Intan Nirwana, Intan
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Kekuatan transversa resin akrilik hybrid setelah penambahan glass fiber dengan metode berbeda (The transverse strength of the hybrid acrylic resin after glass fiber reinforcement with different method) Nirwana, Intan
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 1 (2005): (March 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v38.i1.p16-19

Abstract

Different types of fibers have been added to acrylic resin materials to improve their mechanical properties. The purpose of this study was to know the transverse strength of the hybrid acrylic resins after glass fiber reinforcement with difference method. This study used rectangular specimens of 65 mm in length, 10 mm in width and 2.5 mm in thickness. There were 3 groups consisting of 6 specimens each, hybrid acrylic resin without glass fiber (control), glass fibers dipped in methyl methacrylate monomer for 15 minutes before being reinforced into hybrid acrylic resin (first method), glass fibers reinforced into a mixture of polymer powder and monomer liquid after the hybrid acrylic resin was mixed directly (second method). All of the specimens were cured for 20 minutes at 100° C. Transverse strength was measured using Autograph. The statistical analyses using one way ANOVA and LSD test showed that there were significant differences in transverse strength (p < 0.05) among the groups. The means of transverse strength were 94,94; 118,27; and 116,34 MPa. It meant that glass fibers reinforcement into hybrid acrylic resin enhanced their transverse strength compared with control. Glass fiber reinforcement into hybrid acrylic resin with differenciate method didn’t enhance their transverse strength.
Sitotoksisitas resin akrilik hybrid setelah penambahan glass fiber dengan metode berbeda (Cytotoxicity of the hybrid acrylic resin after glass fiber reinforcement with difference method) Nirwana, Intan; Soekartono, R. Helal
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 2 (2005): (June 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v38.i2.p56-59

Abstract

Glass fiber reinforcement of the hybrid acrylic resin with difference method can enhance residual monomer content of the material; it can cause cytotoxic effect on fibroblast cells. The purpose of this study was to know the cytotoxicity of hybrid acrylic resins after glass fiber reinforcement with difference method on the cultured fibroblasts. The squared specimens of 10 mm in length, 10 mm in width and 1.5 mm in thickness were cured for 20 minutes at 100° C. The fibroblast cells were grown in Eagles Minimum Essential Medium to be 2 × 105 cells/ml, then the cells were added to the samples in the plates and incubated at 37° C. After 48 hours, the cytotoxic effect was determined by direct cell number count using microscope and a hemocytometer. The statistical analyses using one way ANOVA and LSD test showed that there were significant difference in cell viability (p < 0.05) among the groups. The means percentage of cell viability were 90.00%, 99.,11%, 98.66%, it could be concluded that glass fiber reinforcement into hybrid acrylic resin with either first method or second method was not toxic.
FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract Aspriyanto, Didit; Nirwana, Intan; Budi, Hendrik Setia
Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 3 (2017): (September 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v50.i3.p121-126

Abstract

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.
The decrease of fibroblasts and fibroblast growth factor-2 expressions as a result of X-ray irradiation on the tooth extraction socket in Rattus novergicus Mahdani, Fatma Yasmin; Nirwana, Intan; Sunariani, Jenny
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i2.p94-99

Abstract

Background: Wound healing involves cellular, molecular, physiological, and biochemical processes as responses to tissue damage. For instance, when a failure during tooth extraction occurs, radiographic examination, X-rays, is required. X-rays as an enforcer diagnosis can damage DNA chain, resulting in cell death and inhibition of wound healing process. Purpose: This research aims to analyze fibroblasts cell number and fibroblast growth factor-2 (FGF-2) expressions during wound healing process after tooth extraction as a result of X-ray irradiation. Methods: There were three research groups, each consisting of ten rats. Incisor tooth extraction was performed on the left lower jaw, and then X-ray examination was conducted at certain irradiation doses, namely 0 mSv, 0.08 mSv, and 0.16 mSv. Those animals were sacrificed on day 3, and on day 7 after the extraction, histopathology and immunohistochemistry examinations were conducted to determine fibroblast cell number and FGF-2 expressions. Data obtained were then analyzed by oneway ANOVA and Tukey HSD tests. Results: The number of fibroblasts decreased significantly in the group with the irradiation dose of 0.16 mSv applied on day 7 after the extraction (p <0.05). Similarly, the number of FGF-2 expressions decreased significantly in the group with the irradiation dose of 0.16 mSv applied on days 3 and 7 after the extraction (p <0.05). Conclusion: X-ray irradiation at a dose of 0.16 mSv can inhibit the healing process of tooth extraction wound due to the decreasing of fibroblasts cell number and FGF-2 expressions.
Perlekatan koloni Streptococcus mutans pada permukaan resin komposit sinar tampak (The adherence of Streptococcus mutans colony to surface visible light composite resins) Anggraeni, Ajeng; Yuliati, Anita; Nirwana, Intan
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 1 (2005): (March 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v38.i1.p8-11

Abstract

Visible light composite resins was used to restore anterior and posterior teeth, and it is always covered by saliva pellicle. S. mutans can adhere to all of the surface of oral cavity and visible light composite resins. The aim of this study was to know the amount of S. mutans colony adherence to visible light composite resins surface. The sample of 5 mm diameter and 3 mm in thickness was immersed in saliva for one hour, than the samples were put into bacteria suspension, incubated for 24 hours at 37° C. The amount of S. mutans was determined by direct count using microscope. The data were statistically analyzed by using t test. The result showed a significance difference of S. mutans colony between hybrid and micro fill visible light composite resins. The conclusion was that the amount of S. mutans adherence on the surface of hybrid was higher than the micro fill visible light composite resins.
Application of pomegranate (Punica granatum Linn.) fruit extract for accelerating post-tooth extraction wound healing Nirwana, Intan
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 4 (2018): (December 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i4.p189-193

Abstract

Background: Trauma occurring during tooth extraction can cause complications such as bleeding, infection, fracture and dry socket and constitutes an inflammatory response trigger. Pomegranate (Punica granatum Linn.) extract, which contains large amounts of punicallagin and ellagic acid, possesses various qualities, including; anti-inflammatory, anti-bacterial and anti-oxidant. Pomegranate extract can inhibit proinflammatory cytokine production, while also suppressing inflammation response thereby accelerating wound healing. Purpose: This study aimed to analyze the effect of pomegranate extract application to the tooth extraction wounds of Cavia cobaya (C. cobaya) on the expression of fibroblast growth factor-2 (FGF-2) and transforming growth factor β (TGF-β) on the fourth day of the wound-healing process. Methods: This study used 12 C. cobaya, divided into two groups, namely; control and treatment. The subjects were anesthetized, before their lower left central incisor was extracted and the entire socket filled with CMC-Na 3% in members of the control group and pomegranate extract in those of the treatment group. The twelve C. cobaya were sacrificed on day 4, their lower jaw subsequently being removed and decalcified for approximately 30 days. The mandibula tissue was stained using a immunohistochemical technique. FGF-2 and TGF-β were used to evaluate the healing process in the extracted tooth socket. Differences in the expression of FGF-2 and TGF-β were evaluated statistically by means of a t-test. Results: This study indicated a significant difference between the control and the treatment groups (p<0.05). The treatment group members whose sockets were filled with pomegranate extract showed high FGF-2 and TGF-β expression. Conclusion: This study confirmed that the administration of pomegranate extract to post-extraction tooth wounds of C. cobaya increases the expression of FGF-2 and TGF-β on day 4, thereby accelerating the wound healing process.