Anita Yuliati, Anita
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Application of chitosan scaffolds on vascular endothelial growth factor and fibroblast growth factor 2 expressions in tissue engineering principles Pratiwi, Ariyati Retno; Yuliati, Anita; Soepribadi, Istiati; Ariani, Maretaningtias Dwi
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 4 (2015): (December 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.674 KB) | DOI: 10.20473/j.djmkg.v48.i4.p213-216

Abstract

Background: Tissue engineering has given satisfactory results as biological tissue substitutes to restore, replace, or regenerate tissues that have a defect. Chitosan is an organic biomaterial often used in the biomedical field. Chitosan has biocompatible, antifungal, and antibacterial properties. Chitosan is osteoconductive, suitable for bone regeneration applications. Bone defect healing begins with inflammatory phase as a response to the presence of vascular injury, so new vascularization is required. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor-2 (FGF2) are indicators of the beginning of bone regeneration process, playing an important role in angiogenesis. Purpose: This research was aimed to determine the effects of chitosan scaffold application on the expressions of VEGF and FGF2 in tissue engineering principles. Method: Chitosan was dissolved in CH3COOH and NaOH to form a gel. Chitosan gel was then printed in mould to freeze dry for 24 hours. Those rats with defected bones were divided into two groups. Group 1 was the control group which defected bones were not administrated with chitosan scaffolds. Group 2 was the treatment group which defected bones were administrated with chitosan scaffolds. Those rats were sacrificed on day 14. Tissue preparations were made, and then immunohistochemical staining was conducted. Finally, a statistical analysis was conducted using Kruskal Wallis test. Result: There was no significant difference in the expressions of VEGF and FGF2 between the control group and the treatment group (p>0.05). Conclusion: Chitosan scaffolds do not affect the expressions of VEGF and FGF2 during bone regeneration process on day 14 in tissue engineering principles
Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials Sularsih, Sularsih; Yuliati, Anita; D, Coen Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol 45, No 1 (2012): (March 2012)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.046 KB) | DOI: 10.20473/j.djmkg.v45.i1.p17-21

Abstract

Background: Chitosan is biomaterial improved for various dentistry applications because it is biocompatible, degradable, nontoxic, and not carcinogenic. The main parameter affecting the characteristics of chitosan is deacetylation degree. Purpose: This study is aimed to determine the degree of deacetylated of chitosan derived from white shrimp shell waste used as dental biomaterial. Methods: White shrimp shells were crushed into powder. Next, deproteination process was conducted with 3.5% NaOH solution, demineralized with 1N HCl solution, and then depigmented with 90% acetone solution into chitin powder. Deacetylation process was then conducted by soaking the chitin powder in 50% NaOH solution for 6 h at 65° C to produce white powder of chitosan. Afterwards, deacetylation degree test was conducted by using Fourier Transform Infrared Spectrophotometer (FTIR) to calculate the ratio of the absorption bands between the absorbance peak of amide group about 1655 cm–1 and the absorbance peak of hydroxyl group about 3450 cm–1. Results: The result of the deacetylation degree test on the chitosan powder derived from white shrimp shell waste was high, about 85.165%, and had the eligible form, solubility, and pH. Conclusion: It can be concluded that the deacetylation degree of chitosan from white shrimp shells could reach 85.165%.Latar belakang: Kitosan merupakan biomaterial yang dikembangkan untuk berbagai aplikasi kedokteran gigi karena biokompatibel, dapat didegradasi, tidak toksik dan tidak karsinogenik. Parameter utama yang mempengaruhi karakteristik kitosan adalah derajat deasetilasi. Tujuan: Tujuan dari penelitian ini adalah mengetahui derajat deasetilasi kitosan dari limbah kulit udang putih sebagai biomaterial kedokteran gigi. Metode: Kulit udang putih dihaluskan menjadi serbuk. Setelah itu dilakukan proses deproteinasi dengan larutan NaOH 3,5%, demineralisasi dengan larutan HCl 1N, depigmentasi dengan larutan aseton 90% sehingga menjadi serbuk kitin. Proses deasetilasi dilakukan dengan merendam serbuk kitin dalam larutan NaOH 50% selama 6 jam pada suhu 65° C sehingga dihasilkan serbuk putih kitosan. Uji derajat deasetilisasi menggunakan metode spektrofotometer Fourier Transform Inframerah (FTIR) dengan menghitung nilai perbandingan pita serapan antara puncak absorbansi gugus amida sekitar 1655 cm–1, dan puncak absorbansi gugus hidroksil sekitar 3450 cm–1. Hasil: Hasil uji derajat deasetilasi serbuk kitosan dari limbah kulit udang putih adalah tinggi yaitu sebesar 85.165% dan memiliki bentuk, kelarutan dan pH yang memenuhi syarat. Kesimpulan: Dapat disimpulkan derajat deasitilasi kitosan dari kulit udang putih adalah 85,165%.
In vitro effect of Q-switched Nd:YAG laser exposure on morphology, hydroxyapatite composition and microhardness properties of human dentin Apsari, Retna; Siswanto, Siswanto; Yuliati, Anita; Bidin, Noriah
Dental Journal (Majalah Kedokteran Gigi) Vol 44, No 4 (2011): (December 2011)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.266 KB) | DOI: 10.20473/j.djmkg.v44.i4.p181-186

