Priyo Hadi, Priyo
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Fungal inhibitory effect of Citrus Limon peel essential oil on Candida albicans Hernawan, Iwan; Radithia, Desiana; Hadi, Priyo; Ernawati, Diah Savitri
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.332 KB) | DOI: 10.20473/j.djmkg.v48.i2.p84-88

Abstract

Background: Oral candidiasis is an opportunistic infections due to Candida albicans that often found in people with HIV/AIDS. Anti-fungi, polyne and azole, are used in the treatment of oral candidiasis, but often cause persistence and recurrence. Citrus Limon peel contains terpenoids capable of inhibiting the synthesis of ergosterol, a component of the fungal cell wall that helps to maintain cell membrane permeability. Essential oil derived from citrus limon peel, thus, is expected to inhibit the growth of Candida albicans. Purpose: This research was aimed to know how essential oil derived from citrus Limon peel can inhibit the growth of Candida albicans. Method: This research was a laboratory experimental research carried out in three phases. First, essential oil was made with cold pressing method, and then the concentration of 100% was diluted to 50%, 12.5%, 6.25%, 3.125%, 1.56% and 0.78%. A test was conducted on the culture of Candida albicans in Sabouraud broth, accompanied by control (+) and (-). Second, the dilution of essential oil was conducted to alter the concentration with inhibitory power, from the strongest one to the weakest one, and then it was tested on the culture of Candida albicans. Third, spreading was carried out from liquid culture to agar media in order to measure the number of colonies. Result: Candida albicans did not grow on media with 100% essential oil treatment, but it grew on media with 50% essential oil treatment. In the second phase, dilution of 100%, 90%, 80%, 70%, 60% and 50% was conducted. The growth of Candida albicans was found on the treatment media of 60% and 50%. On the agar media, the growth occurred in the cultured medium treated with 70%. Conclusion: The minimum inhibitory power of essential oil derived from citrus Limon peel against Candida albicans was in the concentration of 80%. Essential oil derived from citrus Limon peel has antifungal effect and potential as a therapeutic agent for oral candidiasis.
The effect of ethyl acetate fraction of Citrus limon peel on mesenchymal cell proliferation and polybacterial growth Marinna, Astrid; Hadi, Priyo; Radithia, Desiana
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 3 (2015): (September 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.407 KB) | DOI: 10.20473/j.djmkg.v48.i3.p144-149

Abstract

Background: Oral diseases remain to be global health problem. The common therapy involved the use of modern medicines with their various side effects. Citrus limon are potentials as anti-inflammatory, anti-fungal, anti-oxidant, anti-viral and anti-bacterial. Purpose: The purpose of this study was to determine the effect of ethyl acetate fraction of citrus limon peel extract on human gingival mesenchymal cell proliferation and palm commensal polybacterial growth. Method: This study was experimental study with post test only control group design. Citrus limon peel extracted and partitioned in order to obtain ethyl acetate fraction of 3.125%, 2.75%, 2.375%, 2%, and 1.5625%. Toxicity test was performed after 24 hours using the MTT Assays. Cell viability was measured by optical density formazonand read by ELISA reader 620 nm. Results: All treatment groups showed less than 60% cell viability. The highest cell was 19.36 (1.5625% concentration) and the lowest was 12.65 (3.125% concentration). The highest anti-bacterial inhibition value was 8.9125 mm (3.125% concentration) and the lowest was 6.0625 mm (1.5625% concentration). Conclusion: The higher concentration of ethyl acetate fraction Citrus limon peel extract, the higher toxicity and inhibitory properties against commensal palm polybacteria.
Tatalaksana ulkus yang disebabkan oleh pecahnya abses peritonsilar Management of ulcer due to rupture of peritonsillar abcess Palmasari, Astrid; Hadi, Priyo
Journal of Dentomaxillofacial Science Vol 10, No 3 (2011): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v10i3.278

Abstract

Peritonsillar abcess is a infection of head and neck commonly seen. This infection is caused by infection organismesuch as Streptococcus pyogenes (Group A Beta-Hemolitik streptococcus), Staphylococcus aureus, Haemophilusinfluenzae, Fusobacterium, Prevotella, Porphyromonas, and Peptostreptococcus spp. by the spreading of anaeroborganism to one of loose aerolar space around pharynx and cause the abcess and penentrate to the tonsillar capsulebut which is still in pharynx constrictor muscles boundary. This paper reports a male of 36 years old patient withchief complaint of ulcer on the hard palate accompanied with tonsillar swelling that made difficulty in swallowing.The diagnosis was ulceration due to peritonsillar abcess rupture. Amoxycyllin antibiotic, and benzydamine HClgargle was precribed to cure the ulcers.
Allergic contact cheilitis due to lipstick Ravitasari, Yatty; Radithia, Desiana; Hadi, Priyo
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 4 (2015): (December 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.075 KB) | DOI: 10.20473/j.djmkg.v48.i4.p173-176

