Basri A. Gani, Basri A.
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The pH changes of artificial saliva after interaction with oral of artificial saliva after interaction with oral micropathogen Gani, Basri A.; Soraya, Cut; Sunnati, Sunnati; Nasution, Abdillah Imron; Zikri, Nurfal; Rahadianur, Rina
Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 4 (2012): (December 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v45.i4.p234-238

Abstract

Backgorund: Saliva contains several protein elements, exocrine proteins and antibodies, such as lactoferrin, sIgA, peroxidase, albumin, polypeptides, and oligopeptides that contribute to the defense of oral mucosa and dental pellicle to prevent infection caused by oral micropathogen, such as Candida albicans, Streptococcus mutans and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Those micropathogens have a role to change salivary pH as an indicator of oral disease activities. Purpose: This study was aimed to analyze the changes of artificial saliva pH after interaction with S. mutans, C. albicans, and A. Actinomycetemcpmitans. Methods: The materials used in this study consist of S. mutans (ATCC 31987), C. albicans (ATCC 10231), A. actinomycetemcomitans (ATTC 702 358), and artificial saliva. To examine the pH changes of artificial saliva, those three microbiotas were cultured and incubated for 24 hours. Results: The results showed that the interactions of S. mutans, C. albicans, and A. actinomycetemcomitans in the artificial saliva can change the salivary on neutral. There were no significant difference with the control treatment salivary pH 4, 5, 6, 8, and 9 (p>0.05). Similarly, there was also no significant difference when those three microorganism interacted each other in the artificial saliva (p<0.05). Conclusion: It can be concluded that the biological activity of S. mutans, C. albicans, and A. actinomycetemcomitans in artificial saliva can change the salivary pH into neutral. It indicates that those microbiotas mutually supported and cooperated in influencing the biological cycle of the oral cavity with salivary pH as an indicator.Latar belakang: Saliva merupakan cairan eksokrin yang mengandung unsur protein dan antibodi seperti sIgA laktoferin peroksidase, albumin, polipeptida dan oligopeptida yang berperan pada pertahanan mukosa rongga mulut dan gigi guna mencegah infeksi oral mikropatogen seperti C. albicans, S. mutans, dan A. actinomycetemcpmitans. Patogenesis ketiga oral mikropatogen tersebut diawali dengan mempengaruhi perubahan pH saliva sebagai langkah invasi dan infeksi pada mukosa oral dan pelikel gigi. Tujuan: Penelitian ini bertujuan untuk untuk mengetahui perubahan pH saliva buatan setelah diinteraksikan dengan S. mutans, C. albicans, dan A. Actinomycetemcpmitans. Metode: Materi penelitian ini berupa Streptococcus mutans strain ATCC 31987, Candida albicans strain ATCC 10231, Aggregatibacter actinomycetemcomitans strain ATTC 702358, dan saliva buatan. Untuk mengetahui perubahan pH saliva, maka ketiga mikrobiota tersebut dikultur dan untuk menguji perubahan pH saliva dilakukan uji interaksi ketiga mikroorganisme tersebut dalam saliva buatan selama 24 jam dengan pengaturan pH saliva sebagai indikator hasil penelitian. Hasil: Hasil penelitian menunjukkan interaksi S. mutans, C. albicans, dan A. actinomycetemcomitans dalam saliva buatan mampu mereduksi perubahan pH saliva mengarah ke pH netral dengan kontrol perlakuan pH saliva 4, 5, 6, 8, dan pH 9 secara statistik tidak tidak menunjukkan perbedaan bermakna (p>0,05), begitu juga ketika dilakukan interakasi diantara masing-masing mikroorganisme tersebut dalam saliva buatan menunjukkan adanya perbedaan bermakna (p<0,05). Kesimpulan: Dapat disimpulkan bahwa aktivitas biologi S. mutans, C. albicans, dan A. actinomycetemcomitans dalam saliva buatan mampu merubah pH Saliva sekaligus mempertahankan pH netral. Hal ini menggambarkan bahwa mikrobiota tersebut saling mendukung dan bekerjasama dalam mempengaruhi siklus biologi rongga mulut dengan pH saliva sebagai indikator.
Potential of Jatropha multifida sap against traumatic ulcer Gani, Basri A.; Nasution, Abdillah Imron; Nazaruddin, Nazaruddin; Sartika, Lidya; Alam, Rahmat Kurniawan
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 3 (2015): (September 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i3.p119-125