Abstract

Background: A Q-switched Nd:YAG laser was employed as a source of ablation. The fundamental wavelength of the laser is 1064 nm, with pulse duration of 8 nanosecond operates with uniphase mode of TEM00. In the following experiments, dentin samples (without caries and plaque) are exposed to pulse laser with Q-switching effect at various energy dose. Purpose: The aim of this study was to investigate the effect of laser ablation on dentin samples using Q-switched Nd:YAG laser exposure. Methods: The laser was operated in repetitive mode with frequency of 10 Hz. The energy dose of the laser was ranging from 13.9 J/cm2, 21.2 J/cm2 and 41.7 J/cm2. The target material comprised of human dentin. The laser was exposed in one mode with Q-switched Nd:YAG laser. Energy delivered to the target through free beam technique. The exposed human dentin was examined by using x-ray diffraction (XRD) and fluoresence scanning electron microscopy for energy dispersive (FESEM-EDAX). Microhardness of human dentin were examined by using microhardness vickers test (MVT). Results: The result obtained showed that the composition of hydroxyapatite of the dentin after exposed by Q-switched Nd:YAG laser are 75.02% to 78.21%, with microhardness of 38.7 kgf/mm2 to 86.6 kgf/mm2. This indicated that exposed pulsed Nd:YAG laser on the human dentin attributed to the phototermal effect. The power density created by the Q-switched Nd:YAG laser enables the heat to produce optical breakdown (melting and hole) associated with plasma formation and shock wave propagation, from energy dose of 21.2 J/cm2. From XRD analysis showed that the exposure of Nd:YAG laser did not involve in changing the crystal structure of the dentin, but due to photoablation effect. Conclusion: In conclusion, the application of Q-switched Nd:YAG laser as contactless drills in dentistry should be regarded as an alternative to the classical mechanical technique to improve the quality of the dentin treatment.Latar belakang: Mode Q-Switch pada laser Nd:YAG dapat menghasilkan fenomena ablasi pada dentin. Laser Nd:YAG yang digunakan mempunyai panjang gelombang 1064 nm, durasi pulsa 8 ns beroperasi dengan mode TEM00. Sampel dentin yang digunakan tanpa karies dan plak, yang dipapari laser dengan mode Q-switch dalam berbagai variasi dosis energi. Tujuan: Tujuan penelitian ini adalah mengamati efek ablasi dentin secara in vitro akibat paparan laser Nd:YAG Q-switch dengan pengamatan morfologi permukaan, komposisi hidroksiapatit, dan uji kekerasan mikro. Metode: Laser Nd:YAG Q-switch dengan frekuensi 10 Hz dan variasi dosis energi 13,9 J/cm2, 21,2 J/cm2 dan 41,7 J/cm2 ditembakkan pada sampel dentin manusia dengan teknik penyinaran bebas tanpa dilewatkan serat optik. Dentin yang terbuka diamati menggunakan x-ray diffraction (XRD) dan fluoresence scanning electron microscopy for energy dispersive (FESEM-EDAX). Kekerasan mikro dari dentin juga diamati menggunakan microhardness vickers test (MVT). Hasil: Hasil penelitian menunjukkan bahwa komposisi hidroksiapatit dari dentin setelah paparan laser Nd:YAG Q-Switch menunjukkan peningkatan berkisar 75,02% sampai 78,21% dibandingkan normal, dengan kekerasan mikro berkisar 38,7 kgf/mm2 sampai 86,6 kgf/mm2. Perubahan pada struktur mikro tersebut disebakan karena adanya efek fototermal. Kerapatan daya yang bervariasi berdasarkan variasi dosis energi menyebabkan efek panas pada dentin yang menyebabkan adanya fenomena optical breakdown, yang ditandai dengan munculnya efek leleh dan lubang pada sampel karena produksi plasma dan adanya gelombang kejut, mulai dosis 21,2 J/cm2. Berdasarkan uji XRD, efek yang muncul pada dentin tidak menyebabkan terjadinya perubahan struktur kristal hidroksiapatit, tetapi menyebabkan perubahan komposisi hidroksiapatit yang disebut dengan fotoablasi. Kesimpulan: Dapat disimpulkan bahwa penggunaan mode Q-switched pada laser Nd:YAG sebagai alat dengan kontak minimal dapat dijadikan teknik alternatif untuk meningkatkan kualitas perawatan dental.
Identifikasi epitop dari Streptococcus mutans terhadap sekretori Imunoglobulin A saliva (The identification of Streptococcus mutans epitopes to secretory Immunoglobulin A saliva) Yuliati, Anita
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 3 (2005): (September 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.968 KB) | DOI: 10.20473/j.djmkg.v38.i3.p103-107

