Ahmad Syaify, Ahmad
Universitas Gadjah Mada, Yogyakarta, Indonesia

Published : 6 Documents
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Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients Syaify, Ahmad; Barunawati, Sri Budi; Suryono, Suryono; HNES, Marsetyawan
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.9986

Abstract

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14)mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RTPCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/MRP14 has role in the regulation of severity periodontitis with diabetes mellitus.
The difference of saline and sterile water for tetracycline hydrochloride solvents in cementum demineralization Ferronika, Shinta; Syaify, Ahmad; Herawati, Dahlia
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 1 (2016): (March 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v49.i1.p5-9

Abstract

Background: The root cementum demineralization is an important step in regenerative periodontal therapy to smear layer removal on the root surface. Smear layer on the root surface becomes a barrier of the new attachment between periodontal tissues with the root surface. The use of tetracycline capsules as root surface demineralizing agent cannot be applied directly on the root surface and solvents such as saline or sterile water are needed. Purpose: The aim of this study was to determine differences between sterile water and saline solvent for tetracycline HCl (tetra HCl) as a cementum demineralization. Method: In this study the specimens were divided into three groups: a control, tetra HCl dissolved in saline, and tetra HCl dissolved in sterile water. Application using burnishing method for 3 minutes. Samples were dehydrated with ethanol series of 30% to 100%. Results of the root demineralization observed by scanning electron microscopy (SEM). Statistical analysis was performed using the Kruskal-Wallis followed by a Mann-Whitney nonparametric test. Result: Upon statistical analysis showed that the sterile water as a solvent of tetra HCl is more effective in smear layer removal and collagen structure exposure in the cementum. Conclusion: Tetra HCl dissolved in sterile water was found to be the best root cementum demineralization agent.
Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ Dewi, Nurdiana; Syaify, Ahmad; Wahyudi, Ivan Arie
Dental Journal (Majalah Kedokteran Gigi) Vol 46, No 3 (2013): (September 2013)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v46.i3.p135-139

Abstract

Background: Amelogenin is a major protein constituent of the developing enamel matrix that is critical for enamel formation. Mutations of amelogenin cause hypoplastic enamel phenotypes. Previous research found that infant of diabetic mother has higher risk for having enamel hypoplasia. Purpose: The aim of this study was to determine the effect of gestational diabetes mellitus on the expression of amelogenin in Wistar rats offspring tooth germ. Methods: Sixteen female Wistar rats, aged 2.5-3 months, body weight 150-200 g were used in this study, Wistar rats were mated and divided into two groups and treated on day 0 of pregnancy. Group A was DM group, consisting of 8 rats, induced by streptozotocin (STZ) injection 40 mg/kg BW. Group B was control group, consisting of 8 rats received citrate buffer injection. Thirty-two rat pups were decapitated on day 5. Immunohistochemical procedures were performed on molar tooth germ of the mandibular rat pups using antibody anti-AMELX to determine the expression of amelogenin. Examination carried out on the images using ImageJ software. All data were then statistically analyzed by Mann Whitney test. Results: There was no significant difference in the expression of amelogenin in the DM group and control group (p>0.05). Conclusion: Gestational diabetes mellitus did not affect the expression of amelogenin in rat offspring tooth germ. Further study is needed to examine the pattern of amelogenin expression with measurement of glucose levels of rat pups.Latar belakang: Amelogenin merupakan protein terbanyak pada matriks email yang berperan penting dalam pembentukan email. Mutasi pada amelogenin dapat menyebabkan email menjadi hipoplastik. Penelitian sebelumnya menunjukkan bahwa anak yang dilahirkan oleh ibu pengidap diabetes memiliki resiko lebih tinggi untuk mengalami hipoplasia email. Tujuan: Penelitian ini bertujuan untuk meneliti pengaruh diabetes mellitus gestasional terhadap ekspresi amelogenin pada benih gigi anak tikus Wistar. Metode: Enam belas ekor tikus Wistar betina, umur 2,5-3 bulan, berat badan 150-200 g digunakan dalam penelitian ini, dikawinkan kemudian dibagi menjadi dua kelompok dan diberi perlakuan pada kehamilan hari ke-0. Kelompok A merupakan kelompok diabetes mellitus, terdiri atas 8 ekor tikus diberi perlakuan diabetes mellitus dengan injeksi streptozotocin (STZ) 40 mg/kg BB. Kelompok B merupakan kelompok kontrol, terdiri atas 8 ekor tikus diberi injeksi buffer sitrat. Tiga puluh dua anak tikus yang lahir didekapitasi pada hari ke- 5. Dilakukan prosedur imunohistokimia pada benih gigi molar rahang bawah anak tikus menggunakan antibodi anti-AMELX untuk mengetahui ekspresi amelogenin. Pemeriksaan dilakukan pada hasil foto menggunakan software ImageJ. Hasil kemudian dianalisa menggunakan uji Mann Whitney. Hasil: Tidak terdapat perbedaan yang bemakna pada ekspresi amelogenin kelompok kontrol dan kelompok diabetes mellitus (p>0,05). Simpulan: Diabetes mellitus gestasional tidak mempengaruhi ekspresi amelogenin pada benih gigi anak tikus. Diperlukan penelitian lebih lanjut untuk mengetahui pola ekspresi amelogenin dengan pengukuran kadar glukosa darah anak tikus.
Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus Syaify, Ahmad; HNES, Marsetyawan; Sudibyo, Sudibyo; Suryono, Suryono
Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 3 (2009): (September 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v42.i3.p130-133

