Fitralia Elyza, Fitralia
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IDENTIFIKASI DAN UJI POTENSI BAKTERI LIPOLITIK DARI LIMBAH SBE (SPENT BLEACHING EARTH) SEBAGAI AGEN BIOREMEDIASI

Jurnal Ilmu Lingkungan Vol 13, No 1 (2015): April 2015
Publisher : Program Studi Ilmu Lingkungan,Program Pascasarjana, Universitas Diponegoro

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Abstract

Limbah minyak kelapa sawit yang terbanyak adalah SBE (Spent Bleaching Earth), limbah ini mengandung residu minyak tinggi yang dapat mencemari lingkungan, 30% residu minyak pada limbah SBE dapat digunakan bakteri untuk pertumbuhannya, sehingga adanya bakteri mampu menjadi agen bioremediasi pencemaran SBE. Penelitian dilakukan untuk mendapatkan bakteri lipolitik sebagai agen potensial bioremediasi pada limbah SBE. Metode pengambilan sampel limbah SBE secara random sampling. Sampel tanah diambil secara acak dari beberapa titik area limbah SBE. Bakteri diisolasi dari sampel limbah SBE, kemudian dilakukan tahapan yaitu : pemurnian, seleksi, uji potensi, bakteri berpotensi mereduksi lipid dikarakterisasi dan diidentifikasi genusnya. Hasil penelitian menunjukkan bahwa Aktivitas enzim lipase yang tinggi menandakan bahwa bakteri lipolitik bekerja optimal merombak zat pencemar. Bakteri yang memiliki potensi sebagai agen bioremediasi terdiri dari genus Citrobacter (B1), Enterobacter (B2) dan Acinetobacter (B3). The most palm oil waste is SBE (Spent Bleaching Earth), this waste had many reduced lipid that got pollution for inviroments, Bacteria can use lipid from SBE as much as 30% for growed. So that consist of bacteria in SBE as a potensial agent for remediation. This study aims to obtain lipolytic bacteria as a potential agent of bioremediation. The method of sampling soil were taken at random from SBE waste, Bacteria were isolated from the SBE waste, then they were selected into steps : performed purification, selection, potential test, then characterized and identified it’s genus of potential bacteria. The results showed that the higest activity enzyme of lipolytic indicated that the lipolytic bacteria worked optimal for reduce polution. Bacteria had potential as a bioremediation agent consisting of genus Citrobacter (B1), Enterobacter (B2) and Acinetobacter (B3).

Feasibility of Embung (basin retention) in Green Open Space Area over Institut Teknologi Sumatera (ITERA)

Journal of Multidisciplinary Academic Vol 2, No 1 (2018): Science, Engineering and Social Science Series
Publisher : Penerbit Kemala Indonesia

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Abstract

ITERA has six potential embung (reservoir) as a habitat for fresh water flora and fauna, for place of learning and as water tourism. The function of embung (reservoir) is in accordance with ministry PUPR regulation No. 9/2015 is about using water resources, the embung can be utilized as rain retention basin, and aquaculture activity. Water quality in embung (reservoir) will decrease along with many anthropogenic activites that take place around the embung. This study aims to determine the feasibility of embung water quality and customized with ministry Environmental regulation of water quality management and water pollution control No. 82/2001. Embung (reservoir) in ITERA is belonging to the second class category of water quality management. The purpose of research was to analyze water quality parameters including temperature, phosphate, nitrate, BOD, Turbidity, pH, DO, TSS, and Coliform. There are three embung (reservoir) to be sampled on composite. Embung A is located near the highway, Embung B is located near the north entrace gate of ITERA and Embung C is located near LPPM ITERA building. The output of our research will be a source of data reference for follow up in the identification of water pollution and as a form of water quality management of the embungs.