M. Lutfi Firdaus, M. Lutfi
University of Bengkulu

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BIOSINTESIS NANOPARTIKEL PERAK MENGGUNAKAN EKSTRAK BUAH Passiflora flavicarva (MARKISA) UNTUK MENDETEKSI LOGAM BERAT Maryani, Dina; Firdaus, M. Lutfi; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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The aim of this research was to synthesize silver nanoparticle (NPP) by using Passiflora flavicarva (Markisa) fruit extract as detector of heavy metal. The NPP particles produced were determined by their optimum condition on the ratio of the volume of  Passiflora flavicarva passion fruit extract to AgNO3 and the reaction time. The silver nanoparticles produced under optimum conditions will be tested for selectivity against several metals that are often present in the environment, once the selective metal is known then it can be sensitized to the metal using a UV-Vis spectrophotometer to determine the minimal concentrations that can be detected by the silver nanoparticles Formed. The results showed that Passiflora flavicarva passion fruit extracts could be used for the biosynthesis of  NPP particles because they contain vitamin C and flavonoids capable of reducing Ag+ to Ag0 in the form of NPP. The optimal conditions for forming NPP particles are in the 1: 2 ratio with a 2 hour reaction time. From the results of selectivity test of NPP to some heavy metals that often exist in environment, it is known that NPP selective to mercury metal. It was found that selective mercury was followed by a sensitivity test with a UV-Vis spectrophotometer, with sensitive NPP  up to a concentration of 6.7 ppm. From the results of this study it can be concluded that NPP particles formed can be used to detect mercury metal up to a minimum concentration of 6.7 ppm.
PEMANFAATAN ARANG AKTIF SABUT KELAPA SAWIT SEBAGAI ADSOBEN ZAT WARNA SINTETIS REACTIVE RED-120 DAN DIRECT GREEN -26 Puspita, Melfi; Firdaus, M. Lutfi; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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The problem of environmental polution caused by waste of batik industry lately is increasing, so it needed a method to overcome that problem. The aim of this study was to determine the ability of activated charcoal from coconut fiber palm in adsorbing Reactive Red-120 and Direct Green-26 dyes in waste of batik along with determining the parameters of isotherms adsorption using UV-Vis Spectrophotometer analysis method. Variations of pH, contact time, adsorbent weight and temperature were carried out as variable to obtain optimum conditions of the adsorption process. The optimum of conditions for Reactive Red-120  occured at pH 3 and a contact time of 30 minutes, while Direct Green-26  occurred at pH 4 and a contact time of 40 minutes, with each adsorbent weight 150 mg and the temperature 30 °C. Adsorption isotherms determined by Freundlich and Langmuir models with maximum adsorption capacity (Qmax) were obtained for the Reactive Red-120  was 400 mg/g, while Direct Green-26 is 169 mg/g. 
PENENTUAN KESETIMBANGAN, TERMODINAMIKA DAN KINETIKA ADSORPSI ARANG AKTIF TEMPURUNG KELAPA SAWIT PADA ZAT WARNA REACTIVE RED DAN DIRECT BLUE Sari, Rensy Aula; Firdaus, M. Lutfi; Elvia, Rina
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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This perpose of this research was to know the isotherm, kinetics and thermodynamics adsorption of synthetic  dye onto activated carcoal from palm oil shell. The synthetic dye were Reactive Red and Direct Blue. The model of isotherm were Langmuir and Freundlich, kinetics adsorption tested were Pseudo orde-1 and Pseudo orde-2, and also thermodynamics were free energy change (?Go), enthalpy change (?Ho), and entropy change (?So). The dominant isotherm adsorption was isotherm Freundlich, kinetic adsorption Pseudo 2 th order  with the value (?So), (?Go) and (?Ho), Reactive Red were 0,0028 Kj/mol.K , -3,93 Kj/mol, and -36,12 Kj/mol respectively. Whereas in direct blue were 0,0034 Kj/mol.K, -1,67 Kj/mol,and -30,16 Kj/mol respectively. 