Abstract

Background: Calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils and monocytes/machrophages. This protein is known to be a marker for several inflammatory diseases including periodontitis. In type 2 diabetes mellitus patients, the severity of periodontitis was strongly thought to be caused by decreasing of leukocytes function such as neutrophils. Previous research found that the calprotectin level in serum of periodontitis patients with type 2 DM is higher than periodontits patients non DM. Purpose: The aim of this study was to determine calprotectin mRNA (MRP8/MRP14) expression in human neutrophils of periodontitis patients with type 2 diabetes mellitus. Methods: Neutrophils were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of calprotectin mRNA (MRP8 and MRP14) were detected by RTPCR. Result: The result showed that the value of mRNA calprotectin expression in DM patients were higher than non DM, and the highest expression was on the uncontrolled type 2 DM. Conclusion: The basal level of calprotectin mRNA MRP8/MRP14 expression increased in neutrophil of periodontitis patient with type 2 DM compared non diabetic subjects. It was suggested that high basal level of calprotectin mRNA has a role in the regulation of periodontitis severity with diabetes mellitus patients.
Pengaruh Level HBA1C Terhadap Fungsi Fagositosis Neutrofil (PMN) pada Penderita Periodontitis Diabetika Syaify, Ahmad
Majalah Kedokteran Gigi Indonesia Vol 19, No 2 (2012): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.9375

Abstract

Latar belakang. Diabetes Melitus (DM) merupakan penyakit sistemik yang sangat terkait dengan meningkatnya keparahan penyakit periodontal. Pada penderita DM diduga kuat bahwa keparahan periodontitis disebabkan oleh gangguan fungsi leukosit. Sel neutrofil (PMN) diketahui berperan besar di dalam system pertahanan jaringan periodontal. Kontrol glikemik pada penderita DM dapat diketahui dengan pemeriksaan level HbA1c. Tujuan. Penelitian ini untuk mengetahui pengaruh level HbA1c pada penderita periodontitis diabetika terhadap fungsi fagositosis sel PMN. Bahan dan Cara. Sel PMN dari darah tepi 15 pasien periodontitis terdiri 5 periodontitis tana DM, 5 periodontitis diabetika terkontrol (HbA1c<7) dan 5 periodontitis diabetika tidak terkontrol (HbA1C>7) diuji aktivitas fagositosinya dengan partikel lateks. Fungsi fagositosis dihitung dengan rumus indeks fagositosis (IF). Hasil. Terdapat perbedaan significant IF sel PMN subyek periodontitis diabetika tidak terkontrol (HbA1c>7) dengan periodontitis non DM. IF tertinggi pada subyek periodontitis non DM dan tertinggi pada periodontitis diabetika tidak terkontrol (HbA1c>7). Kesimpulan. Level HbA1c berpengaruh terhadap fungsi fagositosis sel PMN yang dilihat dari indeks fagositosis (IF).  Background. Diabetes mellitus is a systemic condition that has long been associated with an increased and severity of periodontal disease. The severity of periodontitis in diabetic patients was thought caused by decreation of leukocytes function. Polymorphonuclear leukocytes (PMN) play a key role in the maintenance of gingival (HbA1c) level. Aims. The objective of this study was to analyse the influence of HbA1C level to fagositosis index on diabetic periodontitis patients. Material and Methods. PMN were taken from peripheral blood of 15 periodontitis patients consisted of 5 subject with uncontrolled DM (HbA1c>7), 5 subject with controlled DM (HbA1c<7), and 5 subject non DM as control. Phagocytosis function were determined using latex particle and coaunted by phagocytosis index (PI). Results. These analysis revealed a significant (p<0.05) different of PI between diabetic periodontitis who have HbA1c>7 and periodontitis non diabetic subjects. The higest PI was on diabetic periodontitis with HbA1c level >7 and the lowest PI was on non diabetic subjects. Conclusion. Level of HbA1c on diabetic periodontitis patient influence to phagocytosis function of PMN.
EFEKTIVITAS FLEP POSISI KORONAL DENGAN DAN TANPA ACELLULAR DERMAL MATRIX ALLOGRAFT PADA RESESI GINGIVA Lestari, Citra; Murdiastuti, Kwartarini; Syaify, Ahmad
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 1, Nomor 1, Juni 2014
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/JBDjbd.52

Abstract

Resesi gingiva adalah terbukanya akar gigi akibat pergeseran posisi margin gingiva ke apikal dari cemento enamel junction. Salah satu perawatan resesi gingiva adalah flep posisi koronal (FPK) yang dapat dilakukan dengan dan tanpa acellular dermal matrix allograft (ADMA). Acellular dermal matrix allograft merupakan jaringan kulit yang diambil dari individu lain yang diproses khusus sehingga sel-sel dermal terangkat dengan tetap mempertahankan matriks kolagen regeneratif. Kandungan utama ADMA terdiri dari kolagen, elastin, proteoglikan dan pembuluh darah yang berguna mengarahkan regenerasi dan reparasi jaringan periodontal. Penelitian ini bertujuan untuk mengetahui efektivitas flep posisi koronal dengan dan tanpa acellular dermal matrix allograft pada perawatan resesi gingiva dilihat dari parameter gingival thickness (GT) yang dinilai dari dua daerah yaitu ketebalan gingiva keratin (GT1) dan ketebalan mukosa alveolar (GT2). Penelitian dilakukan terhadap 20 kasus resesi gingiva dengan melihat pengukuran GT1 dan GT2 dilakukan sebelum dan 3 bulan sesudah operasi. Hasil analisis statistik unpaired t-test menunjukkan adanya perbedaan peningkatan GT1 dan GT2 yang bermakna (p