Entris Sutisna, Entris
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Determination of Salbutamol and Guaifenesin in Mixture Using Zero- Crossing Wavelength Measurement

Indonesian Journal of Pharmaceutical Science and Technology Vol 2, No 2 (2015)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

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Abstract

A mixture of salbutamol and guaifenesin in syrup was analyzed using zero-crossing wavelength method. NaOH 0.1 N was selected as the solvent. Zero-crossing wavelength of salbutamol is 246.2 nm and guaifenesin is 270.2 nm. Results showed that the recovery of salbutamol and guaifenesin are 95.96% and 93.94%, respectively, while the coefficient of variance is 0.995% for salbutamol and 0.2087% for guaifenesin. Limit of detection of salbutamol and guaifenesin are 0.05528 ppm and 9.443 ppm, respectively, while limit of quantification are 0.18427 ppm and 31.477 ppm. We concluded that this method could be applied to determine salbutamol and guaifenesin in mixture. Keywords: Derivative spectrophotometry, guaifenesin, salbutamol, zero-crossing

Isolasi Dan Karakterisasi Asam Asiatat Dari Ekstrak Etanol Herba Pegagan (Centella Asiatica. (L.) Urban)

JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 4 (2015)
Publisher : Indonesian Research Gateway

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Abstract

Asam asiatat merupakan senyawa golongan triterpenoid pentasiklik yang terdapat dalam tanaman pegagan (Centella Asiatica. (L.) Urban). Penelitian ini bertujuan untuk mengisolasi senyawa asam asiatat dari ekstrak etanol herba pegagan. Isolasi dilakukan dengan metode maserasi dan teknik kromatografi, dan karakterisasi dilakukan dengan spektrofotometri UV Vis, IR, MS dan LC/MS-MS. Hasil isolasi berupa serbuk berwarna putih, dan memberikan serapan pada panjang gelombang maksimum (λmax) 206 nm, mempunyai gugus fungsi yang terdiri dari regang –OH (3433 cm-1), regang C-H alifatik (2929 cm-1), regang C=O (1709,82) dan ulur C-H (1462-1380 cm-1) serta ulur C-O (1242-1147 cm-1). Senyawa mempunyai massa molekul sebesar 489,4982. Isolat adalah asam asiatat yang mempunyai rumus molekul C30H48O5.