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STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
The Administration of Epididymis Extract Increased the Testosterone Concentration without Affects the Dihydrotestosterone Concentration in Local Male Goat Yuliansyah, Nanda; Akmal, Muslim; Siregar, Tongku Nizwan; Wahyuni, Sri; Abrar, Mahdi; Syafruddin, Syafruddin; Gholib, Gholib; Athaillah, Farida
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Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

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This study was aimed to determine the effect of epididymis extract (EE) on the testosterone and dihydrotestosterone (DHT) level of local male goat. An experimental study was performed using a completely randomized design (CRD) pattern of one-way analysis of variance (ANOVA). 15 local male goats aged 1.5 years with body weight 14-16 kg were used in this study. The K0 group as a control group, injected with only 1 ml physiological saline, while each KP1, KP2, KP3, and KP4 groups treated with multilevel EE dose, ie 1, 2, 3, and 4 ml / goat for 13 consecutive days. At the end of treatment (day 14th), testes, epididymis (caput, corpus, and cauda) and ductus deferens samples were taken through the close-castration method for examining the testosterone and DHT concentration by using enzyme-linked immunosorbent assay (ELISA) technique. Data gathered were later analyzed using ANOVA followed by Tukey’s HSD in SPSS 16.0 for Windows. The result showed that the average concentration of testosterone on K0, KP1, KP2, KP3, and KP 4 in testis respectively were 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. While the average concentration of DHT on K0, KP1, KP2, KP3, and KP 4 in testis respectively; 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. Statistical analysis showed that the administration of EE only increased testosterone concentration in testes had significant effect (P< 0.05). From this study, it can be concluded that the EE has the potential to improve spermatogenesis and sperm quality through increasing the testosterone concentration in the local male goats.
1. Immunohistochemical Study of the Immunoreactive Follicle Stimulating Hormone (ir-FSH) Cells Distribution in Pituitary Gland of Rat(Rattus norvegicus) Flaggellata, Patrick; Wahyuni, Sri; Jalaluddin, Muhammad; Hamny, Hamny; Gholib, Gholib; Armansyah, TR, T.; Akmal, Muslim
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The objective of this study was to identified the development of the immunoreactive follicle stimulating hormone (ir-FSH) cells in pituitary glands of rats (Rattus norvegicus)in different age using immunohistochemical (IHC) methods. The pituitary organ that used in this study was collected from eight white female rats aged 2, 4, 6, and >12 months. Pituitary glands were processed histologically and stained with IHC methods to detect the ir-FSH cells. The result showed that the ir-FSH cells were observed in pars distalis (PD), pars tuberalis (PT), and pars intermedia (PI) of adenohypophysis with different number of cells. Furthermore, ir-FSH cells were found abundantly (+++) in the PT and a few (+) in PD of rats aged two months. In rats aged 4 months those cells were distributed in slight number (+) in PT and abundant (+++) in PD. In addition, the cells were also found in a great quantities in rats whose ages were 6 months, (+++) in both PT and PD, but these cells decreased (++) in rats aged >12 months in PD and absent (-) in PT. In conclusion, the devalopment and distribution of ir-FSH cells were found in pars tuberalis and pars distalis adenohypophysis in the pituitary glands of rats aged 2 up to >12 months with different number and distribution patterns.
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Aulia, Rizki; Sugito, Sugito; Hasan, M.; Karmil, T. Fadrial; Gholib, Gholib; Rinidar, Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Abstract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P>0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella
3. The Effect of Audio Recording for Playback Experiment to the Alteration of Orangutan’s Territorial Behavior Nazif, Rahmat; Gholib, Gholib; Rahmi, Erdiansyah; Sayuti, Arman; Lubis, Triva Murtina; Balqis, Ummu
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The aim of this study was to find an alternative method to prevent the conflict between the orangutan and human with the utilization of audio recording as biofence. The location of this study was in Leuser National Park on Bukit Lawang, Langkat, North Sumatra using 16 orangutans as the subject of this study. The method used in this study was a simulation and playback experiment which were using long call, spontaneous call, predator voice, and siren sound in the audio recording of non-alpha orangutans. The respond was observed using binocular and recorded using camera recorder. The data obtained were analyzed using Krustal Wallist test. The result showed that 87% of orangutan showed an interest in the attractor, on the other hand, 13% showed disinterest to the attractor. The facial expression shown by orangutan was fear, submission, aggression, and worry. The statistical analyzing result showed that long call and the spontaneous call was very significant (P<0.01), while the predator and siren voice were insignificant (P>0.05). The result can be concluded that long call and spontaneous calls showing the effective result to the alteration of territorial behavior on orangutan and have a potential as a biofence that is used as an alternative method to prevent the conflict between orangutan and human.