PEMBUATAN NANOPARTIKEL PERAK (NPP) DENGAN BIOREDUKTOR EKSTRAK BUAH Muntingia calabura L UNTUK ANALISIS LOGAM MERKURI Sari, Purwo Ismaya; Firdaus, M. Lutfi; Elvia, Rina
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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The goal of this research was to make silver nanoparticles (NPP) with a cherry fruit extract bioreductor which was used for mercury metal analysis by using a digital image method. The process of making silver nanoparticles had done by a bottom up method involving a reduction reaction. The precursors used were 1 mM AgNO3 solution and as a bioreductor was an antioxidant compound which were in cherry fruit extract. Variation of the volume ratio between cherry fruit extract and 1 mM AgNO3 solution was 1: 3, 1: 4, 1: 5, 1: 6, and 1: 7 and the variation of contact time were 5, 15, 30, 45, 1, 2, 3 hours, and 1, 2, 7 days. The most optimum results of silver nanoparticles were synthesized with 1 : 4 volume ratio and 1 hour contact time. From the results of selectivity test, it was known that silver nanoparticles were made selective toward Hg and Fe metal. The sensitivity test toward Hg metal, it was known that NPP can detect mercury metal with the smallest concentration of 16,7  ppb.
A Rapid and Sensitive Diagnosis of Typhoid Fever Based On Nested PCR-Voltammetric DNA Biosensor Using Flagellin Gene Fragment Hartati, Yeni Wahyuni; Wyantuti, Santhy; Firdaus, M. Lutfi; Auliany, Nurul; Surbakti, Rini; Gaffar, Shabarni
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

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Typhoid fever caused by Salmonella typhi is an important issue for public health in the world. Laboratory methods for rapid and sensitive diagnosis are very important for disease management. The purpose of this study was to determine the performance of nested PCR–voltammetric DNA biosensor using flagellin gene (fla) of S. typhi as a marker. The differential pulse voltammetry using pencil graphite electrode was applied to measure the guanine oxidation signal of probes vs synthetic target stDNA and probes vs fla PCR product hybridizations. The probe DNA selectivity was examined by hybridized probes vs non-complementary sequence. The result showed that the first round nested PCR product can not be visualized by agarose electrophoresis, whereas using the voltammetric biosensor methods can be detected both for the first or second round nested PCR product. The average peak current of hybridized probe vs first and second round of PCR product was 2.32 and 1.47 μA respectively, at 0.9 V. Detection of the DNA sequences of the infectious diseases from PCR amplified real sample was also carried out using this voltammetric DNA biosensor methods.
Inovasi Sistem Stack Microbial Fuel Cell menggunakan Substrat Limbah Rumen Sapi serta Implementasinya sebagai Media Pembelajaran Novarina, Diya; Swistoro, Eko; Firdaus, M. Lutfi; Medriati, Rosane
PendIPA Journal of Science Education Vol 2, No 3 (2018): October
Publisher : FKIP University of Bengkulu

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ABSTRACT[Innovation of microbial fuel cell stack system using cow rumen waste substrate and its implementation as a learning media]. The aims of this study are to: 1) describe the difference of Electric Motion (GGL), 2) describe the difference of electric power per unit area of the anode (Pa) which is produced between the series design MFC type series, parallel, mixed type 1 and mixed type 2, and 3) describe the significant difference of learning outcomes between the PBL learning model using MFC media with PBL learning model by using the electrical kit medium on dynamic electrical concept in SMA Muhammadiyah 4 Bengkulu. The free variable of this research are MFC stack system design (series, parallel, mixed types 1 and 2) and the dependent variable is GGL and electric power per unit of anode surface area. The results are obtained difference GGL and Pa between series design, parallel, mixed types 1 and 2 by comparison are 3: 1: 1,5: 2 whereas Pa ratio is 1: 10: 6: 2 with maximum GGL is obtained in series design of 3,29 V and Pa maximum in parallel circuit 21,76 mW / m2. Implementation of MFC series as a learning media used Problem Based Learning model on physics learning Implementation of MFC circuit with research design using one group pretest-posttest design. The testing of hypothesis with t-test shows tcount 2.739> ttable 2,001 and 95% significant level so it can be concluded there are significant differences in learning outcomes of PBL learning model by using MFC media with PBL learning model by using media Electric Kit on dynamic electrical concept in SMA Muhammadiyah 4 Bengkulu. Keywords: Stack Microbial Fuel Cell (MFC) Sistem; Rumen Cow Liquid Waste; Learning Media.
