Annisa Trissatharra, Annisa
Unknown Affiliation

Published : 2 Documents
Articles

Found 2 Documents
Search

Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus) Trissatharra, Annisa; Dwiningsih, Sri Ratna; Munir, Ratna Sofaria
Majalah Obstetri & Ginekologi Vol 24, No 1 (2016): Januari - April 2016
Publisher : Majalah Obstetri & Ginekologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.595 KB) | DOI: 10.20473/mog.v24i1.2764

Abstract

Tujuan: Untuk mengetahui pengaruh antibodi monoklonal bZP3 dalam folikel ovarium yang mengalami atresia dan diameter berbagai folikel ovarium.Bahan dan Metode: Penelitian ini merupakan penelitian eksperimental sejati dengan post only control group design. Sampel berupa 36 mencit (Mus musculus) betina yang terbagi menjadi 6 kelompok, 3 kelompok kontrol (kelompok 1, 2, dan 3) diberi Phospatase Buffer Saline (PBS) 50 µl dan 3 kelompok perlakuan (kelompok 4, 5, dan 6) diberi Mab bZP3 50 µl. Kelompok 1 dan 4 dihentikan pada hari ke-5, kelompok 2 dan 5 dihentikan pada hari ke-10, dan kelompok 3 dan 6 dihentikan pada hari ke-20. Evaluasi folikel atresia ovarium dan diameter folikel ovarium dilakukan dengan hematoxylin eosin (HE) dan data diolah dengan statistik parametrik.Hasil: Tidak ada perbedaan signifikan pada berbagai aspek folikel atresia dan diameter folikel (p> 0,05), namun secara deskriptif, jumlah folikel yang mengalami atresia pada kelompok perlakuan primer, sekunder, dan tersier lebih tinggi daripada kelompok kontrol, kecuali pada hari ke-20 waktu observasi.Simpulan: Pemberian Mab bZP3 tidak berpengaruh terhadap jumlah folikel atresia dan diameter folikel selama waktu pengamatan.
Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus) Trissatharra, Annisa; Dwiningsih, Sri Ratna; Munir, Ratna Sofaria
Majalah Obstetri & Ginekologi Vol 24, No 1 (2016): Januari - April
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.533 KB) | DOI: 10.20473/mog.V24I12016.37-42

Abstract

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.