Rosmaidar Rosmaidar, Rosmaidar
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The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae

Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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Abstract

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P<0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage

The Effect of Vitamin C Addition in Andromed® Dilution on Procentage of Motility and Spermatozoa Intact Plasma Membrane of Aceh Bull After Freezing

Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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This study aimed to determine the effect of vitamin C addition in andromed® dilution on procentage of motility and intact plasma membrane post thawing of aceh bull spermatozoa. This research used fresh semen aceh bull were collected using artificial vagina and divided into four treatments.Control treatment (P0) semen without the addition of vitamin C, first treatment (P1) semen plus vitamin C 0.5 g/100 ml of dilution, second treatment (P2) semen plus vitamin C 1.0 g/100 ml of dilution, third treatment (P3) semen plus vitamin C 2.0 g/100 ml of dilution. The experiment design used was completely randomized design (CRD). Data was analyzed using ANOVA (Analysis of Variant) and followed by Duncan’s multiple range (Duncan’s Multiple Range Test) 5% confidence interval. The result showed that the addition of vitamin C influence the motility and intact plasma membrane percentage of aceh bull spermatozoa after thawing. The addition of vitamin C 0.5 g/100 ml and 1.0 g/100 ml increase motility and intact plasma membrane percentage, while vitamin C 2.0 g/100 ml decrease motility and intact membrane plasma percentage after thawing.Key words: intact plasma membrane, motility, aceh bull, spermatozoa, vitamin C

AKTIVITAS ANTIBAKTERI EKSTRAK TEMPE TERHADAP BAKTERI Staphylococcus aureus ( Antibacterial Activity of Tempe Extracts on Staphylococcus aureus)

JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak tempe terhadap bakteri Staphylococcus aureus. Hasil penelitian ini bermanfaat untuk pengembanga tempe sebagai makanan fungsional. Tempe yang digunakan dalam penelitian ini adalah tempe komersial yang diperoleh dari pasar tradisional Darussalam, Banda Aceh. Proses ekstraksi tempe dilakukan menggunakan pelarut etil asetat dengan tingkat konsentrasi yang berbeda yaitu 25%, 50% dan 75%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi menggunakan kertas cakram pada media Mueller Hinton Agar (MHA), dan inkubasi dilakukan pada suhu 36-37˚C selama 24 jam. Hasil penelitian menunjukkan bahwa ektrak etil asetat tempe dengan konsentrasi 25%, 50% dan 75% memiliki daya hambat sebesar 6,6 mm, 6,7 mm dan 7,5 mm, sedangkan daya hambat kontrol positif menggunakan amoksisilin 31,6 mm. Aktivitas antibakteri yang dihasilkan ekstrak tempe termasuk dalam kategori sedang.(The aim of this research is to observe the antibacterial activity of tempe extract againts Staphlococcus aureus. The result of this research broadens the current knoledge of tempe as functional food. Samples of commercial tempe were obtained from a traditional market of Darussalam, Banda Aceh.Tempe samples were then extracted using ethyl acetate, each at three levels of concentration 25%, 50% and 75%. The antibacterial activity test of  tempe extracts was carried out using disc diffusion method on Mueller Hinton Agar (MHA), and incubation was conducted at 36-37˚C for 24 hours. The result showed that etil asetate extract of tempe withc concentration of 25%, 50% and 75% had inhibition zone of 6,6 mm 6,7 mm and 7,5 mm, meanwhile the used of amoxicilin (as a positive control) could inhibit Staphylococcus aureus growth with inhibition diameter was 31,6 mm. Antibacterial activities that produced asetat etil extract of tempe in middle category).

