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Improvement on Diesel Conversion of HydroDeOxygenation Nyamplung Oil by Multi Stage H2

Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Prosiding Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

Nyamplung (Calophyllum inophyllum L.) has a high survival potency in wide variety environment, fruitingall year round and easy regeneration. Nyamplung oil is the most suitable raw material as feedstock of biofuelbecause of its high yield of the seeds and in utilization not compete with food interests. Utilization nyamplungoil for Green Diesel through two basic stages, processing into Pure Plant Oil (PPO) and PPO upgradinginto Green Diesel. PPO upgrading with target Green Diesel further divided into two stages:Hydrodeoxygenation (HDO) and isomerization. In this preliminary study, PPO upgrading is only carried outby HDO only using NiMo catalyst. HDO is done in batch processes to identify the characteristics of theprocess at a temperature of 300oC, bar and the 5% catalyst. Meanwhile, to increase the nyamplung oilconversion into diesel, then the multi-stage Hydrogen (H2) was done in the process. Stage number of H2inserting is the major parameter in this HDO process. In the multi-stage H2: 1 times, 2 times and 4 times intothe reactor. Multi-stage H2 is done by replacing the H2 gas into the reactor, after a process gas frompreviously discarded. Carboxilation and carbonilation occurs in HDO process. By multi-stage H2, it showedan increasing yield of diesel conversion. From 1x stage H2 inserting to 2x and 4x stages of H2, it improve theyield from 0.32% to 0.76% and to 1.3%. This shows that the yield HDO is determined largely by theeffectiveness of H2 and PPO contact with the catalyst therein. Unfortunately, this condition is very difficult tobe achieved at a high pressure batch reactor. Further testing is doing HDO in a continuous reactor andobtained the conversion reached a yield of 50.48%.

DESIGN OF POTENTIAL CELLULASE PRIMER USING MULTIPLE SEQUENCE ALIGNMENT METHOD

Kursor Vol 7, No 1 (2013)
Publisher : University of Trunojoyo Madura

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Abstract

DESIGN OF POTENTIAL CELLULASE PRIMER USING MULTIPLE SEQUENCE ALIGNMENT METHOD aBahrul Ulum, bWisnu Ananta Kusuma, c Joni Prasetyo a,bDepartment of Computer Science, Bogor Agricultural University, Bogor, Indonesia aDepartment of Informatics Engineering, Al-Kamal Institute of Science and Technology, Jakarta cRenewable Energy Division, BPPT, Serpong, Indonesia E-mail: aabahrul@gmail.com Abstrak Selulase mempunyai peranan utama dalam pemanfaatan limbah biomassa yang mengandung lignin, hemicellulose, dan cellulose (lignocellulose). Limbah biomassa ini sangat banyak terdapat di lingkungan dan sampai saat ini masih belum dimanfaatkan secara maksimal, dikarenakan banyak mikroorganisme dari alam yang memproduksi enzim selulase dengan jumlah terbatas (aktifitasnya rendah). Dalam rangka meningkatkan produktivitas mikroorganisme untuk menghasilkan selulase, salah satu cara yang dapat diterapkan adalah merancang primer sekuens gen penyandi selulase yang dirangkum dari beberapa mikroorganisme penghasil selulase. Dalam penelitian ini, kami melakukan penyejajaran sekuen DNA penyandi selulase untuk mencari potensial primer untuk meningkatkan produktivitas enzim selulase dengan teknik Multiple Sequence Alignment (MSA). Metode yang digunakan adalah metode progresif (Progressive Alignment Algorithms). Hasil penelitian menunjukan bahwa pada tahap penyejajaran, didapatkan tiga daerah konservatif (conserved regions). Sedangkan pada tahap perancangan dengan beberapa parameter yang telah ditentukan didapatkan 46 pasang primer dari lima sekuen gen penyandi selulase yang didapat dari National Center for Biotechnology Information (NCBI). Kata kunci: Selulase, Multiple Sequence Alignment, Perancangan Primer. Abstract The role of cellulase is very important in degrading cellulose which is abundant in the environment, such as in biomass waste that is containing lignin, hemicellulose, and cellulose. Biomass waste is abundant in the environment and is still not fully utilized, because many of the natural microorganisms that produce cellulase enzymesproduce the enzyme in a limited amount (have low activity). In order to improve the productivity of microorganisms in producing cellulase, one of the ways that can be applied is to design primer sequences of genes encoding cellulase summarized from several cellulase-producing microorganisms. In this research,we perform alignment of DNA sequences coding of cellulase to look for potential primer in order to increase the productivity of cellulase enzymes by Multiple Sequence Alignment (MSA) method. The method used is progressive (Progressive Alignment Algorithms). The results showed that in the alignment phase, three conserved regions were obtained. However, in the planning phase by using some predetermined parameters 46 pairs of primer sequences were obtained from five genes encoding cellulase taken from NCBI. Keywords: Cellulase, Multiple Sequence Alignment, Primer design.

