Jenny Sunariani, Jenny
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VEGF expression and new blood vessel after dental X-ray irradiation on fractured tooth extraction wound Woroprobosari, Niluh Ringga; Sunariani, Jenny; Astuti, Eha Renwi
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 3 (2015): (September 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i3.p159-164

Abstract

Background:Dental X-ray has an important role in dentistry. Complication case such as tooth fracture extraction requires this examination to determine the appropriate treatment measures. Dental X-ray can also cause a negative impact to the body at cellular and even molecular level. Purpose: The aim of this study was to evaluate the decrease of vascular endothelial growth factor (VEGF) expression and new blood vessels number caused by dental X-ray irradiation on fractured tooth extraction wound on day 3 and 7 after extraction. Method: We used 30 wistar rats which was randomly divided into 6 groups. Each rat’s central insisive of left mandible was fractured and then extracted after or without X-ray irradiation. Group KA and KB were control groups without irradiation. Group P1 A and P1 B were treatment groups with 0.08 mSv irradiation dose. Group P2 A and P2 B were treatment groups with 0.16 mSv irradiation dose. The subject from group KA, P1 A, and P2 A were sacrficed and sockets were collected at day 3. The subject from group KB, P1 B, and P2 B were sacrficed and sockets were collected at day 7. Socket were processed and painted with hematoxylin eosin and immunohistochemistry, then observed with a microscope. Data processing was performed with SPSS 16 through one way anova test and post hoc Tukey test HS. Result: The lowest means expression of VEGF and the number of new blood vessels on the day 3 was found in P2 A group, and the highest found in the KA group. The lowest means expression of VEGF and the number of new blood vessels on the day 7 was found in P2 B group, and the highest found in the KB group. Conclusion: Dental X-ray irradiation dose of 0.08 mSv and 0.16 mSv causes decrease of VEGF expression and new blood vessels in the wound fractured tooth extraction in day 3 and day 7 post-extraction.
Effect of IL-1 and gustducin expression change on bitter taste during fever Sunariani, Jenny
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 2 (2008): (June 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v41.i2.p95-99

Abstract

Homeostatic changes in the body, such as fever, cause inflammation, whose one of its impacts is the sense of bitterness inside the mouth. It implies in the reduction of appetite, which may finally result in the reduction of physical condition due to the inadequacy of food intake. It causes the inhibition of healing process, which reduces working productivity. The objective of this study was to identify the mechanism of bitterness due to inflammation, as proved locally in the taste buds of Wistar rats. This study was carried out experimentally using post-test only control design in experimental animals of male Wistar strain Rattus norvegicus. The animals were divided into two groups. First group served as control, while the second group received treatment with Salmonella typhimurium 0.5 ml/kg BW. Blood sample and tongue incision were taken from the animals. IL-1 was counted, and tongue incision was used for immunohistochemical staining for the variables of gustducin. Data were analyzed using Kolmogorov-Smirnov test for data normality, and followed with comparative test. The discriminant analysis was also done to find the discriminant variable. It was found that there was an increase of biological response of signaling transduction of bitterness in taste buds, as indicated from the increase of gustducin in treatment group or in inflammatory fever condition as compared to control group (p < 0.05), but no change of concertation at IL-1 significan whenever there was any change of concertation by unfolding its mechanism. Further studies can be recommended to find the way to inhibit this sense of bitterness. The results are intended to overcome homeostatic disorder in the body to prevent loss of appetite, so that physical endurance can be maintained. It concluded that there is no increase of serum IL-1 expression in fever, but there is a significanly increase of taste buds gustducin. Further studies should focus on gustducin cellular role in other factors that play a role in taste buds signal transduction, either in homeostatic condition or in the condition of homeostatic disorder.
The effect of Hegu acupoint stimulation in dental acupuncture analgesia Andrianto, Fransiskus; Sunariani, Jenny; Budhy S, Theresia Indah
Dental Journal (Majalah Kedokteran Gigi) Vol 40, No 1 (2007): (March 2007)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v40.i1.p37-41