Abstract

Background: Traumatic ulcer is a lesion in oral mucosa as a result of physical and mechanical trauma, as well as changes in salivary pH. Jatropha multifida sap can act as antimicrobial, anti-inflammatory and re-epithelialization, and can also trigger the healing process of ulcers. Purpose: Research was aimed to determine the potential of Jatropha multifida sap against traumatic ulcer base on clinical and histopathological healing process. Method: This research was conducted laboratory experimental model, with rats (Rattus norvegicus) as the subject as well as Jatropha multifida sap for ulcer healing. Those subjects were divided into four groups: two treatment groups administrated with pellet and Jatropha multifida sap, one group as the positive control group administrated with 0.1% triamcinolone acetonide, and one group as the negative control group administrated with 0.9% NaCl. Ulcer manipulation was used 30% H2O2, and evaluation of ulcer healing was used clinical and histopathological approach. Result: Clinically, the healing process of ulcers in the treatment group with Jatropha multifida sap was faster than that in the positive control group with 0.1% triamcinolone acetonide, indicated with the reduction of the ulcer size until the missing of the ulcers started from the third day to the seventh one (p≤0.05). Histopathologically inflammatory cells (lymphocytes, and plasma cells) declined started from the third day, and the formation of collagen and re-epithelialization then occurred. On the seventh day, the epithelial cells thickened, and the inflammatory cells infiltrated. Statistically, those groups were significant (p≤0.05). Conclusion: Jatropha multifida sap has a significant potential to cure traumatic ulcers on oral mucosa clinically and histopathologically.
The ability of IgY to recognize surface proteins of Streptococcus mutans Gani, Basri A.; Chismirina, Santi; Hayati, Zinatul; B, Endang Winiati; Bachtiar, Boy M.; Wibawan, I. Wayan T.
Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 4 (2009): (December 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v42.i4.p189-193

Abstract

Background: Streptococcus mutans are gram positive bacteria classified into viridians group, and have a role in pathogenesis of dental caries. It’s adhesion to the tooth surface is mediated by cell surface proteins, which interact with specific receptor located in tooth pellicle. Glucan binding protein, Glukosyltransferase, and antigen I/II are basic proteins of S. mutans, which have a role in initiating the interaction. A previous study showed that chicken’s IgY can interfere the interaction. Purpose: The objective of this study was to assess the ability of IgY in recognizing the surface molecule of Streptococcus mutans expressed by various serotypes (c, d, e, f) and a strain derived from IPB, Bogor. Method: Western blot was used as a method to determine such capability. Result: The result showed that IgY has a potency to recognize antigen I/II, but not the other proteins on the cell surface of all bacteria tested. Conclusion: The ability of IgY to bind the surface protein, antigen I/II, indicates that this avian antibody could be used as a candidate for anti-adhesion in preventing dental caries.
Immuno-biokompatibilitas Pada Material Implan: Review Article Gani, Basri A.
Cakradonya Dental Journal Vol 7, No 2 (2015): Desember 2015
Publisher : FKG Unsyiah

