Vanesa Martida, Vanesa
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

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PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.) Martida, Vanesa; Pharmawati, Made
SIMBIOSIS Journal of Biological Sciences Vol 4, No 1 (2016)
Publisher : SIMBIOSIS Journal of Biological Sciences

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Abstract

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer
Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae) Martida, Vanesa; Pharmawati, Made
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2019.v03.i01.p03

Abstract

DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging.  The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali.  Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp.  This research used CTAB buffer with modification as lysis buffer.  Purification techique used NucleoSpin? Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/?l. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/?l. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/?l and need to be purified to obtain clear DNA solution.