Abstract

In daily life, dental treatments are often related with oral pain sensation which needs anesthesia procedures. Sometimes local anesthetics can not be used because patients have hypersensitive reaction or systemic diseases which may lead to complications. Stimulating acupoint, such as Hegu activates hypothalamus and pituitary gland to release endogenous opioid peptide substances that reduce pain sensitivity. The aim of the study was to determine Hegu acupoint stimulation effect on the pain sensitivity reduction in maxillary central incisor gingiva. The laboratory experimental research was conducted on 12 healthy male Wistar rats (3 months old, weights 150–200 grams). All rat samples received the same treatments and adapted within 1 month. The research was done in pre and post test control group design. 40-Volt electro-stimulation was done once on the maxillary central incisor gingiva prior to the bilateral Hegu acupoint stimulation, then followed by 3 times electro-stimulation with 3 minutes intervals. The pain scores were obtained based on the samples’ contraction in each electro-stimulation. The responses were categorized into 5 pain scores and statistically analyzed using Wilcoxon Test. The results showed that Hegu acupoint stimulation lowered the pain scores significantly (p < 0.05). Hegu acupoint stimulation could reduce the pain sensitivity in maxillary central incisor gingiva. Therefore, the use of acupuncture analgesia in dental pain management can be considered in the future.
THE EFFECTS OF NICOTINE ON THE PERIODONTAL TISSUE Herawati, Herawati; Sunariani, Jenny
Indonesian Journal of Tropical and Infectious Disease Vol 1, No 3 (2010)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.992 KB) | DOI: 10.20473/ijtid.v1i3.2199

Abstract

Tobacco contains thousands of chemical substances which known to be harmful to periodontal tissues. Nicotine was considered as the most toxic substances to periodontal tissues. The datas in this review indicate that smoking may have a significant role in the initiation and progression of periodontal destruction. The conclusion of this and the other studies indicate that smokers have a less favorable response to periodontal therapy than non smoker. Nicotine is potentially toxic substances that have a detrimental effect on periodontal tissue, by altering the host response or directly damage the cells of normal periodontium.
MECHANISMS OF PERIODONTITIS - INDUCED ATHEROSCLEROSIS Hudyono, Rikko; Sunariani, Jenny
Indonesian Journal of Tropical and Infectious Disease Vol 1, No 3 (2010)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.999 KB) | DOI: 10.20473/ijtid.v1i3.2197

Abstract

Nowadays CVD become the most common cause of death in US and worldwide. Atherosclerosis plays an important role in CVDs pathogenesis. Atherosclerosis decreases the elasticity of the vascular. Atherosclerosis shares the same risk factor as CVD, in which obesity, hyperlipidemia, hypertension and lack of physical activity may initiate it. However, 50% of all CVD patients are lack of the usual causes of CVD. The purpose of this review is to reveal the mechanism of periodontitis-induced atherosclerosis. Inflammation and autoimmune disease might play an important role in initiate the CVD. Periodontitis is one of the oral diseases which can cause systemic inflammation and may induce the atherosclerosis. Porphyromonas gingivalis (Pg) which is the major cause of periodontitis can induce it by expressing protein gp130 in its fimbriae. Periodontics patients are prone to have bacteremia by daily routine oral hygiene activity. Chronic bacteremia may alter the endothelial physiology, which is resulted in neointima formation, EC dysfunction, and lipid accumulation. It is concluded that periodontitis may play an important role in initiation and progression of atherosclerosis.
Changes of the sweet taste sensitivity due to aerobic physical exercise Wardhani, Ni Luh Putu Ayu; Irmawati, Anis; Sunariani, Jenny
Dental Journal (Majalah Kedokteran Gigi) Vol 44, No 1 (2011): (March 2011)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v44.i1.p35-38

Abstract

Background: Sweet taste is a pleasant sensation. Sweet taste is mostly consumed and fancied by many people. Physiologically, glucose is bodys source of energy, but if over used it can be affected to the bodys metabolism. This can be worsen if the persons not doing a healthy lifestyle. One way to implement a healthy lifestyle is by doing physical exercises. Purpose: The aim of this study was to determine changes in sensory sensitivity of sweet taste due to aerobic physical exercise. Methods: This study was conducted on subjects aged 20 to 30 years. The subjects did aerobic exercise using 80% load of MHR. The measurement sensitivity of the senses of the sweet taste was done for three times before the subject take aerobic physical exercise, four weeks after doing aerobic physical exercise, and eight weeks after doing aerobic physical exercise. Results: There was significant difference towards sensitivity of sweet taste sense before doing aerobic physical exercise, 4 week after doing the aerobic physical exercise, and 8 week after doing aerobic physical exercise. Conclusion: Aerobic physical exercise during eight weeks increase sweet taste sensitivity.Latar belakang: Rasa manis memberikan sensasi yang menyenangkan. Rasa manis merupakan jenis rasa yang paling banyak dikonsumsi dan disukai oleh sekelompok orang. Secara fisiologis, glukosa bisa berperan sebagai sumber energi, namun apabila dikonsumsi secara berlebihan dapat menimbulkan efek patologis. Hal ini dihubungkan dengan individu yang mempunyai gaya hidup yang tidak sehat. Salah satu cara yang bisa dilakukan untuk membiasakan gaya hidup sehat adalah dengan latihan fisik (olah raga). Tujuan: Penelitian ini bertujuan untuk membuktikan adanya perubahan sensitivitas indera kecap rasa manis setelah melakukan latihan fisik aerobik. Metode: Penelitian ini melibatkan subyek laki-laki, berusia 20–30 tahun. Subjek melakukan latihan fisik aerobik dengan intensitas sebesar 80% maximal heart rate. Sensitivitas indera kecap rasa manis diukur 3 kali, yaitu sebelum melakukan latihan fisik aerobik, dan 4 serta 8 minggu setelah latihan fisik aerobik. Hasil: Terdapat perbedaan yang signifikan pada sensitivitas indera kecap rasa manis sebelum 4 dan 8 minggu sesudah latihan fisik aerobik. Kesimpulan: Latihan fisik aerobik selama 8 minggu menyebabkan peningkatan sensitivitas indera kecap rasa manis.
The decrease of fibroblasts and fibroblast growth factor-2 expressions as a result of X-ray irradiation on the tooth extraction socket in Rattus novergicus Mahdani, Fatma Yasmin; Nirwana, Intan; Sunariani, Jenny
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i2.p94-99