Abstract

Background: Cheilitis is a common problem of unknown etiology. A possible cause of cheilitis is contact allergy. Drugs, lipsticks, sunblock and toothpaste are the most common implicated allergens. Allergic contact cheilitis is a chronic superficial inflammatory disorder of the vermilion borders characterized by desquamation due to delayed-type hypersensitivity reaction. Purpose: We report a management of Allergic contact cheilitis due to lipsticks. Case: A 21-year-old woman had a history of atopic allergy to eggs, milk, and chicken presented with sore, dry, fissured, scaled and sometimes bleeding lip, over a 3-month period after application of a lipstick. Her symptoms persisted despite treatments with hydrocortisone cream. The patient provided a detailed history and underwent physical examination and patch tests to cosmetic components and patch test to her own lipstick. The patient had strongly-positive result to the tested lipstick. A diagnosis of allergic contact cheilitis was made based on the history and clinical findings. Case management: Patient was advised to avoid wearing lipstick. To relieve symptoms, treatment was initiated with combined topical corticosteroid, antibiotic, and moisturizer. Conclusion: Contact allergy patients should be tested for both cosmetic component series and their own lipsticks to exclude exfolliative cheilitis, infection, or light actinic cheilitis as causal agents.
Acid fast bacilli detected in the oral swab sample of a pulmonary tuberculosis patient Bakti, Reiska Kumala; Mertaniasih, Ni Made; Ernawati, Diah Savitri; Soebadi, Bagus; Hadi, Priyo
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 2 (2018): (June 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i2.p91-94

Abstract

Background: Tuberculosis (TB) is an infectious disease that persists as a health problem worldwide. Mycobacterium tuberculosis, as an etiological agent, is transmitted from infected to uninfected individuals via airborne droplet nuclei. Oral health care workers or dental practitioners may be at high risk of TB infection because of their close proximity to infected individuals during treatment procedures. Simple and rapid screening of mycobacterium tuberculosis in the oral cavity is necessary in order to prevent transmission of infection. Purpose: To investigate the presence of acid-fast bacilli in the buccal mucosa of pulmonary TB patients. Methods: Nineteen pulmonary TB patients of both sexes, ranging in age from 19 to 74 years old participated in this study. The diagnosis of tuberculosis was performed by clinical symptom assessment and supporting examination, including acid-fast bacilli on sputum examination. Two buccal mucosa swabs taken from pulmonary TB patients were collected for acid fast bacilli direct smear by Ziehl Neelsen staining. Results: With regard to mycobacterium tuberculosis, acid-fast bacilli presented in 10.5% of the oral buccal mucosa swabs of subjects, whereas in the sputum specimens, bacilli were found in 52.6% of subjects. Conclusion: Acid-fast bacilli can be found in the buccal epithelial mucosa of pulmonary tuberculosis patients, although its presence was very limited.
PENGARUH PEMBERIAN ZnSO4 1 % TOPIKAL TERHADAP EKSPRESI MMP-9 DAN JUMLAH FIBROBLAS PADA PENYEMBUHAN ULKUS TRAUMATIKUS TIKUS WISTAR DIABETES MELITUS Hidayat, Lukman Hakim; Hadi, Priyo; Radithia, Desiana
E-Prodenta Journal of Dentistry Vol 2, No 2 (2018)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.718 KB) | DOI: 10.21776/ub.eprodenta.2018.002.02.4

Abstract

Diabetes mellitus adalah penyakit yang berdampak pada proses penyembuhan luka di dalam mulut. Pada penderita diabetes melitus, aktifitas prolifrasi fibroblast menurun dan aktifitas enzim kolagenese serta MMP-9 meningkat, sehingga penyembuhan ulserasi pada rongga mulut akan menjadi lebih lama. Zinc sulfat merupakan mineral yang dapat meningkatkan sinyal antar sel, proliferasi komponen penyembuhan, serta migrasi komponen pada tahapan penyembuhan.  Tujuan: untuk menganalisis pengaruh pemberian zinc sulfat 1% secara topikal pada ekspresi MMP-9 dan jumlah sel fibroblas jaringan mukosa mulut hewan coba pada kondisi diabetes mellitus, yang mengalami ulkus traumatikus. Metode: Pada penelitian ini hewan coba (48 ekor tikus wistar) berusia 2-3 bulan dan berat badan 150-200 gram, dibagi menjadi empat grup. Untuk mendapatkan tikus diabetes, maka subjek diinduksi dengan streptozotocin (30mg) yang dilarutkan dalam 1,33 ml buffer (asam sitrate). Untuk membuat ulser pada jaringan mukosa mulut, maka dilakukan dengan menempelkan burniser panas selama 10 detik pada mukosa bibir subjek. Pada setiap periode ekperimen (hari ke-5 dan ke-7) dilakukan pengambilan jaringan mukosa, dan dilanjutkan dengan pembuatan preparat histologi. Pada preparat histologi digunakan pengecatan HE untuk mengidentifikasi dan menghitung jumlah fibroblast, sedangakan IHS untuk mengidentifikasi dan menghitung ekspresi MMP-9. Metoda statistik one-way Anova digunakan untuk analisis perbandingan antara grup perlakuan dan grup kontrol. Signifikansi didasarkan pada nilai p<0.05. Hasil: Pemberian zinc sulfat 1% secara topikal pada ulkus traumtikus pada tikus diabetes mengakibatkan terjadi peningkatkan jumlah fibroblas (p<0,05) dan menurunkan ekspresi MMP-9 (p<0,05) yang diamati pada setiap periode eksperimen yang ditetapkan dalam penelitian ini. Kesimpulan: Zinc sulfat 1% dapat mempercepat proses penyembuhan ulkus traumatikus pada hewan coba dengan kondisi diabetes mellitus. Kata kunci : diabetes melitus, ulkus traumatikus, zinc sulfat , MMP-9, fibroblas.