° C until analyzed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. Results: Data showed that regulated type 2 DM had lower level of MDA (3.08 ± 0.62 ug/mol) compared with unregulated periodontally- type 2 DM subjects (5.88 ± 1.04 ug/mol) (p>0.05). MDA levels were significantly lower after HBOT in regulated DM (2.30 ± 0.46 ug/mol) compared with unregulated periodontally type 2 DM (4.09 ± 0.77ug/mol) (p < 0.05). The regulated DM subjects and post HBOT showed MDA levels lower than the periodontally-unregulated group significantly. Conclusion: The saliva of periodontitis patients with unregulated type 2 DM showed more lipid peroxidation than regulated DM type 2. HBOT decreased MDA levels in regulated and unregulated type 2 DM with periodontitis.
Ekstrak daun mangrove (A.marina)mempercepat kesembuhanulkus traumatikus (Extract of mangrove leaf (A.marina) accelerates the healing of traumatic ulcer) Mendrofa, Arvian Novanolo; S, Isidora Karsini; Mulawarmanti, Dian
Journal of Dentomaxillofacial Science Vol 14, No 1 (2015): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

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Abstract

Mangrove leaf (Avicennia marina) has a role in wound healing process, but its effect in wound healing in oral mucosa has not been researched yet. This study aimed to prove the effect of A.marina extract against traumatic ulcer and itseffective concentration in traumatic ulcer healing. The subjects of this research are 25 wistar rats that were randomized into 5 different groups; K0 control, K1 were given hyaluronic acid, P1 were given extract of A.marina10%, P2 were given extract of A.marina 20%, and P3 were given extract of A.marina 40%. After the subjects werewounded using amalgam stopper that has been heated before, they were given topical application once daily untilseven days. The ulcer diameter was measured at the second day and day 8 using caliper digital. The data, analyzedusing Kruskal-Wallis test showed that there’s difference in diameters between two groups (p&lt;0.05). The averagediameter differences of traumatic ulcer among rats are K0 = 0.5700 mm, K1 = 0.8380 mm, P1 = 0.7240 mm, P2 =0.8440 mm, and P3 = 0.9500 mm. It was concluded that A.marina extract of 10%, 20% and 40% concentrations haveeffect in the healing of traumatic ulcer, and 40% is the most effective concentration against traumatic ulcer.