Duranta D. Kembaren, Duranta D.
Jurusan Perikanan, Fakultas Pertanian UGM

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ISOLASI DAN KARAKTERISASI BAKTERI PENDENITRIFIKASI YANG DIISOLASI DARI LUMPUR KAWASAN MANGROVE

Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 1 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

The aim of this research is to isolate denitrifying bacteria which have the highest activity to reduce nitrate. The sources of the denitrifying bacteria were mangrove sediment collected from Cilacap Regency, Central Java and Indramayu Regency, West Java. Basalt medium containing KNO3 as a source of nitrogen was used for isolating the denitrifying bacteria. Double layer agar was used for making anaerob condition. Fourty-one isolates were obtained at the first step of the isolation, 29 of them have nitrate reduction activity at a range of 0.77-95.62%. Three isolates, i.e. D19.2, DR2.1 and D27.3 having the highest activity were selected for further examination. The selected isolates were characterized and identified. Characterization includes colony and cell morphology, Gram staining, motility, spore staining and biochemical tests as catalase and oxidase. Identification was done by using profile matching to Bergey’s Manual of Determinative Bacteriology. The results indicate that isolate D19.2 and D27.3 have similarities to the characters of genus Listeria, whereas isolate DR2.1 has similarities to the characters of genus Propionibacterium. All of the selected isolates were able to grow in a medium having NaCl concentration at a range of 0.5-3.5% and pH range of 5-8. Observation of nitrate reduction ability of the isolates during five days incubation shows that isolate DR2.1 has the highest denitrifying activity. The selected isolates can be used as bioremediation agents for controlling nitrate pollution in brackish water pond.

PARAMETER BIOLOGI LOBSTER PASIR (Panulirus homarus) DI PERAIRAN TABANAN, BALI

BAWAL Widya Riset Perikanan Tangkap Vol 7, No 1 (2015): (April 2015)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Penelitian tentang aspek biologi lobster pasir (Panulirus homarus) di perairan Tabanan dilakukan pada periode Maret - Desember 2013. Penelitian ini bertujuan untuk mengetahui aspek biologi lobster pasir meliputi nisbah kelamin, sebaran panjang, kematangan kelamin, hubungan panjang berat dan faktor kondisi. Pengamatan dan pengukuran aspek biologi dilakukan di tempat pengumpul lobster dengan sistem sampling acak. Hasil penelitian menunjukkan bahwa nisbah kelamin lobster pasir berada pada keadaan seimbang. Hasil tangkapan lobster didominasi oleh lobster berusia muda dengan panjang karapas antara 47,5-52,5 mm. Pemijahan terjadi sepanjang tahun dan puncak musim pemijahan lobster pasir diduga terjadi pada bulan Oktober. Persamaan hubungan panjang dan berat lobster jantan adalah W = 0,0021 L2,769 dan betina adalah W= 0,0016 L2,841. Sifat pertumbuhan lobster pasir adalah allometrik negatif. Faktor kondisi relatif rendah pada saat musim pemijahan dan semakin menurun seiring dengan bertambah panjangnya ukuran karapas lobster. Rata-rata ukuran lobster yang tertangkap lebih kecil dari ukuran matang kelamin. Oleh karena itu disarankan untuk menetapkan ukuran minimum yang boleh ditangkap yaitu diatas 68,52 mm.

PARAMETER POPULASI UDANG DOGOL (Metapenaeus ensis) DAN UDANG WINDU (Penaeus monodon) DI LAUT ARAFURA

