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Astika Widy Utomo, Astika Widy
Pharmacology and Therapeutic Department of Medical Faculty of Diponegoro University, Semarang
Articles
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Acute Toxicity Test of Soursop Leaves (Annona muricata) on Liver and Kidney of Switzerland Mice

Sains Medika Vol 6, No 2 (2015): July-December 2015
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Introduction: The soursoup leaves extract (Annona muricata) has widely been used as traditional medicine for cancer. No studies have been conduct to investigate the safety of the extract. Objectives: The purpose of the study was to investigate the acute oral toxicity test of soursoup leaves extract (Annona muricata) on Swiss mice’s liver and kidney.Methods: Twenty four mice were divided into 4 groups. Group I was control group, while group II-IV was given soursoup leaves extract as single dose orally via sonde. The mice were obsereved until day 7 to determine the LD50 and at the end were terminated to collect the liver and kidney. The organs later were made into histopathology slides. The slides read with light microscope. The data analyzed with ANOVA and was considered significant at p<0.05.Results: All mice were alive during the 7 days observation and no mice showing the toxic spectrum after the dosing. Microscopically, no damage on the liver and kidney organ among the groups.Conclusion: The LD50 of soursoup leaves extract is more than 2000 mg/kgBW. This result indicate that the extract is practically non toxic and do not damage the liver and kidney.

PENGARUH EKSTRAK KULIT MANGGIS TERHADAP KATALASE ORGAN HEPAR TIKUS TERPAPAR FLUFENAZIN DEKANOAT

JURNAL KEDOKTERAN DIPONEGORO Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Jurusan Kedokteran Umum, Fakultas Kedokteran, Universitas Diponegoro

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Abstract

Latar Belakang : Flufenazin dekanoat merupakan antipsikotik dengan penggunaan jangka panjang. Pengobatan antipsikotik jangka panjang mengakibatkan menurunnya kadar pertahanan antioksidan. Ekstrak kulit manggis memiliki khasiat sebagai antioksidan. Dengan adanya antioksidan pada ekstrak kulit manggis, terjadi penekanan dalam proses pembentukan ROS sehingga tidak menimbulkan stres oksidatif. Enzim katalase digunakan untuk mengetahui stres oksidatif hepar tikus terpapar flufenazin dekanoat diberi ekstrak kulit manggis.Tujuan : Menganalisis pengaruh pemberian ekstrak kulit manggis terhadap kadar katalase organ hepar tikus wistar terpapar flufenazin dekanoat. Metode : Penelitian ini merupakan penelitian eksperimental dengan desain penelitian post test only controlled group design. Sampel adalah 24 ekor tikus wistar dibagi menjadi kelompok kontrol negatif (K0) diberi injeksi sesame oil, kontrol positif (K1) diberi ekstrak kulit manggis ad libitum 600 mg/kgBB, kelompok P1 diberi injeksi fluphenazine decanoat intra muscular, dan kelompok P2 diberi injeksi flufenazin dekanoat intra muscular dan ekstrak kulit manggis ad libitum 600 mg/kgBB. Perlakuan dilakukan selama 28 hari dan dilanjutkan dengan pengukuran kadar katalase hepar. Uji statistik menggunakan uji one way ANOVA dengan post hoc Bonferroni.Hasil  : Dengan menggunakan uji one way ANOVA didapatkan perbedaan signifikan antara kelompok K0 dan K1, K0 dan P2, serta K1 dan P1. Sedangkan antara kelompok K0 dan P1, K1 dan P2, serta P1 dan P2, tidak didapatkan perbedaan yang signifikan.Kesimpulan : Ekstrak kulit manggis menyebabkan peningkatan kadar katalase tikus terpapar flufenazin dekanoat.

