Eha Renwi Astuti, Eha Renwi
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The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture Mulyani, Sri Wigati Mardi; Setiawati, Ernie Maduratna; Safitri, Erma; Astuti, Eha Renwi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p41-44

Abstract

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.Latar belakang: Konsep terapi stem cell merupakan salah satu harapan baru sebagai terapi medis kelainan kelenjar ludah. Namun, rendahnya viabilitas stem cell yang ditransplantasikan menyebabkan penurunan efektivitas terapi. Asumsi yang mendasari rendahnya viabilitas dan fungsi stem cell adalah tingginya kejadian apoptosis. Hal ini menunjukkan bahwa lingkungan mikro di daerah jaringan yang rusak tidak kondusif untuk mendukung viabilitas stem cell. Salah satu lingkungan mikro adalah kondisi hipoksia. Beberapa jurnal ilmiah mengungkapkan bahwa kondisi hipoksia pada kultur sel dapat menyebabkan sel-sel stres, namun di sisi lain kondisi stres sel juga merangsang heat shock protein 27 (HSP 27) sebagai antiapoptosis dengan menghambat ekspresi caspase 9. Tujuan: Tujuan penelitian ini adalah untuk meneliti peran protein heat shock 27 sebagai inhibitor apoptosis dalam kondisi hipoksia kultur stem cell sumsum tulang. Metode: Kultur stem sel dilakukan dalam kondisi hipoksia (O2 1%) dan mengukur resistensi terhadap apoptosis melalui ekspresi HSP 27 dan caspase 9 stem cell mesenchymal sumsum tulang dengan menggunakan immunoflorecence dan PCR real time. Hasil: Hasil penelitian menunjukkan bahwa prakondisi hipoksia dapat menghambat apoptosis melalui peningkatan HSP 27 dan penurunan tingkat Caspase 9. Simpulan: Studi ini menunjukkan bahwa prakondisi hipoksia dapat mengurangi apoptosis dengan meningkatkan jumlah protein heat shock 27 dan penurunan caspase 9.
VEGF expression and new blood vessel after dental X-ray irradiation on fractured tooth extraction wound Woroprobosari, Niluh Ringga; Sunariani, Jenny; Astuti, Eha Renwi
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 3 (2015): (September 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i3.p159-164

Abstract

Background:Dental X-ray has an important role in dentistry. Complication case such as tooth fracture extraction requires this examination to determine the appropriate treatment measures. Dental X-ray can also cause a negative impact to the body at cellular and even molecular level. Purpose: The aim of this study was to evaluate the decrease of vascular endothelial growth factor (VEGF) expression and new blood vessels number caused by dental X-ray irradiation on fractured tooth extraction wound on day 3 and 7 after extraction. Method: We used 30 wistar rats which was randomly divided into 6 groups. Each rat’s central insisive of left mandible was fractured and then extracted after or without X-ray irradiation. Group KA and KB were control groups without irradiation. Group P1 A and P1 B were treatment groups with 0.08 mSv irradiation dose. Group P2 A and P2 B were treatment groups with 0.16 mSv irradiation dose. The subject from group KA, P1 A, and P2 A were sacrficed and sockets were collected at day 3. The subject from group KB, P1 B, and P2 B were sacrficed and sockets were collected at day 7. Socket were processed and painted with hematoxylin eosin and immunohistochemistry, then observed with a microscope. Data processing was performed with SPSS 16 through one way anova test and post hoc Tukey test HS. Result: The lowest means expression of VEGF and the number of new blood vessels on the day 3 was found in P2 A group, and the highest found in the KA group. The lowest means expression of VEGF and the number of new blood vessels on the day 7 was found in P2 B group, and the highest found in the KB group. Conclusion: Dental X-ray irradiation dose of 0.08 mSv and 0.16 mSv causes decrease of VEGF expression and new blood vessels in the wound fractured tooth extraction in day 3 and day 7 post-extraction.
Transforming growth factor beta 1 expression and inflammatory cells in tooth extraction socket after X-ray irradiation Putra, Ramadhan Hardani; Astuti, Eha Renwi; Ridwan, Rini Devijanti
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 2 (2016): (June 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v49.i2.p87-92

