R. Haryo Bimo Setiarto, R. Haryo Bimo
Pusat Penelitian Biologi LIPI Jalan Raya Jakarta-Bogor Km 46, Cibinong, Bogor

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SELEKSI BAKTERI ASAM LAKTAT PENGHASIL AMILASE DAN PULULANASE DAN APLIKASINYA PADA FERMENTASI TALAS

Jurnal Teknologi dan Industri Pangan Vol 26, No 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objective of this study were to select amylase and pululanase producing lactic acid bacteria (LAB) for taro fermentation and to find out the length of fermentation time that will produce short chain polysaccharide. Fourty one LAB isolates were selected based on the amylase and pululanase activity (U/mL). Three isolates of LAB i.e. Lactobacillus plantarum D-240, SU-LS67 and SU-LS59 demonstrated the highest enzyme activities among other strains. The amylase activity for those three isolates was 2.57, 2.70, and 2.50 U/mL, respectively and the pullulanase activity was 2.72, 2.88 and 2.91 U/mL, respectively. Genotypic identification was conducted for strains SU-LS59 and SU-LS67. Strains identification by sequencing the gene encoding 16S rDNA and phylogenetic analysis using Neighbor Joining method showed that both isolates were identical to Leuconostoc mesenteroides NBRC 100496T (AB681194 ) with a bootstrap value of 100%. Either single or mixed culture of L. plantarum D-240 and L. mesenteroides SU-LS 67 were then used as starter in taro fermentation and DP values of the taro starch were examined at various fermentation times (0, 6, 12, 18, 24 h). The results showed that applying 2% mixed culture (108 CFU/mL) of L. plantarum D-240 and L. mesenteroides SU-LS 67) at the ratio of 1:1 as starter in taro fermentation was found more effective than the single cultures due to its ability to hydrolize and generate starch with DP value around 27 after 18 h fermentation. Starch with DP values between 19-29 was considered suitable for the formation of resistant starch (RS) during autoclaving-cooling cycles. This finding might be advantageous as preliminary treatment for the production of RS-rich taro flour through autoclaving-cooling process.

Kinetika Enzim Nitrilase Dari Sel Utuh Rhodococcus spp Pada Biotransformasi Mandelonitril

Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Rhodococcus spp can be used as mandelonitrile substrate for carbon and nitrogen source in life cycle of metabolism. They have potential of activity biotransformation for produced (R/S)-mandelic acid from mandelonitrile. (R/S)-mandelic acid is an important biotransformation product for production of pharmaceuticals such as semisynthetic penicillins, cephalosporins, antitumor agents, antiobesity agents and antiinflamation agents. This research was conducted to determine the enzyme kinetics (Km and Vmax) of nitrilase from Rhodococcus TPIK, LP3, GLB5 (induction acetonitrile 1000 mM - mandelonitrile 100 mM) with florometric methods, spectrophotometer analysis (λ = 413 nm). This research was carried out by assaying nitrilase enzyme activities in various concentration of mandelonitrile subtrates which were between 10 mM – 100 mM with 10 mM interval. The result showed that the enzyme kinetics of nitrilase from Rhodococcus TPIK (Km was 72.303 mM and Vmax was 2.075 mM/ml cell/minute), Rhodococcus LP3 (Km was 47.048 mM and Vmax was 1.942 mM/ml cell/minute), Rhodococcus GLB5 (Km was 34.375 mM and Vmax was 2.083 mM/ml cell/minute). Nitrilase enzyme from Rhodococcus GLB5 have smallest Km value. So we can interpretationed, this enzyme have good complexity Enzyme-Substrate, high affinity with substrate, and high speed reaction for forms product mandelic acid.

