Aprizal Panus, Aprizal
Institute of Veterinary Subang, FKH IPB

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AEVI-26 INVESTIGASI OUTBREAK SUSPEK BOVINE TUBERCULOSIS (BTB) PADA SAPI PERAH DI PETERNAKAN X DI KABUPATEN BOGOR TAHUN 2018 Juwianto, Tri; Mahpudin, Eka; Panus, Aprizal; Rahmawan, Ali; Sodirun, .
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Bovine Tubercullosis (bTB) adalah penyakit infeksius yang disebabkan oleh Mycobacterium bovis yang ditandai dengan terlihatnya lesi granulomatous (tuberkel) pada paru-paru dan limponodus (Berg et al, 2009). Penyakit ini menyebabkan penurunan produksi dan kerugian ekonomi secara signifikan. Selain itu infeksi penyakit ini juga dapat ditularkan dari hewan ke manusia melalui konsumsi susu atau daging yang terkontaminasi bakteri M. Bovis. Berdasarkan hasil penelitian Halderman (2001) menunjukkan kasus infeksi Tuberculosis (TB) pada manusia di Afrika sebesar 10% disebabkan oleh M. Bovis. Sedangkan di Amerika Latin sebesar 2,5% (Ameni et al, 2007).  Bogor adalah salah satu kabupaten yang ada di Jawa Barat. Kabupaten ini memiliki tipe morofologi wilayah yang bervariasi, dari dataran yang relatif rendah di bagian utara hingga dataran tinggi di bagian selatan. Suhu udara di Kabupaten Bogor rata-rata berkisar antara 22,7O-31,6O C dengan curah hujan yang tinggi. Kondisi geografis dengan suhu yang dingin (agroklimat) sangat sesuai untuk habitat sapi perah. Kabupaten Bogor berkontribusi dalam memenuhi kebutuhan susu nasional melalui pengembangan usaha peternakan sapi perah, diantaranya adalah Kecamatan Cisarua dan Megamendung yang memiliki suhu berkisar 17,85O-23,91OC (rata-rata 20O C). Usaha peternakan sapi perah di daerah tersebut memliki peluang pasar yang cukup besar dan pada saat ini populasi sapi perah di Kabupaten Bogor diperkirakan mencapai kurang lebih 7.000 ekor .                Selama ini prevalensi kasus  bTB pada sapi perah di Kabupaten Bogor belum pernah diketahui dan juga belum pernah dideteksi, sedangkan  laporan kematian sapi perah dengan pemeriksaan menunjukkan perubahan anatomi yang mengarah ke bTB sudah beberapa kali ditemukan. Dikhawatirkan penyakit akan menular dan menyebar ke sapi lain dan juga ke manusia (Radostitset all, 2002). Tuberkulosis zoonotik dapat menjadi ancaman utama bagi kesehatan masyarakat, tetapi belum ada informasi mengenai kejadian penyakit TB zoonotik dan faktor risikonya pada hewan di Indonesia (Daulay, 2015).Investigasi ini dilakukan dalam rangka untuk mengetahui dan mendeteksi kasus suspek bTB pada sapi perah disuatu peternakan di Kabupaten Bogor. Sebanyak 7 kali investigasi dilakukan pada kasus kematian 10 ekor sapi perah dari peternakan X dengan populasi 200 ekor dalam waktu yang berbeda selama periode Pebruari-Agustus 2018.
FA-11 SEROPREVALENCE AND RISK FACTORS FOR BOVINE BRUCELLOSIS IN DAERAH KHUSUS IBUKOTA JAKARTA PROVINCE, INDONESIA Panus, Aprizal; Eakkatat, Monayae; Black, Peter; Indrayani, Liliek; Idris, Safrison
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Brucellosis in dairy cows caused by Brucella abortus is detrimental to the dairy business because it adversely affects the production and reproduction potential of dairy cows.  In Indonesia, annual economic losses due to reproductive disorders including abortion, infertility, sterility, death early of born weak calves and decreased milk production reached 13.8 million US$ (Noor, 2007).Subsequent serological studies have indicated the presence of bovine brucellosis in some areas in Indonesia such as South Sulawesi, West Timor and DKI. Jakarta. Although infection of goats and sheep in Indonesia has not been documented, brucellosis is likely to be present in these species as well. However, brucellosis in these species is generally caused by Brucella melitensis.Brucella abortus is endemic in Indonesia. In past three years, the number of brucellosis cases in DKI. Jakarta has increased with more less 501 heads were detected seropositive recently in 91 farms of dairy cattle and 141 out of 899 samples in 2013, 5 out of 178 in 2014 and 58 out of 202 in 2015 samples were positive Brucellosis reported among approximate 2.550 dairy cattle in DKI. Jakarta province respectively.  Brucellosis was detected not only in dairy cattle, but also in sheep in DKI. Jakarta Province. Therefore brucellosis still a concern of the Indonesian government.Population of cattle in Indonesia is more less 16 million. This population comprises of 15.5 million of beef cattle and 0,5 million of dairy cattle distributed in 33 provinces.  In Indonesia, eradication activities are accompanied by a vaccination program of infected farms conducted to maintain low level of brucellosis at farm level.However the implementation of vaccination program is mostly irregular, and therefore the benefits from the vaccination for brucellosis may be hard to assess. In addition, culling accompanied by a compensation scheme has not worked properly, as the amount of compensation disbursed is generally considered insufficient by farmers. This means that not all seroreactors are culled. (Anka et al, 2014).This situation threatens the cattle population in DKI. Jakarta, especially for DKI. Jakarta Province which has become the source of dairy products such as milk and milk-derived products. Therefore, bovine brucellosis is still one of the targeted diseases that the Indonesian government is trying to eradicate in future. 
Newcastle Disease Virus infection study on duck and chicken in Subang district Panus, Aprizal; Setiyaningsih, S.; Mayasari, N.L.P.I.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.973 KB) | DOI: 10.14334/jitv.v20i2.1168