Abstract

Background: Wound healing involves cellular, molecular, physiological, and biochemical processes as responses to tissue damage. For instance, when a failure during tooth extraction occurs, radiographic examination, X-rays, is required. X-rays as an enforcer diagnosis can damage DNA chain, resulting in cell death and inhibition of wound healing process. Purpose: This research aims to analyze fibroblasts cell number and fibroblast growth factor-2 (FGF-2) expressions during wound healing process after tooth extraction as a result of X-ray irradiation. Methods: There were three research groups, each consisting of ten rats. Incisor tooth extraction was performed on the left lower jaw, and then X-ray examination was conducted at certain irradiation doses, namely 0 mSv, 0.08 mSv, and 0.16 mSv. Those animals were sacrificed on day 3, and on day 7 after the extraction, histopathology and immunohistochemistry examinations were conducted to determine fibroblast cell number and FGF-2 expressions. Data obtained were then analyzed by oneway ANOVA and Tukey HSD tests. Results: The number of fibroblasts decreased significantly in the group with the irradiation dose of 0.16 mSv applied on day 7 after the extraction (p <0.05). Similarly, the number of FGF-2 expressions decreased significantly in the group with the irradiation dose of 0.16 mSv applied on days 3 and 7 after the extraction (p <0.05). Conclusion: X-ray irradiation at a dose of 0.16 mSv can inhibit the healing process of tooth extraction wound due to the decreasing of fibroblasts cell number and FGF-2 expressions.
The effect of capsicum frutescens-l to transient receptor potential vinaloid-1, toll like receptors (tlr-4) and interleukin 1 beta (il-1β) on periodontitis Sunariani, Jenny; Mooduto, Latief; Yuliati, Yuliati
Journal of Dentomaxillofacial Science Vol 2, No 2 (2017)
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v2i2.527

Abstract

Objective: Indonesia has many kinds of useful herbs, which are often used as species such as chili, pepper or cayenne pepper (Capsicum frutescens L). Previous study showed topical capsaicin can be used as a therapy to cure pain due to inflammation. Small concentrations of capsaicin can attenuate cytokines in the inflammatory process. Capsaicin studies in animal model showed activation of the transient receptor potential vanilloid-1. Capsaicin can decrease various cytokines such as IL-6, IL-12, IL-1β, and increase IL-10. Capsaicin is a natural agonist for transient receptor potential vanilloid-1. The aim of this study is to prove the effect of capsaicin on transient receptor potential vanilloid-1 expression and TNF-α and TLR-4 toward pain.Material and Methods: An experimental laboratory research used animal model Wistar male rats (Rattus novegicus) induced with Aggregatibacter actinomycetemcomitans serotype b and green chili extract (Capsicum frutescens L.) with a dose of 0.0912 mg/kg/day was applied to surface of the gingiva on maxillary first molar for 7 days. An immunohistochemical examination was conducted to see the density of transient receptor potential vanilloid-1, and the expression of TLR-4 and IL-1β in the mucosal tissues of the oral cavity.Results: There were significant differences in the applications of Capsicum frutescens L. with increasing of TRPV ligand-1 and IL-1β (p &lt; 0.05), while the TLR-4 (p &gt; 0.05) showed a significant difference to TRPV-1 and IL-1β. There is no significant difference to TLR-4.Conclusion: Capsaicin can increase TRPV-1 and decrease IL-1β but did not affect the TLR-4. Capsaicin can be used to decrease pain and accelerate healing process in periodontitis.