Kwartarini Murdiastuti, Kwartarini
Department of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Articles
5
Documents
The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue’s Sprague dawley Rats

The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : The Indonesian Journal of Dental Research

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Abstract

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd  (group C), 5th  (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.

Antimicrobial effect of chlorine dioxide on Actinobacillus actinomycetemcomitans in diabetes mellitus rats treated with insulin

Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 1 (2012): (March 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Periodontitis is a chronic inflammatory disease of periodontal tissues. Etiology of periodontal disease includes Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) which is the most predominant disease-causing bacteria found in the gingival sulcus. Periodontitis can be exacerbated by the systemic disease, such as diabetes mellitus considered as a metabolic disease characterized by hyperglycemia due to insulin deficiency. Treatment of periodontitis is then required in patients with type I diabetes to avoid radical reaction that can not only cause bleeding, but can also prevent infection, as a result, topical antimicrobial therapy and blood glucose control are required. Topical antimicrobial chlorine dioxide is a disinfectant that is effective in killing A. actinomycetemcomitans. Purpose: This study is aimed to determine the effects of topical antimicrobial chlorine dioxide gel or rinse on the number of A. actinomycetemcomitans in DM rats treated with insulin. Methods: 20 three month old male Wistar rats with weight of 170–200 grams were divided into four groups. First, periodontitis and DM were manipulated into all groups through aloksan injection with dose of 170 mg/kg. Those rats in group I were treated with insulin and chlorine dioxide gel, those in group II were treated with insulin and chlorine dioxide rinse, those in group III were treated with insulin only, and those in group IV were without treatment. In the third and seventh weeks, the number of A. actinomycetemcomitans was measured. The data was tested by using One-Way ANOVA test followed by LSD test. Results: The study showed that chlorine dioxide gel has a greater ability in reducing the number of A. actinomycetemcomitans than chlorine dioxide rinse although both are antimicrobials. Conclusion: It can be concluded that the use of chlorine dioxide gel can more effective to decrease the number of A. actinomycetemcomitans than chlorine dioxide rinse in DM rats treated with insulin therapy.Latar belakang: Periodontitis adalah suatu penyakit inflamasi kronis jaringan periodontal. Etiologi penyakit periodontal diantaranya adalah A. actinomycetemcomitans yang merupakan bakteri paling dominan penyebab penyakit yang terdapat pada actinomycetemcomitans yang merupakan bakteri paling dominan penyebab penyakit yang terdapat pada sulkus gingiva. Periodontitis yang terjadi diperparah adanya penyakit sistemik yaitu diabetes mellitus (DM) yang merupakan penyakit metabolik yang ditandai dengan hiperglikemi akibat defisiensi insulin. Perawatan periodontitis pada penderita DM tipe I adalah untuk Perawatan periodontitis pada penderita DM tipe I adalah untuk menghindari tindakan radikal yang dapat menyebabkan perdarahan dan mencegah terjadinya infeksi, sehingga digunakan terapi antimikroba topikal serta kontrol glukosa darah. Antimikroba topikal chlorine dioxide merupakan desinfektan yang efektif dalam membunuh A. actinomycetemcomitans. Tujuan: Penelitian ini bertujuan untuk mengetahui efek antimikroba topikal chlorine dioxide gel atau rinse terhadap jumlah A. actinomycetemcomitans pada tikus DM dengan insulin. Metode: Dua puluh ekor tikus Wistar, jantan, usia 3 bulan, berat 170–200 gram, dibagi menjadi empat kelompok. Semua kelompok sebelumnya dimanipulasi periodontitis serta DM dengan injeksi aloksan dosis 170 mg/kgBB. Kelompok I adalah tikus yang diterapi insulin dan chlorine dioxide gel, kelompok II diterapi insulin dan chlorine dioxide rinse, kelompok III diterapi insulin, serta kelompok IV adalah tikus tanpa dilakukan terapi apapun. Pada minggu ke-3 dan ke-7 dilakukan penghitungan jumlah A. actinomycetemcomitans. Hasil pengukuran di analisis menggunakan uji Anava 1 jalur dilanjutkan dengan uji LSD. Hasil: Penelitian menunjukkan bahwa chlorine dioxide gel mempunyai kemampuan lebih besar dalam menurunkan jumlah A. actinomycetemcomitans dibandingkan chlorine dioxide rinse, walaupun keduanya bersifat antimikroba. Kesimpulan: Dapat disimpulkan bahwa pemberian chlorine dioxide gel lebih efektif menurunkan jumlah A. actinomycetemcomitans dibandingkan kelompok yang diberi chlorine dioxide rinse pada tikus DM dengan terapi insulin.