PROSPEK DAN POTENSI PEMANFAATAN LIGNOSELULOSA JERAMI PADI MENJADI KOMPOS, SILASE DAN BIOGAS MELALUI FERMENTASI MIKROBA

JURNAL SELULOSA Vol 3, No 02 (2013): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Microbial fermentation can processes lignocelluloses rice straw become several commodity with high value economics. Aerobic fermentation will results compost, and carbondioxyde. Meanwhile, anaerobic fermentation will results silase, biogas, and sludge. This review focused to doing comparative study about prospect and potency bioconversion rice straw to become biogas, silase, and compost by using microbial fermentation based on technoeconomical parameter. Compost from rice straw fermentation using Trichoderma sp., Trametes sp., and Aspergillus sp. which have quality: C/N ratio (18.88), C (35.11%) , N (1.86%), P2O5 (0.21%), K2O (5,35%), water activity (55%), Ca (4.2%), Mg (0.5%), Cu (20 ppm), Mn (684 ppm) and Zn (144 ppm). Moreover, silase from rice straw fermentation using Lactobacillus bulgaricus, Lactobacillus plantarum, and Plediococcus pentosomonas which have quality like acid flavour, it can not growth with fungi, it have green and yellow colour, pH 4.2, lactic acid composition (1.5-2.5%), butyric acid concentration (0.1%), acetic acid composition (0.5-0.8%), and composition N-NH3 (5-8%). Furthermore, biogas from rice straw fermentation using metanogenic bacteria (Methanobacterium and Methanobacillus) which have value 590 – 700 kcal per cubic, so it can results electricity energy from 1.25 to 1.50 kWH. It was equivalen with 0.5 kg liquid natural gas, 0.5 litres premium, and 0.5 litres diesel oil. From technoeconomical aspect, it can concluded that prospect bioconversion rice straw become biogas is more profitable to be applied in Indonesia based on parameter Benefit Cost Ratio because it will be gotten two advantages from once processes which are product biogas and compost from residual sludge.Keywords: rice straw, fermentation, compost, silase, biogas ABSTRAK Fermentasi mikroba mampu mengolah limbah lignoselulosa jerami padi menjadi beberapa komoditas dengan nilai ekonomi yang tinggi. Fermentasi secara aerob akan menghasilkan kompos, dan karbondioksida. Sementara itu proses fermentasi secara anaerob akan menghasilkan silase, biogas dan sludge. Review ini bertujuan melakukan studi komparatif terhadap prospek dan potensi pemanfaatan jerami padi menjadi biogas, silase maupun kompos melalui fermentasi mikroba ditinjau dari aspek teknis maupun ekonomis. Kompos jerami padi hasil fermentasi Trichoderma sp., Tremates sp., dan Aspergillus sp. memiliki kualitas: rasio C/N (18,88), C (35,11%) , N (1,86%), P2O5 (0,21%), K2O (5,35%), kadar air (55%), Ca 4,2%, Mg (0,5%), Cu (20 ppm), Mn (684 ppm) dan Zn (144 ppm). Kualitas produk silase jerami padi hasil fermentasi Lactobacillus bulgaricus, Lactobacillus plantarum, dan Plediococcus pentosomonas adalah berbau asam, tidak berjamur, berwarna hijau kekuningan, memiliki pH 4,2; kandungan asam laktat 1,5-2,5%, kandungan asam butirat 0,1%, kandungan asam asetat 0,5-0,8%; dan kandungan N-NH3 5-8%. Biogas jerami padi hasil fermentasi bakteri metanogenik Methanobacterium dan Methanobacillus memiliki nilai kalor 590 – 700 kkal per kubik, sehingga mampu membangkitkan energi listrik sebesar 1,25 – 1,50 kWH dan dapat disetarakan dengan 0,5 kg gas alam cair, 0,5 liter bensin, dan 0,5 liter minyak diesel. Secara ekonomi, prospek pemanfaatan jerami padi menjadi biogas lebih menguntungkan untuk diaplikasikan di Indonesia berdasarkan parameter Benefit Cost Ratio karena akan diperoleh dua keuntungan dalam satu kali proses produksi yaitu produk biogas serta kompos yang berasal dari sludge residu.Kata kunci: jerami padi, fermentasi, kompos, silase, biogas