STEROID LEVEL AND PREGNANCY RATE OF ACEH COWS IN RESPONSE TO OVULATION INDUCTION USING PRESYNCHOVSYNCH METHOD Adam, Mulyadi; Siregar, Tongku Nizwan; Wahyuni, Sri; Gholib, Gholib; Ramadhana, Cut Erika; Ananda, Riski; Afifuddin, Afifuddin
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mengetahui peningkatan level steroid dan persentase kebuntingan sapi aceh terhadap induksi ovulasi dengan metode presynch-ovsynch. Dalam penelitian ini digunakan sepuluh ekor sapi aceh betina dengan status tidak bunting, minimal dua bulan pascapartus, sudah pernah beranak, dan sehat secara klinis. Sapi dibagi atas dua kelompok, yang masing-masing terdiri atas lima ekor sapi. Kelompok pertama (K1) disinkronisasi berahi dengan metode presynch-ovsynch. Pada kelompok kedua (K2), disinkronisasi berahi menggunakan 5 ml PGF2α secara intramuskulus dengan pola penyuntikan ganda dengan interval 12 hari. Setelah  48 jam akhir perlakuan, sapi pada K1 dan K2 diinseminasi menggunakan semen beku fertil. Observasi berahi dilakukan setelah penyuntikan terakhir. Koleksi darah untuk pemeriksaan level estradiol dilakukan segera setelah inseminasi dilakukan sedangkan koleksi darah untuk pemeriksaan progesteron dilakukan pada hari ke-7 pasca-inseminasi. Level steroid diukur menggunakan teknik enzyme-linked immunosorbent assay (ELISA). Pemeriksaan kebuntingan dilakukan 90 hari pasca-inseminasi menggunakan teknik palpasi rektal. Seluruh sapi menunjukkan gejala berahi setelah perlakuan. Level estradiol dan progesteron pada K1 vs K2 masing-masing adalah 294,98±110,48 vs 392,76±11,6 pg/ml (P>0,05) dan 23,85±15,14vs 12,69±5,64ng/ml (P>0,05). Persentase kebuntingan pada K1 vs K2 masing-masing adalah 60,0 vs 0,0%. Dari hasil penelitian disimpulkan bahwa metode presynch-ovsynch tidak dapat meningkatkan level steroid tetapi dapat meningkatkan persentase kebuntingan pada sapi aceh.
REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib, Gholib; Agil, Muhammad; Supriatna, Iman; Purwantara, Bambang; Heistermann, Michael; Engelhardt, Antje
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P<0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P>0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.
PERBANDINGAN KONSENTRASI PROGESTERON SELAMA SIKLUS BIRAHI PADA DOMBA WARINGIN YANG DIINDUKSI PGF2α DAN KOMBINASI PGF2α DAN GnRH Rahayu, Yezi Gita; Siregar, Tongku Nizwan; Gholib, Gholib; Thasmi, Cut Nila; Herrialfian, Herrialfian; Daud, Razali; Zuhrawati, Zuhrawati; Hamdan, Hamdan; Rasmaidar, Rasmaidar
JURNAL ILMIAH PETERNAKAN TERPADU Vol 6, No 2 (2018): Juli 2018
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

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Abstract

This study aimed to compare the progesterone concentrations in Waringin sheep estrus cycle which induced by PGF2α or the combination of PGF2α and GnRH. This study used six Waringin sheep those were divided into twice groups, K1 and K2. K1(n=3) were injected by 7.5 mg PGF2α intramuscularly for 10 days, while K2 (n = 3) were injected by PGF2α and GnRH. On the first day K2 were injected by 7.5 mg PGF2α, then followed by injection 50 µg of GnRH on day 8th and re-injected by 7.5 mg PGF2α on day 15th. On day 18th, sample was re-injected using 50 µg GnRH. Waringin sheep those showed estrus symptoms were detected visually and with signs showed by sheep stud. Blood samples were taken on the 7th, 14th, and 21st day after the peak heat. The measurement of progesterone concentration was conducted by enzyme linked immunosorbent assay (ELISA). The results showed those the concentrations of progesterone on day 7th, 14th, and 21st on K1 vs K2 were 1.324±1.079, 7.607±8.922, and 5.220±1.653  vs 4.705±3.369, 4.184±5.512, and 1.797±0.898 ng/mL (P>0,05), respectively. In conclusion, the concentration of progesterone of Waringin sheep after inducing with PGF2α or combination PGF2α and GnRH at different cycle periode did not show differences. Keywords: Estrus Cycle, GnRH, PGF2α, Progesterone, Waringin Sheep
3. An Immunohistochemical Study of Alpha Estrogen Receptor (ERα) Development in Ovary and Uterus of Rat (Rattus norvegicus) Wahyuni, Sri; Desfariza, Chintya; Hamny, Hamny; Akmal, Muslim; Gholib, Gholib; Armansyah, T.