AKTIVITAS ANTELMINTIK BIJI Veitchia merrillii TERHADAP Ascaridia galli SECARA IN VITRO

Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

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Abstract

Tujuan penelitian ini adalah mengevaluasi aktivitas antelmintik ekstrak etanol biji Veitchia merrillii terhadap nematoda intestinal, Ascaridia galli. Metode fitokimia dilakukan untuk menguji keberadaan jenis kimiawi di dalam ekstrak. Efek dari ekstrak tersebut ditentukan melalui in vitro berdasarkan hambatan pergerakan dan kematian cacing. Sebanyak empat ekor cacing masing diperlakukan triplikat dalam phosphate buffered saline, 25 mg/mL, dan 75 mg/mL larutan ekstrak V. merrillii, dan 15 mg/mL albendazole. Pergerakan cacing diamati pada interval 9, 18, 27, dan 36 jam. Kematian cacing ditentukan berdasarkan ada tidaknya pergerakan badan, bagian kepala, dan ekor cacing. Kami menemukan bahwa larutan ekstrak V. merrillii mengandung tanin, alkaloid, flavonoid, triterpenoid, saponin, tetapi tidak mengandung steroid. Berdasarkan percobaan in vitro, penggunaan konsentrasi 25 dan 75 mg/mL ekstrak V. merrillii menunjukkan efek antelmintik. Ekstrak V. merrillii tersebut hanya efektif pada konsentrasi 75 mg/mL. Kajian tersebut mengindikasikan bahwa antelmintik berbasis herbal untuk mengendalikan A. galli berpontesi dikembangkan.

PENGARUH TINGKAT PAPARAN TIMBAL (Pb) TERHADAP PROFIL DARAH IKAN NILA (Oreochromis nilloticus) (The Influence Of The Level Of Lead (Pb) Exposure On The Blood Profiles Of Tilapia Fish (Oreochromis nilloticus))

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

Penelitian ini bertujuan mengetahui tingkat konsentrasi timbal yang berpengaruh terhadap profil darah ikan nila. Penelitian ini menggunakan 12 ekor ikan nila dengan kriteria sehat, bobot badan 15 – 18 gram, umur ± 2 bulan, jenis kelamin jantan. Penelitian ini menggunakan 4 kelompok perlakuan, P0 sebagai kontrol ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/L  dan pakan pelet, P2 diberikan paparan timbal 12,53 mg/L  dan pakan pelet dan P3 diberikan paparan timbal 25,06 mg/L  dan pakan pelet,  masing-masing perlakuan terdiri dari 3 ekor ikan nila. Perlakuan dilakukan selama 30 hari dan Penghitungan profil darah ikan dilakukan pada hari ke 31. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) pola searah. Pengambilan darah melalui vena caudalis dengan spuit 1 ml  sebanyak 0,5 ml, kemudian sampel darah ditempatkan pada tabung tube yang berisi EDTA. Darah diperiksa menggunakan hematology analyzer. Parameter yang diamati adalah jumlah leukosit total, jumlah eritrosit, kadar hemoglobin dan nilai hematokrit. Hasil penelitian menunjukkan rata-rata jumlah leukosit total P0 (148,20±64,54), P1 (139,03±44,81), P2 (101,67±4,46), dan P3 (111,90±0,85). Rata-rata jumlah eritrosit P0 (1,53±0,80), P1 (1,81±0,48), P2 (1,19±0,10), dan P3 (1,39±0,41). Rata-rata kadar hemoglobin P0 (6,13±3,10), P1 (8,53±1,46), P2 (5,00±0,36), dan P3 (5,93±2,02). Rata-rata nilai hematokrit P0 (23,90±15,37), P1 (24,47±14,38), P2 (21,60±2,94), dan P3 (23,70±9,49). Kesimpulan dari penelitian bahwa konsentrasi timbal 0 mg/L; 6,26 mg/L; 12,53 mg/L dan 25,06 mg/L yang dipaparkan selama 30 hari tidak mempengaruhi profil darah ikan nila.The aims of this research was to find out to determine the level of Lead (Pb) concentration that affect the blood profile of tilapia (Oreochromis nilloticus). This study used 12 tilapia with criteria healthy; body weight 15 – 18 gram; age ± 2 month; male. This study used 4 treatment groups, P0 as control, fish fed only pellets, P1 was given lead exposure 6,26 mg/L and pellet, P2 was given lead exposure 12,53 mg/L and pellet  and P3 was given lead exposure 25,06 mg/L and pellet. Each treatment consists of 3 tilapia. Treatment was done for 30 days and counted blood fish profile done on day 31. The design used was a complete randomized design (RAL) of unidirectional pattern. Blood taking through vena caudalis with 1 ml sryringe as much as 0,5 ml, then the blood sample is placed on a tube containing EDTA. Blood is examined using a hematology analyzer. Parameter observed were total leukocyte count, total erythrocytet, hemoglobin levels and hematocrit value. The results showed the average of total leukocyte count P0 (148,20±64,54), P1 (139,03±44,81), P2 (101,67±4,46), and P3 (111,90±0,85). Average of total erythrocytet P0 (1,53±0,80), P1 (1,81±0,48), P2 (1,19±0,10), and P3 (1,39±0,41). Average of hemoglobin levels P0 (6,13±3,10), P1 (8,53±1,46), P2 (5,00±0,36), and P3 (5,93±2,02). Average of hematocrit value P0 (23,90±15,37), P1 (24,47±14,38), P2 (21,60±2,94), and P3 (23,70±9,49).  The conclusion of research  showed that the concentration of lead 0 mg/L; 6,26 mg/L; 12,53 mg/L and 25,06 mg/L exposed  for 30 days  do not effect the blood profile of the tilapia. 

PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS USUS IKAN NILA (Oreochromis nilloticus) The Effect of Lead (Pb) Exposure to the Histopathology of Nile Tilapia (Oreochromis nilloticus) Intestine

JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 1 (2017): NOVEMBER - JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

Penelitian ini bertujuan mengetahui pengaruh paparan timbal (Pb) terhadap histopatologis usus ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat, bobot badan     15- 18 gram, umur ± 2 bulan, dan jenis kelamin jantan. Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain rancangan acak lengkap yang terdiri dari 4 kelompok perlakuan masing-masing dengan 3 ulangan. Semua kelompok diberikan pakan berupa pelet. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal 6,26 mg/L, P2 diberikan paparan timbal 12,53 mg/L, dan P3 diberikan paparan timbal 25,06 mg/L. Perlakuan dilakukan selama 30 hari, dan pengambilan organ usus dilakukan pada hari 31. Sampel usus kemudian diambil dan difiksasi dalam larutan fiksasi dilanjutkan dengan pembuatan sediaan histopatologis dan pewarnaan haematoksilin dan eosin (HE). Pengamatan histopatologis dilakukan dengan mikroskop cahaya biokuler, kemudian untuk pengambilan gambar dengan menggunakan fotomikrograf. Data yang diperoleh dianalisis secara deskriptif.  Hasil pemeriksaan histopatologis usus ditemukan edema, degenerasi lemak, nekrosis, erosi vili usus dan lisis vili usus. Dari hasil ini menunjukkan bahwa semakin tinggi konsentrasi timbal yang diberikan semakin parah kerusakan organ usus yang terjadi. Berdasarkan hasil penelitian dapat disimpulkan bahwa kosentrasi timbal 6,26 mg/l, 12,53 mg/l dan 25,06 mg/l dapat menyebabkan kerusakan jaringan secara histopatologis.The aims of this research was to find out to determine the effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) intestinal. This study used 12 tilapia fish with criteria: healthy, body weight 15-18 gram, age ± 2 month, and male sex. This study, is a laboratory experiment using complete randomized design with 4 treatments groups, each group were repeated 3 times. Each groups were fed with pellet. P0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/L and pellet feed, P2 is given lead exposure 12,53 mg/L and pellet feed and P1 is given lead exposure 25,06 mg/L. Treatment carried out for 30 days, and fish were  euthanized on 31 days. Intestinal samples were collected and fixed in fixation solution followed by histopathology preparation using haematoxylin and eosin (HE) staining. Histopathologic observations were performed with a biocular light microscope, then for shooting using photomicrograph. The data obtained were analyzed descriptively. Edema, fat degeneration, necrosis, erotion of intestine villi and lisis of intestine villi were found as the result of intestine histopathologic test. This result showed that higher lead concentration causes more severe intestine damage that occur. From this result can be concluded that 6,26 mg/l, 12,53mg/l and 25,06 mg/l causes tissue damage histopathologically.

PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS INSANG IKAN NILA (Oreochromis nilloticus). The Effect of Lead (Pb) Exposure to the Histopathology of Nile Tilapia (Oreochromis nilloticus) Gill

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

Penelitian ini bertujuan mengetahui pengaruh paparan timbal (Pb) terhadap histopatologis insang  ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat, bobot badan 15-18 gram, umur ± 2 bulan, jenis kelamin jantan. Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain rancangan acak lengkap yang terdiri atas 4 kelompok perlakuan masing-masing dengan 3 ulangan. Semua kelompok diberikan pakan pelet. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/l, P2 diberikan paparan timbal 12,53 mg/l, dan P3 diberikan paparan timbal 25,06 mg/l. Perlakuan dilakukan selama 30 hari, dan pengambilan organ insang dilakukan pada hari 31. Sampel insang kemudian diambil dan difiksasi dalam larutan Davidson 10% dilanjutkan dengan pembuatan sediaan histopatologis dan pewarnaan hematoksilin dan eosin (HE). Pengamatan histopatologis dilakukan dengan mikroskop cahaya biokuler, kemudian untuk pengambilan gambar dengan menggunakan fotomikrograf. Data yang diperoleh dianalisis secara deskriptif.  Hasil pemeriksaan histopatologis insang ditemukan edema, kongesti, nekrosis, hiperplasia lamela sekunder, dan fusi lamela. Dari hasil ini menunjukkan bahwa semakin tinggi kosentrasi timbal yang diberikan semakin parah kerusakan organ insang yang terjadi. Berdasarkan hasil penelitian dapat disimpulkan bahwa kosentrasi timbal dapat meningkatkan kerusakan jaringan secara histopatologis.The aims of this research was to determine The effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) gill. This study used 40 tilapia fish with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study, is a laboratory experiment (in vivo) using complete randomized design with 4 treatments groups, each group were repeated 3 times. Each groups were fed with pellet. P0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/L and pellet feed, P2 is given lead exposure 12,53 mg/L, P3 is given lead exposure 25,06 mg/L. Treatment carried out for 30 days, and fish were  euthanized on  the 31st days. Gill samples were then collected and fixed in Davidson 10% solution followed by histopathology preparation using haematoxylin and eosin (HE) staining. Histopathologic observations were performed using a biocular light microscope, then for using photomicrograph. The data obtained were analyzed descriptively. The results of gill histopathologic examination found edema, congestion, necrosis, primary and secondary lamellae hyperplasia, and lamellae fusion. The result showed that the higher lead concentration the more severe damage on fish gills.  Based on result of this research can be conclided that lead concertration can increased tissue demage histopathologically.