Pengukuran kualitas dan muka air tanah di sekitar PT. Bio Nusantara Teknologi untuk mendukung proses pembelajaran fisika Septianingsih, Dwi Alfina; Firdaus, M. Lutfi; Farid, Muhammad
PendIPA Journal of Science Education Vol 2, No 1 (2018): February
Publisher : University of Bengkulu

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ABSTRAKPenelitian ini bertujuan untuk mengetahui potensi air tanah di sekitar perkebunan sawit di Desa Pondok Kelapa-Bengkulu yang dilakukan dengan menggunakan resistivity-meter metode geolistrik, konfigurasi elektroda Wenner. Data pengukuran muka air tanah dan hasil kualitas air diperoleh pada 4 lokasi penelitian. Interpretasi kurva resistivitas dengan Res2dinv menunjukkan bahwa daerah Pondok Kelapa masih memiliki potensi air tanah dengan jarak 0,5 m. Keempat lokasi diantaranya: (1) pada rata-rata kedalaman 1,967 m dengan pH 6, besi 0,0021 mg/L dan kekeruhan 54,16 NTU; (2) pada rata-rata kedalaman 2,935 m dengan pH 6, besi 0,0022 mg/L dan kekeruhan 37,12 NTU; (3) pada rata-rata kedalaman 2,240 m dengan pH 5,8, besi 0,0021 mg/L dan kekeruhan 35,65 NTU; dan (4)pada rata-rata kedalaman 2,678 m dengan pH 5,7, kandungan besi 0,0011 mg/L dan kekeruhan 19,02 NTU. Hasil penelitian menyimpulkan bahwa terdapat kecenderungan hubungan terbalik antara kualitas air dan muka air tanah dari segi kekeruhan. Hasil penelitian ditampilkan sebagai penunjang proses pembelajaran Fisika yakni pengembangan LKPD berbasis service learning materi Hukum Ohm penerapan pengukuran geolistrik yang berorientasi pada pengembangan reflective thinking dengan desain ADDIE (Analisys, Design, Development, Implementation, and Evaluation). Kemampuan hasil belajar 21 siswa SMK kelas X-ATP (Agribisnis Tanaman Perkebunan) didapat dengan peningkatan hasil Ngain antara nilai pretest dan posttest sebesar 0,66 yakni berada dalam kategori sedang.Kata Kunci: Kualitas air; muka air tanah; PT Bio Nusantara Teknologi; pembelajaran fisika.