Abstract

S. mutans is one of the etiology agent of dental caries, these bacteria have a surface protein of about 185 kDa named Ag I/II. The secretory of sIgA saliva to Ag I/II of S.mutans has shown to be able to prevent colonization in human oral cavity. Peptides derived from the 824 to 853 residues of the P region of antigen I/II S. mutans related to the pathogenesis of dental caries. The aim of this study was to identify the overlapping sequence of amino acids (epitope) derived from the 624 to 853 residues of P of antigen I/II S. mutans to sIgA saliva on caries and caries-free subject in a observational cross sectional study. The P region of antigen I/II S.mutans was cut into 22 peptides of 9 mer sequences with an overlapping of 8 mer and an offset of 1 mer, synthesized on polyethylene pins and tested for the reactivity with an ELISA indirect method to sIgA saliva on caries and caries-free subject. The results of this study showed that amino acid sequences with TPPVKP (832–837) and TAPTKPTY (838–845) were reactive to sIgA saliva on caries and caries-free subject. The conclusion of this study was that the overlapping common sequence of amino acid (epitopes) corresponding to TPPVKP (832–837) and TAPTKPTY (838–845) was identified as caries marker epitopes in human.
Viabilitas sel fibroblas BHK-21 pada permukaan resin akrilik rapid heat cured (Viability of fibroblast BHK-21 cells to the surface of rapid heat cured acrylic resins) Yuliati, Anita
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 2 (2005): (June 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.72 KB) | DOI: 10.20473/j.djmkg.v38.i2.p68-72