DESIGN OF POTENTIAL CELLULASE PRIMER USING MULTIPLE SEQUENCE ALIGNMENT METHOD

Kursor Vol 7, No 1 (2013)
Publisher : University of Trunojoyo Madura

Show Abstract | Original Source | Check in Google Scholar

Abstract

DESIGN OF POTENTIAL CELLULASE PRIMER USING MULTIPLE SEQUENCE ALIGNMENT METHOD aBahrul Ulum, bWisnu Ananta Kusuma, c Joni Prasetyo a,bDepartment of Computer Science, Bogor Agricultural University, Bogor, Indonesia aDepartment of Informatics Engineering, Al-Kamal Institute of Science and Technology, Jakarta cRenewable Energy Division, BPPT, Serpong, Indonesia E-mail: aabahrul@gmail.com Abstrak Selulase mempunyai peranan utama dalam pemanfaatan limbah biomassa yang mengandung lignin, hemicellulose, dan cellulose (lignocellulose). Limbah biomassa ini sangat banyak terdapat di lingkungan dan sampai saat ini masih belum dimanfaatkan secara maksimal, dikarenakan banyak mikroorganisme dari alam yang memproduksi enzim selulase dengan jumlah terbatas (aktifitasnya rendah). Dalam rangka meningkatkan produktivitas mikroorganisme untuk menghasilkan selulase, salah satu cara yang dapat diterapkan adalah merancang primer sekuens gen penyandi selulase yang dirangkum dari beberapa mikroorganisme penghasil selulase. Dalam penelitian ini, kami melakukan penyejajaran sekuen DNA penyandi selulase untuk mencari potensial primer untuk meningkatkan produktivitas enzim selulase dengan teknik Multiple Sequence Alignment (MSA). Metode yang digunakan adalah metode progresif (Progressive Alignment Algorithms). Hasil penelitian menunjukan bahwa pada tahap penyejajaran, didapatkan tiga daerah konservatif (conserved regions). Sedangkan pada tahap perancangan dengan beberapa parameter yang telah ditentukan didapatkan 46 pasang primer dari lima sekuen gen penyandi selulase yang didapat dari National Center for Biotechnology Information (NCBI). Kata kunci: Selulase, Multiple Sequence Alignment, Perancangan Primer. Abstract The role of cellulase is very important in degrading cellulose which is abundant in the environment, such as in biomass waste that is containing lignin, hemicellulose, and cellulose. Biomass waste is abundant in the environment and is still not fully utilized, because many of the natural microorganisms that produce cellulase enzymesproduce the enzyme in a limited amount (have low activity). In order to improve the productivity of microorganisms in producing cellulase, one of the ways that can be applied is to design primer sequences of genes encoding cellulase summarized from several cellulase-producing microorganisms. In this research,we perform alignment of DNA sequences coding of cellulase to look for potential primer in order to increase the productivity of cellulase enzymes by Multiple Sequence Alignment (MSA) method. The method used is progressive (Progressive Alignment Algorithms). The results showed that in the alignment phase, three conserved regions were obtained. However, in the planning phase by using some predetermined parameters 46 pairs of primer sequences were obtained from five genes encoding cellulase taken from NCBI. Keywords: Cellulase, Multiple Sequence Alignment, Primer design

Pengaruh Kondisi Operasi pada Pembuatan Green Diesel dari MinyakNyamplung dengan Katalis NiMo/Al2O3 dan NiW/Al2O3

Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Prosiding Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