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Abstract

Imuno-biokompatibilitas merupakan pengetahuan yang secara khusus mempelajari respons imun terhadap biomaterial, prosthesa, alat kedokteran memiliki hubungan yang erat dengan kemampuan material implan beradaptasi dengan perubahan fisiologi tubuh seperti interaksi komponen darah, akumulasi partikel, dan respons terhadap organ. Tulisan ini menjelaskan beberapa variabel yang berhubungan dengan biokompatibilitas seperti komponen biokompatibilitas, biokompatibilitas pada dental material, imunokompatibilitas, serta pertimbangan uji biokompatibilitas yang direferensikan oleh badan standar internasional seperti FDA, NAMSA dan standar ISO 10993. Aspek yang menjadi penilaian penting dari biokompatibilitas adalah kesesuaian antara perubahan sistem imun dengan adaptasi material implan yang tidak merugikan host seperti kerusakan sel dan jaringan sebagai akibat dari toksisitas, imunogenesitas, genetoksisitas, dan mutagenitas.
TOPOGRAFI DENTIN SETELAH PENYIKATAN DENGAN SODIUM LAURYL SULFATE PADA BERBAGAI DURASI WAKTU DITINJAU DENGAN ATOMIC FORCE MICROSCOPY Nasution, Abdillah Imron; Gani, Basri A.; Asbarini, Firda
Cakradonya Dental Journal Vol 10, No 1 (2018): Februari 2018
Publisher : FKG Unsyiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v10i1.10613

Abstract

Menyikat gigi menggunakan pasta gigi berfluoride adalah kebiasaan yang sering dilakukan masyarakat di negara berkembang. Pasta gigi yang dijual di pasaran biasanya mengandung deterjen dengan kadar yang rendah. Sodium lauryl sulfate (SLS) adalah salah satu deterjen dalam pasta gigi dengan kadar rata-rata 0,5-2% dari berat keseluruhan pasta gigi. SLS dapat merusak struktur dentin dengan berpenetrasi ke dalam kristal hidroksiapatit (HA) yang merupakan penyusun dentin. Penelitian ini bertujuan menganalisis topografi dentin setelah penyikatan dengan sodium lauryl sulfate 1% pada berbagai durasi waktu ditinjau dengan Atomic Force Microscopy (AFM). Enam gigi premolar digunakan sebagai spesimen dan dipotong pada area mahkota dekat CEJ kemudian dihaluskan. Spesimen dikelompokkan ke dalam enam kelompok yaitu kelompok kontrol negatif, kontrol positif, dan kelompok yang disikat dengan SLS 1% dengan durasi 3 menit, 5 menit, 8 menit dan 10 menit. Perlakuan diulang selama 7 hari. Hasil pengamatan AFM memperlihatkan perbedaan antara kelompok kontrol dengan kelompok perlakuan. Hasil penelitian menunjukkan bahwa penyikatan menggunakan SLS dapat menurunkan kekasaran permukaan, memperkecil diameter tubulus dentin, menurunkan tinggi dentin intertubuler dan memperlebar jarak dentin intertubuler. Oleh karena itu, dapat disimpulkan bahwa SLS dapat menyebabkan abrasi pada struktur hidroksiapatit dan merusak kolagen pada dentin.
Tingkat Sensitivitas Dentin Sebelum dan Setelah Paparan Minuman Bersoda Pada Usia Remaja Berdasarkan Metode Visual Analog Scale Chismirina, Santi; Gani, Basri A.; Harahap, Mizwan Fachry
Cakradonya Dental Journal Vol 7, No 1 (2015): Juni 2015
Publisher : FKG Unsyiah

