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Susceptibility test of Griseofulvin, Ketoconazole, Itraconazole, and Terbinafine to Dermatophyte Species Using Microdilution Method Anggarini, Dyah Ratri; Sukanto, Hari; Astari, Linda; Endraswari, Pepy Dwi
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikkk.V27.1.2015.55-62

Abstract

Backgroud: Dermatophytosis is common disease in tropical countries such as Indonesia. The prevalence of dermatophytosis in general population also high (20%). The dermatophyte fungi are the etiologic agents that cause this disease, some of them had already reported to be resistant to some anti-fungi. Purpose: To determine dermatophyte species causing dermatophytosis and the resistancy of griseofulvin, ketoconazole, itraconazole, dan terbinafine to dermatophyte species. Methods: Isolates of dermatophyte from patient who met the inclusion criteria in outpatient clinic of dermato-venereology Dr. Soetomo Hospital Surabaya during October until December 2014 were analyzed with respect to their susceptibility to four anti-fungal agents (griseofulvin, ketoconazole, itraconazole, and terbinafine) using microdilution methode. Results: Thirthy patients were included in inclusion criteria, with T. mentagrophytes var. Interdigitale was the most common cause of dermatophytoses. According to MIC all 30 isolates were already resistant to all anti-fungal agent, except T. rubrum that still sensitive to ketoconazole and 80,  itraconazole.  Conclusion: According to MIC 16.7% isolates sensitive to griseofulvin, 23.3% to ketoconazole, 16.7% to 80 itraconazole and 20% to terbinafine.Key word: dermatophyte species, susceptibility test of anti-fungal, microdilution.
ANTIBODY ANTI-DENGUE PROFILE IN DENGUE HEMORRHAGIC FEVER PATIENTS AT DR. SOETOMO HOSPITAL, SURABAYA Waskito, Langgeng A; Notopuro, Paulus B; Endraswari, Pepy Dwi
Folia Medica Indonesiana Vol 51, No 3 (2015): July - September 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v51i3.2830

Abstract

Dengue hemorrhagic fever is one infectious diseases that have high case fatality rate in Indonesia. The aim of this study was to descibe the antibody anti-dengue profile in dengue hemorrhagic fever patients at Dr. Soetomo Hospital Surabaya. This study was epidemiological descriptive study to aims prevalence primary and secondary infection of dengue hemorrhagic fever and its clinical appearances. This study used 85 samples by taking medical record data of IgM and IgG anti-dengue serologic test from Internal Medicine Department, Dr. Soetomo Hospital, Surabaya, between January to December 2010. The data were collected and analyzed in diagrams and tables. Result of this study showed 25 (29,4%) patients had positive IgM serologic test, 14 (16,4%) patients had positive IgG serologic test and 46 (54,2%) patients had positive IgM and IgG serologic test. In primary infections, the history of fever was 3.8±1.2 days; platelet counts 58,6±21,4/ml and bleeding sign 37% of 25 patients. In secondary infections, the history of fever was 4.28±1.15 days; platelet count was 44,17±24,2/ml and bleeding sign 52% of 60 patients. In conclusion, patients with secondary infection have higher prevalence than primary infection. Then in secondary infection had more severe clinical appearance than primary infection.
Perbandingan Efektivitas Antifungi Ekstrak Etanol Bawang Putih (Allium sativum) dan Nistatin dengan Metode Difusi Cakram terhadap Candida albicans Paramesti, Sarwendah; Munir, Ratna Sofaria; Endraswari, Pepy Dwi
Jurnal Mikologi Indonesia Vol 3, No 1 (2019): Juni 2019
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bawang putih (Allium sativum) merupakan obat yang sudah dimanfaatkan sejak zaman dahulu oleh Hippocrates, Pliny, dan Aristotle untuk kegunaan terapi. Bawang putih mempunyai efek sebagai antivirus, antibakteri, antifungi, dan antioksidan. Selain itu, bawang putih dapat digunakan sebagai anti-atherosklerosis dan anti-kanker (Bongiorno, 2008). Di sisi lain, nistatin merupakan antibiotik yang sangat efisien dalam pengobatan mikosis. Dalam sehari-hari, nistatin digunakan sebagai obat untuk mengatasi infeksi yang disebabkan oleh jamur genus Candida. Penyebab utama penyakit tersebut adalah sistem imun tubuh yang melemah akibat penyakit sistemik. Penyebab lainnya yaitu akibat adanya infeksi nosokomial yang ditularkan melalui tenaga medis mau pun alat-alat kesehatan yang telah terkontaminasi. Sejauh ini penelitian yang dilakukan sebelumnya untuk menentukan aktivitas antifungi yang dihasilkan oleh bawang putih saja, tanpa mengetahui perbandingan efektivitasnya dengan obat-obatan lain, sehinga perlu diadakan penelitian lebih lanjut mengenai hal tersebut. Tujuan penelitian ini adalah membandingkan efektivitas ekstrak etanol bawang putih (Allium sativum) dan nistatin sebagai antifungi yang dapat menghambat pertumbuhan jamur Candida albicans. Metode yang digunakan dalam penelitian adalah uji difusi menggunakan kertas cakram dengan 6 perlakuan. Konsentrasi ekstrak yang dipilih adalah 4g/ml (100%), 3g/ml (75%), 2g/ml (50%), dan 1g/ml (25%). Replikasi yang dilakukan dalam penelitian ini sebanyak 5 kali. Hasil data diperoleh dari pengukuran besar diameter zona hambat yang terbentuk di sekitar media perbenihan jamur Candida albicans yang sudah diinkubasikan pada suhu 37ºC selama 24 jam, dalam satuan milimeter. Kemudian, hasil tersebut akan dibandingkan dengan besar diameter zona hambat yang dihasilkan oleh obat nistatin untuk dinilai efektivitas keduanya. Hasil dari penelitian ini didapatkan bahwa besar diameter zona hambat yang dihasilkan oleh nistatin jauh lebih besar dibandingkan ekstrak etanol bawang putih (Allium sativum). Perlu dilakukan evaluasi baik dari segi tanaman, jamur, obat, cara ekstraksi mau pun metode penelitian agar dapat memberikan hasil yang lebih akurat.
Progress In Knowledge Of Microscopic Tuberculosis Diagnosis Laboratory Training Participants Sutandhio, Silvia; Mertaniasih, Ni Made; Koendhori, Eko Budi; Kusumaningrum, Deby; Sulistyowati, Titiek; Alimsardjono, Lindawati; Endraswari, Pepy Dwi; Wahyunitisari, Manik Retno; Setiabudi, Rebekah Juniati
JURNAL WIDYA MEDIKA Vol 5, No 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2202