Implementasi Model Discovery Learning Pada Materi Aplikasi Gelombang Elektromagnetik Analisis Pengotor Berbagai Minyak Goreng Sawit dengan Spektrofotometri UV-Vis Wulandari, Tria; Nirwana, Nirwana; Firdaus, M. Lutfi
PendIPA Journal of Science Education Vol 3, No 2 (2019): JUNE
Publisher : University of Bengkulu

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ABSTRACT[Implementation of Discovery Learning Model on Electromagnetic Wave Application Material of Various Palm Oil Impurity Analysis With Spectrophotometry UV-Vis].The aims of this study was to describe the differences between the learning outcomes of students who learn to use the learning model of Discovery Learning with students studying physics learning conventionally onwaves electromagnetic material.  The method used quasi experimental research with Pretest-Posttest Control GroupDesign research conducted at SMA Negeri 8 Lubuklinggau. Sample research used 2 classes i.e. class X.3 as a experimental class and class X.2 as a control class. Based on the results of data analysis it can be concluded that there was a difference between learning outcomes of students who learn to use the learning model of Discovery Learningwith students studying in conventional method. The data views from the average final value obtained after being given the treatment. The average value of experimental classes after being given the treatment model of learning by Discovery Learning was 76.27, while the average value of control class after being given treatment to conventional learning was 67.00.Keywords: Discovery; Learning;Electromagnetic Waves; Spectrophotometry UV-Vis.(Received August 23, 2018; Accepted March 2, 2019; Published June 18, 2019)ABSTRAKPenelitian ini bertujuan untuk menjelaskan perbedaan hasil belajar antara siswa yang belajar menggunakan model pembelajaran Discovery Learning (DL) dengan siswa yang belajar secara konvensional pada pembelajaran fisika dengan materi gelombang elektromagnetik.  Metode penelitian yang digunakan adalah penelitian eksperimen semu (Quasi Eksperimen) dengan desain penelitian Pretest-Posttest Control GroupDesign, yang dilakukan di SMA Negeri 8 Lubuklinggau. Sampel penelitian yang digunakan berjumlah 2 kelas yakni kelas X.3 sebagai kelas eksperimen dan kelas X.2 sebagai kelas kontrol. Berdasarkan hasil analisis data dapat disimpulkan bahwa terdapat perbedaan hasil belajar antara siswa yang belajar menggunakan model pembelajaran Discovery Learning (DL) dengan siswa yang belajar secara konvensional. Data tersebut dilihat dari nilai rata-rata akhir yang diperoleh setelah diberi perlakuan. Nilai rata-rata kelas eksperimen setelah diberi perlakuan dengan model pembelajaran Discovery Learning sebesar 76,27, sedangkan nilai rata-rata kelas kontrol setelah diberi perlakuan pembelajaran konvensional sebesar 67,00.Kata Kunci:Discovery; Learning; GelombangElektromagnetik; Spektrofotometri UV-Vis.
ANALISIS ION MERKURI (II) MENGGUNAKAN NANO PARTIKEL PERAK TERIMOBILISASI PADA KERTAS SARING Meileza, Nessi; Firdaus, M. Lutfi; Elvinawati, Elvinawati
Alotrop Vol 2, No 2 (2018)
Publisher : FKIP University of Bengkulu

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The aims of this research is improve stability and sensitivity of silver nanoparticles (NPP) immobilizatied on filter paper. Silver nanoparticles synthesized by reducing the ascorbic acid using a chemical reduction method. Immobilization NPP on filter paper is done with the technique of immobilization (immunosystem) is the process of molecular bonding reagent in supporting materials, so physically bound NPP the surface of filter paper is composed of cellulose fibers. The results obtained for the stability of immobilization NPP on filter paper can be last up to stable 60-daywhile liquid NPP stability can only last for 30 days. To maintained the stability ofimmobilization on filter paper is saved in a closed box. For the determination of sensitivity of immobilization NPP in the filter paper test is performed against the mercury (II) ion with each different concentrations. The results obtained for sensitivity of immobilization NPP on the filter paper is analyzed in digital image can be detect mercury (II) ion until the concentration of ppb. The results of measurement Limit of Detection (LOD) or limit the smallest NPP can detect mercury (II) ion is under 0.863 ppb, while liquid NPP able to detect only concentration 1.448 ppb.. The results of this research NPP immobilization on filter paper can be an alternative to increasingstability and sensitivity of NPP compared with liquid  NPP, and can be applied into the colourimetry indicator is inexpensive and practical to analysis of mercury (II) ion in the digital image.