Ananto Ali Alhasyimi, Ananto Ali
Program Studi Master Ilmu Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Antioxidant potency of mangosteen peel extract topical application in reversing reduced orthodontic brackets tensile strength after bleaching

Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 4 (2017): (December 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Nowadays, cosmetic dentistry has become an ever-increasing requirement with interest in it growing over time. Bleaching is one of the popular cosmetic treatments that has been proven to diminish the tensile bond strength (TBS) of orthodontic brackets attached to bleached teeth. Mangosteen peel (MP) extract contains antioxidants that may potentially reverse the reduction in TBS. Purpose: The purpose of this study was to evaluate the effect of MP extract on the post- bleaching TBS of brackets. Methods: The reported research constitutes an experimental in vitro study conducted on a total of 120 maxillary first premolar teeth randomly divided into six groups (n = 20) as follows: negative-control (NC: no bleaching), positive-control (PC: bleaching + no treatment), and the treatment groups (bleaching + 10% sodium ascorbate (SA), 10% (MP10), 20% (MP20) and 40% (MP40) MP extract gel). Post-treatment, the brackets were bonded using Transbond XT and TBS testing was performed using a Universal Testing Machine. The ARI was examined by means of a stereoscopic microscope, while enamel morphological changes were observed through a Scanning Electron Microscope. The TBS-generated data was analyzed by means of Anova and Tukey tests. For the Adhesive Remnant Index, a Kruskal-Wallis analysis test was performed. Results: There was a significant TBS difference (P = 0.001) between the various groups. The PC group showed the significantly highest TBS compared to the others (8.33 ± 3.92 MPa), whereas NC demonstrated the lowest (4.15 ± 2.27 Mpa). The TBS value of the MP40 group was considerably higher than other groups treated with antioxidants (7.87 ± 3.26 MPa). The failure of orthodontic brackets using MP extract mostly occurred at the adhesive-bracket interfaces. Conclusion: Topical application of 40% mangosteen peel (MP) extract as an antioxidant after bleaching was effective in reversing the reduced post-bleaching tensile bond strength (TBS) of orthodontic brackets.

Comparison of esthetic smile perceptions among male and female Indonesian dental students relating to the buccal corridors of a smile

Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 3 (2017): (September 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: A smile constitutes a form of measurement as to whether or not an orthodontic treatment has proved successful. A smile is said to be ideal if a balance exists between the shape of the face and teeth. One benchmark used to assess the quality of an ideal smile is that of buccal corridors. These are formed of the black space between the lateral edge of maxillary posterior teeth and the corner of the lip which appears during the action of smiling. Evaluating the contrasting perceptions of male and female smiles based on buccal corridor aspects is considered important to identifying the specific qualities an ideal smile. Purpose: The purpose of this study was to determine the difference between the perceptions of an ideal smile held by Indonesian dental students of both genders based on buccal corridors. Methods: A total of 36 dental students, equally divided between male and female students and ranging in age from 18-21 years old, were enrolled in this study. The smiles of all subjects were photographed from the front for later assessment by the subjects themselves. Assessment was undertaken twice, with a two-week interval between the first and second, by comparing subjects’ photographs with reference pictures of buccal corridors. Data gathered were analyzed by using kappa-statistic and U-Mann Whitney. Results: The results indicated that all the subjects showed a good level of coincidence in their analysis (κ=0.76). Statistical analysis showed that the score of 0.123 (p>0.05) was shown in U-Mann Whitney. Conclusion: Indonesian male and female dental students have the same perception of an aesthetic smile with regard to its buccal corridor.

Rinsing effect of 60% bay leaf (Syzygium polyanthum wight) aqueous decoction in inhibiting the accumulation of dental plaque during fixed orthodontic treatment

Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 1 (2017): (March 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Fixed orthodontic appliance patients have a high risk to increasing plaque accumulation in tooth surface due to the presence of fixed appliance components. Using mouthwash with antibacterial agent from herbal material can control dental plaque accumulation. Bay leaf (BL) contains active compounds such as essential oil, tannin and flavonoid that have been known as an antibacterial agent. Purpose: The purpose of this study was to determine the effect of rinsing with 60% of BL aqueous decoction to the accumulation of dental plaque in fixed orthodontic appliance patients. Method: This research was an experimental clinical research with pretest and post-test control group design conducted on 20 subjects with age ranged between 18-25 years old. All subjects were undergoing the last stage (finishing) of fixed active orthodontic treatment. The subjects were instructed to rinse with 60% of BL aqueous decoction and 0.2% chlorhexidine as a control. Wash out period that needed between rinsing with 60% of BL aqueous decoction and rinsing with 0.2% chlorhexidine was 7 days. Each mouthwash used routinely for 7 days with same duration and intensity. Plaque scoring was measured before and after each treatment using Orthodontic Plaque Index (OPI). Result: One-way Anova test showed that there was a significant difference in the plaque index before and after treatment between the group of rinsing with 60% of BL aqueous decoction and group of rinsing with chlorhexidine (p<0.05). Conclusion: Rinsing with 60% BL aqueous decoction can decrease the accumulation of dental plaque in fixed orthodontic appliance patients.

Pengaruh Implantasi Subkutan Logam Kobalt Kromium sebagai Bahan Alternatif Mini Screw Orthodontics terhadap Reaksi Jaringan Kelinci Albino

Majalah Kedokteran Gigi Indonesia Vol 1, No 1 (2015): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

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Abstract

Mini screw orthodontics merupakan alat yang digunakan dalam perawatan ortodonsia untuk kasus yang membutuhkan absolute anchorage. Mini screw yang tersedia terbuat dari logam nikel titanium atau stainless steel yang pada beberapa pasien menyebabkan reaksi pada jaringan. Uji implantasi adalah uji yang dilakukan untuk menentukan biokompatibilitas medical device yang berkontak langsung dengan jaringan hidup. Respon jaringan terhadap jejas pasca implantasimempengaruhi derajat pembentukan jaringan granulasi, reaksi benda asing, dan fibrosis. Penelitian ini bertujuan untuk mengetahui efek lokal implantasi kobalt kromium sebagai alternatif bahan mini screw orthodontics terhadap jaringan subkutan kelinci albino. Subjek penelitian berupa 20 ekor kelinci jantan albino yang digunakan untuk 2 kelompok(perlakuan dan kontrol negatif). Kobalt kromium dibuat lempengan diameter 10 mm dan ketebalan 1 mm. Pada kelompok perlakuan, material diimplankan pada jaringan subkutan dorsum kelinci dengan insisi sampai terbentuk poket subkutan, dasar poket tidak lebih dari 10 mm dari garis awal insisi dan dilakukan suturing. Kelompok kontrol hanya dilakukanincisi kemudian disuturing. Secara makroskopis 24 jam pasca implantasi, daerah implan diamati terjadinya edema, hematoma, enkapsulasi dan tanda-tanda inflamasi. Evaluasi secara mikroskopis pada hari ke 14 pasca implantasi, diamati perubahan histopatologis infiltrasi sel-sel inflamasi, kemudian rerata hasil diuji dengan uji independent samplet-test untuk mengetahui apakah ada perbedaan jumlah sel-sel inflamasi pada kelompok perlakuan dan kontrol. Hasil pengamatan makroskopis, kelompok perlakuan maupun kontrol hanya terlihat tanda inflamasi ringan berupa eritema. Rerata dan Simpangan baku jumlah PMN, limfosit, sel plasma, makrofag, giant cell, neovaskularisasi, fibrosis kobalt kromium dan kontrol berturut turut yaitu (0,6 ± 0,49; 0,7 ± 0,48); (0,9 ± 0,87; 1,0 ± 0,67); (0,6 ± 0,24; 0,6 ± 0,21); (1,4 ± 0,84; 0,9 ± 0,74); (0,5 ± 0,27; 0); (0,6 ± 0,33; 0,7 ± 0,48); (0,5 ± 0,27; 0,1 ± 0,02). Hasil t-test menunjukkan perbedaan yang tidak signifikan (p>0,05) pada 6 parameter inflamasi sedangkan parameter giant cell menunjukkan perbedaan yang signifikan (p<0,05). Kesimpulan penelitian ini adalah implantasi subkutan logam kobalt kromium menyebabkan reaksi jaringan berupa infiltrasi giant cell selama 14 hari pasca implantasi sebagai respon tehadap benda asing. Effect Of Subcutaneous Implantation Of Cobalt Chromium As An Alternative Material For Mini Screw Orthodontics Against Albino Rabbit’s Tissue. Mini screw orthodontics is an instrument which is used in orthodontic treatment for some cases that require absolute anchorage. Available mini screws are made of nickel titanium or stainless steel alloy which in some patients may cause a reaction to the tissues. Implantation test is a test performed to determine the biocompatibility of medical devices that are directly contacted to the living tissue. Tissue response to injury after implantation affects the degree of formation of granulation tissue, foreign body reaction, and fibrosis. The aim of this study is to determine the local effect of implantation of cobalt chromium as an alternative material for mini screw orthodontic against the subcutaneous tissue of albino rabbits. The subjects of study were 20 male albino rabbits divided into two groups (treatment and negative control groups). Cobalt chromium was made in the form of discs with 10 mm in diameter and 1 mm in thickness. In the treatment group, the material was implanted in the subcutaneous tissue of the dorsum of the rabbits with an incision to form a subcutaneous pocket. The pocket base was not more than 10 mm from the initial line of incision then suturing was performed. The control group only had incision then sutured to maintain aseptic conditions. Macroscopically 24 hours after implantation, the implant area was observed related to the occurrence of oedema, haematoma, encapsulation and signs of inflammation. The evaluation on day 14 post-implantation shows the histopathologic changes observed by the infiltration of inflammatory cells types. The average of the results was analyzed using independent sample t-test. The results of the macroscopic observation shows that the treatment and control group were only seen of mild inflammatory signs included erythema. The mean and standard deviation of the amount of PMN, lymphocyte, plasma cells, macrophage, giant cell, neovascularisation, and fibrosis from cobalt chromium and control groups were (0.6 ± 0.49; 0.7 ± 0.48); (0.9 ± 0.87; 1.0 ± 0.67); (0.6 ± 0.24; 0.6 ± 0.21); (1.4 ± 0.84; 0.9 ± 0.74); (0.5 ± 0.27; 0); (0.6 ± 0.33; 0.7 ± 0.48); (0.5 ± 0.27; 0.1 ± 0.02). The results of t-test show a significant difference in giant cell (p<0.05), while the six others did not (p>0.05). The conclusion of this study is that the cobalt chromium alloy can affect the subcutaneous tissue by inducing giant cell infiltration on day 14 post-implantation as the result of foreign body reactions.

