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PERUBAHAN STRUKTUR HISTOLOGIS INSANG DAN HATI IKAN NILA (Oreochromis niloticus Linnaeus 1758) YANG TERPAPAR MERKURI Zulfahmi, Ilham; Affandi, Ridwan; Batu, Djamar T.F. Lumban
Jurnal Edukasi dan Sains Biologi Vol 4, No 1 (2015): Jurnal Edukasi dan Sains Biologi
Publisher : Universitas Almuslim

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Abstract

Penelitian ini bertujuan untuk mengkaji perubahan struktur histologis organ insang, hati dan ginjal ikan nila akibat dari paparan merkuri. Penelitian dilakukan dari bulan Februari hingga Juni 2013. Ikan nila berukuran panjang 11-13 cm dengan bobot rata-rata 20 gram dipaparkan pada konsentrasi sub kronik merkuri klorida (0,164 mgL-1) selama 56 hari. Pembuatan preparat histologis hati dan dilakukan dengan metode histoteknik menggunakan pewarnaan Haemotoxylin dan Eosin. Hasil penelitian menunjukkan adanya pengaruh negatif pada hati dan ginjal ikan nila akibat dari paparan merkuri. Paparan merkuri pada organ insang menyebabkan terjadinya perubahan struktur histologis berupa perbesaran sel organ (hypertrophy), penambahan jumlah sel (hyperplasia) pembengkokan lamela sekunder (curling of secondary lamella), penghimpitan lamella sekunder (fusion in secondary lamella)  dan kematian sel  (neukrosis). Perubahan histologis pada organ hati berupa perbesaran sel organ (hypertrophy), penambahan jumlah sel (hyperplasia), penciutan inti  sel (shrinkage of hepatocytes), pendarahan (hemorage), dan kematian sel (neukrosis).Kata kunci: merkuri klorida, histologis, hypertrophy, hyperplasia, neukrosis.
Distribusi Ukuran Spasial-Temporal dan Berdasarkan Tingkat Kematangan Gonad Rajungan (Portunus pelagicus Linnaeus 1758) di Teluk Lasongko, Buton Tengah, Sulawesi Tenggara Hamid, Abdul; Wardiatno, Yusli; Batu, Djamar T.F. Lumban; Riani, Etty
Journal Omni-Akuatika Vol 12, No 2 (2016): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

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The aim of the study was to reveal spatial-temporal distribution of the blue swimming crab(Portunus pelagicus Linnaeus 1758) in terms of size and gonad maturity stage of both male andfemale. Sampling of crab was conducted with a gill net from April 2013 until March 2014 inLasongko Bay. Of the whole population the mean size of male was smaller than that of females.Spatially smaller crabs (juveniles and young ones) inhabited mostly inner side of the bay, whilemedium size crabs occurred in the middle part of the bay. Big crabs were mostly found in theouter side of the bay. In terms of time, big sise of male and female crabs were collected inperiod of October to November and September to March, respectively. Small size of male andfemale occurred in February to September and in April, respectively. Related to gonad maturity,the sizes of both sexes with mature stages were variable. Ovigerous female (OF) was presencewidely in the bay, but the size of ovigerous females living in the shallow part of the bay wassmaller than those living in the deeper part. Keywords: blue swimming crab, size distribution, carapace width, spatial-temporal, gonadmaturity 
PERUBAHAN STRUKTUR HISTOLOGIS INSANG DAN HATI IKAN NILA (Oreochromis niloticus Linnaeus 1758) YANG TERPAPAR MERKURI Zulfahmi, Ilham; Affandi, Ridwan; Batu, Djamar T.F. Lumban
Jurnal Edukasi dan Sains Biologi Vol 4, No 1 (2015): Jurnal Edukasi dan Sains Biologi
Publisher : Universitas Almuslim

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Kondisi biometrik ikan nila, Oreochromis niloticus (Linnaeus 1758) yang terpapar merkuri [Biometric condition of nile tilapia, Oreochromis niloticus (Linnaeus 1758) after mercury exposure] Zulfahmi, Ilham; Affandi, Ridwan; Batu, Djamar T.F. Lumban
Jurnal Iktiologi Indonesia Vol 14, No 1 (2014): Februari 2014
Publisher : Masyarakat Iktiologi Indonesia (MII )

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The aims of this study are to examine the changes in biometric variables of nile tilapia caused by mercury exposure. The study was conducted from February to June 2013. Test fish were exposed to 0 mg L-1, 0.164 mg L-1, and 0.196 mg L-1 mercury chloride for 56 days. Analysis was done for the survival rate, growth rate, hepatosomatic index, the relative bile volume, gonadal somatic index, fecundity, and oocyte diameter. The median lethal concentration (96 hrs, LC50) of mercury chloride was calculated as 1.64 mg L-1. The survival rate was highest in the control treatment (46.67%). Mercury chloride with concentration 0.196 mg L-1 shows significant effect to changes HSI and relative bile volume, and oocyte diameter of Nile tilapia (p<0.05). Mercury chloride with a concentration of 0.164 mg L-1and 0.196 mg L-1 have not a significant effect on the growth rate of weight, gonadal somatic index, and fecundity of nile tilapia (p>0.05). AbstrakPenelitian ini bertujuan untuk mengkaji perubahan beberapa variabel biometrik ikan nila akibat dari paparan merkuri. Penelitian dilakukan dari bulan Februari hingga Juni 2013. Ikan nila berukuran panjang 11-13 cm dengan bobot rata-rata 20 gram dipaparkan pada tiga konsentrasi merkuri klorida (0 mg L-1; 0,164 mg L-1; dan 0,196 mg L-1) selama 56 hari. Analisis dilakukan terhadap tingkat kelangsungan hidup, laju pertumbuhan bobot, indeks hepatosomatik (HSI), volume empedu relatif, indeks kematangan gonad, fekunditas dan diameter telur. Hasil penelitian menunjukkan bahwa nilai LC50-96 jam merkuri klorida adalah sebesar 1,64 mg L-1. Tingkat kelangsungan hidup tertinggi terdapat pada perlakuan 0 mg L-1 (46,67%) dan terendah pada perlakuan 0,196 mg L-1 (40,00%). Merkuri klorida dengan konsentrasi 0,196 mg L"1 memberikan pengaruh yang nyata terhadap perubahan HSI dan volume empedu relatif serta ukuran diameter telur ikan nila (p<0,05). Namun pada konsentrasi 0,164 mg L-1dan 0,196 mg L-1 merkuri klorida tidak ber-pengaruh nyata terhadap laju pertumbuhan bobot, indeks kematangan gonad (IKG) dan fekunditas ikan nila (p>0,05).