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Acrylic resins are widely used in the fabrication of denture bases and have been shown to be cytotoxic as a result of substances that leach from the resin. Numerous reports suggest that residual monomer may be responsible for mucosal irritation and sensitization of tissues. This information is important in eddition to the information of the biologiced effect of such materials. The purpose of this study was to know the viability of fibroblast BHK-21cells to the surface of rapid heat cured acrylic resins. The sample of 5 mm in diameter and 1 mm thickness was cured in water bath for 20, 30, and 40 minutes at 100° C. BHK-21 cells were grown in medium eagle to be 2 × 105 cell/ml in 96 well micro titer plates as the added sample and incubated at 37° C for 24 hour. Five hours before the end of the incubation MTT solutionwas added from step one to each well containing cells. Viability cells were measured by spectrophotometer at 550 nm. The data were statistically analyzed by using one-way analysis of variance followed by LSD test. The result indicated that viability of fibroblast BHK-21 cells did not decrease to the surface of resin acrylic rapid heat cured.
Perubahan warna semen ionomer kaca setelah direndam dalam larutan teh hitam Wardhani, Wahyu Puri; Meizarini, Asti; Yuliati, Anita; Apsari, Retna
Journal of Dentomaxillofacial Science Vol 9, No 2 (2010): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v9i2.242

Abstract

Microporosity in glass ionomer cement contributes on its colour stability. In oralcavity GIC interacts with saliva, foods, drinks, and the oral hygiene condition whichcould affect in colour stability. This study aimed to examine the colour change in GICafter immersion in black tea solution. Twenty eight disc specimens (6 mm diameterand 1 mm thickness). The colour measurements were recorded after storage in closedtube for 24 hours. Specimens were randomly devided into 4 groups of 7 specimens.Control specimens were kept in saline solution. The other 3 groups were immersed inblack tea solution for 5 minutes respectively for 5, 7, and 14 times, followed by colourchange measurement. Data were submitted to one-way ANOVA and LSD. There wassignificant colour change in GIC after immersion in black tea. The conclusion islonger GIC immersed in black tea lower the intensity. The lowest intensity changingwas the immersed for 5 minutes, 14 times daily.
Compressive strength and porosity tests on bovine hydroxyapatite-gelatin-chitosan scaffolds Kartikasari, Nadia; Yuliati, Anita; Kriswandini, Indah Liatiana
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 3 (2016): (September 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.975 KB) | DOI: 10.20473/j.djmkg.v49.i3.p153-157

Abstract

Background: Degenerative diseases, aggressive periodontitis, trauma, jaw resection, and congenital abnormalities can cause defects in jaw bone. The surgical procedure for bone reconstruction currently performed is bone regeneration graft (BRG). Unfortunately, this procedure still has many disadvantages. Thus, tissue engineering approach is necessary to be conducted. The main component used in this tissue engineering is scaffolds. Scaffolds used in bone regeneration is expected to have appropriate characteristics with bone, such as high porosity and swelling ratio, low degradation rates, and good mechanical properties. For those reasons, this research used scaffolds made from bovine hydroxyapatite (BHA), gelatin (GEL), and chitosan (K)/BHA-GEL-K as one of biomaterial candidates for bone regeneration. Purpose: This study aimed to determine compressive strength value and porosity size of BHA-GEL-K scaffolds. Method: Compressive strength of BHA-GEL-K scaffolds was tested using autograph with speed 10 mm/ min with a load cell compress machine of 100 kN. Compressive strength was calculated by force divided to surface area. Porosity test was measured using SEM. Scaffold were coated with Pb and Au, then the porosity size is calculated with SEM at 100x magnification. Result: BHA-GEL-K scaffolds had a mean compressive strength value of 174.29 kPa and a porosity size of 31.62 + 147.06 lm. Conclusion: It can be concluded that BHA-GEL-K scaffolds has a good compressive strength, but not yet resemble real bone mass, while porosity of BHA-GEL-K scaffold is appropriate for bone tissue regeneration application.
Toxicity testing of chitosan from tiger prawn shell waste on cell culture Ariani, Maretaningtias Dwi; Yuliati, Anita; Adiarto, Tokok
Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 1 (2009): (March 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.503 KB) | DOI: 10.20473/j.djmkg.v42.i1.p15-20

