W Nurcahyo, W
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Detection of Toxoplasma gondii infection by Polymerase Chain Reaction (PCR) and Histological Examination on Balb/c Mice Hanafiah, M; Aliza, D; Rahmi, E; Nurcahyo, W
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Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

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Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
Toxoplasmosis Prevalence in Sheep in Daerah Istimewa Yogyakarta Nurcahyo, W; Prastowo, J; Sahara, A
ANIMAL PRODUCTION Vol 13, No 2 (2011): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. A research on toxoplasmosis prevalence in sheep was conducted in Daerah Istimewa Yogyakarta. The objective of the research was to understand the prevalence level of toxoplasmosis in sheep using skin test method by taking the membrane protein of tachyzoit produced in vivo. The research was initiated by producing the tachyzoit membrane protein at the testing animals, later the obtained protein was prepared and used in the skin test method. At the end of the research agglutination test was conducted to confirm the diagnosis using card agglutination test. An optimal dosage of tachyzoit membrane protein used in sheep as the basic material of the skin test was 1.5 mg/ml/head. Result showed the reaction of skin was thickening and the duration after being injected intradermally varied from 12 to 30 minutes in various sizes from 8 to 19 millimetres. The skin test method showed that the prevalence level of toxoplasmosis in Yogyakarta was more than 70%.Key Words: toxoplasmosis, prevalence, skin test