Pratiwi Soesilawati, Pratiwi
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Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient Wati, Sisca Meida; Istiati, Istiati; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p141-145

Abstract

Background: Human periodontal diseases are inflammatory disorders as the result of complex interactions between periodontopathogens and the host’s immune response. Periodontitis results in tooth loss and can even lead to systemic diseases if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamated sites. Polymorphonuclear leukocyte (PMNs) infiltration can be seen in each stage of periodontitis. Lactoferrin is one of the PMN specific granules and could be a useful marker of PMN activity. Purpose: The aim of this study was to determine the band intensity of lactoferrin used as periodontitis biomarker. Methods: Gingival crevicular fluid (GCF) samples were collected using paper point no.30 from 40 subjects, 30 periodontitis patients that devide according to the severity (10 mild periodontitis, 10 moderate periodontitis, and 10 severe periodontitis) and 10 healthy controls, ranging in ages from 20 to 35 years. GCF lactoferrin was analyzed by Western blot and measured the band intensity by quantity one software (bio-rad). Results: The periodontitis sites exhibited significantly greater band intensity of lactoferrin than healthy sites. The band intensity of lactoferrin was positively correlated with the severity of periodontitis (α = 0.05). Conclusion: The study showed that the intensity of the lactoferrin protein bands could be used as biomarkers of periodontitis.Latar belakang: Penyakit periodontal adalah gangguan inflamasi yang merupakan hasil dari interaksi yang kompleks antara periodontopathogens dan respon imun host. Periodontitis mengakibatkan hilangnya gigi dan bahkan dapat menyebabkan penyakit sistemik jika tidak diobati. Cairan sulkus gingiva (GCF) mencerminkan kondisi gingiva dan mengandung protein yang tertransudasi dari serum atau sel pada lokasi radang. Infiltrasi polymorphonuclear leukosit (PMN) dapat dilihat pada setiap tahap periodontitis. Laktoferin adalah salah satu granula spesifik PMN dan bisa menjadi indicator aktivitas PMN. Tujuan: Tujuan penelitian ini adalah untuk meneliti intensitas band laktoferin dapat sebagai biomarker periodontitis. Metode: Cairan sulkus gingiva (GCF) dari tiap sampel dikumpulkan menggunakan paper pint no. 30 dari 40 subjek, 30 pasien dengan periodontitis yang dibagi sesuai dengan tingkat keparahan (10 periodontitis ringan, 10 periodontitis moderat, dan 10 periodontitis parah) dan 10 kontrol, mulai usia 20-35 tahun. GCF lactoferrin dianalisis dengan Western blot dan diukur intensitas bandnya dengan quantity one software (bio-rad). Hasil: Pada jaringan yang mengalami periodontitis menunjukkan intensitas band yang secara signifikan lebih besar dari laktoferin daripada periodontal yang sehat. Intensitas band laktoferin berkorelasi positif dengan tingkat keparahan periodontitis (α = 0,05) Simpulan: Hasil penelitian ini menyimpulkan bahwa intensitas band protein laktoferin dapat digunakan sebagai biomarker periodontitis.
The effects of golden sea cucumber extract (Stichopus hermanii) on the number of lymphocytes during the healing process of traumatic ulcer on wistar rat’s oral mucous Arundina, Ira; Yuliati, Yuliati; Soesilawati, Pratiwi; Damaiyanti, Dian W; Maharani, Dania
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i2.p100-103

Abstract

Background: Indonesia is a country with the world’s biggest potential and producer of sea cucumbers. Golden sea cucumber contains glicosaminoglycans, such as heparan sulphate and chondroitin sulphate, which could have a positive implication on wound healing process. This acceleration of wound healing process could be observed through the increasing of lymphocytes on ulcus traumaticus. Purpose: This study aims to analyze the effects of golden sea cucumber extract on the number of lymphocytes during the healing process of traumatic ulcer on Wistar rat’s oral mucous. Method: Golden sea cucumber extrat was made with freeze-dried method, and then gel was prepared using PEG 400 and PEG 4000 solvent. Twenty male rats with mucosal ulcus made were divided into a control group and three treatment groups with 20%, 40% and 80% golden sea cucumber extracts. All samples were euthanized on day 4 and then a preparation for histopathological examination was made to examine the number of lymphocytes. Result: The biggest number of lymphocytes was found in the treatment group with 40% golden sea cucumber extract, while the lowest one was found in the control group. The results of one way Anova test then showed a significant difference between the control group and the treatment groups. And, the results of Tukey HSD showed a significant difference between the control group and the treatment group with 40% golden sea cucumber extract. Conclusion: It can be concluded that 40% golden sea cucumber (Stichopus hermanii) extract can increase the number of lymphocytes during the healing process of traumatic ulcer on Wistar rat’s oral mucous.
Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats Oentaryo, Gunawan; Istiati, Istiati; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 3 (2016): (September 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v49.i3.p125-132