Abstract

Background: Radiographic examination is often used in dentistry to evaluate tooth extraction complications. X-ray used in radiographic examination, however, has negative effects, including damage to DNA and inflammatory response during wound healing process. Purpose: This study aimed to analyze the effects of X-ray irradiation on transforming growth factor beta 1 (TGF-ß1) expression and number of inflammatory cells in tooth extraction sockets. Method: Thirty rats were divided into three groups, which consist of control group (with a radiation of 0 mSv), treatment group 1 (with a radiation of 0.08 mSv), and treatment group 2 (with a radiation of 0.16 mSv). These rats in each group were sacrificed on days 3 and 5 after treatment. Inflammatory cells which were observed in this research were PMN, macrophages, and lymphocytes. Histopathological and immunohistochemical examinations were used to calculate the number of inflammatory cells and TGF-ß1 expression. Obtained data were analyzed using SPSS 16.0 software with one way ANOVA and Tukey’s HSD tests. Result: There was no significant decrease in the number of PMN. On the other hand, there were significant decreases in the number of macrophages and lymphocytes in the sacrificed group on day-5 with the radiation of 0.16 mSv. Similarly, the most significant decreased expression of TGF-ß1 was found in the group sacrificed on day 5 with the radiation of 0.16 mSv. Conclusion: X-ray irradiation with 0.08 mSv and 0.16 mSv doses can decrease TGF-ß1 expression and number of inflammatory cells in tooth extraction sockets on day 3 and 5 post extraction.
AKTIVITAS SUPEROKSIDA DISMUTASE, KATALASE DAN KADAR MALONDIALDEHIDA KELENJAR SUBMANDIBULARIS TIKUS WISTAR SETELAH IRADIASI SINAR GAMMA Hayati, Kemala; Astuti, Eha Renwi; Martini, Tri
250-20412
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstractA common side effect of radiotherapy used in the treatment of head and neck cancers is the occurrence of structural and physiological alteration of the salivary gland due to exposure to ionizing irradiation, as demonstrated by conditions such as decreased salivary flow. Ionizing irradiation cause burst of reactive oxygen species (ROS) such as superoxide, hydroxil and hidrogen peroxide which induce activation of self-defense system such as superoxide dismutase and catalase. If this defense system could not diminish the excessive amount of  ROS it would lead to oxidative stress which can be determined by rise of malondialdehide (MDA) levels. The aim of this research is to find out the influence of single and fractionated dose of gamma ray irradiation on superoxide dismutase and catalase activities and malondialdehide levels in rat submandibular glands at 24 hours, 3 weeks and 6 weeks after exposure of gamma ray irradiation. For this research, experimental laboratory was done. Sixty male Wistar rats aged 3-4 months (250-300 g) grouped into three. Group A (20 rats) as control group were not irradiated. Group B (20 rats) were irradiated with single dose (10 Gy) and group C were irradiated with fractionated dose (10 Gy in 5 fraction of 2 Gy/day) of Co60 Gamma ray, with their neck ventral surface exposed to the source. The rat submandibular glands were extirpated at 24 hours, 3 weeks and 6 weeks after irradiation and then analysed for superoxide dismutase and catalase activities using microreader and malondialdehide levels using spectrophotometer. There were significant differences (p:0,000) of superoxide dismutase and catalase activities and malondialdehide levels after gamma ray irradiation at evaluation time 24 hours, 3 weeks and 6 weeks. Superoxide dismutase activity in group B (10 Gy single dose irradiation) and group C (10 Gy fractionated dose irradiation) decreased in compare to group A (control). Catalase activity in group B decreased in compare to group A at 24 hours, in group C catalase activity increased in compare to group A. Malondialdehide levels increased in group B and C compare to group A at 24 hours, 3 weeks and 6 weeks after irradiation.
Changes in the number of macrophage and lymphocyte cells in chronic periodontitis due to dental X-ray exposure Asymal, Alhidayati; Astuti, Eha Renwi; Devijanti, Rini
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 2 (2018): (June 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i2.p99-103

Abstract

Background: Periodontitis is an inflammatory disease caused by specific microorganisms that attacks tooth-supporting tissues, P. gingivalis bacteria are mostly found in patients suffering from chronic periodontitis which is usually diagnosed by means of clinical and radiographic examination. The latter play important roles in the management of periodontitis, including: establishing diagnosis, determining treatment plans and evaluating the results of treatment. Unfortunately, the use of X-rays to perform such radiographic examination has negative effects since the body’s various parts, especially the head, are not well protected from the effects of X-ray radiation. Purpose: This research aimed to analyze the effects of dental X-ray exposure on the number of macrophages and lymphocytes in experimental subjects suffering from periodontitis. Methods: 36 rats that had been diagnosed with chronic periodontitis were divided into three groups, namely: a control group, treatment group I (exposed to a 0.16 mSv dose of radiation) and treatment group II (exposed to a 0.32 mSv dose of radiation). These subjects were subsequently sacrificed on the third and fifth days after treatment. Thereafter, histopathological examination was performed to identify any changes in the number of macrophages and lymphocytes. Results: The results of an HSD test confirmed that, on the third day, there were significant differences in the number of lymphocytes between the control group and treatment group I, as well as between the control group and treatment group II. On the fifth day, there were also significant differences in the number of lymphocytes between the control group and treatment group I, as well as between treatment group I and treatment group II. Similarly, there was a significant difference in the number of macrophage cells on the third day between the control group and treatment group I. On the fifth day, there were also significant differences in the number of macrophage cells between the control group and treatment group I, as well as between treatment group I and treatment group II. Conclusion: Dental x-ray exposure at a dose of 0.16 mSv can elevate the number of macrophages and lymphocytes on the third and fifth days. On the other hand, dental x-ray radiation at a dose of 0.32 mSv can reduce the number of macrophages on day 3 as well as the number of lymphocytes on the third and fifth days.