INDUKSI RE-EPITELISASI PADA PROSES PENYEMBUHAN LUKA GINGIVA OLEH APLIKASI TOPIKAL EKSTRAK DAUN SAGE (Salvia officinalis L.) KONSENTRASI 50% (Kajian In Vivo Pada Tikus Sprague Dawley)

B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 3, Nomor 1, Juni 2016
Publisher : Universitas Baiturrahmah

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Abstract

Luka pada gingiva sering terjadi baik disengaja maupun tidak disengaja. Luka adalah rusaknya kesatuan atau komponen jaringan. Penyembuhan luka merupakan reaksi jaringan yang rusak untuk mengembalikan fungsi jaringan tersebut. Salah satu proses yang terlibat dalam penyembuhan luka adalah re-epitelisasi. Sage (Salvia officinalis L.) merupakan tanaman yang berpotensi untuk penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun sage konsentrasi 50% secara topikal terhadap reepitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague dawley. Dua puluh empat ekor tikus Sprague dawley dibagi dalam 2 kelompok, perlakuan dan kontrol. Perlukaan pada gingiva labial tikus dibuat dengan menggunakan punch biopsy diameter 2,5 mm. Luka pada kelompok perlakuan diberi ekstrak daun sage konsentrasi 50% dan pada kelompok kontrol diaplikasikan iod gliserin 2 kali sehari selama 1 menit secara topikal. Tiga ekor tikus dari masing-masing kelompok dikorbankan pada 1, 3, 5 dan 7 hari setelah perlukaan. Jaringan luka diambil, diproses secara histologis dan dilakukan pengecatan dengan menggunakan metode Hematoksilin Eosin (HE). Pengukuran ketebalan jaringan epitel gingiva dilakukan menggunakan mikrometer okuler digital. Data ketebalan epitel dianalisis menggunakan paired samples ttest. Hasil pengukuran ketebalan jaringan epitel gingiva dengan paired samples t-test menunjukkan perbedaan yang signifikan antara kelompok perlakuan dan kelompok kontrol pada 3, 5 dan 7 hari setelah perlukaan. Kesimpulan dari penelitian ini adalah pemberian ekstrak daun sage konsentrasi 50% dapat menginduksi re-epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague dawley serta lebih efektif dibandingkan iod gliserin.