Abstract

The objectives of this research were to study Newcastle Disease Virus (NDV) infection in Subang area and to examine the diversity of the circulating NDV. Swabs of cloacal and oropharynx, and serum were sampled from total of 393 chickens and 149 ducks in backyard farms and live bird markets located in 10 subdistricts. Screening of NDV in pool of 5-7 samples by real-time Reverse-Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M) showed 19/67 (28.3%) cloacal and 8/67 (11.9%) pharyngeal pools of chicken samples; 18/67 (26.9%) of the pools excreted virus via cloaca and oropharynx, while the duck pools of 8/30 (26.7%) shed virus from cloaca. Virus isolation attempted on individual sample from positive pools yielded 18 isolates which the majority of the isolates showed homogeneous antigenic character, only some of these showed variations up to 2 Log2 with Lasota and 4 Log2 with Komarov antisera. Majority of isolates had a higher affinity to Komarov indicating their propencity to virulent strains. Pathogenicity examination using elution test showed 3 isolates virus were grouped to mesogenic strains and 15 isolates to velogenic strain, in agreement with rRT-PCR fusion results. HI test on 408 sera showed that NDV antibody was detected in 48 (12%) birds with titres ranging from 1 to 8 Log2; only about 13% of vaccinated chickens demonstrated protective antibody titre (≥3 Log2). Newcastle disease is still endemic in Subang with relatively low antigenic variation among circulating strains. Key Words: Newcastle Disease, rRT-PCR Detection, Detection of Virulence, Antigenic Diversity, Antibody
Newcastle Disease Virus infection study on duck and chicken in Subang district Panus, Aprizal; Setiyaningsih, S.; Mayasari, N.L.P.I.
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.973 KB) | DOI: 10.14334/jitv.v20i2.1168

Abstract

The objectives of this research were to study Newcastle Disease Virus (NDV) infection in Subang area and to examine the diversity of the circulating NDV. Swabs of cloacal and oropharynx, and serum were sampled from total of 393 chickens and 149 ducks in backyard farms and live bird markets located in 10 subdistricts. Screening of NDV in pool of 5-7 samples by real-time Reverse-Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M) showed 19/67 (28.3%) cloacal and 8/67 (11.9%) pharyngeal pools of chicken samples; 18/67 (26.9%) of the pools excreted virus via cloaca and oropharynx, while the duck pools of 8/30 (26.7%) shed virus from cloaca. Virus isolation attempted on individual sample from positive pools yielded 18 isolates which the majority of the isolates showed homogeneous antigenic character, only some of these showed variations up to 2 Log2 with Lasota and 4 Log2 with Komarov antisera. Majority of isolates had a higher affinity to Komarov indicating their propencity to virulent strains. Pathogenicity examination using elution test showed 3 isolates virus were grouped to mesogenic strains and 15 isolates to velogenic strain, in agreement with rRT-PCR fusion results. HI test on 408 sera showed that NDV antibody was detected in 48 (12%) birds with titres ranging from 1 to 8 Log2; only about 13% of vaccinated chickens demonstrated protective antibody titre (≥3 Log2). Newcastle disease is still endemic in Subang with relatively low antigenic variation among circulating strains.