Coen Pramono D, Coen Pramono
Department of Oral & Maxillofacial Surgery, Faculty of Dental Medicine, Airlangga University, Surabaya-Indonesia

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The role of ubiquinone supplementation on osteogenesis of nonvascularized autogenous bone graft Taufiqurrahman, Irham; Harijadi, Achmad; Simanjuntak, Roberto M.; D, Coen Pramono; Istiati, Istiati
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i2.p59-63

Abstract

Background: Ubiquinone is one of food supplement which is known have positive effect in wound healing. However the study to evaluate the possible role of ubiquinone in bone healing in autogenous bone grafting after mandibular resection has not been studied. An in vitro study is required to evaluate whether ubiquinone or coenzyme Q-10 (CoQ10) has a positive effect on osteogenesis. Viability test of CoQ10 and a model of osteogenic-induced and hypoxic-condition mesenchymal stem cell culture were established to support the study. Purpose: The study was made to evaluate the role of ubiquinone in osteogenesis by analyzing the toxicity effect and the optimal dose of CoQ10 that might interfere in bone marrow derived mesenchymal stem cell (BM-MSC) that was dose in cell culture medium. The BM-MSC culture under hypoxia condition were also observed. Method: The toxicity and the optimum viability concentration of ubiquinone were observed using MTT assay. The osteogenic differentiation under hypoxic condition was done on BM-MSC in osteogenic medium that composed of ascorbic acid, glycerolphosphate and dexamethasone in hypoxia chamber for 21 days. Osteogenic differentiation and cellular hypoxia features were tested with immunocytochemical staining using anti-Runx2 and anti-HIF1α monoclonal antibody, respectively. Result: The maximum density value of  1.826 was found in the group of ubiquinone concentration of 75μM/ml, increasing of in concentration of ubiquinone resulted with the decrease ofoptical density of CoQ10. Statistic analysis using Anova showed with no significant difference among groups with various concentration. Immunocytochemical staining showed that Runx2 expression in 3% hypoxia group (p<0.05). Conclusion:Ubiquinone was found non toxic in its optimum dose of 75μM/ml, showed by optimum result in the expression ofRunx2 and HIF1α further study is necessary to evaluate the angiogenic and osteogenic effect ofubiquione.
Placement of fin type dental implant in three different surgical situations of alveolar bone D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 40, No 1 (2007): (March 2007)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v40.i1.p49-53

Abstract

Three different dental implant placements according to surgical implant bed situations were observed in its bone integration 3 months after dental implant insertion. This observation was done on implant system which has plateau or fin system. Elf implants were placed in the upper jaw in two patients. In case one, two implants were inserted immediately after tooth extraction, and the other six implants were placed in the alveolar crest regions in delayed implantation or in which the teeth had been extracted over 6 months of period. In case two, three implants were inserted in the post trauma region in the anterior maxilla, which the labial plate had been lost and reconstructed with bone grafting procedure using a mixture of alloplastic and autogenous bones. The alveolar reconstruction was needed to be performed due to only thin alveolar crest width was left intact. All of those implants observed showed in good integration.
Chondrogenic Differentiation Capacity of Human Umbilical Cord Mesenchymal Stem Cells with Platelet Rich Fibrin Scaffold in Cartilage Regeneration (In Vitro Study) Sumarta, Ni Putu Mira; D, Coen Pramono; Hendrianto, Eryk; Susilowati, Helen; Karsari, Deya; Rantam, Fedik A.
BALI MEDICAL JOURNAL Vol 5, No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Background: Human umbilical cord mesenchymal stem cell is a promising source of allogenous MSC with great chondrogenic differentiation capacity. Meanwhile, platelet rich fibrin (PRF) is a natural fibrin matrix, rich in growth factors, forming a smooth and flexible fibrin network, supporting cytokines and cell migration, thus can be used as a scaffold that facilitate the differentiation of MSC. However, the differential capability of MSC cultured in PRF was still poorly understood. Method: We studied in vitro differentiation potential of MSC cultured in PRF by evaluating several markers such as FGF 18, Sox 9, type II collagen, aggrecan in 3 different culture medium. Result: The result showed that there was positive expression of FGF 18, Sox 9, type II collagen, aggrecan in all medium of in vitro culture. Conclusion: MSC cultured from human umbilical cord had the capacity of chondrogenic differentiation and able to produce cartilage extracellular matrix in vitro which means that hUCMSC is a potential allogeneic MSC for cartilage regeneration.
Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials Sularsih, Sularsih; Yuliati, Anita; D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 1 (2012): (March 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v45.i1.p17-21