BAWAL Widya Riset Perikanan Tangkap Vol 9, No 1 (2017): (April, 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Sumber daya udang di perairan Arafura merupakan salah satu modal menuju kemakmuran bagi bangsa, apabila dikelola secara berkelanjutan. Kajian dinamika populasi udang, merupakan salah satu dasar utama dalam merumuskan pengelolaan tersebut menuju pemanfaatan sumber daya yang lestari. Tujuan penelitian ini adalah untuk mengetahui dinamika populasi udang dogol dan udang windu di perairan Arafura dan sekitarnya. Penelitian dilakukan dari bulan Januari 2013 sampai dengan Nopember 2013 dan analisis dilakukan dengan menggunakan FAO-ICLARM Fisheris Stok Assessment Tools (FISAT). Hasil penelitian menunjukkan bahwa laju pertumbuhan (K) udang dogol sebagai 1,33 per tahun dan panjang karapas maksimum (L) sebagai 52,0 mm. Untuk udang windu didapatkan nilai K sebagai 1,45 per tahun dan nilai L sebagai 62,5 mm. Laju kematian total (Z) udang dogol sebagai 4,79 per tahun, laju kematian karena penangkapan (F) dan laju kematian alami (M) masing-masing 2,91 per tahun dan 1,88 per tahun. Untuk udang windu didapatkan nilai Z sebagai 6,13 per tahun, nilai F dan nilai M masing-masing 4,24 dan 1,89 per tahun. Laju pengusahaan (E) udang dogol 0,61 per tahun dan nilai E udang windu sebagai 0,69 per tahun. Tingkat pemanfaatan sudah berada dalam tahapan overfishing dan perlu dilakukan pengurangan jumlah upaya 22% untuk udang dogol dan 38% untuk udang windu.Shrimp resources within Arafura Sea if under sustainably management it would contribute a significant role as a source of nation welfare. Scientific advice on population dynamic are required as an input to support an apropriate fisheries management. The purpose of the study was to identify population parameters of the endeavour shrimp and tiger shrimp in Arafura Sea. Study on the population dynamic of endeavour shrimp and king tiger prawn were conducted in Arafura Sea based on data collected during period of survey, January 2013 to November 2013. The analysis is using the FAO-ICLAM Fisheries Stock Assessment Tools (FiSAT). Result showed that the growth parameter of endeavour shrimp was 1.33/year with maximum carapace length (L) of 52.0 mm. Instantenous total mortality (Z) and natural mortality (M) were 4.79/year and 1.88/year, respectively. While fishing mortality (F) and exploitation rate (E) of endeavour shrimp respectively were 2.91/year and 0.61/year. The growth parameter of king tiger prawn was 1.45/year with maximum carapace length (L) of 62.5 mm. Instantenous total mortality (Z) and natural mortality (M) were 6.13/year and 1.89/year, respectively. While fishing mortality (F) and exploitation rate (E) of king tiger prawn respectively were 4.24/year and 0.69/year. The exploitation rate of endeavour shrimp and king tiger prawn in Arafura Sea was overfishing. It was, therefore, recommended that fishing effort of 22% the endeavour shrimp and 38% king tiger prawn in that waters should be reduced in the next year.

BIOLOGY AND POPULATION DYNAMICS OF BANANA SHRIMP (Penaeus merguiensis) IN THE TARAKAN WATERS, EAST BORNEO

Indonesian Fisheries Research Journal Vol 19, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Study of biology and population dynamic of banana shrimp (Penaeus merguiensis) in Tarakan waters, East Borneo was carried out from January to November 2012. The aim of this research was to identify the biological aspects and population dynamics of banana shrimp. For estimating dynamic population, data were analysed using FiSAT II. The result showed that length at first capture (Lc) of banana shrimp by mini trawl (pukat hela) was 35 mm and the size at first maturity (Lm) was 33,86 mm in carapace length. Spawning occured all year around and reached it’s peak in March. The growth coefficient (K) of banana shrimp was 1,45/year with carapace asymptotic length (CL”) of 80 mm. Total mortality rate (Z) and natural mortality rate (M) were 4,85/year and 1,76/year. While fishing mortality rate (F) and exploitation rate (E) were 3,09/year and 0,64, respectively. The exploitation rate of banana shrimp in Tarakan waters tended to be overexploited so that it needed to be managed wisely and carefully by reducing the fishing effort and fishing season especially on spawning season. The recruitment peak of banana shrimp occured in May.