Saptowo Jumali Pardal, Saptowo Jumali
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar 3A, Bogor 16111

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Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293 Tasma, I Made; Warsun, Ahmad; Satyawan, Dani; Pardal, Saptowo Jumali; Slamet, Slamet
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.856 KB)

Abstract

Genetic Mapping of SSR Markers in Eight SoybeanChromosomes Based on F2 Population B3462 x B3293. IMade Tasma, Ahmad Warsun, Dani Satyawan, SaptowoJ. Pardal, and Slamet. Aluminum toxicity is one of the maincontrains for cultivating soybean in acid soils. GeneticHak Cipta © 2011, BB-Biogenmapping of SSR markers is one step for detecting aluminumtoxicitytolerant QTLs in soybean. Another step is tophenotype the same population at various aluminum-toxicityenvironments. The objectives of this study were to analyzethe segregation of SSR markers in progenies of an F2population and map the markers in 8 soybean chromosomes.The F2 population was previously developed bycrossing the Al-tolerant parent B3462 and the Al-sensitiveparent B3293. Polymorphic SSR markers in the parents wereused to PCR amplify DNA of the 100 F2 progenies. PCRproducts were separated using agarose or polyacrylamidegels. A Chi-Square test was done with a null hypothesis thatprogenies segregated in a 1 : 2 : 1 ratio. Results showed that125 SSR markers were polymorphics in the parents. Out of125 polymorphic markers, 122 were segregated in theprogenies of the F2 population. Among the segregatingmarkers, 114 were segregated in a 1 : 2 : 1 ratio. Only 8markers (5.6%) did not follow the 1 : 2 : 1 ratio. One hundredand nineteen SSR markers were mapped in 8 soybeanchromosomes. These include 18 markers in chromosomeA2, 10 in B1, 16 (C1), 16 (F), 10 (G), 23 (J), 16 (L), and 10 (N).Total genetic maps covered was 1,194.8 cM with averagemap distances between two adjacent markers of 10.7 cM.Further SSR marker enrichment is required to fill in the gapsof several chromosomal regions. Genetic maps presented inthis study should be useful for detection of Al-toxicitytolerant QTLs in soybean.
Biostimulasi pertumbuhan vegetatif tanaman tebu (Saccharum officinarum L.) pada fase awal di lahan kering (Biostimulation of vegetative growth of sugarcane (Saccharum officinarum L.) in the initial phase on dry land) WAHYUNI, Sri; HABIBULLAH, Hanning Susilo; PUTRA, Soekarno Mismana; AMANAH, Dian Mutiara; SISWANTO, .; PRIYONO, .; SANTOSO, Djoko; PARDAL, Saptowo Jumali
E-Journal Menara Perkebunan Vol 86, No 2 (2018): Oktober 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v86i2.284

Abstract

AbstractThe expansion of sugarcane areas as a support to national sugar production has shifted to sub-optimal dry land. In drought stress conditions, early growth of sugarcane usually can inhibite and decrease its productivity. This study aimed to test the efficacy of organic biostimulant in increasing vegetative growth of sugarcane in the dry land. Firstly, seedlings were submerged with biostimulant of Citorin-Rfor overnight. Secondly, the biostimulant application of Citorin-S was carried out by foliar sprayat age1 and4 months old trees. Humicacid 0.5% (v/v) was applied in soil before planting while the application of mycorrhiza was carried out by direct pouring on soil during planting. The results showed that the initial vegetative growth of biostimulant-treated sugarcane stem diameter and length were 23% wider and 27% higher compared to that of control, respectively. In subsequent growth cycle, all observed vegetative parameters showed higher growth value in the biostimulant-treated sugarcanes than that of the control. Plant height, stem diameter and number of tillers of biostimulant-treated sugarcanes had significantly higher values than that of the control. P3 treatment (organic biostimulant plus humic acid and mycorrhiza) was the best treatment. The height and diameter of P3 sugarcane stems were 47% wider and 59% higher, respectively, compared to that of control at 107 DAP.[Keywords: biostimulant, plant height, stem diameter, number of tillers, number of leaves] Abstrak Penambahan areal tanaman tebu untuk mendukung peningkatan produksi gula nasional telah bergeser ke areal sub-optimal lahan kering. Pada kondisi cekaman kekeringan, pertumbuhan awal tebu biasanya terhambat dan dapat menurunkan produktivitas saat panen. Penelitian ini bertujuan menguji efikasi biostimulan organik untukmeningkatkan pertumbuhan vegetatif tanaman tebu pada fase awal di lahan kering. Perlakuan biostimulan Citorin-R diaplikasikan pada benih dengan cara perendaman semalam. Perlakuan kedua, biostimulan Citorin-S disemprotkanpada saat tanaman tebu berumur 1 dan 4 bulan secara foliar spray. Aplikasi asam humat 0,5 % (v/v) di tanah dilakukan sebelum tanam, sedangkan aplikasi mikoriza dilakukan dengan pemberian langsung pada tanah saat penanaman bagal tebu. Hasil penelitian menunjukkan bahwa nilai pertumbuhan vegetatif awal tanaman tebu perlakuan memiliki diameter batang sekitar 23% dan tinggi tanaman 27% lebih tinggi daripada tebu kontrol. Pada pertumbuhan selanjutnya, semua parameter vegetatif yang diamati menunjukkan nilai pertumbuhan yang lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Tinggi tanaman, diameter batang dan jumlah anakan secara statistik berbeda nyata lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Perlakuan P3 (biostimulan organik plus asam humat dan mikoriza) adalah perlakuan terbaik. Tinggi dan diameter batang tanaman tebu P3 masing-masing 47% dan 59% lebih besar daripada batang tanaman kontrol pada 107 hari setelah tanam (HST).  [Kata kunci :biostimulan, tinggi tanaman, diameter batang, jumlah anakan, jumlah daun]
Pengaruh biostimulan terhadap pertumbuhan vegetatif tanaman tebu varietas PSJT-941 [Effects of biostimulants on vegetative growth of sugarcane variety PSJT-941] PUTRA, Soekarno Mismana; SUSANTI, Paramitha; AMANAH, Dian Mutiara; UMAHATI, Binti Khurotul; PARDAL, Saptowo Jumali; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 85, No 1 (2017): April, 2017
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.745 KB) | DOI: 10.22302/iribb.jur.mp.v85i1.241

