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Hubungan Imunoekspresi Human Telomerase Reserve Transcriptase (hTERT) dan Vascular Endothelial Growth Factor (VEGF) dengan Gradasi Histopatologi Meningioma Marlina, Betty; Yantisetiasti, Anglita; Hassan, Abdul Hadi; Hernowo, Bethy S
JAMBI MEDICAL JOURNAL Vol 2, No 1 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACT Background: Meningioma is a tumor composed of cells of neoplastic meningothelial (arachnoidal) cells that a rise from the arachnoid membrane and the layer of the arachnoid villi associated with intradural venous sinuses and surrounding tissue. Meningiomas tumors is one of the central nervous system (CNS) the most commonly found (the second sequence) and generally grow slowly. Approximately 60-95% activity of telomerase (hTERT ) found on meningiomas and can induces VEGF . The aim of this study is to determine the role of hTERT and VEGF in meningiomas grading . Methods : This study is conducted using analytic observasional cross sectional method and had been analyzed with  unpaired categorical analysis of the 60 cases of meningioma (46 cases grade I, 7 cases grade II, and7 cases grade III). The sample were obtained the archive of Anatomical Pathology Department, Faculty of Medicine, Padjadjaran University/Dr. Hasan Sadikin Hospital Result : The result of this study showed: 44 cases (96%) grade I meningiomas showed positive hTERT imunoexpression weak to strong, 2 cases(4%) non-reactive and VEGF imunoexpression showed strong positive 12 cases (26%), 24 cases(52%) were moderate positive and 10 cases (22%) weak positive. 7 cases (100%) grade II showed a strong positive hTERT imunoexpression and 5 cases (71%) showed a strong positive VEGF imunoexpression, 2 cases (29%) were moderate positive. 6 cases (86%) grade III showed strong positive hTERT imunoexpression, 1 case (14%) were moderate positive and 7 cases(100%) showed a strong positive VEGF imunoexpression. Statistical analysis revealed imunoexpression VEGF showed stronger positivity with p- value = 0.02 (< 0.05) compared to the hTERT imunoexpression with p-value =  0.73 (> 0.05). Conclusion : More affecting angiogenesis gradations meningiomas, seen by the presence of VEGF higher imunoexpression on higher gradations. hTERT Imunoexpression cannot determine of the meningiomas histopathological grading.
Korelasi Ekspresi VEGF dan Densitas Mikrovesel Dengan Respons Radioterapi Adenokarsinoma Serviks Wibisono, Fennisia; Suryanti, Sri; Hernowo, Bethy S
JAMBI MEDICAL JOURNAL Vol 2, No 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACT Background: Most of cervical carcinoma patients, including cervical adenocarcinoma comes at an advanced stage and thus radiotherapy is one of the modality treatment. The success of radiotherapy depends on several prognostic factors such as the performance, staging, histopathology of tumor, presence of organ dysfunction and cellular biology marker such as VEGF. Activation singaling pathway Vascular Endothelial Growth Factor (VEGF) can induce radioresistance on cervical adenocarcinoma. Activation angiogenesis mediated by VEGF can be counted from microvessel density (MVD). Counting MVD use imunohistochemical CD34 on endothelial cells form capiller of blood vessel in peritumoral. The aim of this study was to determine correlation between immunohistochemistry VEGF dan MVD with neoadjuvant radiotherapy response cervical adenocarcinoma. Methods: This study was an observational study, cross-sectional and correlational analysis. Samples was collected by total sampling with amount 32 samples block parafin from Departement Anatomical Pathology General Hospital Dr. Hasan Sadikin Bandung and treated with complete neoadjuvant radiotherapy from Januari 2007 until December 2013. Immunohistochemical examination of VEGF and CD34 for counting MVD was done on all sample block parafin. Result: There was a strong correlation between microvessel density (MVD) with neoadjuvant radiotherapy response in cervical adenocarcinoma (p = 0.000). In this study MVD above 30 correlate with poor neoadjuvant radiotherapy response in cervical adenocarcinoma. Conclusion: Examination MVD recommended for cervical adenocarcinoma patient before radiation treatment.   Keyword : Cervical Adenocarcinoma, CD34, Neoadjuvant Radiotherapy, VEGF
Perbandingan Imunoekspresi p53 dan Caspase-3 Pada Mola Hidatidosa Parsial, Mola Hidatidosa Komplit, dan Koriokarsinoma Reis, Odilia Dos; Agustina, Hasrayati; Hassan, Abdul Hadi; Hernowo, Bethy S
JAMBI MEDICAL JOURNAL Vol 2, No 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

