Adriana Hiariej, Adriana
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PROFIL LINGKUNGAN TUMBUH PISANG TONGKAT LANGIT (Musa troglodytarum L.)DI KABUPATEN MALUKU TENGAH Hiariej, Adriana
BIOWALLACEA Vol 1, No 1 (2015): Bio Wallacea Vol 1 No 1
Publisher : Program Studi Biologi FMIPA Universitas Mataram

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Abstract

Di semua propinsi di Indonesia tumbuh baik pisang liar maupun pisang yang sudah di budidayakan.Pisang Tongkat Langit dengan nama ilmiah Musa troglodytarum L. adalah jenis pisang yang tandannya tegak ke atas dan merupakan tanaman endemik di daearah Indonesia Timur, yaitu Maluku dan Papua. Oleh masyarakat Maluku dikenal dengan nama ”Pisang Tongka Langit”. Propinsi Maluku merupakan bagian dari wilayah Timur Indonesia, dijuluki daerah seribu pulau terdiri dari pulau-pulau kecil yang dipisahkan oleh laut.Wilayahnya bervariasi baik dari faktor geografis, edafik dan iklim.Perbedaan tersebut tentu sangat berpengaruh pada keragaman fenotipik dan genetik organisme termasuk pisang Tongkat Langit sebagai akibat interaksi dengan lingkungan.Penelitian ini bertujuan untuk mengetahui profil lingkungan tumbuh pisang Tongkat Langit di kabupaten Maluku Tengah.Metode eksplorasi dilakukan untuk mendeskripsi profil lingkungan meliputi suhu, kelembaban, salinitas sedangkan sampel tanah dari lingkungan tumbuh diambil untuk dianalisis, di laboratorium kimia tanah UB Malang dengan metode kering oven. Data profil lingkungan dideskripisikan secara deskriptif dan dianalisis dengan analisis cluster Indeks Bray Curtis. Hasil penelitian menunjukkan bahwa terdapat keragaman profil lingkungan tumbuh meliputi suhu (13-32°C), kelembaban (8-30%), salinitas( 0.21- 1.5 mS), pH 5.2-7.3), C.organik (1.83-5.23%), N. total (0.21-0.59%), C/N (8-12), P (2.22-23.19 mg kg-1), K, Na, Ca, Mg, KTK, jumlah basa (0.16-1.41, 0.24-1.82, 5.02-23.81, 1.07-3.32, 2.22-23.19, 8.64-27.68 me/100g), KB (43-92%), pasir (3-70%), debu (25-78%) dan liat (0-28%). Hasil analisis cluster menunjukkan bahwa terdapat 2 cluster utama dengan nilai similaritas 0,68-0,96. Dari keragaman profil lingkungan dapat dijelaskan ketersedian unsur hara sangat dipengaruhi oleh reaksi pH tanah, bahan organik, KTK, KB tekstur dan struktur tanah serta kehidupan mikroorganisme yang penting bagi pertumbuhan maupun produksi pisang tongkat langit.Untuk suhu, kelembaban, dan salinitas.dikatakan masih mendukung pertumbuhan pisang tongkat langit di kabupaten Maluku Tengah.  Kata Kunci :Musa troglodytarum L., Lingkungan tumbuh, Maluku Tengah
GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Salamena, Fuadiska; Hiariej, Adriana; Seumahu, Cecilia Anna
Agrotech Journal Vol 3, No 1 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i1.524

Abstract

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species