Nurasiah Djaenuddin, Nurasiah
Balai Penelitian Tanaman Serealia Jl. Dr. Ratulangi No. 274, Maros, Sulawesi Selatan

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Uji Keefektifan Formulasi Bacillus subtilis untuk Mengendalikan Penyakit Busuk Batang Fusarium pada Tanaman Jagung In Vivo Suriani, Suriani; Djaenuddin, Nurasiah; Muis, Amran
Jurnal Penelitian Pertanian Tanaman Pangan Vol 2, No 3 (2018): DESEMBER 2018
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v2n3.2018.p191-197

Abstract

Stalk rot diseases in corn caused by the fungus Fusarium verticilloides is one of the problems of increasing maize productivity. This pathogen can be transmitted through seed and soil. The method of control through seedtreatment with biological control is one of an effective control method of the soilborne pathogen. This research aimed to study the effectiveness B. subtilis formulation in controlling stalk rot disease.  The experiment was conducted in the plant Pathology Laboratory and in the greenhouse of Indonesia Cereals Research Institute (ICERI) from April 2017 to January 2018. The treatment used 8 formulation of B. subtilis of ICERI collection, synthetic fungicide and positive control. In vitro test was inhibition 8 formulation of B. subtilis to F. verticilloides growth by dual culture method. While activity in the green house is to test of effectiviness 8 formulation of B. subtilis in controlling the stalk rot. Seedtreatment of B. subtilis formulation TLB1, BS-BJ6, BS-M3, BS-TM4, BS-BNt4, BS-BNt5, BS-BNt6, BS-BNt8 and two controls (K1 with the use of synthetic fungicides and K2 with steril destiled water). Data collected were percentage inhibition of the growth of F. verticilloides miselia, disease incidence, and plant height. The results showed that formulation of B. subtilis BNt4 performed the best to inhibited growh of  the F. verticilloides in vitro test. Application of  B. subtilis  formulations BNt4 and TM3 by means of seed treatment can suppress stalk rot attack. The all of B. subtilis  formulations can stimulate plant growth.
SCREEN HOUSE TEST OF EIGHT BIOPESTICIDE FORMULATION BACILLUS SUBTILIS AGAINST DOWNY MILDEW, PERONOSCLEROSPORA PHILIPINENSIS, ON CORN PLANT Djaenuddin, Nurasiah; Muis, Amran; Nonci, Nurnina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.139 KB) | DOI: 10.23960/j.hptt.11851-58

Abstract

Screen House Test of Eight Biopesticide Formulation Bacillus subtilis against Downy Mildew, Peronosclerospora philipinensis, on Corn Plant. Corn downy mildew (DM) caused by Peronosclerospora spp. is a major constraint to the decline in maize production because it can cause yield loss up to 100%. So far, DM was successfully controlled with metalxyl fungicide. But lately, the effectiveness of this fungicide has begun to decline, so there is a need for an appropriate and environmentally friendly control alternative, one of which is the use of biological control agents. The aim of this study was to know the influence of eight types of biopesticide formulation of Bacillus subtilis in controlling DM of corn plant. The study was conducted at the screenhouse of the Indonesian Cereals Research Institute at Maros from November to December 2016. The treatments were arranged in a randomized block design with nine treatments and three replications. The treatment consisted of seed treatment with eight types of B. subtilis formulations (TM4, TM3, BNt4, BNt5, BNt6, BNt8, BJ6, and TLB1) and one treatment with sterile water (control). The variety used was Anoman which is known to be highly susceptible to DM. The variables observed included number of germination, the incidence and incubation period of disease, plant height, and dry weight of the plant. The results showed that the eight B. subtilis formulations had the potential to suppress DM in corn. The symptom of DM began to be seen at 18 days after planting (DAP) on the treatment of BNt8, while the controls have been seen at 12 HST. B. subtilis TM formulation has the highest potential to suppress DM with the percentage of disease suppression reaching 63.1%.
EFEKTIVITAS FORMULA BACILLUS SUBTILIS TM4 UNTUK PENGENDALIAN PENYAKIT PADA TANAMAN JAGUNG Djaenuddin, Nurasiah; Nonci, Nurnina; Muis, Amran
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.15 KB) | DOI: 10.14692/jfi.13.4.113