Abstract

Background: Chitosan is biomaterial improved for various dentistry applications because it is biocompatible, degradable, nontoxic, and not carcinogenic. The main parameter affecting the characteristics of chitosan is deacetylation degree. Purpose: This study is aimed to determine the degree of deacetylated of chitosan derived from white shrimp shell waste used as dental biomaterial. Methods: White shrimp shells were crushed into powder. Next, deproteination process was conducted with 3.5% NaOH solution, demineralized with 1N HCl solution, and then depigmented with 90% acetone solution into chitin powder. Deacetylation process was then conducted by soaking the chitin powder in 50% NaOH solution for 6 h at 65° C to produce white powder of chitosan. Afterwards, deacetylation degree test was conducted by using Fourier Transform Infrared Spectrophotometer (FTIR) to calculate the ratio of the absorption bands between the absorbance peak of amide group about 1655 cm–1 and the absorbance peak of hydroxyl group about 3450 cm–1. Results: The result of the deacetylation degree test on the chitosan powder derived from white shrimp shell waste was high, about 85.165%, and had the eligible form, solubility, and pH. Conclusion: It can be concluded that the deacetylation degree of chitosan from white shrimp shells could reach 85.165%.Latar belakang: Kitosan merupakan biomaterial yang dikembangkan untuk berbagai aplikasi kedokteran gigi karena biokompatibel, dapat didegradasi, tidak toksik dan tidak karsinogenik. Parameter utama yang mempengaruhi karakteristik kitosan adalah derajat deasetilasi. Tujuan: Tujuan dari penelitian ini adalah mengetahui derajat deasetilasi kitosan dari limbah kulit udang putih sebagai biomaterial kedokteran gigi. Metode: Kulit udang putih dihaluskan menjadi serbuk. Setelah itu dilakukan proses deproteinasi dengan larutan NaOH 3,5%, demineralisasi dengan larutan HCl 1N, depigmentasi dengan larutan aseton 90% sehingga menjadi serbuk kitin. Proses deasetilasi dilakukan dengan merendam serbuk kitin dalam larutan NaOH 50% selama 6 jam pada suhu 65° C sehingga dihasilkan serbuk putih kitosan. Uji derajat deasetilisasi menggunakan metode spektrofotometer Fourier Transform Inframerah (FTIR) dengan menghitung nilai perbandingan pita serapan antara puncak absorbansi gugus amida sekitar 1655 cm–1, dan puncak absorbansi gugus hidroksil sekitar 3450 cm–1. Hasil: Hasil uji derajat deasetilasi serbuk kitosan dari limbah kulit udang putih adalah tinggi yaitu sebesar 85.165% dan memiliki bentuk, kelarutan dan pH yang memenuhi syarat. Kesimpulan: Dapat disimpulkan derajat deasitilasi kitosan dari kulit udang putih adalah 85,165%.
Problem in the surgical correction of long-face with vertical open bite D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 4 (2005): (December 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v38.i4.p159-168

Abstract

Long-face cases usually need both treatment of orthodontic and surgery. The problem appearing in the correction of long-face might be able to be related with some difficult factors such as the crowded teeth and excessive vertical height. A class III malocclusion and excessive open bite can be also followed in long face. This situation might worsen the facial aesthetic condition and increase the difficulty in orthodontic treatment. The orthodontic approach is oriented toward positioning the teeth pre-surgically to facilitate the surgical plan. The form of mandible which has grown in the downward direction in the area of mandible angle makes an extreme vertical open bite. The maxilla is usually presented with a maxillary hypolasia. Double-jaw surgery was done as the correction of the lower jaw alone would produce a flattened face appearance and difficulty in repositioning the mandible to achieve a good facial performance. Several cephalometric points were measured to observe the facial situation progress after surgery. Two cases of longface are reported, and the same surgical treatments were performed and showed different results.
Lip and allar base dimensions among adolescence in healthy and post unilateral cleft lips correction in deutro-malaid population D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 39, No 1 (2006): (March 2006)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v39.i1.p1-7