Abstract

 A plant biostimulan made of local seaweed, Citorin, has been developed and tested increase productivity some seasonal crops such as rice, maize, soybean, chilli and onion. The research aimed to evaluate the effects of the biostimulant application on vegetative growth and productivity of sugarcane varieties PSJT-941 in polybag. The biostimulant was applied at three different stages of development of the sugarcane. With or without the addition of humic acid based biostimulan or mycorrhiza. Biostimulant-R was applied at the time the cane seedlings just before planted by by soaking in 100 ppm biostimulan for overnight, plant sugarcane leaves sprayed aged 1 month to 10 ppm Biostimulant-S much 25 ml per plant, 4 months old foliar sprayed with Biostimulant-S 10 ppm as 120 ml per plant. Of the six treatments (P2 - P7) used all showed better vegetative growths that than the control plants without biostimulan (P1). The best treatment was P3, the rise in the average height of the plants reached 13%, which is a combination of soaking and spraying Biostimulant-S 10 ppm. The following best were the treatment of P6 and P5 those were using a combination of humic acid based biostimulan plus mycorrhiza, and humic acid without mycorrhizae. Likewise, its influence on the number of tillers, P3 is the best treatment by enhancing the number of tillers on average 26% higher than the control. Next was P5 and P6. Meanwhile the influence on the average weight of harvested sugarcane, the best treatment is P7 reached 1.25 kg / per sugarcane or increased 47.1%. Next is the treatment of P4 and P6. As for the effect on the sugar content, the best treatment is P4 reached 11.2 % Bix per sugarcane or increased 13.1 %. Next is the treatment of P5 and P7. Based on the results of the assessment scoring system of three parameters the rooting, weight and sugar yield, the best treatment is the treatment of P5 and P4 P7 later, each with a total score of 13, 12, and 10. [Keywords: Plant biostimulants, productivity, sugar yield, Saccharum officinarum]AbstrakBiostimulan tanaman berbasis rumput laut lokal, Citorin, telah dikembangkan dan diuji meningkatkan produktivitas beberapa tanaman pangan semusim antara padi, jagung, kedelai, cabe, dan bawang merah. Tujuan penelitian ini adalah meneliti pengaruh aplikasi biostimulan tersebut pada pertumbuhan vegetatif dan produktivitas tanaman tebu varietas PSJT-941 di polibeg. Biostimulan diaplikasi pada tiga tingkat perkembangan yang berbeda dari tanaman tebu. Dengan atau tanpa penambahan biostimulan berbasis asam humat atau mikoriza, Biostimulan-R, diaplikasikan pada saat bibit tebu yang akan ditanam, direndam terlebih dahulu dalam dosis 100 ppm selama semalam, tanaman tebu umur 1 bulan disemprot daun dengan Biostimulant-S 10 ppm sebanyak 25 ml per tanaman, umur 4 bulan disemprot daun dengan Biostimulan-S 10 ppm sebanyak 120 ml per tanaman. Dari enam perlakuan (P2 – P7) yang digunakan semuanya menunjukkan pertumbuhan vegetatif yang lebih baik daripada tanaman kontrol tanpa biostimulan (P1).  Terhadap tinggi tanaman tebu umur perlakuan terbaik adalah P3, kenaikan tinggi rata-rata tanaman mencapai 13%, yaitu kombinasi perendaman dalam Biostimulan-R 100 ppm dan penyemprotan Biostimulan-S 10 ppm. Yang berikutnya adalah perlakuan P6 dan P5 yaitu menggunakan kombinasi biostimulan berbasis asam humat plus mikoriza, dan asam humat tanpa mikoriza. Demikian juga pengaruhnya terhadap jumlah anakan, perlakuan P3 adalah yang terbaik dengan peningkat jumlah anakan rata-rata mencapai 26% lebih tinggi daripada kontrol. Berikutnya adalah P5 dan P6. Sementara itu pengaruhnya terhadap rerata bobot batang tebu dipanen, perlakuan terbaik adalah P7 mencapai 1,25 kg/per batang atau naik 47,1%. Berikutnya adalah perlakuan P4 dan P6. Adapun pengaruhnya terhadap kandar gulanya, perlakuan terbaik adalah P4 mencapai 11,2 % Brix atau naik 13,1%. Berikutnya adalah perlakuan P5 dan P7.  Berdasarkan hasil penilaian dengan sistem skoring dari 3 parameter perakaran, bobot panen dan rendemen gula, maka perlakuan terbaik adalah perlakuan P7 kemudian P5 dan P4, masing-masing dengan skor total 13, 12, dan 10.                                  [Kata kunci:  Biostimulan tanaman, produktivitas, rendemen gula, Saccharum officinarum]
Biostimulasi pertumbuhan vegetatif tanaman tebu (Saccharum officinarum L.) pada fase awal di lahan kering (Biostimulation of vegetative growth of sugarcane (Saccharum officinarum L.) in the initial phase on dry land) WAHYUNI, Sri; HABIBULLAH, Hanning Susilo; PUTRA, Soekarno Mismana; AMANAH, Dian Mutiara; SISWANTO, .; PRIYONO, .; SANTOSO, Djoko; PARDAL, Saptowo Jumali
E-Journal Menara Perkebunan Vol 86, No 2 (2018): Oktober 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v86i2.284