SURVEI PENGETAHUAN DAN SIKAP PEMILIK RUMAH MAKAN DI KOTA BANDA ACEH TERHADAP KEHALALAN OLAHAN PANGAN ASAL HEWAN (Survey of Knowledge and the Attitude of Restaurant Owners in Banda Aceh Againts Halal Food Animal Origin)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Penelitian ini bertujuan menilai pengetahuan dan sikap pemilik rumah makan terhadap kehalalan olahan pangan asal hewan di Kota Banda Aceh. Penelitian ini dilaksanakan pada bulan Januari 2017 di beberapa rumah makan di Banda Aceh. Penelitian dilakukan dalam bentuk survei lapangan dengan mewawancarai responden menggunakan kuesioner terstruktur. Responden dipilih secara proporsional terhadap pemilik rumah makan di Banda Aceh.  Kriteria responden adalah beragama Islam, berumur ≥20 tahun, pemilik rumah makan di lokasi penelitian, rumah makan menjual olahan daging ayam, dan rumah makan dengan kebutuhan daging ayam >20 ekor/hari. Data hasil penelitian dianalisis secara deskriptif dan dilanjutkan dengan uji korelasi. Hasil penelitian terhadap 45 responden menunjukkan bahwa persentase terbesar pengetahuan pemilik rumah makan terhadap kehalalan olahan pangan asal hewan di Kota Banda Aceh adalah sebesar 82,2%, yang memiliki kategori baik, sedangkan persentase terbesar dari sikap pemilik rumah makan terhadap kehalalan olahan pangan asal hewan di Kota Banda Aceh adalah sebesar 44,4%, yang memiliki kategori kurang. Hasil uji korelasi menunjukkan nilai koefisien korelasi sebesar 0,43 yang dapat dikategorikan memiliki hubungan sedang. The aim of this study was to assess the knowledge and the attitude of restaurant owners to halal food processed of animal’s origin in Banda Aceh. This study was conducted on January 2017 in several restaurants in Banda Aceh by interviewing respondents using a structured questionnaire. The Respondents were selected proportionally. The Criteria of respondents were Moeslim, aged ≥ 20 years, restaurant owners which were in the study site, and a restaurant processed chicken meat and meal demand >20 carcass /day. The data were analyzed descriptively by using correlation test. As much as 45 respondents had been interviewed. The results showed that the largest percentage of  knowledge of restaurant owners on the halal processed was 82,2%. The percentage of restaurant owner attitudes towards halal processed was less of 44.4%. So that, correlation value categorized as medium (0,43).Keyword : knowledge, attitude, restaurants owners, halal, animal foods origin

PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS HATI IKAN NILA (Oreochromis nilloticus) ( The effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) liver)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

ABSTRAK Penelitian ini bertujuan mengetahui pengaruh paparan timbal  terhadap histopatologis hati ikan nila (Oreochromis nilloticus).Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat; bobot badan 10 – 20 gram; umur ± 2 bulan; jenis kelamin jantan.Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) pola searah dengan 4 kelompok perlakuan dan 3 ulangan. Semua kelompok diberikan pakan pelet yang diberikan setiap hari. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal 6,26 mg/l, P2 diberikan paparan timbal 12,53 mg/l dan P3 diberikan paparan timbal 25,06 mg/l.Perlakuan dilakukan selama 30 hari dan ikan dietanasi pada hari ke-31. Hasil menunjukan rata-rata (± SD) jumlah sel-sel hepatosit hati pada ikan  yang mengalami degenerasi P0 (0,93 ± 0,61); P1 (18,73 ± 4,95); P2 (22,33 ± 4,57);dan P3 (44,80 ± 5,20); dan nekrosis P0 (0,73 ± 0,50); P1 (8,53 ± 2,50); P2 (8,93 ± 0,83); dan P3 (16,73 ± 2,00). Dari hasil penelitian dapat disimpulkan bahwa paparan timbal dengan dosis 25,06 mg/l menyebabkan kerusakan degenerasi dan nekrosis yang berat secara histopatologis pada sel hepatosit hati ikan nila (Oreochromis nilloticus).ABSTRACT The aims of this research was to find out to determine the level of lead (Pb) concentration that affect the rate of growth tilapia fish (Oreochromis nilloticus). This study used twelve tilapia fish with criteria: healthy; body weight 10-20 gram; age ± 2 month; male sex. The research was designedby a completely randomized design (CRD) with 4 unidirectional patterngroup treatments and three reins. All groups are given pellet feed given daily. GroupP0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/l, P2 is given lead exposure 12,53 mg/l and P3 is given lead exposure 25,06 mg/l. Treatment carried out for 30 days andfish were euthanized on day 31. The statistical analysis showed that the average (± SD) number of hepatocyte liver cells in fish with degeneration wereP0 (0.93 ± 0.61), P1 (18.73 ± 4.95), P2 (22.33 ± 4.57), and P3 (44.80 ± 5.20), and necrosis were P0 (0.73 ± 0.50), P1 (8.53 ± 2.50), P2 (8.93 ± 0.83), and P3 (16.73 ± 2.00). From the results of the study can be concluded lead exposure with dose 25,06 mg/l cause damage degeneration and necrosis which is heavily histopatological in hepatocytes cell of Nile tilapia (Oreochromis nilloticus) liver.