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ABSTRACT Background: Partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma are gestational trophoblastic diseases which have high incidence in Indonesia. Most complete hydatidiform mole can transform into choriocarcinoma , but pathogenesis of the transformation  is remain unknown. Apoptosis plays an important role in the pathogenesis of human malignancy. p53 and caspase - 3 play a role in apotosis . The aim of this study is to evaluate the p53 and caspase-3  immunoexpression in partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma Methods: This is an observational analytic cross  sectional  study, conducted on 30 cases of gestational trophoblastic diseases consisted of 10 cases of partial hydatidiform mole, 10 cases of complete hydatidiform mole and 10 cases of choriocarcinoma. Immunohistochemical  examination of p53 and Caspase-3 were  performed on all cases. Result : The result showed significant difference between the p53 immunoexpression in partial hydatidiform mole and choriocarcinoma (p = 0.002), there was no significant p53 immunoexpression difference between complete hydatidiform  mole and choriocarcinoma (p = 0.121), there was no caspase-3 immunoexpression difference between partial hydatidiform mole and choriocarcinoma (p = 0.160) and there was no caspase-3 immunoexpression difference between complete hydatidiform mole and choriocarcinoma  (p = 0.361). p53 Immunoexpresion was higher in the case of complete hydatidiform mole and choriocarcinoma but tend to be lower in partial hydatidiform mole. Conclusion : In conclusion, p53 may play a role in the pathogenesis of complete hydatidiform mole and choriocarcinoma, but has a lesser role in the pathogenesis of partial hydatidiform mole. Caspase-3 might not play a role in the pathogenesis of hydatidiform mole transformation into choriocarcinoma. Keywords: complete hydatidiform mole, partial hydatidiform mole, choriocarcinoma, p53, caspase-3.
Pendeteksian Ekspresi Biomarker MNK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Kisnanto, Teja; Wardani, Rina Tri; Siregar, Budiningsih; Amir, Mellova; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; Andrijono, A; Hernowo, Bethy S; Tobing, Maringan DL; Tetriana, Devita
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positif. Cervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.
Multiple human papilloma virus infections predominant in squamous cell cervical carcinoma in Bandung Sahiratmadja, Edhyana; Tobing, Maringan D.L; Dewayani, Birgitta M; Hernowo, Bethy S; Susanto, Herman
Universa Medicina Vol 33, No 1 (2014)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.58-64

Abstract

BACKGROUND Persistent infection of high risk genotypes of human papilloma virus (hrHPV) has been established as the etiological cause for cervical cancer, and the most prevalent genotypes that infect the cervical tissue are HPV-16 and HPV-18. However, HPV genotype profile has been shown to differ according to geographical distribution across the globe. The present study aimed to determine the HPV genotype distribution in cervical cancer patients from Bandung, Indonesia. METHODS During the period of July – November 2010 viral DNA was extracted from randomly chosen cervical cancer biopsies and subjected to genotype determination using the diagnostic linear array genotyping test (Roche). The distribution of HPV genotypes was explored and the prevalence of HPV genotypes was mapped. RESULTS Of 96 cervical cancer tissue samples, 76 (79.2%) were histopathologically classified as squamous cell cervical carcinoma. Due to the high cost of HPV genotyping tests, only twenty-five samples were randomly genotyped. Almost 90% of the cervical cancer patients were multiply infected with HPV-16 in combination with HPV-18, HPV-45, or HPV-52. The HPV-16 genotype had the highest prevalence, all samples being infected with HPV-16. CONCLUSION The cervical cancer cases were predominantly infected by multiple hrHPVs with HPV-16 as the major genotype among other hrHPVs, supporting the carcinogenic role of this hrHPV. Therefore, screening for hrHPVs in the general population is urgently needed as a means of early detection of cervical cancer.
ANALYSIS OF IMMUNOEXPRESSION OF ESTROGEN RECEPTOR BETA AND EXTRACELLULAR MATRIX METALLOPROTEINASE INDUCER (EMMPRIN) ON TESTICULAR SEMINOMAS NONRECURRENCE AND RECURRENCE Ridwan, Antonius Janes; Suryanti, Sri; Hassan, Abdul Hadi; Hernowo, Bethy S
Journal of Medicine and Health Vol 1, No 2 (2015)
Publisher : Maranatha Christian University

