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Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azhari, Azmi; Falah, Syamsul; Nurjannah, Laita; Suryani, Suryani; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.043 KB)

Abstract

Delignification is a lignin degradation, a preliminary process in industries that used cellulose containingsubstrates. Sengon logs are often used for the material in pulp industry because it has high levels of celluloseand low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. Themethods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, therate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemicalassay of sengon logs before and after delignification. The results of this study showed that delignification byT.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor).The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by37.31 % within 20 days. Chemical assay performed on delignified sengon wood showed decreased level ofethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicelluloselevel.
Trametes versicolor as Agent for Delignification of Rice Husks Nurjanah, Laita; Falah, Syamsul; Azhari, Azmi; Suryani, Suryani; Artika, I Made
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.043 KB)

Abstract

the earth for the production of biofuels and other valuable products. It is comprised of the carbohydratepolymers, cellulose, hemicellulose, and an aromatic polymer, lignin. One of the methods for removing thelignin component of rice husks is by delignification using white-rot-fungi. The aim of the study was to carry outdelignification of rice husks using white-rot-fungi. The white-rot-fungi used here were Trametes versicolor andPhanerochaete chrysosporium. The study consisted of a biomass and microbial preparation, chemical assay ofthe rice husk, ligninase enzyme tests, and delignification of rice husks. Results showed that T. versicolor and P.chrysosporium have ligninase enzyme. The precentage of lignin from the total biomass rice husks was 23.61%w/w, and following the delignification process by T. versicolor for 20 days, the remaining lignin was 16.20%w/w, making the percentage of rice husks lignin degraded as 7.41% w/w. The biodelignification process alsodecreased the percentage of holocellullose, cellulose, and other extracted substances, and accordingly thisincreased the percentage of hemicellulose. Based on the ability of T. versicolor to degrade lignin of the ricehusk at room temperature (28ºC) as mentioned above, it can be concluded that T. versicolor has potential tobe used for delignification process
AKTIVITAS SITOTOKSIK SENYAWA METABOLIT SEKUNDER KAPANG ENDOFIT Evodia suaveolens DENGAN METODE BSLT (Brine Shrimp Lethality Test) Azhari, Azmi
Scientiae Educatia: Jurnal Pendidikan Sains Vol 3, No 2 (2014)
Publisher : Tadris IPA Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.59 KB) | DOI: 10.24235/sc.educatia.v3i2.539

Abstract

Kanker adalah penyakit degeneratif dengan kasus yang makin meningkat setiap tahunnya. Menurut WHO (World Health Organization) (2003)  kasus kanker dunia diprediksi akan mengalami peningkatan 50%  menjadi 15 juta kasus pada tahun 2020.  Oleh sebab itu, diperlukan obat antikanker yang mencukupi kebutuhan dan juga efektif. Evodia suaveolens atau Zodia adalah tanaman khas Indonesia timur yang diketahuimengandung suatu senyawaantikakner yaitu evodiamin. Evodiamin yang dihasilkan dari Zodia tidaklah banyak sehingga diperlukan inovasi baru.Pemanfaatan kapang endofit yang diisolasi dari Evodia suaveolens diharapkan dapat menjadi solusi produksi Evodiamin yang kurang efektif dari tanamannya langsung. Produksi senyawa dengan mikroorganisme akan lebih cepat daripada produksi dengan organisme multiseluler dan potensi industri skala massalnya terbuka. Kapang endofit yang tumbuh dari suatu jaringan tanaman akan lebih dari satu jenis kapang, maka perlu ada uji screening awal untuk memilih kapang terbaik, salah satunya adalah denga metode BSLT. Isolasi kapang endofit dari batang Evodia suaveolens menghasilkan 8 isolat. Nilai LC50 dari masing-masing kapang endofit adalah  kapang 1 memiliki nilai 315 ppm, kapang 3 memiliki nilai 270 ppm, kapang 5 memiliki nilai 400 ppm, kapang 7 memiliki nilai 19.7 ppm dan kapang 8 memiliki nilai 403 ppm. Nilai ini membuktikan konsentrasi tersebut dapat membunuh populasi Artemia salina sebanyak 50%.
Front & Back Matter Volume 7 (2): June 2018 Azhari, Azmi
Scientiae Educatia: Jurnal Pendidikan Sains Vol 7, No 1 (2018): June (2018)
Publisher : Tadris IPA Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.021 KB) | DOI: 10.24235/sc.educatia.v7i2.4038

Abstract

Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azhari, Azmi; Falah, Syamsul; Nurjannah, Laita; Suryani, Suryani; Bintang, Maria
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.125 KB) | DOI: 10.29244/1-10

Abstract

Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.
Trametes versicolor as Agent for Delignification of Rice Husks Nurjanah, Laita; Falah, Syamsul; Azhari, Azmi; Suryani, Suryani; Artika, I Made
Current Biochemistry Vol 1, No 1 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.338 KB) | DOI: 10.29244/37-44

Abstract

Rice husks contains 33.71% w/w lignocelluloses, the most abundantly available raw material on the earth for the production of biofuels and other valuable products. It is comprised of the carbohydrate polymers, cellulose, hemicellulose, and an aromatic polymer, lignin. One of the methods for removing the lignin component of rice husks is by delignification using white-rot-fungi. The aim of the study was to carry out delignification of rice husks using white-rot-fungi. The white-rot-fungi used here were Trametes versicolor and Phanerochaete chrysosporium. The study consisted of a biomass and microbial preparation, chemical assay of the rice husk, ligninase enzyme tests, and delignification of rice husks. Results showed that T. versicolor and P. chrysosporium have ligninase enzyme. The precentage of lignin from the total biomass rice husks was 23.61% w/w, and following the delignification process by T. versicolor for 20 days, the remaining lignin was 16.20% w/w, making the percentage of rice husks lignin degraded as 7.41% w/w. The biodelignification process also decreased the percentage of holocellullose, cellulose, and other extracted substances, and accordingly this increased the percentage of hemicellulose. Based on the ability of T. versicolor to degrade lignin of the rice husk at room temperature (28 ºC) as mentioned above, it can be concluded that T. versicolor has potential to be used for delignification process.
PROTEOMIK: DATABASE DAN TEKNOLOGI Azhari, Azmi
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 8, No 1 (2017): April 2017
Publisher : Universitas Lambung Mangkurat

