Mamik Setyowati, Mamik
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Ragunan 29, Pasar Minggu, Jakarta Selatan 12540, Indonesia

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PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN SORGUM (Sorghum bicolor L. Moench) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Widyasari, Annisaa; Wijanarka, W; Raharjo, Budi; Setyowati, Mamik
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sorghum was a cereal crop that has many benefit such as food, feed, industrial, and bioenergy. Sorghum had a potency to be cultivated, but productivity of sorghum was still low both in quatity and quality. One way to increase production of sorghum  is using rhizobacteria as biofertilizer. The aim of this study is to get rhizobacteria that has the ability to produce IAA, solubility of phospat (P), Nitrogen (N) fixing, and analyze the effect of rhizobacteria inoculants for enhance sorgum plant growth. Isolation of rhizobacteria was done by diluting  rhizobacteria sorghum suspension from 10-1 to 10-5 and it were be platted on SEA medium. Isolates were screened by ability to produce IAA, solubility of P, and N fixing. Producing of IAA test was done by adding Salkowsky reagent on bacterial supernatant and measured absorbance at 530 nm wavelength. Solubility of P test was done by inoculating isolates in Pikovskaya media, while N fixing test was done on N fixing media (NFB). Isolates of rhizobacteria which had a potency to increase growth of plants were made inoculants to be applied in sorghum plants. The result of this study obtain 3 isolates i.e Sr 194.3; Sr 172.1; and Sr 209.1 which were considered effective for increase growth of sorghum. The conclusion  of this study isolates which showed the highest average plant height, root length, and dry weight Sr 194.3 isolate. The statistical analysis among the treatments showed that did not any significant differences on plant height, root length, and dry weight of sorghum age 28 days after farming. Keyword : Increase growth plants, Screening, Shorgum, Rhizophere.
Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Susilowati, Dwi Ningsih; Fauziah, Fani; Pranoto, Eko; Hidayat, Ernin; Setyowati, Mamik; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
HUBUNGAN KARAKTER FENOTIPIK DAN HASIL BIJI PLASMA NUTFAH KACANG TUNGGAK [Vigna unguiculata (L.) Walp.] MENURUT ANALISIS LINTASAN Mastur, Mastur; Setyowati, Mamik; Susilowati, Dwi N.
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Selection of high quality seedling through plant breeding activity can be conducted using yielded seeds, but usually it produces a low heritability. Therefore, this study was aimed to examine and analize several secondary phenotypic characters of 115 cowpea germ plasms in relation to seed yield. Relationship between secondary characters and seed yield was analyzed using path analysis. The result of path analysis showed that the number of cluster and the number of pod exhibited direct and significant effect to the seed yield of cowpea. Therefore, number of cluster and number of pod are necessary to be included as the selection criteria for breeding programme to produce high quality cowpea seedling with high yield.
ANALISIS AKTIVITAS NITROGENASE DAN GEN NIFH ISOLAT BAKTERI RHIZOSFER TANAMAN PADI DARI LAHAN SAWAH PESISIR JAWA BARAT Susilowati, Dwi Ningsih; Setyowati, Mamik
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology

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Abstract

AbstrakPenambatan nitrogen oleh bakteri rhizosfer dapat dimanfaatkan untuk menyiasati dampak salinitas pada tanah sawah pesisir. Kemampuan tersebut disebabkan oleh aktivitas nitrogenase yang disandikan gen nifH pada komponen II. Penelitian  ini bertujuan  menganalisis aktivitas nitrogenase pada kondisi salin dan mengidentifikasi gen nifH. Sebanyak 50 isolat bakteri rhizosfer asal tanah sawah pesisir daerah Eretan dan Patimban, Jawa Barat telah dianalisis. Lima isolat yang menunjukkan aktivitas nitrogenase pada kondisi salin adalah Er B1 3, Er B1 4, Er B1 9, Er B2 10, dan Ptb B1 4. Gen nifH kelima sampel diidentifikasi menggunakan PCR menghasilkan amplikon berukuran ~360 bp. Aktivitas nitrogenase tertinggi berdasarkan Analisis Reduksi Asetilen (ARA) diperoleh pada isolat Er B2 10 yang memiliki kekerabatan terdekat dengan bakteri Providencia sp. Hasil yang diperoleh membuktikan bahwa beberapa bakteri asal sawah pesisir dapat menambat nitrogen pada kondisi salin.AbstractThe ability of nitrogen fixation by rhizosphere bacteria could be used to decrease salinity impact in coastal paddy field, due to nitrogenase capability, encoded by a nifH gene in component II. The objectives of this research are to analyze nitrogenase activity in saline condition and identify the presense of the nifH gene. A total of 50 isolates of the rhizosphere bacteria coastal from wetland areas of Eretan and Patimban, West Java, has been isolated and being analyzed. Among them, five isolates i.e. Er B1 3, ER B1 4, Er B1 9, Er B2 10 and Ptb B1 4, showed the nitrogenase activity under saline condition. The polymerase chain reaction (PCR) of the nifH gene from those five samples resulted in the amplicon size of  ~360 bp. The highest activity of nitrogenase assessed by acetylene reduction assay (ARA) was shown by Er B2 10 which closely related to bacteria of Providencia sp. The obtained result showed that several bacteria from coastal paddy field were able to conduct nitrogen fixation under saline stress.
Penerapan Metode Investigasi Kelompok Untuk Meningkatkan Sikap Solidaritas Sosial Dan Keterampilan Komunikasi Siswa Kelas V SDN Selosari 2 Magetan Semester Gasal Tahun Pelajaran 2017/2018 Setyowati, Mamik
Gulawentah:Jurnal Studi Sosial Vol 3, No 1 (2018)
Publisher : Universitas PGRI Madiun

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Abstract

Penelitian ini bertujuan untuk meningkatkan sikap solidaritas sosial dan keterampilan komunikasi siswa kelas V SDN Selosari 2 Magetan Semester Gasal Tahun Pelajaran 2017/2018 dengan menerapkan investigasi kelompok. Ada beberapa penyebab rendahnya sikap solidaritas sosial dan keterampilan komunikasi IPS, diantaranya karena penggunaan mengajar selama ini masih sangat monoton yang didominasi ceramah sehingga aktivitas anak kurang nampak. Maka menjadi suatu tantangan bagi seorang guru untuk senantiasa melakukan inovasi dalam menggunakan mengajar. Subyek penelitian yang digunakan oleh peneliti adalah kelas V SDN Selosari 2 sebanyak 25 anak. Pengumpulan data dalam penelitian ini menggunakan wawancara dan observasi. Dalam Penelitian Tindakan Kelas ini peneliti bekerja sama dengan salah seorang guru sebagai kolaborator sekaligus observer dalam mengamati aktivitas belajar siswa. Penerapan investasi kelompok dapat meningkatkan keterampilan komunikasi dan sikap solidaritas siswa kelas V SDN Selosari 2. Aspek keterampilan komunikasi terjadi kenaikan pada siklus 2 daripada siklus 1. Nilai rata-rata pada siklus 1 mencapai 74,5, sedangkan pada siklus 2 naik menjadi 93. Sedangkan aspek sikap solidaritas sosial siswa selama kegiatan belajar mengajar juga mengalami kenaikan. Siklus 1 mendapatkan rata-rata 75,5, sedangkan pada siklus 2 mencapai 90,5. 
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN JAGUNG (Zea mays) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Widiyanti, Debby; Pujiyanto, Sri; Suprihadi, Agung; Setyowati, Mamik
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Maize (Zea mays) is the second most important food commodity after rice plants. The role of the corn crop in agricultural commodities encourage the use of biological fertilizers to increase crop productivity of maize. The use of biological fertilizers open opportunities using rhizosphere bacteria as inoculants boosters grow plants.This study aims to obtain bacterial isolates from the rhizosphere of maize (Zea mays), characterize the morphology and biochemical properties of the ability to produce IAA, provide nutrients such as N and P and to get a bacterial inoculant boosters grow corn. Rhizosphere bacteria were isolated using SEA media (soil extract agar). Isolates that have the ability to produce IAA, tether N and high P solvents tested against corn seed germination and applied in a phase of plant growth. The results showed there were 24 isolates corn crop rhizosphere dominated by gram-positive bacteria and shaped basil capable of producing IAA grading 4,83 to 125,84 ppm. 16 rhizosphere bacterial isolates capable of dissolving phosphate with phosphate solubility index ranged from 2,1 to 4,6. 18 rhizosphere bacterial isolates capable of tether N with the highest N tether capacity of 2,8 x 10 3 CFU/ mL. Application 3 selected bacteria such as J11, J16 and J19 as inoculants are not able to increase the growth of corn plants compared to control water. Keyword :Maize, Rhizosphere, The growth of corn plants.