Abstract

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.
Cytotoxicity test of 40, 50 and 60% citric acid as dentin conditioner by using MTT assay on culture cell line Khoswanto, Christian; Arijani, Ester; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 3 (2008): (September 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v41.i3.p103-106

Abstract

Background: Open dentin is always covered by smear layer, therefore before restoration is performed, cavity or tooth which has been prepared should be clean from dirt. The researchers suggested that clean dentin surface would reach effective adhesion between resin and tooth structure, therefore dentin conditioner like citric acid was used to reach the condition. Even though citric acid is not strong acid but it can be very erosive to oral mucous. Several requirements should be fulfilled for dental product such as non toxic, non irritant, biocompatible and should not have negative effect against local, systemic or biological environment. Cytotoxicity test was apart of biomaterial evaluation and needed for standard screening. Purpose: This study was to know the cytotoxicity of 40, 50, 60% citric acid as dentin conditioner using MTT assay. Method: This study is an experimental research using the Post-Test Only Control Group Design. Six samples of each 40, 50 and 60% citric acid for citotoxicity test using MTT assay. The density of optic formazan indicated the number of living cells. All data were statistically analyzed by one way ANOVA. Result: The percentage of living cells in 40, 50 and 60% citric acid were 95.14%, 93.42% and 93.14%. Conclusion: Citric acid is non toxic and safe to be used as dentine conditioner.
Cytotoxicity difference of 316L stainless steel and titanium reconstruction plate Sumarta, Ni Putu Mira; Danudiningrat, Coen Pramono; Rachmat, Ester Arijani; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 44, No 1 (2011): (March 2011)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v44.i1.p7-11