Abstract

The purpose of this study is to observe the differences between some anthropometric values of the lip and allar base area in normal and those who had undergone incomplete unilateral cleft lip surgical correction using Millard’s I technique. The investigation was done on male und female adolescence of 12–15 years of age. Thirteen anthropometric dimension variables were observed, as X1 = width of the allar base, X2 = the distance between two corners of the mouth, X3 = the distance between the center of the lip and the right mouth corner, X4 = the distance between the center of the lip and the left mouth corner, X5 = the distance between the center of the allar base and the center of the of upper lip, X6 = the distance between upper part of two philtrum pillars, X7 = the distance between two philtrum pillars base, X8 = the height of right philtrum pillar, X9 = the height of left philtrum pillar, X10 = the distance between the right allar base to the right mouth corner, X11 = the distance between the left allar base to the left mouth corner, X12 = the width of the right nostril, and X13 = the width of the left nostril. The t-test analysis showed the average of the anthropometric lip and allar base dimensions among male adolescence groups in variables of X1, X2, X4, X5, X6, X7, X10, X11, X12, and X13 shown with significant differences from those of some variables which had undergone incomplete unilateral cleft lip surgical correction (p < 0.05), and no significant differences shown in the variables of X3, X6, X8, and X9 (p > 0.05). The result of the test among the female group presented a significant difference in variables of X4, X5, X6, X9, X11, X12 and X13 (p < 0.05), differed what given in variables of X1, X2, X3, X7 and X8 showed with no significant difference (p > 0.05). It concluded that there are differences in dimensions of the lips and allar bases among normal and post-surgical groups both in adolescence males and females.
Management of zygomatic-maxillary fracture (The principles of diagnosis and surgical treatment with a case illustration) Kamadjaja, David B.; D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 2 (2008): (June 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v41.i2.p77-83

Abstract

Mechanical trauma to the face may cause complex fracture of the zygoma and the maxilla. The characteristic clinical signs of zygomatic bone fracture include flattening of the cheek, infraorbital nerve paraesthesia, diplopia, and trismus, whereas maxillary fracture may typically cause flattening of the midface and malocclusion. The diagnosis of zygomatic and maxillary fracture should be established with thorough clinical examination and careful radiologic evaluation so that a three-dimensional view of the fractured bones can be obtained. This is essential in order to plan a proper surgical treatment to reconstruct the face in terms of functions and aesthetic. A standard surgical protocol should also be followed in performing the surgical reconstruction of the zygoma and the maxilla. A case of delayed bilateral fracture of zygoma and maxilla is presented here to give illustration on how the principle of diagnosis and surgical treatment of complex zygomatico-maxillary fracture are applied.
Minor modification of Millards surgical technique for correction of complete unilateral cleft lip D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 43, No 4 (2010): (December 2010)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v43.i4.p172-175