KERUSAKAN HEPAR DAN KADAR ENZIM KATALASE TIKUS WISTAR TERPAPAR FLUPHENAZINE DECANOATE

JURNAL KEDOKTERAN DIPONEGORO Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Jurusan Kedokteran Umum, Fakultas Kedokteran, Universitas Diponegoro

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Abstract

Latar belakang : Fluphenazine decanoate merupakan obat anti psikotik golongan tipikal yang dikonsumsi dalam jangka lama. Hal ini dapat menyebabkan peningkatan stress oksidatif. Enzim katalase merupakan salah satu marker stress okisdatif, kerusakan sel akibat stress oksidatif juga dapat dilihat dari histopatologi sel hepar berupa kerusakan ringan hingga kematian sel. Penelitian ini meneliti tentang kerusakan histopatologi hepar dan kadar enzim katalase setelah diberi fluphenazine decanoate.Tujuan : Mengetahui kerusakan histopatologi hepar dan kadar enzim katalase akibat pemberian fluphenazine decanoate dosis bertingkatMetode : Penelitian eksperimental ini menggunakan rancangan post-test only control group design. 15 ekor tikus jantan wistar dibagi 3 kelompok secara acak, yaitu kelompok kontrol (K) diberi diet standar dan injeksi sesame oil, kelompok perlakuan I (P1) diberi diet standar dan injeksi fluphenazine decanoate 1 mg/kgBB, dan kelompok perlakuan PII (P2) diberi diet standar dan injeksi fluphenazine decanoate 2 mg/kgBB selama 28 hari. Hari ke-28 tikus diterminasi dan diambil organ hepar. Sebagian organ hepar dijadikan ekstrak untuk pengukuran kadar katalase dan sebagian dibuat preparat histopatologiHasil : Kadar katalase tertinggi dengan persentase 62,5% dan kerusakan histopatologi terparah dengan skor histopatologi 2,5 didapatkan pada kelompok dengan pemberian fluphenazine decanoate dosis 2 mg/kgBB. Terdapat perbedaan kadar katalase kelompok kontrol dengan kelompok PI walaupun tidak signifikan, sedangkan kelompok lain menunjukkan hubungan yang bermakna.Kesimpulan : Terdapat kerusakan histopatologi hepar dan peningkatan kadar enzim katalase tikus wistar terpapar fluphenazine decanoate dosis 2 mg.

The Effect of Green Tea Leaf Extract on Spatial Memory Function and Superoxyde Dismutase Enzyme Activity in Mice with D-galactose Induced Dimentia

Sains Medika Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Introduction: Oxidative stress and inflammation play an important role in pathogenesis of brain aging and neurodegenerative diseases such as Alzheimer. Green tea has been shown to have antioxidant, anti-inflammatory, anticancer, and neuroprotective activity. Objective: to determine the effect of green tea extract on spatial memory function and superoxide dismutase enzyme activity in mice with D-galactose induced dementiaMethods: An experimental study using “post test only control group design”. Twenty male BALB/c Mice aged 6-8 weeks were divided into 4 groups. Negative control group (NG) was induced by subcutaneous injection of D-galactose (150 mg/kg BW) once daily for 6 weeks. GT-90, GT-270, GT-540 were induced by D-galactose and orally administered with 90, 270, and 540 mg/kg BW of green tea extract once daily for 6 weeks. The spatial memory functions were assessed using Morris water maze and SOD enzyme activities were evaluated using ELISA. One-way Anova and Kruskal-Wallis were used for statistical analysis.Results: mean percentage of latency time in the GT-90 (35.29 (SD= 2.69)%), GT-270 (35.28 (SD= 2.62)%), and GT-540 (35.62 (SD=5.05)%) were significantly higher compared to that of NG (20.38 (SD = 3.21)%), p <0.05). SOD enzyme activity in the GT-270 (0.78 (SD = 0.07) U/ml) was significantly higher compared to that of NG (0.51 (SD = 0.01) U ml), p= 0.004).Conclusion: Green tea extract may improve spatial memory function and the activity of superoxide dismutase enzyme in mice with D-galactose induced dementia.