PENGARUH PAPARAN TIMBAL (Pb) TERHADAP LAJU PERTUMBUHAN IKAN NILA (Oreochromis nilloticus)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Abstract

 Penelitian ini bertujuan mengetahui tingkat konsentrasi timbal   (Pb)  yang berpengaruh terhadap laju pertumbuhan ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 40 ekor dengan kriteria: sehat; bobot badan 15 – 18 gram; umur ± 2 bulan; jenis kelamin jantan. Penelitian ini menggunakan 4 kelompok perlakuan, P0 sebagai kontrol ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/L  dan pakan pelet, P2 diberikan paparan timbal 12,53 mg/L  dan pakan pelet dan P3 diberikan paparan timbal 25,06 mg/L  dan pakan pelet,  masing-masing perlakuan terdiri dari 10 ekor ikan nila. Perlakuan dilakukan selama 30 hari dan Pengukuran pertumbuhan ikan dilakukan setiap 10 hari sekali dengan cara menimbang bobot dan mengukur panjang tubuh setiap individu ikan. Hasil penelitian menunjukkan rata-rata panjang tubuh ikan nila P0 (9,45±1,06), P1 (8,89±0,90), P2 (8,86±0,87), dan P3 (8,66±0,85). Rata-rata berat ikan nila P0 (23,38±4,50), P1 (19,75±2,27), P2 (19,15±2,10), dan P3 (18,65±2,00). Laju pertumbuhan spesifik P0 (38,7%), P1 (8,3%), P2 (4,3%), dan P3 (3,3%). Laju pertumbuhan panjang harian individu ikan P0 (0,46 mm/d), P1 (0,27 mm/d), P2 (0,21 mm/d), dan P3 (0,19 mm/d). Kesimpulan dari penelitian bahwa konsentrasi timbal  dan lamanya paparan berpengaruh terhadap laju pertumbuhan ikan.  Konsentrasi timbal  yang sangat berpengaruh adalah 25,06 mg/L. ABSTRACTThe aims of this research was to find out to determine the level of Lead (Pb) concentration that affect the rate of growth tilapia (Oreochromis nilloticus). This study used 40 tilapia with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study used 4 treatment groups, P0 as control, fish fed only pellets, P1 was given lead exposure 6,26 mg/L and pellet, P2 was given lead exposure 12,53 mg/L and pellet and P3 was given lead exposure 25,06 mg/L and pellet. Each treatment consisted of 10 tilapia fish. Treatment carried out for 30 days and measure every 10 days. The results showed the average length of tilapia fish P0 (9,45±1,06b); P1 (8,89±0,90a); P2 (8,86±0,87a) and P3 (8,66±0,85). Average weight of tilapia fish P0 (23,38±4,50); P1 (19,75±2,27); P2 (19,15±2,10) and P3 (18,65±2,00). Spesific growth rate P0 (38,7%); P1 (8,3%); P2 (4,3%) and P3 (3,3%). The rate of long-term growth of individual fish P0 (0,46 mm/d); P1 (0,27 mm/d); P2 (0,21 mm/d) and P3 (0,19 mm/d). In conclusion, it showed that the concentration of lead (Pb) and the duration of exposure has affect the growth rate of fish. The influential concentration of lead is 25,06 mg/L.