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This research aimed to observe and identify the development of estrogen receptor alpha (ERα) in the ovary and uterus of rats (Rattus norvegicus) at various age levels using immunohistochemistry (IHC). The ovary and uterus were collected from eight female rats aged 2, 4, 6, and > 12 months and processed histologically and stained with IHC staining. This research showed, the intensity of ERα expression was found in high abundance (+++) in stroma and the corpus luteum and was weakly found (+) in the theca cells, blood vessels, oocytes and germinal epithelium of rats aged 2 months. Additionally, ERα expression in rats aged 4 months had similarities with rats aged 2 months, but the intensity of ERα had increased (++) in ovarian theca cells, blood vessels, and oocytes. In ovaries of rats aged 6 and > 12 months, ERα expression had decreased (++) in the stroma and the corpus luteum and had a low expression (+) in the blood vessel and oocytes. The uterus of rats aged 2 months had low expression of ERα (+) in the stroma and luminal epithelium, moderate (++) in the myometrium, but was not found in the uterine glands. Furthermore, the expression in the uterus of rats at the age of 4 months had increased in the stroma and luminal epithelium (++) and uterine glands (+).  At the age of 6 months, it found excessively (+++) in the stroma, myometrium, and luminal epithelium, moderate expression (++) in the uterine glands.  The expression of ERα in uterus aged > 12 months was almost equal to the uterus of rats aged 6 months, but the expression in the myometrium and epithelial luminal had decreased (++). It can be concluded that the development and distribution pattern of ERα in the ovaries and uterus of rats are different at each age levels, it may be related to the growth and development of reproductive organs and also closely related to the estrus cycles of rats.
Concentration Of Cortisol Metabolites In Captive Sumatran Elephants At Elephant Conservation Facilities In Aceh Wahyuni, Rosa Rika; Wahyuni, Sri; Azhar, Al; Gholib, Gholib
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Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

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This study was conducted to validate the DRG Cortisol ELISA EIA-1887 Germany kit for measure the concentration of stress hormone metabolites (cortisol) from the feces and its correlation to the stressor factor in captive elephants in PKG and CRU of Aceh. These factors are location, diet and presence of livestock. There is no special treatment, observation based on the activity, behavior or natural condition of the animals. The sampling technique was non invasive, fresh dung samples of each (±20 gram) were collected from 25 elephants in CRU and PKG. Feces taken in the morning (before the animals are bathed) along with the observation of animal behavior. All samples were collected and stored at -200C until the analysis process. The validation test are analytic (parallelilmsm) and biological validation test. The analytic test result (paralillsm), showed that the sample curve was not parallel to the standard curve, but crossed the standard curve. While the results of biological validation test, DRG Cortisol ELISA EIA-1887 Germany kit can measure the concentration of cortisol hormone feces of Sumatran elephant and able to describe the difference of cortisol concentration relation to physiological events (stress vs non-stress). The mean values of cortisol metabolite concentrations from PKG Saree (Komplek PKG and Hutan Seunapet), Sampoiniet CRU, Cot Girek, Das Peusangan, Meulaboh and Aceh Timur were (577 ng/g and 400 ng/g), 435ng /g, 419ng /g, 517ng / g, 401ng/g and 425ng /g. The measurement results correlate with the physiological conditions and observed  factors.