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Abstract

Abstract. Today the field of proteomics become one of the scientific disciplines that the spotlight of science. The integration of proteomics and bioinformatics studies linking between the database and technology. Databases are very helpful in the field of proteomics research. Database integrated into the proteomics experiment, aimed at determining the identity, characteristics, and interactions of proteins. An application form The GDPE is a web-based application with a relational database based PRIDE XML format. Protein sequence database provides peptide sequences that will be matched with tandem mass spectrum by search engines. Proteomic study the use of advanced tools called MALDI stands for Matrix-Assisted Laser Desorption/Ionization, with this tool will be known about the structure and function of a protein. The technique used in the study of proteomics analysis of which is in the form of 2D electrophoresis gel so as to separate, identify and measure the molecular weight. Another technology is highly sensitive mass spectrometry, and High-Performance Liquid Chromatography (HPLC).Keywords: proteomics, database, proteomics technology
Produksi Asam Laktat oleh Lactobacillus delbrueckii subsp. bulgaricus dengan Sumber Karbon Tetes Tebu Nurjannah, Laita; Suryani, Suryani; Achmadi, Suminar Setiati; Azhari, Azmi
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 9, No 1 (2017): Vol.(9) No.1, April 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (964.784 KB) | DOI: 10.17969/jtipi.v9i1.5903

Abstract

Senyawa asam laktat sangat dibutuhkan di dunia industri. Namun produksi dengan menggunakan mikrob masih menggunakan bahan pangan sebagai substratnya. Alternatif substrat untuk produksi asam laktat  sebagai pengganti penggunaan bahan pangan  sangat diperlukan industri. Tetes tebu merupakan salah satu substrat yang kaya akan sumber  karbon yang dapat digunakan sebagai komponen media pertumbuhan bakteri. Ketersediaannya melimpah dan harganya murah. Tujuan penelitian ini adalah tetes tebu dapat digunakan sebagai alternatif  sumber karbon bakteri Lactobacillus delbrueckii subsp. bulgaricus untuk menghasilkan asam laktat. Langkah penelitian ini meliputi hidrolisis dan detoksifikasi tetes tebu, uji kualitatif gula pereduksi tetes tebu, analisis gula total dengan metode fenol sulfat, penentuan kurva pertumbuhan bakteri, produksi dan ekstraksi asam laktat, serta analisis kualitatif asam laktat dengan menggunakan kromatografi cair kinerja tinggi. Hasil penelitian menunjukkan bahwa tetes tebu dapat digunakan sebagai alternatif sumber karbon. Hal ini terbukti bakteri dapat tumbuh dengan baik ketika media diberi 0.5% tetes tebu. Konsentrasi gula total tetes tebu adalah 1090 g/L. Uji gula pereduksi menunjukkan hasil yang positif untuk uji Selliwanof, uji Benedict, dan uji Barfoed. Pertumbuhan optimum L. delbrueckii subsp. bulgaricus terjadi pada suhu 42°C dengan agitasi 150 rpm. Produksi asam laktat dilakukan selama 24 jam. Kadar asam laktat yang dihasilkan sebesar 2.80% dengan biomassa sel kering sebesar 0.002 g/L dan pH media fermentasi sebesar 4.0. Hasil analisis kualitatif kromatografi cair kinerja tinggi juga menunjukkan bahwa produk dari hasil fermentasi adalah asam laktat.Abstract. Lactic acid is needed as an industrial feed. However, by using a microbial production still uses food material as a substrate. Alternative substrates for the production of lactic acid is needed in industry. Molasses are potential substrates due to the richness in carbon. Molasses also widely available and low-cost material. The objective of the research is molasses can be used as a carbon source needed by Lactobacillus delbrueckii subsp. bulgaricus to produce lactic acid. This study consisted of  hydrolysis and detoxification of molasses, analysis qualitative test of reducing sugar from molasses, analysis of total sugar by phenol sulfuric acid, determination of bacterial growth, production and extraction of lactic acid, and analysis of lactic acid using high performance liquid chromatography. The results showed that molasses can be used as an alternative carbon source as indicated by growth of  bacteria when the media were given 0.5% molasses. Concentration of total sugar molasses was 1090 g/L. The reducing sugar test showed positive results for the Selliwanoff, Benedict, and Barfoed tests. The optimum of L. delbrueckii subsp. bulgaricus growth was at temperature of 42° C and 150 rpm of agitation. Production of lactic acid was conducted in 24 hours. The result of lactic acid from the production was 2.80%. The dry cell biomass was 0.002 g/ L at pH of  fermentation media was 4.0. Analysis HPLC also showed that lactic acid was the product of fermentation.
Front & Back Matter Volume 7 (2): December 2018 Azhari, Azmi
Scientiae Educatia: Jurnal Pendidikan Sains Vol 7, No 2 (2018): December (2018)
Publisher : Tadris IPA Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.42 KB) | DOI: 10.24235/sc.educatia.v7i2.4037

Abstract