Abstract

Banded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize.   The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB .Key words: Bacillus subtilis, biopesticide, Bipolaris maydis, leaf blight diseaseBanded leaf and sheath blight (BLSB) and maydis leaf blight (MLB) caused by Rhizoctonia solani and Bipolaris maydis, respectively are considered as important diseases in maize.   The use of biopesticides is an alternative method to control the diseases. This study was conducted to determine the effectiveness of bacterial formula Bacillus subtilis to inhibit the development of BLSB and MLB on the plant. Testing of biopesticide formula was done in two different applications, i.e. seed treatment for BLSB control and leaf spraying in the field for MLB. The results showed that the B.subtilis formula effectively suppressed the development of BLSB but it was not effectively suppressed the development of MLB.
EVALUASI LIMA JENIS INNER CARRIER DAN FORMULASI BACILLUS SUBTILIS UNTUK PENGENDALIAN HAWAR PELEPAH JAGUNG (RHIZOCTONIA SOLANI KUHN) Muis, Amran; Djaenuddin, Nurasiah; Nonci, Nurnina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 15, No 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.614 KB) | DOI: 10.23960/j.hptt.215164-169

Abstract

Evaluation of five inner carriers and Bacillus subtilis formulation to control banded leaf and sheath blight (Rhizoctonia solani Kuhn). One alternative control method against plant pathogens is the use of antagonistic microorganisms, such as Bacillus subtilis. The use of the antagonistic bacteria on corn especially in Indonesia is still lack. The objective of this research was to evaluate some inner carrier and to make formulated antagonistic B. subtilis to be used as biological control agents on corn diseases. This research consists of laboratory and greenhouse activities. The laboratory activities consist of B. subtilis biomass production, formulation of B. subtilis, and evaluation of five types of inner carrier. In the greenhouse, testing the formulation B. subtilis with talc as an inner carrier, which is compared with the treatment solution of B. subtilis, nordox, metalaxyl fungicides. The data collected in this study were percentage of germination, damping off due to pathogen R. solani, plant height, plant fresh weight, and percentages of R. solani incidence on 14 DAP. The results showed that talc powder and corn flour were the best inner carrier to be used in sorage formulation of antagonistic Bacillus. Formulated Bacillus subtilis TM4 showed no negative affect on seed germination and able to suppress the development of R. solani in greenhouse.
EFEKTIVITAS BIOPESTISIDA BACILLUS SUBTILIS BNt 8 DAN PESTISIDA NABATI UNTUK PENGENDALIAN PENYAKIT HAWAR PELEPAH DAN UPIH DAUN JAGUNG Djaenuddin, Nurasiah; Muis, Amran
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.944 KB) | DOI: 10.23960/j.hptt.11753-61

Abstract

Effectiveness of the biopesticide of Bacillus subtilis BNt 8 and botanical pesticide in controlling banded leaf and sheath blight disease on maize. Banded leaf and sheath blight disease (BLSB) caused by the fungus Rhizoctonia solani is difficult to control because it pertained soil borne fungus that can survive in a long time in the soil. Control the disease with synthetic pesticide causing contamination to the environment, so that an environmentally friendly alternative control is needed. This study aimed to obtain a Bacillus subtilis formulation as biological agents and selected botanical pesticides that effective to control BLSB in the field. The study was conducted at the Plant Pathology Laboratory of Indonesia Cereals Research Institutein Maros and at the Bajeng Experimental Farm in Gowa, held from February to August 2015. The reatments consists of several botanical pesticides, B. subtilis formulation, a synthetic fungicide, positive and negative controls. In vitro test was inhibition test between botanical pesticide with R. solani and antagonistic test between the B. subtilis and botanical pesticides, each of them consists of 6 treatments and 3 replications, while the field activity consists of test of effectiveness of single treatment and combination between B. subtilis formulation and botanical pesticides. The results showed that combination of formulated B. subtilis with botanical pesticide of cloves leaves, betel leaves, and turmeric were not significantly different from single treatment of formulated B. subtilis and botanical pesticides. Formulated B. subtilis suppressed the severity of BLSB as much as 39.1% and yield reached 8.4 t/ha.
Isolasi dan Uji Efektivitas Beberapa Isolat Dekomposer Lokal dalam Mendekomposisi Limbah Tanaman Jagung Djaenuddin, Nurasiah; Faesal, Faesal; Soenartiningsih, Soenartiningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.213