Abstract

AbstractThe expansion of sugarcane areas as a support to national sugar production has shifted to sub-optimal dry land. In drought stress conditions, early growth of sugarcane usually can inhibite and decrease its productivity. This study aimed to test the efficacy of organic biostimulant in increasing vegetative growth of sugarcane in the dry land. Firstly, seedlings were submerged with biostimulant of Citorin-Rfor overnight. Secondly, the biostimulant application of Citorin-S was carried out by foliar sprayat age1 and4 months old trees. Humicacid 0.5% (v/v) was applied in soil before planting while the application of mycorrhiza was carried out by direct pouring on soil during planting. The results showed that the initial vegetative growth of biostimulant-treated sugarcane stem diameter and length were 23% wider and 27% higher compared to that of control, respectively. In subsequent growth cycle, all observed vegetative parameters showed higher growth value in the biostimulant-treated sugarcanes than that of the control. Plant height, stem diameter and number of tillers of biostimulant-treated sugarcanes had significantly higher values than that of the control. P3 treatment (organic biostimulant plus humic acid and mycorrhiza) was the best treatment. The height and diameter of P3 sugarcane stems were 47% wider and 59% higher, respectively, compared to that of control at 107 DAP.[Keywords: biostimulant, plant height, stem diameter, number of tillers, number of leaves] Abstrak Penambahan areal tanaman tebu untuk mendukung peningkatan produksi gula nasional telah bergeser ke areal sub-optimal lahan kering. Pada kondisi cekaman kekeringan, pertumbuhan awal tebu biasanya terhambat dan dapat menurunkan produktivitas saat panen. Penelitian ini bertujuan menguji efikasi biostimulan organik untukmeningkatkan pertumbuhan vegetatif tanaman tebu pada fase awal di lahan kering. Perlakuan biostimulan Citorin-R diaplikasikan pada benih dengan cara perendaman semalam. Perlakuan kedua, biostimulan Citorin-S disemprotkanpada saat tanaman tebu berumur 1 dan 4 bulan secara foliar spray. Aplikasi asam humat 0,5 % (v/v) di tanah dilakukan sebelum tanam, sedangkan aplikasi mikoriza dilakukan dengan pemberian langsung pada tanah saat penanaman bagal tebu. Hasil penelitian menunjukkan bahwa nilai pertumbuhan vegetatif awal tanaman tebu perlakuan memiliki diameter batang sekitar 23% dan tinggi tanaman 27% lebih tinggi daripada tebu kontrol. Pada pertumbuhan selanjutnya, semua parameter vegetatif yang diamati menunjukkan nilai pertumbuhan yang lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Tinggi tanaman, diameter batang dan jumlah anakan secara statistik berbeda nyata lebih tinggi pada tanaman tebu perlakuan daripada kontrol. Perlakuan P3 (biostimulan organik plus asam humat dan mikoriza) adalah perlakuan terbaik. Tinggi dan diameter batang tanaman tebu P3 masing-masing 47% dan 59% lebih besar daripada batang tanaman kontrol pada 107 hari setelah tanam (HST).  [Kata kunci :biostimulan, tinggi tanaman, diameter batang, jumlah anakan, jumlah daun]