Mohamad Rusdi Hidayat, Mohamad Rusdi
Balai Riset dan Standardisasi Industri Pontianak, Jl. Budi Utomo No.41 Pontianak 78243

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Biogas Production from Tofu Industrial Wastewater with Effective Microorganisms 4 (EM-4) as Biocatalyst Hidayat, Mohamad Rusdi
Biopropal Industri Vol 3, No 1 (2012)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

The addition of Effective Microorganims 4 (EM-4) activator against the biogas  production was done to utilize the tofu industrial wastewater as one of the substrate for biogas production so it can increase its economic value. The aim of this research was to determine the optimum EM-4 concentration as a biogas activator using tofu industrial wastewater as a substrate. This research was conducted by adding the EM-4 activator with different concentrations of 0.25%, 0.50%, 0.75%, and 1% into the 20 litre of tofu wastewater substrate. The process of biogas formation was observed through a pressure reading on pressure gauge tools on digester. The burning of biogas is done after the presure became constant. The optimal EM-4 activator addition for 20 litre of tofu wastewater substrate was 0.75%, with burning time is 14 minutes 56 seconds.
Search Region of Origin Honey Bee A. mellifera in Indonesia Region Using Mitochondrial DNA intergenic cox1/cox2 Hidayat, Mohamad Rusdi
Biopropal Industri Vol 2, No 2 (2011)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Apis mellifera is a favourite honey bee for the beekeepers throughout many countries. This species comprise of 24 subspecies. Based on phylogeography and morphometric evidences, these subspecies have been grouped into four lineage; namely the African (A), Western and Northern Europe (M), Southeastern Europe (C), and Near Eastern (O). Apis mellifera have been imported to Indonesia since 1972, and mostly from Australia. However, until recently there are no data about the A. mellifera subspecies and the origin. Therefore the objective of this research is to determine the lineage of A. mellifera in Indonesia based on mtDNA intergenic region between cox1/cox2 genes. In this region there are two DNA fragments, P and Q fragnant, that can be used to determine the A. mellifera lineage. The methodology used consist of samples collection, DNA isolation, DNA amplification, DNA restriction using DraI enzyme, DNA sequencing, and DNA alignment using Clustal X and MEGA spftwares. DNA fragment amplified by using E2 and H1 primer revealed a 863 bp. Digestion of the region with the DraI restriction enzyme revealed one haplotype, which consist of five DNA fragments. Based on DNA sequences and DNA alignment, A. mellifera in Indonesia was homologue with the C lineage. Its subspecies is A. m. ligustica that lived natively in Italy, they were imported to Indonesia from Australia
Pretreatment Technologies of Lignocellulosic Materials in Bioethanol Production Process Hidayat, Mohamad Rusdi
Biopropal Industri Vol 4, No 1 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Bioethanol is one type of biofuel that developed significantly. The utilization of bioethanol is not only limited for fuel, but also could be used as material for various industries such as pharmaceuticals, cosmetics, and food. With wide utilization and relatively simple production technology has made bioethanol as the most favored biofuel currently. The use of lignocellulosic biomass, microalgae, seaweeds, even GMO (Genetically modified organisms) as substrates for bioethanol production has been widely tested. Differences in the materials eventually led to change in the production technology used. Pretreatment technology in the bioethanol production using lignocellulosic currently experiencing rapid development. It is a key process and crucial for the whole next steps. Based on the advantages and disadvantages from all methods, steam explotion and liquid hot water methods are the most promising  pretreatment technology available.
KARAKTERISASI BAKTERI SELULOLITIK SIMBION RAYAP ASAL TAMAN NASIONAL BUKIT BAKA BUKIT RAYA, KALIMANTAN BARAT (Characterization of Cellulolytic Bacteria Termite Symbiont from Bukit Baka Bukit Raya National Park, West Kalimantan) Hidayat, Mohamad Rusdi; Hidayati, Hidayati
Jurnal Hasil Penelitian Industri Vol 27, No 2 (2014)
Publisher : Baristand Industri Banda Aceh

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Abstract

Cellulase is the third largest industrial enzyme worldwide. This enzyme isproduced by microorganisms. Termite is one of the arthropods that could utilize symbioticmicroorganisms in their gut to digest lignocellulose. The purpose of this study was tocharacterize the cellulolytic bacteria that have been previously isolated from termites gutoriginated from the Bukit Baka Bukit Raya National Park. Isolates used were isolateMRH.13.S, isolate MRH.13.AF, and isolate MRH.13.O2. Characterizations conductedwere on the determination of the isolates growth curve, as well as the determination of theoptimum pH and temperature. The results showed that isolate MRH.13.S have an optimumtime at 36 hours. While isolate MRH.13.AF isolates had optimum time at 24 hours. Bothisolates have optimum pH in acidic conditions, and optimum temperature of 60˚C. Andisolates MRH.13.O2 have an optimum time of 48 hours, with an optimum pH in acidic toneutral and optimum temperature of 60˚C.