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Faktor-Faktor yang Berpengaruh terhadap Kejadian Gagal Jantung pada Pasien Infark Miokard Akut di Rumah Sakit dr. Saiful Anwar Malang Baransyah, Livia; Rohman, M. Saifur; Suharsono, Tony
Majalah Kesehatan FKUB Vol 1, No 4 (2014)
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.834 KB)

Abstract

Gagal jantung adalah komplikasi yang umumnya terjadi pada pasien infark miokard akut, dengan insidensinya diperkirakan mencapai 10-40 %. Berbagai faktor berpengaruh terhadap kejadian gagal jantung pada pasien infark miokard akut. Tujuan dari penelitian ini adalah untuk menentukan faktor-faktor yang berpengaruh terhadap kejadian gagal jantung pada pasien infark miokard akut. Sampel dalam penelitian ini berjumlah 65 orang, yang diambil secara consecutive selama 2 bulan di ruang intermediate ward rumah sakit dr. Saiful Anwar Malang. Gagal jantung diklasifikasikan menggunakan kriteria Killip (kelas 1-4). Pasien kriteria ekslusi adalah pasien dengan riwayat gagal jantung. Dalam peneliian ini terdapat 19 orang (29,23 %) dengan gagal jantung (Killip > 1). Berdasarkan hasil uji Chi square dengan tingkat kepercayaan 95 %, didapatkan dislipidemia berpengaruh secara signifikan terhadap kejadian gagal jantung pada pasien infark miokard akut dengan (p = 0,026). Dari 65 orang pasien IMA, kejadian gagal jantung (Killip > 1) terjadi pada 19 orang (29,23 %). Penelitian ini menyimpulkan bahwa faktor yang terbukti berpengaruh terhadap kejadian gagal jantung pada pasien infark miokard akut adalah dislipidemia. Kata kunci: Dislipidemia, Gagal jantung, Infark miokard akut.
Biochemical properties of crude extracellular proteases from Chromohalobacter salexigens BKL5 and Micrococcus luteus 11A Achmad, Ayu Ashari; Rohman, M. Saifur; Prijambada, Irfan D.
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1050.685 KB) | DOI: 10.22146/ijbiotech.26705

Abstract

In this work, we have reported an enzymatic activity and biochemical properties of extracellular proteases from Chromohalobacter salexigens BKL5 and Micrococcus luteus 11A. C. salexigens BKL5 and M. luteus 11A were previously isolated from Bledug Kuwu mud volcano and dietary industry wastewater treatment, respectively. Both bacterial strains were able to produce extracellular proteases, when grown on minimal agar medium supplemented with 1% of skim milk. Proteolytic indexes of C. salexigens BKL5 and M. luteus 11A were 2.5±0.14 and 2.9±0.42, respectively. Both extracellular proteases exhibited optimum enzymatic activity at pH 7, with specific activity of C. salexigens BKL5 was 13.3% higher than that of M. luteus 11A. Optimum temperature for enzymatic activity of both proteases was 45°C. Metal cofactor preferences assay showed that extracellular protease from C. salexigens BKL5 preferred Zn2+, meanwhile extracellular protease from M. luteus 11A mainly preferred Ca2+ ion. Metal cofactor preferences assay also suggested that crude extracellular protease from C. salexigens BKL5 was categorized as metalloprotease, meanwhile crude extracellular protease of M. luteus 11A was common neutral protease. The enzymatic stability assay against various salt concentrations showed that crude extracellular protease from C. salexigens BKL5 was more stable than that of M. luteus 11A.
An Active of Extracellular Cellulose Degrading Enzyme from Termite Bacterial Endosimbiont Rohman, M. Saifur; Pamulatsih, Endang; Kusnadi, Yudi; Yuwono, Triwibowo; Martani, Erni
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.666 KB) | DOI: 10.22146/ijbiotech.15273

Abstract

Cellulase is an ezyme that specifically cleaves the 1,4-β-glycosidic bond of cellulose to produce thesmall fragments of simple carbohydrate. This work was aimed to characterize the extracellular cellulase fromPaenibacillus spp., which was previously isolated from macro termites, Odontotermes bhagwatii in our laboratory.Two Paenibacillus isolates were used in this experiment, namely Paenibacillus cellulositrophicus SBT1 andPaenibacillus, sp. SBT8. Analysis of the total proteins in the supernatants showed that P. cellulositrophicus SBT1and Paenibacillus sp. SBT8 roughly produced as much as 18.6 mg/l and 24.8 mg/l of extracellular cellulases,respectively. Enzymatic assay showed that SBT1 and SBT8 cellulase exhibited enzymatic acitivity of 0.17 U/mg and 0.12 U/mg, respectively. Temperature dependencies analysis indicated that both cellulases exhibitedmaximum activity at 35oC. At the temperature higher than 55oC, the enzymatic activities of both cellulases wereroughly 20% reduced compared to the maximum activity. SBT1 and SBT8 cellulases were both active at acidicpH. At basic pH (pH 8) the enzymatic activities of both cellulases were reduced roughly 30% compared to thatof acidic pH. Supplementing of Mg2+, Zn2+, and Ca2+ in range of 1-10 mM increased the enzymatic activity ofboth cellulases roughly 33 to 50%.