Abstract

Soil microorganisms are able to digest and degrade organic material present in the environment in to substances that available for plants to absorb. This research aimed to isolate and examine local bacteria that had the ability to decompose corn stover. Soil samples were taken from various natural habitats in which bacteria grow, including remains of corn planting area, pile of waste corncob and corn stalk, pile of rice husk and straw, and pile of used sorghum stalk. Which were collected from several locations across South Sulawesi. Bacteria was isolated by making soil suspension that being inoculated on to NA and CMC medium, and then incubated for 48 hours at room temperature. Bacterial colonies that grow were then incubated 50o C to obtain bacteria resistant to high temperature and able to produce cellulose. Bacterial isolates that produce cellulose was then examined for the effectiveness in decomposing corn stover waste. Isolates result were able to obtain 119 decomposers isolates with 29 isolates resistant to temperature above 50o C and had ability to produce cellulose. From 29 isolates, among 15 isolates collected from rice straw, 5 isolates from remains of corn planting area and 9 isolates collected from several natural habitats. Almost all isolates had good ability to decompose corn stover waste. From isolates tested in corn cob waste and corn leaves waste, only 16 bacterial isolates were proven to be effective as decomposer.
CORRELATION OF STOMATA DENSITY TO RUST SEVERITY ON SOME ACCESSIONS OF MAIZE GERMPLASM ., Suriani; Djaenuddin, Nurasiah; Talanca, A.Haris
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 2 (2018): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.218%p

Abstract

Correlation of stomata density to rust severity on some accessions of maize germplasm. Rust is an important disease on maize. Control of rust using resistant varieties is recommended because it is more practical and environmental friendly. This research aims to study on the correlation of stomata density to rust severity on the several maize germplasm accessions. The research was conducted in Bontobili Farm Experimental Station and ICERI Pathology Laboratory, held from March to August 2017; it used Randomized Block Design with 2 replicates. As much as 30 corn germplasm accessions and 2 varieties for comparison (Bima 10 and Anoman) were planted by spacing 20 x 70 cm. The inoculation of Puccinia sp. was allowed to occur naturally. Printing of stomata was conducted with painted the lower part of corn leaf with nail polish transparent. After drying, it was pasted with clear isolation and then it was pulled and saved on glass object. Observed parameters were rust severity on the age of 50, 60, 70 days after planting and density of stomata. Results of the experiment showed that disease severity of germplasm accession number 2, 218 and 243 were not significant with comparison (tolerant variety). Germplasm with accession number 234 was categorized tolerant to rust. The rust infection rate on all accession were categorized by mild to moderate. Density of stomata of each maize germplasm accession was significantly correlated with rust severity, the increase of stomata density could increase of rust severity at 0.73%.
THE EFFECTIVENESS COMBINATION OF RESISTANT VARIETIES AND METALAXIL FUNGICIDE IN CONTROLLING DOWNY MILDEW DISEASE (Peronosclerospora maydis) IN MAIZE PLANT Pakki, Syahrir; Djaenuddin, Nurasiah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 19, No 1 (2019): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.728 KB) | DOI: 10.23960/j.hptt.119%p

Abstract

Downy mildew caused by Peronosclerospora maydis is an important disease in the centers of corn cultivation in Java. The aim of this study was to determine the effectiveness of a combination of metalaxyl fungicide and varieties that have a high sustainability of downy mildew caused by P. maydis. The study was conducted in Kediri, East Java, which is an endemic area of downy mildew caused by P. maydis. The Split Plot Design with 3 replications was used in this study. The main plots were 5 corn varieties (1) Bima-3 Bantimurung, (2) Bima-20 URI, (3) Lagaligo, (4) Bima-15 Sayang, and (5) Anoman as a susceptible check. The subplots were 5 levels of seed treatment dose with metalaxyl fungicide (0 g/kg, 2 g/kg, 3 g/kg, 5 g/kg, and 7 g/kg seeds). The combination of resistant varieties with metalaxyl at a dose level of 5 g and 7 g/kg of corn seeds was effective in controlling downy mildew caused by P. maydis. In Bima-3 varieties Bantimurung and Lagaligo showed low infection reactions of 7.7-8.1%, and 10.4?11.2% respectively. In a combination of treatment conditions of susceptible varieties (Anoman) with 2, 3, 5 and 7 g/kg seeds, disease incidence reach 100% or most of the plants die. The lower incidence was also followed by yield, ear length and weight of a 1000 seeds that higher than other treatments. The combination of the use of susceptible variety with 2 g to 7 g/kg of metallaxyl doses was not effective in controlling downy mildew caused by P. maydis.