Abstract

Background: A surgical technique for correction of complete unilateral cleft lip was done using a minor modification of Millard’s surgical technique. The purpose of this modification is to achieve a good anatomical form of columelia, nostril cill and the position of nasal tip. Purpose: This article presents the correction of the complete cleft lip which was done initially by correction of the slanted columella followed by correction of the nostril sill which was done before the sequence of closing the lip crevice. Case: Correction of a case with complete unilateral cleft lip on a fifteen year old girl using modification of Millard’s surgical technique is presented. Case Management: Rotation incision in the philtrum region was made as introduced by Millard to make a triangular flap. The triangular flap was contralaterally rotated and pulled into the direction of cleft to achieve a normal position of the columella and nasal tip. The lateral side of the ala was incised in circular form down to the alar base and straight through to the direction of cleft formed an alar flap which consisted of ala, clefted and slanted nasal base tissue. The tip of the triangular flap was trimmed and approximated to the alar flap to form a new the nostril sill. Adjustment of the size of the nostrill sill size was achieved during the approximation of those two flaps. The nasal base was built during approximation of the lateral and the medial segment flap or philtrum region and the base of new nostril sill. Surgical correction of the complete unilateral cleft lip including correction of the nostrill sill using approximation of triangular flap and the alar flap was achieved. Conclusion: This surgical technique with minor modification of Millard’s surgical technique can be used for correction of the complete unilateral cleft lip with extremely slanted columella and nasal tip to form thenostril sill.Latar belakang: Koreksi celah bibir komplit satu sisi telah dilakukan menggunakan metode operasi Millard yang dimodifikasi. Modifikasi dimaksudkan untuk memperoleh bentuk anatomis yang baik dari columella, nostril dan letak ujung hidung yang baik. Tujuan: Melaporkan koreksi suatu celah bibir komplit satu sisi menggunakan metode operasi Millard yang dimodifikasi yang dimulai dengan melakukan perbaikan pada columella yang miring pada bentuk nostril sebelum tahapan penutupan celah bibir dilakukan. Kasus: Dilaporkan satu kasus koreksi celah bibir komplit satu sisi pada pasien wanita usia 15 tahun menggunakan metode operasi Millard yang dimodifikasi. Tatalaksana kasus: Dilakukan insisi berbentuk rotasi di regio seperti pada metode Millard untuk membuat flap berbentuk trianguler. Flap trianguler dirotasikan ke arah kontra lateral dan ditarik menuju arah celah bibir untuk memperoleh posisi columella yang normal, bentuk nostril yang simetris dan letak ujung hidung yang baik. Dilakukan insisi sirkuler ke bawah ke arah dasar ala pada regio lateral ala menuju ke bagian celah bibir, selanjutnya akan diperoleh bentuk flap yang disebut alar flap yang terdiri atas bagian ala nasi, bagian dari jaringan yang berada pada sisi celah dan bagian dari dasar hidung di sisi celah bibir. Bagian ujung dari trianguler flap dipotong dan selanjutnya ditautkan pada flap alar untuk membentuk bentukan nostril baru. Penyesuaian ukuran nostril dilakukan pada tahap penautan kedua flap tersebut. Bagian dari dasar hidung dibentuk pada saat tahapan penautan antara flap segmen lateral dan medial atau bagian dari philtrum dan bagian dasar dari nostril atau cuping hidung. Koreksi bedah suatu celah bibir komplit satu sisi termasuk koreksi pada bentuk nostril diperoleh dengan menautkan antara flap trianguler dan flap alar. Kesimpulan: Teknik bedah Millard dengan modifikasi kecil dapat dipakai untuk melakukan koreksi pada kasus celah bibir komplit satu sisi yang disertai kemiringan columella dan ujung hidung yang ekstrim.
Placement of replace select Ti-Unite-coated type implants using a combination of immediate and submerge techniques after tooth extraction D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 39, No 2 (2006): (June 2006)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v39.i2.p48-53

Abstract

The high success rate of immediate implant placement both in the anterior and posterior regions were reported by many authors, therefore applying this techniques can be considered as a safe surgical procedure and minimizing the dental office visit for patient satisfaction. This paper reports the outcome of immediate placement of implants following extraction of anterior maxillary teeth. Combination technique of immediate and submerge implant placements including bone grafting procedure were used. Four implants with TiUnite surface type were placed immediately in two patients with the short-term result indicated that this technique may serve as a simple and safe procedure for immediate implant placement. It was concluded that immediate implant placement technique combined with TiUnite implant surface was successful in treating region directly after tooth extraction therefore this technique can be use as an alternative surgical method for dental implant rehabilitation.
The advantage of the three dimensional computed tomographic (3 D-CT) for ensuring accurate bone incision in sagittal split ramus osteotomy D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 1 (2005): (March 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v38.i1.p1-3

Abstract

Functional and aesthetic dysgnathia surgery requires accurate pre-surgical planning, including the surgical technique to be used related with the difference of anatomical structures amongst individuals. Programs that simulate the surgery become increasingly important. This can be mediated by using a surgical model, conventional x-rays as panoramic, cephalometric projections and another sophisticated method such as a three dimensional computed tomography (3 D-CT). A patient who had undergone double jaw surgeries with difficult anatomical landmarks was presented. In this case the mandible foramens were seen highly relatively related to the sigmoid notches. Therefore, ensuring the bone incisions in sagittal split was presumed to be difficult. A 3D-CT was made and considered to be very helpful in supporting the pre-operative diagnostic.