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Abstract

The most common malignancy of testis is seminoma with prognosis that is not always good because of high recurrence rate and death cases. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a glycoprotein adhesion molecules which plays a role in tumor cell invasion and metastasis as evidenced by immunohistochemistry method in several types of tumor cells and tissue that shows the correlation to the clinical progessivity.This study was conducted by observational descriptive analytics with each seven cases of seminoma nonrecurrences and recurrences diagnosed histopathologically from Dr.Hasan Sadikin Hospital, Advent Hospital and St.Borromeus Hospital in Bandung. This study shows that immunoexpression of estrogen receptor beta is not higher in testicular seminoma recurrence compared with nonreccurence. There was no higher imunoexpression of EMMPRIN receptor on testicular seminoma recurrence compared with nonrecurrence; besides, there was no relationship between immunoexspression of estrogen receptor beta and EMMPRIN in testicular seminoma nonrecurrence and recurrence.In conclusion, immunoexpression estrogen receptor beta expressed in all testicular seminoma, so that the patient could be given an alternative therapy and immunoexspression EMMPRIN, cannot be used to predict a recurrence in patients with seminoma who has received complete therapy.                                                        Keywords: EMMPRIN, Estrogen Receptor Beta, Seminoma Testis nonrecurrence and recurrence
Perbandingan Imunoekspresi p53 dan Caspase-3 Pada Mola Hidatidosa Parsial, Mola Hidatidosa Komplit, dan Koriokarsinoma Reis, Odilia Dos; Agustina, Hasrayati; Hassan, Abdul Hadi; Hernowo, Bethy S
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol 2 No 2 (2014): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jmj.v2i2.2713

Abstract

ABSTRACT Background: Partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma are gestational trophoblastic diseases which have high incidence in Indonesia. Most complete hydatidiform mole can transform into choriocarcinoma , but pathogenesis of the transformation  is remain unknown. Apoptosis plays an important role in the pathogenesis of human malignancy. p53 and caspase - 3 play a role in apotosis . The aim of this study is to evaluate the p53 and caspase-3  immunoexpression in partial hydatidiform mole, complete hydatidiform mole and choriocarcinoma Methods: This is an observational analytic cross  sectional  study, conducted on 30 cases of gestational trophoblastic diseases consisted of 10 cases of partial hydatidiform mole, 10 cases of complete hydatidiform mole and 10 cases of choriocarcinoma. Immunohistochemical  examination of p53 and Caspase-3 were  performed on all cases. Result : The result showed significant difference between the p53 immunoexpression in partial hydatidiform mole and choriocarcinoma (p = 0.002), there was no significant p53 immunoexpression difference between complete hydatidiform  mole and choriocarcinoma (p = 0.121), there was no caspase-3 immunoexpression difference between partial hydatidiform mole and choriocarcinoma (p = 0.160) and there was no caspase-3 immunoexpression difference between complete hydatidiform mole and choriocarcinoma  (p = 0.361). p53 Immunoexpresion was higher in the case of complete hydatidiform mole and choriocarcinoma but tend to be lower in partial hydatidiform mole. Conclusion : In conclusion, p53 may play a role in the pathogenesis of complete hydatidiform mole and choriocarcinoma, but has a lesser role in the pathogenesis of partial hydatidiform mole. Caspase-3 might not play a role in the pathogenesis of hydatidiform mole transformation into choriocarcinoma. Keywords: complete hydatidiform mole, partial hydatidiform mole, choriocarcinoma, p53, caspase-3.
Peran Konseling Laktasi dengan Penerapan Media terhadap Tingkat Keyakinan Diri dan Keberhasilan Menyusui pada Ibu Post partum Suksesty, Catur E; Hernowo, Bethy S; Damayanti, Meita; Husin, Farid; Sekarwana, Nanan
Jurnal Pendidikan dan Pelayanan Kebidanan Indonesia (Indonesian Journal of Education and Midwifery Care Vol 3, No 2 (2016): Juni
Publisher : Program Studi Magister Kebidanan FK UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.527 KB) | DOI: 10.24198/ijemc.v3i2.36