Abstract

Indonesia is the second highest tuberculosis (TB)-burden country in the world. TB affects men and women in productive ages. Early and accurate diagnosis of TB is crucial in TB management because it allows prompt treatment with appropriate regimen. Sputum smear microscopy is the most common method used in resource-limited laboratories. The aim of this study is to assess whether adult learning method with student-centered approach can improve knowledge in laboratory techniques in microscopic TB diagnosis. It was a quasi experimental research with one group pretest and posttest design. Pretest was conducted before the training started. During the training, participants attended lectures, discussion sessions, and hands-on laboratory activities. After training, participants were asked to respond to posttest. There were 33 participants of this training (n=33). Paired t-test analysis showed significant difference between pretest and posttest scores (p=6.5x10-9). Variance between pretest scores was 4.00 and variance between posttest scores were 1.34. Pearson correlation coefficient was 0.45. Regardless their level of knowledge before training, participants could achieve relatively equivalent results after training. Significant difference between pre- and post-training test scores indicated that the learning method was effective and the training could improve participants knowledge in laboratory techniques in microscopic TB diagnosis.
Susceptibility test of Griseofulvin, Ketoconazole, Itraconazole, and Terbinafine to Dermatophyte Species Using Microdilution Method Anggarini, Dyah Ratri; Sukanto, Hari; Astari, Linda; Endraswari, Pepy Dwi
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Fakultas Kedokteran Universita Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikkk.V27.1.2015.55-62

Abstract

Backgroud: Dermatophytosis is common disease in tropical countries such as Indonesia. The prevalence of dermatophytosis in general population also high (20%). The dermatophyte fungi are the etiologic agents that cause this disease, some of them had already reported to be resistant to some anti-fungi. Purpose: To determine dermatophyte species causing dermatophytosis and the resistancy of griseofulvin, ketoconazole, itraconazole, dan terbinafine to dermatophyte species. Methods: Isolates of dermatophyte from patient who met the inclusion criteria in outpatient clinic of dermato-venereology Dr. Soetomo Hospital Surabaya during October until December 2014 were analyzed with respect to their susceptibility to four anti-fungal agents (griseofulvin, ketoconazole, itraconazole, and terbinafine) using microdilution methode. Results: Thirthy patients were included in inclusion criteria, with T. mentagrophytes var. Interdigitale was the most common cause of dermatophytoses. According to MIC all 30 isolates were already resistant to all anti-fungal agent, except T. rubrum that still sensitive to ketoconazole and 80,  itraconazole.  Conclusion: According to MIC 16.7% isolates sensitive to griseofulvin, 23.3% to ketoconazole, 16.7% to 80 itraconazole and 20% to terbinafine.Key word: dermatophyte species, susceptibility test of anti-fungal, microdilution.