The attractiveness of biodiesel as an alternative fuel compared to fossil fuels because it has many advantages such as the availability of abundant raw materials, more environmentally friendly, high combustion efficiency, low sulfur content, high cetane number and biodegradability. Making biodiesel from callophylum inophyllum oil has been done through the hydrogenation process. Callophylum inophylum oil was treated via degumming and neutralisation to remove all impurities before hydroprocesing. Hydroprocessing was carried out in a 500ml autoclve at 30 – 50 MPa of initial hydrogen pressure, 300 – 400 oC of reaction temperature and equiped with stirrer and cooling sistem. NiMo/Al2O3 or NiW/Al2O3 was activated with CS2 mixture at 370 oC prior to the reaction. Some physical and chemical properties of the catalytic hydroprocessing product have been investigated in accordance to ASTM standard. The measurement result of product varies according to the operation condition. The result showed that calophyllum inophullum oil can be used as raw material for green diesel over NiMo/Al2O3 and NiW/Al2O3. Sulfided NiMo/Al2O3 catalysts are preferred due to high diesel yield.

Optimasi Produksi Biohidrogen dari Palm Oil Mill Effluent dengan Metode Suppressing Mikroba Metanogenik pada Inokulum Kotoran Sapi

Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2018: PROSIDING SNTKK 2018
Publisher : Prosiding Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

Research in the field of bioenergy technology is currently being developed. One of bioenergy types that became the focus of development in Indonesia is biohydrogen. In producing biohydrogen from palm oil mill effluent (POME) using inoculum from cow dung, the production of biomethane gas causes biohydrogen productivity being decreased. The method used to increase the productivity of biohydrogen in this study is by suppressing methanogenic microbial treatment method which is conducted by heating cow dung inoculum at 95 °C for 120 min. Producing biohydrogen was done by fermented both POME and cow dung inoculum in vial bottle 120 mL at room temperature. Fermentation condition was adjusted at pH 5.5 by phosphoric buffer 1 M. The results obtained in this study consist with the increasing value of biohydrogen productivity, maximum of cummulative biohydrogen production rate and yield were 75.61 % and 0.43 mL H2/hr and 104.82 mL H2/g COD, respectively. This method was also perfectly suppressed biomethane gas production with percentage decreasing value is 100 %. The aim of this study is to investigate the effectiveness of suppressing method in optimizing biohydrogen production.

STUDI PEMANFAATAN MINYAK JELANTAH SEBAGAI BAHAN BAKU PEMBUATAN BIODIESEL

Jurnal Ilmiah Teknik Kimia Vol 2, No 2 (2018): Jurnal Ilmiah Teknik Kimia
Publisher : Program Studi Teknik Kimia, Universitas Pamulang

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Abstract

ABSTRAK Pemanfaatan minyak jelantah sebagai bahan baku pembuatan biodiesel memberikan beberapa manfaat seperti mengurangi pencemaran lingkungan di began air. Karena masyarakat sekarang ini cenderung membuang minyak jelantah dengan kualitas yang sangat rendah. Selain itu, bahan baku minyak jelantah ini sudah bukan lagi dikategorikan sebagai bahan pangan mengingat bentuknya yang hitam dan encer. Pembuatan biodiesel dengan transesterifikasi suasana basa ini bisa dilakukan dengan biaya yang murah dengan mengggunakan NaOH teknis yang banyak dijual dipasar dan ethanol teknis. Optimasi dilakukan dengan mempertimbangkan parameter jumlah NaOH teknis 2N mulai dari 10, 25, 40, 55 dan 70 ml dan exces ethanol 0, 25, 50, 75 dan 100%. Proses transesterifikasi dilakukan pada suhu 80°C selama tiga jam dalam pengadukan yang homogen. Kondisi optimum didapatkan dengan menggunakan 10 ml NaOH 2M dan 0% exces ethanol sebesar 196.64 gr/200 gr minyak jelantah. Kondisi ini juga mampu meminimalkan volume glycerol yang hanya berjumlah 79.79 ml. Secara keseluruhan FFA biodiesel ini sudah dibawah 0.5% sesuai dengan yang diharapkan. Adapun kualitas biodiesel melalui pengamatan masa jenis yang menunjukan 0.937 gr/ml masih harus dilakukan perlakukan lebih lanjut dari target yang diharapkan 0.900 gr/ml, yaitu dengan melakukan penguapan air dalam oven pada suhu lebih tinggi dan durasi yang lebih panjang. Kata kunci: biodiesel, minyak jelantah, transesterifikasi, Fatty Acid Ethyl Ester (FAEE