Abstract

Background: Pure titanium is the most biocompatible material today and used as a gold standard for metallic implants. However, stainless steel is still being used as implants because of its strength, ductility, lower price, corrosion resistant and biocompatibility. Purpose: This study was done to revealed the cytotoxicity difference between reconstruction plate made of 316L stainless steel and of commercially pure (CP) titanium in baby hamster kidney-21 (BHK-21) fibroblast culture through MTT assay. Methods: Eight samples were prepared from reconstruction plates made of stainless steel type 316L grade 2 (Coen’s reconstruction plate®) that had been cut into cylindrical form of 2 mm in diameter and 3 mm long. The other one were made of CP titanium (STEMA Gmbh®)) of 2 mm in diameter and 2,2 mm long; and had been cleaned with silica paper and ultrasonic cleaner, and sterilized in autoclave at 121° C for 20 minutes.9 Both samples were bathed into microplate well containing 50 μl of fibroblast cells with 2 x 105 density in Rosewell Park Memorial Institute-1640 (RPMI-1640) media, spinned at 30 rpm for 5 minutes. Microplate well was incubated for 24 and 48 hours in 37° C. After 24 hours, each well that will be read at 24 hour were added with 50 μl solution containing 5mg/ml MTT reagent in phosphate buffer saline (PBS) solutions, then reincubated for 4 hours in CO2 10% and 37° C. Colorometric assay with MTT was used to evaluate viability of the cells population after 24 hours. Then, each well were added with 50 μl dimethyl sulfoxide (DMSO) and reincubated for 5 minutes in 37° C. the wells were read using Elisa reader in 620 nm wave length. Same steps were done for the wells that will be read in 48 hours. Each data were tabulated and analyzed using independent T-test with significance of 5%. Results: This study showed that the percentage of living fibroblast after exposure to 316L stainless steel reconstruction plate was 61.58% after 24 hours and 62.33% after 48 hours. And after exposure to titanium reconstruction plate, the percentage of living fibroblast was 98.69% after 24 hours and 82.24% after 48 hours. Based on cytotoxicity parameter (CD50%), both reconstruction plate made of 316L stainless steel or titanium showed as a non-toxic materials to fibroblast. Conclusion: Both reconstruction plate made of stainless steel and CP titanium were non-toxic to fibroblast, although the stainless steel plate showed lower cytotoxicity level compared to titanium. Therefore a reconstruction plate made from stainless steel type 316L can be used as a safe material for mandibular reconstruction. Latar belakang: Titanium murni adalah bahan yang paling biokompatibel saat ini dan digunakan sebagai standar emas implan logam. Saat ini stainless steel masih digunakan karena kekuatan, ductility, harganya yang murah, tahan terhadap korosi dan cukup biokompatibel. Tujuan: Penelitian ini dilakukan untuk mengetahui perbedaan sitotoksisitas antara plat rekonstruksi yang terbuat dari titanium murni komersial dan plat rekonstruksi yang terbuat dari stainless steel pada kultur sel fibroblas baby hamster kidney-21 (BHK-21) menggunakan MTT assay. Metode: Delapan sampel yang masing-masing tipe 316L terbuat dari stainless steel 316L grade 2 (Coen’s reconstruction plate®) yang dipotong berbentuk silinder diameter 2 mm dan panjang 3 mm, serta yang terbuat dari titanium murni komersial (STEMA Gmbh®) diameter 2 mm dan panjang 2,2 mm; dan dibersihkan dengan kertas silika dan pembersih ultrasonik serta disterilkan dengan autoclave pada suhu 121° C selama 20 menit. Kedua sampel dimasukkan ke dalam sumur mikroplat yang mengandung 50 μl sel fibroblas dengan kepadatan 2 × 105 dalam media Rosewell Park Memorial Institute-1640 (RPMI-1640), diputar dengan kecepatan 30 rpm selama 5 menit. Sumur mikroplat diinkubasi selama 24 dan 48 jam pada suhu 37° C. Setelah 24 "> jam, pada tiap sumur yang akan dibaca pada jam ke 24 ditambahkan 50 μl cairan yang mengandung 5mg/ml MTT dalam phosphat buffer saline (PBS), kemudian diinkubasi kembali selama 4 jam dalam CO2 10% pada suhu 37° C. Assay kolorimetri dengan MTT digunakan untuk mengetahui viabilitas populasi sel setelah 24 jam. Setiap sumur ditambahkan pelarut dimetil sulfoksida (DMSO) dan diinkubasi kembali selama 5 menit pada suhu 37° C. sumur-sumur tersebut kemudian dibaca dengan Elisa reader dengan panjang gelombang 620 nm. Langkah yang sama dilakukan pada sumur-sumur yang akan dibaca pada jam ke 48. Data kemudian ditabulasi dan dianalisis dengan menggunakan independent T-test dengan signifikansi 5%. Hasil: Penelitian ini menunjukkan presentase fibroblas hidup setelah terpapar plat rekonstruksi yang terbuat dari stainless steel adalah 61,58% setelah 24 jam dan 62,33% setelah 48 jam. Dan setelah paparan dengan plat rekonstruksi yang terbuat dari titanium murni adalah 98,69% setelah 24 jam dan 82,24% setelah 48 jam. Berdasarkan pada parameter sitotoksisitas (CD50%) kedua plat rekonstruksi baik yang terbuat dari titanium murni maupun yang terbuat dari stainless steel tipe 316L merupakan bahan yang tidak bersifat toksik terhadap fibroblas. Kesimpulan: Kedua plat rekonstruksi baik yang terbuat dari stainless steel maupun CP titanium tidak bersifat toksik terhadap fibroblas, walaupun plat stainless steel menunjukkan level sitotoksisitas yang lebih rendah daripada titanium murni. Dengan demikian plat rekonstruksi yang terbuat dari stainless steel 316 L aman digunakan sebagai bahan untuk rekonstruksi mandibula.
Effects of liquid ionic silver concentration on caspase-3 and p53mt expressions in the oral mucosal epithelium of Wistar rats Muharram, R. Aries; Istiati, I.; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 3 (2018): (September 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i3.p138-142

Abstract

Background: Silver, especially oxidized silver, has been used as a medicine considered to have bactericidal properties. In the present day, ionic silver (Ag+) is also used in the manufacture of cosmetics, socks, food containers, detergents, sprays and other products to prevent the spread of germs. Unfortunately, ionic silver is assumed to be toxic not only to bacteria, but also to humans and the environment. Therefore, it is essential to identify the optimum dosage of ionic silver considered safe by investigating the effects of ionic silver concentration on cell death through activation of mutant p-53 expression by caspase 3 in the oral epithelium. Purpose: This research aimed to analyze the effects of concentrated liquid ionic silver (Ag+) on caspase-3 and mutant p53 expression in the oral mucosal epithelium. Methods: This research constituted a laboratory-based experimental study with posttest-only design. The research subjects consisted of 28 Wistar rats, divided into four treatment groups, namely; KK (with Aquadest), KP 1 (with 5% liquid ionic silver), KP 2 (with 10% liquid ionic silver) and KP 3 (with 15% liquid ionic silver). Each rat was then treated orally with 0.5ml of liquid ionic silver at fixed concentrations twice a day for seven days. The Wistar rats were then terminated and their tissue samples processed by means of histopathological and immunohistochemical staining examination. The monoclonal Caspase-3 and mutant p53 expressions in each group were evaluated with the data being tabulated and analyzed statistically. Results: Mutant p53 expression was also found in the control group. Moreover, the higher the concentration of liquid ionic silver, the greater the elevated Caspase-3 and mutant p53 expressions. Conclusion:  The concentration of liquid ionic silver plays an important role in elevating Caspase-3 and mutant p53 expressions.