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Abstract

Hipersensitivitas dentin terjadi karena terpaparnya dentin akibat terkikisnya email yang disebabkan proses demineralisasi email. Minuman bersoda merupakan salah satu minuman yang dapat menyebabkan demineralisasi email. Remaja merupakan salah satu pengkonsumsi minuman bersoda terbanyak. Oleh karena itu, penelitian ini bertujuan untuk mengetahui tingkat sensitivitas dentin sebelum dan setelah paparan minuman bersoda pada usia remaja berdasarkan metode Visual Analog Scale (VAS). Metode yang digunakan adalah Garis Linier dan Faces Pain Scales. Tahap pertama dari penelitian ini dimulai dengan seleksi subjek yang dilanjutkan pemeriksaan hipersensitivitas dentin sebelum paparan minuman bersoda dan pemeriksaan sensitivitas dentin setelah paparan minuman bersoda. Dari hasil pemeriksaan 39 subjek penelitian, 25 subjek menderita sensitif ringan sebelumdipaparkan minuman bersoda dan 23 subjek penelitian menderita sensitif sedang setelah dipaparkan minuman. Dari hasil uji statistik menunjukan perbedaan tingkat sensitivitas dentin yang signifikan sebelum dan setelah paparan minuman bersoda. Berdasarkan hasil penelitian dapat disimpulkan bahwa paparan minuman bersoda dapat mempengaruhi tingkat sensitivitas dentin.
The correlation between pH and flow rate of salivary smokers related to nicotine levels labelled on cigarettes Saputri, Dewi; Nasution, Abdillah Imron; Surbakti, Mutiara Rizki Wardarni; Gani, Basri A.
Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 2 (2017): (June 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v50.i2.p61-65

Abstract

Background: Saliva is a biological fluid in oral cavity that plays a role in maintaining the environmental balance and oral commensal. Nicotine of cigarettes has been reported as a predisposing factor for changing of pH and salivary flow rate, thereby changing in biological salivary components. Purpose: This study aimed to analyze the correlation between salivary pH and salivary flow rate in smokers with nicotine levels labeled on cigarettes. Methods: Purposive sampling was conducted involving 40 male smokers. Before participating, they filled a questionnaire related to the history of their smoking habit. Using a spitting method for 5 minutes their saliva was collected. Results: Result of Pearson correlation test showed that there was a significant correlation between smoking intensity and salivary flow rate of those smokers (r = -0.486 and p<0.001). The results also indicated that there was a significant correlation between smoking intensity and salivary pH (r = -0.376 and p<0.017). On the other hand, there was no significant correlation between nicotine levels levels labeled on cigarettes with salivary pH of those smokers (r = -0.107, p>0.512). There was no correlation between nicotine levels labeled on cigarettes and salivary pH of those smokers (r = -0.216, p>0.181). Nevertheless, there was a significant correlation between salivary flow rate and salivary pH of those smokers (r= 0.686, p<0.00,). Conclusion: There is a strong correlation between the intensity of smoking with salivary flow rate and its pH. However, there is no correlation between nicotine levels labeled on cigarettes and both salivary flow rate as well as salivary pH.
PROFILE OF ALLERGY HYPERPLASMA PATHOLOGIC ANTIBODY AND IMMUNOGENIC CHARACTERISTIC Nazaruddin, Nazaruddin; Budiman, Hamdani; Gani, Basri A.; Jakfar, Subhaini; Hasan, M.; Hanafiah, Muhammad
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5299

Abstract

The objective of this research was to determine the allergy hyperplasma pathologic antibody and to evaluate the immunogenic characteristics. Blood and serum were collected from human suffering rhinitis, skin, eye and asthma allergies. To obtain a cloned allergen plasma protein (protein allergen that had been induced with the serum from human with allergic rhinitis, skin, eye and asthma), the goat was used as an intermediate animal. Hematological analysis showed that the leukocyte cell such as neutrophil, lymphocyte, monocyte, and eosinophil increase in allergic-suffered human. The blood smear test exhibited that the mastocyte cell was dominant which contributes to allergy activities in human body. The sodium dodecyl sulphate-polyacrylimide gel electrophoresis (SDS-PAGE) assay detected protein allergens with molecular weight of 188 kDa (IgE) and 60-62 kDa (mastocyte cell). The reactivity assay using enzyme linked immunosorbent-assay (ELISA) revealed that cloned-allergens (whole hyperplasma allergen from goat isolates) express the best reactivity at various concentrations of IgE than the leukocyte cells. This research concluded that the clones of protein allergen have better immunogenic characteristic and those proteins can be recommended as the candidate of allergen to induce the humoral immunity on host and deliver specific product of anti-allergy such as milk.