Abstract

Pemberian Air Susu Ibu (ASI) yang dilakukan sejak usia dini dan dilanjutkan dengan ASI eksklusif selama 6 bulan dapat menurunkan angka kesakitan dan kematian bayi, serta meningkatkan tumbuh kembang bayi secara optimal. Namun, secara global masih sedikit bayi di  bawah usia enam bulan yang diberikan ASI secara eksklusif, yaitu sebesar 40%. Beberapa studi telah dilakukan tentang pentingnya ASI, tetapi angka pemberian ASI belum memuaskan. Salah satu penyebab rendahnya pemberian ASI, yaitu karena ketidaktahuan ibu tentang proses laktasi yang menyebabkan kurangnya keyakinan ibu untuk dapat menyusui bayinya. Ibu memerlukan informasi yang tepat dari tenaga kesehatan tentang proses laktasi. Informasi tersebut disampaikan dalam proses konseling laktasi dan wajib ibu ketahui mulai dari masa kehamilan. Tujuan penelitian ini adalah untuk menganalisis pengaruh penggunaan media dalam konseling laktasi terhadap keyakinan diri dan keberhasilan menyusui ibu post partum. Penelitian ini merupakan penelitian kuasi eksperimen dengan rancangan post test only with control group design. Pengambilan sampel dengan cara consecutive sampling sesuai kriteria inklusi, yaitu sebesar 30 ibu primigravida usia kehamilan 36 minggu sampai 1 bulan post partum. Instrumen yang digunakan, yaitu Breastfeeding Self-Efficacy Scale Form (BSESF) dan lembar observasi kemampuan menyusui sesuai dengan modul konseling menyusui 40 jam WHO/UNICEF tahun 2011. Rancangan analisis penelitian ini menggunakan uji T dan uji Mann-Whitney serta uji Rank Spearman untuk mencari korelasi dengan bantuan SPSS. Keyakinan diri pada  usia kehamilan 36 minggu sampai 1 minggu post partum didapatkan nilai p<0,05, sedangkan pada waktu 1 hari post partum sampai 1 minggu post partum didapatkan nilai p<0,01. Tidak terdapat korelasi keyakinan diri terhadap kemampuan menyusui dan keberhasilan menyusui pada ibu post partum dengan nilap p>0,05.
Korelasi antara Imunoekspresi LMP-1 Virus Epstein-Barr dengan Respon Kemoterapi CHOP pada Limfoma Maligna Non-Hodgkin Tipe Diffuse Large B Cell. Susanti, Inas; Agustina, Hasrayati; Hernowo, Bethy S
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Diffuse large B cell lymphoma is the most common type of B-cell non-Hodgkin malignant lymphoma. Epstein-Barr virus (EBV) has been shown to contribute to the development of this tumor especially in the elderly patient (above 50 years). The chemotherapeutic agents CHOP (Cyclophospamide, Hydroxydaunorubicin, Oncovin, Prednison) are standard treatment for non-Hodgkin lymphoma patients, but approximately 30% cases shows non-respone with standard chemotherapy and undergoes progressive disease until death. There are no accurate predictor factors in predicting CHOP chemotherapy respone in patients with DLBCL. The aim of this study is to determine the role of LMP-1 in predicting CHOP chemotherapy respone in patients with DLBCL. Methods This study was done retrospectively in 34 cases of DLBCL. The samples were obtained from the Anatomic Pathology Department of Dr. Hasan Sadikin Hospital from January 2009 to Desember 2011. All cases were reviewed for histopathological diagnoses based on the WHO Classification of 2001 and the specimens were stained with specific antibodies against CD 20 (for diagnoses DLBCL) and LMP-1. The data were analized using rank Spearman’s correlation test. Results This study showed that 31 cases (91.18%) were LMP-1 positive, only 3 cases (8.82%) were negative. Three cases of negative LMP-1 had a good respone to chemotherapy, whereas among 31 positive cases LMP-1 showed good respone to chemotherapy in 21 cases and the remaining 10 cases had a poor respone to chemotherapy. There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL (p=0.436). Conclusion There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL. Key words: CHOP, DLBCL, EBV, LMP-1, non-Hodgkin lymphoma.
Pemeriksaan Real Time-Polymerase Chain Reaction (RT-PCR) pada Granuloma Well Organized dan Poorly Organized Limfadenitis Tuberkulosis Lestari, Dianti; Dewayani, Birgitta M; Hassan, Abdul Hadi; Hernowo, Bethy S
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Introduction Tuberculous lymphadenitis (TBL) was the most common form of extrapulmonary tuberculosis. The diagnose of TBL was based on the tubercle formation that consist of granuloma and caseous necrosis. There were 2 type of granuloma, well organized (WOG) and poorly organized granuloma (POG). Well organized granuloma were characterized by varying amount of eosinophilic necrosis surrounded by granuloma composed of mature epithelioid macrophages, mainly Langhans type giant cells and a mantle of lymphocytes and fibrous tissues. Poorly organized granuloma were characterized by central area of sparse coarse necrosis with nuclear debris and often polymorphonuclear granulocytes, the granuloma had an ill-defined mantle with mixed cells composed of macrophages, lymphocytes and plasma cells, only few giant cells were seen and there was little or no fibrosis. Inflamatory granuloma also can be found in non TBL, especially POG. The purpose of this study was to find out the etiology of WOG and POG based on the RT-PCR result. Methods This study used 30 formalin-fixed paraffin-embedded tissue blocks from patients who were histopathologically diagnosed as TBL with hematoxillin eosin (HE) staining, consist of 15 samples WOG and 15 samples POG. This study performed RT-PCR to all cases in order to find out the etiology of GWO and GPO and to know if there will be any differences in RT-PCR result between WOG and POG. Results Of the 15 WOG cases, 10 (67%) cases were RT-PCR positive and 5 (33%) cases were RT-PCR negative, whereas 15 cases POG consist of 8 (53%) cases were RT-PCR positive and 7 (47%) cases were RT-PCR negative. Both of cases WOG and POG was analyzed, according to the statistical analysis had found that there were no differences RT-PCR result between WOG and POG significantly (p>0.05). Conclusion There were no differences in RT-PCR result between histopathologic feature of WOG and POG. Key words : Poorly organized granuloma, RT-PCR, tuberculosis lymphadenitis, well organized granuloma.