ANAK AGUNG NGURAH GEDE SUWASTIKA, ANAK AGUNG NGURAH GEDE
Jurusan/Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana

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Analisis Kualitas Larutan Mikroorganisme Lokal Daun Gamal (Gliricidia sepium) pada Beberapa Waktu Inkubasi SUWASTIKA, ANAK AGUNG NGURAH GEDE; SUTARI, NI WAYAN SRI; MURIANI, NI WAYAN
Agrotrop: Journal on Agriculture Science Vol 5, No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Analysis of Local Microorganism Leaf Gamal (Gliricidia Sepium) Solution Quality onthe Fermentation Period. The study aims to determine the quality of local microorganismsolution under the influence of concentration leaves of Gliricidia (Gliricidia sepium) andfermentation period. This research was conducted at the Soil Laboratory, Faculty ofAgriculture, UdayanaUniversity. The design of this research used a randomized block designfactorial pattern of two factors. The first factor was the concentration of Gliricidia leavesconsisted of 0, 100, 300 g, and the second factor was the fermentation period, consists of 1,2,and 3 weeks. Results of statistical analysis showed a highly significant interaction betweenthe concentration factor and fermentation period of Gliricidia leaves the parameters observed.Concentration of 30% (300 g) Gliricidia leaves with a two-week fermentation period has thebest effect on the quality of the biological properties of microorganisms local solution with atotal population of bacteria (9.5 x 107 SPK mL-1) and total fungus (1.9 x 106 SPK mL-1),while the best quality chemical properties of the solution of local microorganisms present in aconcentration of 30% (300 g) gliricidia leaves with a three-week fermentation period with thecontent of total-N (1.59%), pH (6.03), and organic-C (3.35%). Concentration of 30% (300 g)Gliricidia leaves with fermentation period of two weeks, it can be used as a biologicalfertilizer and a concentration of 30% (300 g) Gliricidia leaves with a three-week fermentationperiod, could be used as organic manure.
ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE Suada, I Ketut; Suwastika, Anak Agung Ngurah Gede
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v04.i02.p01

Abstract

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.