T Armansyah, T
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Biochemistry Characterization of Inhibin from Goat Granulosa Cells Siregar, TN; Aulanni’am, Aulanni’am; Susilawati, T; Riady, G; Hamdan, Hamdan; Armansyah, T
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective this research is to study the isolation and characterization of inhibin from goat granulosa cells.  Sample of ovary collected from abbatoir of Sukun in Malang. Minimum diameter of follicle that can be used in this research is 3 mm. Granulosa cells Oocytes collection was done by aspiration method, and granulosa cells separate from oosit mechanically. Biochemistry characterization of inhibin was done by technique of SDS PAGE, biuret and PAS. The result of research confirmed that inhibin has molecular 32 kDa. Composition of protein, carbohydrate, and glikoprotein inhibin was 3.636±0,002; 2.569±0,002; and 6.205±0.016 mg/mL, respectively. (Animal Production 8(2): 100-107 (2006) Key Words: Inhibin, granulosa cells, oocytes, follicle
The Efficacy of Seminal Vesicles Extract Administration on Percentage of Estrus and Pregnancy on Local Goat S, Syafruddin; Siregar, Tongku Nizwan; H, Herrialfian; Armansyah, T; Sayuti, Arman; R, Roslizawaty
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Syiah Kuala University

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The aim of this research was to determine the percentage of estrus, performance of estrus, and percentage of pregnancy in local goat which synchronized with seminal vesicles extract. The seminal vesicles used in this research were collected from the waste of Banda Aceh slaughter house. The goats were allotted into 2 groups. Goats in group were injected with 0.5 ml PGF2á intramuscularly, and goats in group II were injected with 5 ml seminal vesicles extract intrauterine. The injection was done twice with the interval of 11 days. The goats which perform estrus sign are mated naturally once in every observation. Pregnancy diagnosing was done using chemical urine method 2 months after mating. The estrus  percentage  and  pregnancy data were analyzed with chi-square  and  the estrus  performance (onset  and  estrus duration) were analyzed  using  student T test. The estrus onset of group I and II were 29.33±4.62 and 24.00±0.00 hours (P>0.05). Estrus duration of group I and II were 26.67+4.62 and 20.00+11.97 hours respectively (P>0.05). The  estrus percentage  of  group  I and  II did not  show  any significant differences (P>0.05), those are 60.0 and 40.0%  respectively,  whereas   the percentage of estrus  from  both  groups were 100.0 %.
UJI DAYA HAMBAT EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP PERTUMBUHAN Staphylococcus aureus (INHIBITORY TEST OF ETHANOL EXTRACT OF NONI LEAF (MORINDA CITRIFOLIA L.) ON STAPHYLOCOCCUS AUREUS GROWTH) Erina, Erina; Rinidar, Rinidar; Armansyah, T; Erwin, Erwin; Rusli, Rusli; Elsavira, Radhika
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 3 (2019): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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ABSTRAKMengkudu (Morinda citrifolia L.) merupakan tanaman khas daerah tropis termasuk Indonesia, yang buah, daun, biji dan akarnya sering digunakan dalam pengobatan tradisional, di antaranya sebagai antibakteri. Penelitian ini dilakukan bertujuan mengetahui kepekaan ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Staphylococcus aureus. Esktrak daun mengkudu dibagi menjadi 3 konsentrasi yang berbeda yaitu 25%, 50%, 75% dengan penicilin sebagai kontrol positif dan akuades sebagai kontrol negatif. Uji daya hambat dilakukan sesuai dengan metode Kirby-Bauer dengan 3 kali ulangan. Hasil rata-rata zona hambat ekstrak daun mengkudu terhadap bakteri Staphylococcus aureus pada konsentrasi 25% yaitu 6,35 mm, pada konsentrasi 50% yaitu 6,73 mm, dan pada konsentrasi 75% yaitu  6,86 mm. Kontrol positif antibiotik penicilin diperoleh rata-rata 14,28 mm dan kontrol negatif akuades diperoleh rata-rata 0,00 mm. Dapat disimpulkan bahwa ekstrak etanol daun mengkudu memiliki zona hambat sedang terhadap pertumbuhan Staphylococcus aureus sesuai dengan CLSI.Kata kunci: antibakteri, daya hambat, ekstrak daun mengkudu, Staphylococcus  aureus.   ABSTRACT Noni (Morinda citrifolia L.) is one of the most common plants in tropical areas, including Indonesia, which fruit, leaves, seed and root, are used in traditional treatment, for an example  antibacterial. The aims of this research is to know the sensitivity of noni leaf extract (Morinda citrifolia L.) against Staphylococcus aureus growth. Noni leaf extract was divided into 3 different concentrations 25%, 50%, 75%. Penicilin used as control positive and Aquadest used as control negative. The inhibition effect test was performed in accordance to Kirby-Bauer method with 3 replications. The average of inhibition zone of noni leaf extract on Staphylococcus aureus at 25% concentration was 6,35 mm, at concentration of 50% was 6,73 mm, and at concentration 75% was 6.86 mm. The averaged positive control of penicilin antibiotics was 14,28 mm and negative control of aquadest was 0,00 mm. It can be concluded that the ethanol extract of noni leaves had fair activity to inhibit the growth of Staphylococcus aureus according to CLSI.Keyword : antibacterial, inhibition effect, noni leaf extract, Staphylococcus aureus.