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Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, and allergenic. Chitosan represents a new biomaterial in dentistry. Purpose: To examine the toxicity of chitosan from tiger prawn shell waste on cell culture with MTT assay. Methods: Chitosan with concentration of 0.25%, 0.5%, 0.75% and 1% was used in this experiment. Each sample was immersed on eppendorf microtubes containing media culture. After 24 hours, the immersion of media culture was used to examine the toxicity effects on BHK-21 cell based on MTT assay method. The density of optic formazan indicates the number of living cells. All data were then statistically analyzed by one-way Anava. Results: The number of living cells in chitosan from tiger prawn shell waste was 93.16%; 85.07%; 78.48%; 75.66%. Thus, there was no significant difference among groups. Conclusion: Chitosan with 0.25%, 0.5%, 0.75% and 1% concentrations from tiger prawn shell waste were not toxic for BHK-21 cell culture when using parameter CD50.
Perlekatan koloni Streptococcus mutans pada permukaan resin komposit sinar tampak (The adherence of Streptococcus mutans colony to surface visible light composite resins) Anggraeni, Ajeng; Yuliati, Anita; Nirwana, Intan
Dental Journal (Majalah Kedokteran Gigi) Vol 38, No 1 (2005): (March 2005)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.236 KB) | DOI: 10.20473/j.djmkg.v38.i1.p8-11

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Visible light composite resins was used to restore anterior and posterior teeth, and it is always covered by saliva pellicle. S. mutans can adhere to all of the surface of oral cavity and visible light composite resins. The aim of this study was to know the amount of S. mutans colony adherence to visible light composite resins surface. The sample of 5 mm diameter and 3 mm in thickness was immersed in saliva for one hour, than the samples were put into bacteria suspension, incubated for 24 hours at 37° C. The amount of S. mutans was determined by direct count using microscope. The data were statistically analyzed by using t test. The result showed a significance difference of S. mutans colony between hybrid and micro fill visible light composite resins. The conclusion was that the amount of S. mutans adherence on the surface of hybrid was higher than the micro fill visible light composite resins.
Surface hardness of hybrid ionomer cement after immersion in antiseptic solution Yuliati, Anita; Wardani, Ajeng Kartika Sri
Dental Journal (Majalah Kedokteran Gigi) Vol 39, No 2 (2006): (June 2006)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.308 KB) | DOI: 10.20473/j.djmkg.v39.i2.p85-88

Abstract

Hybrid ionomer cement or resin modified glass ionomer cement is a developed form of conventional glass ionomer cement. This hybrid ionomer cement can be eroded if in direct contact with acid solution which will affect surface hardness. The aim of this study is to learn surface hardness of hybrid ionomer cement after immersion in methyl salicylate 0.06% (pH 3.6) and povidon iodine 1% (pH 2.9) solution. Sample of hybrid ionomer cement with 5 mm diameter and 3 mm thickness was immersed in sterile aquadest solution (control), methyl salicylate pH 3.6, povidon iodine pH 2.9 for 1 minute, 7 and 14 minutes. Surface hardness was measured with Micro Vickers Hardness Tester. The obtained data was analyzed statistically with ANOVA followed by LSD test. The result of hybrid ionomer cement after immersion in sterile aquadest, methyl salicylate 0.06% pH 3.6 and povidon iodine 1% pH 2.9 for one minute, showed no significant difference; while immersion for 7 and 14 minutes showed a significant difference. The conclusion states that hybrid ionomer cement after 14 minutes immersion in povidon iodine 1% pH 2.9 has the lowest surface hardness.