Efek Radiasi Ionisasi terhadap Ekspresi mRNA Aquaporin-5 Kelenjar Submandibularis dan mRNA Aquaporin-3 Gingiva hal 15-20

Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

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Abstract

Latar Belakang. Aquaporin-5 (AQP5) di kelenjar submandibularis adalah salah satu anggota protein transmembran/ aquaporins (AQPs) yang memfasilitasi gerakan saliva sehingga mampu melewati membran sel. Di gingiva juga terekspresi aquaporin-3 (AQP3) yang diperkirakan juga berperan penting untuk memfasilitasi cairan sulkus gingiva sehingga dapat melewati jaringan ini. Sampai saat ini masih sedikit sekali informasi tentang dampak penggunaan radiasi ionisasi terhadap ekspresi aquaporins (AQPs), yang mendasari terjadinya xerostomia. Melalui pendekatan patologi molekuler, penelitian ini bertujuan untuk mengetahui efek radiasi ionisasi terhadap ekspresi mRNA AQP5 pada kelenjar submandibula dan mRNA AQP3 gingiva. Metodologi. Penelitian dilakukan pada 20 Rattus novergicus keturunan kedua, sehat, jantan, usia 3-4 bulan, berat badan ± 200 gr kemudian dibagi 2 kelompok, tanpa radiasi dan dengan radiasi C060dosis 10 gray pada ventral tikus. Pengambilan kelenjar submandibularis, dan gingiva dengan pembedahan. Seluruh sampel yang terkumpul dilakukan isolasi RNA dan dilanjutkan dengan pemeriksaan RT-PCR. Hasil. Penelitian ini menunjukkan adanya perbedaan gambaran ketebalan band/pita ketika dibatasi oleh primer untuk identifikasi ekspresi mRNA AQP-5 kelenjar submandibularis tikus serta ekspresi mRNA AQP3 gingiva tikus akibat radiasi ionisasi 10 Gy. Kesimpulan. Terdapat penurunan ekspresi mRNA AQP5 kelenjar submandibula dan mRNA AQP3 gingiva akibat radiasi ionisasi ekspresi mRNA AQP5 pada kelenjar submandibula dan mRNA AQP3 gingiva.

Differences in mucin expression in the submandibular glands of rats during peridontitis induction

Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 2 (2018): (June 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Porphyromonas gingivalis (Pg) produces lipopolysacharide (LPS) which acts as a stimulator of inflammation in periodontal tissues. Periodontitis-induced apoptosis and vacuolation of the salivary gland, therefore, causes hyposalivation. Mucin secretion is produced by the submandibular gland under stimulation by the cholinergic and adrenergic receptors. Both forms of stimulation influence the volume of mucin secretion. Mucin saliva plays an important role in the early stages of Pg colonization in the oral cavity. On the other hand, it serves to protect against bacterial invasion. Purpose: The aim of this research was to identify differences in mucin expression in the submandibular gland during periodontitis induction. Methods: 32 male Wistar rats were assigned to either a sham periodontitis or a periodontitis group. The former group received a daily injection of a vehicle solution (n = 16), while members of the periodontitis induction group (n=16) were injected each day with 500 µL of Pg 108 into the mesial area of the upper molar. Mucin in the submandibular gland was analyzed at the 7th, 14th, 21th and 28th days after injection by means of periodic acid schiff (PAS) staining. Results: 28 days after injection mild gingivitis was developed in the periodontitis experiment group. Junctional epithelium (JE) thickness decreased gradually following the increase of PG injection periods (p<0.05).  However, mucin expression increased prominently at 7th, 14th, and 21th days after injection and decreased on day 28th after PG injection. Mucin was expressed in the duct cells of the submandibular gland. Conclusion: The result of this study suggests that there are different levels of mucin expression in the submandibular gland during periodontitis induction.

EFEKTIVITAS FLEP POSISI KORONAL DENGAN DAN TANPA ACELLULAR DERMAL MATRIX ALLOGRAFT PADA RESESI GINGIVA

B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 1, Nomor 1, Juni 2014
Publisher : Universitas Baiturrahmah

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Abstract

Resesi gingiva adalah terbukanya akar gigi akibat pergeseran posisi margin gingiva ke apikal dari cemento enamel junction. Salah satu perawatan resesi gingiva adalah flep posisi koronal (FPK) yang dapat dilakukan dengan dan tanpa acellular dermal matrix allograft (ADMA). Acellular dermal matrix allograft merupakan jaringan kulit yang diambil dari individu lain yang diproses khusus sehingga sel-sel dermal terangkat dengan tetap mempertahankan matriks kolagen regeneratif. Kandungan utama ADMA terdiri dari kolagen, elastin, proteoglikan dan pembuluh darah yang berguna mengarahkan regenerasi dan reparasi jaringan periodontal. Penelitian ini bertujuan untuk mengetahui efektivitas flep posisi koronal dengan dan tanpa acellular dermal matrix allograft pada perawatan resesi gingiva dilihat dari parameter gingival thickness (GT) yang dinilai dari dua daerah yaitu ketebalan gingiva keratin (GT1) dan ketebalan mukosa alveolar (GT2). Penelitian dilakukan terhadap 20 kasus resesi gingiva dengan melihat pengukuran GT1 dan GT2 dilakukan sebelum dan 3 bulan sesudah operasi. Hasil analisis statistik unpaired t-test menunjukkan adanya perbedaan peningkatan GT1 dan GT2 yang bermakna (p