- Sudigdoadi, -
Departemen Ilmu Kesehatan Kulit dan Kelamin Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung

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Kadar Interleukin-18 pada Kultur Limfosit Penderita Dermatitis Atopik yang Distimulasi Staphylococcal Enterotoxin B (SEB) Suwarsa, Oki; Sudigdoadi, -; Sutedja, Endang; Idjradinata, Ponpon
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Staphylococcus aureus (S. aureus) mempunyai peranan penting pada patogenesis dermatitis atopik (DA). Peran S. aureus tersebut tidak hanya sebagai pencetus DA, tetapi juga menyebabkan inflamasi kronik. Peran tersebut berhubungan dengan dihasilkannya protein antara lain toksin poten oleh S. aureus, yaitu Staphylococcal enterotoxin B (SEB). Interleukin-18 (IL-18) merupakan regulator penting dari produksi sitokin Th-1 yaitu interferon-γ (IFN-γ).  Tujuan penelitian ini adalah mengetahui kadar IL-18 pada kultur limfosit pasien DA yang distimulus dengan SEB. Penelitian ini dilakukan pada 20 orang penderita DA (7 laki-laki dan 13 perempuan) dan 20 orang sehat (9 laki-laki dan 11 perempuan) di Rumah Sakit Dr. Hasan Sadikin Bandung, merupakan penelitian eksperimental secara in vitro pada kultur ilmfosit yang distimulus dengan SEB di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada. Terjadi peningkatan kadar IL-18 rata-rata pada kultur limfosit antara sebelum dipapar dan setelah dipapar SEB, baik pada kelompok DA maupun kelompok kontrol. Setelah dilakukan uji statistik perbandingan antara kadar IL-18 rata-rata sebelum dan sesudah dipapar SEB antara kelompok DA dan kontrol, didapatkan hasil kadar IL-18 kelompok DA lebih tinggi bermakna dibanding  dengan kelompok kontrol (p<0,05) sehingga dapat disimpulkan kadar IL-18 meningkat tinggi pada kelompok DA yang dipapar SEB. [MKB. 2015;47(4):249–54]Kata kunci: Dermatitis atopik, interleukin-18 (IL-18), Staphylococcus enterotoxin BInterleukin-18 Levels in Lymphocytes Cultures from Atopic Dermatitis Patients Stimulated by Staphylococcal Enterotoxin BAbstractStaphylococcus aureus (S. aureus) has an important role in the pathogenesis of atopic dermatitis (AD). S. aureus acts as a triggering factor for AD and also causes chronic inflammation. These roles of S. aureus are related to various proteins such as Staphylococcal enterotoxin B (SEB) as a potent toxin. Interleukin-18 (IL-18) is an important regulator of cytokine production of Th-1, which is interferon-γ (IFN-γ). The aim of this study was to reveal the levels of IL-18 in cultured lymphocytes from AD patients exposed by SEB. This study was conducted on 20 people with DA (7 men and 13 women) and 20 healthy volunteers (9 men and 11 women) in dr. Hasan Sadikin General Hospital. The in vitro experimental study on cultured lympocytes exposed with SEB was performed at the Integrated Research and Testing Laboratory of Gadjah Mada University. The average levels of IL-18 in cultured lymphocytes before and after being exposed to SEB increased both in AD group and control group. After the statistical tests was performed on the ratio of the average levels of IL-18 before and after being exposed to SEB between AD and control groups, it was shown that the levels of IL-18 AD group was significantly higher than the control group (p <0.05). Therefore, it can be concluded that the levels of IL-18 increased higher in AD group exposed by SEB. [MKB. 2015;47(4):249–54]Key words: Atopic dermatitis , interleukin-18, Staphylococcal enterotoxin BDOI: 10.15395/mkb.v47n4.620
Potensi Suplementasi Resveratrol dalam Mengatasi Efek Negatif Hormon Tiroid pada Terapi Obesitas: Uji Preklinik Ruslami, Rovina; Rosalita, Renny; Yolanda, Raesa; Agustin, Agnes; Sudigdoadi, -
Majalah Kedokteran Bandung Vol 48, No 1 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Terapi obesitas menggunakan hormon tiroid menurunkan berat badan dengan nyata, namun menyebabkan berbagai efek negatif seperti stres oksidatif, kerusakan DNA, dan muscle wasting. Penelitian ini bertujuan mengkesplorasi potensi resveratrol (RSV), polifenol alami yang mempunyai efek antioksidan, anti-inflamasi, dan antiobesitas. Uji preklinik dengan rancangan acak sederhana dilakukan di Lab Hewan Fakultas Kedokteran Universitas Padjadjaran menggunakan 40 ekor tikus Wistar yang diinduksi obesitas. Hewan model obesitas dialokasikan secara acak ke dalam dua kelompok selama periode Agustus–November 2014. Kelompok perlakuan mendapat hormon tiroid 3 (HT3) dan RSV, serta kelompok kontrol mendapat HT3 saja selama 14 hari. Hewan coba yang mendapat HT3 dan RSV memiliki bobot badan lebih besar (290,5 g vs 251 g, p=0,016), massa otot lebih berat (2,57 g vs 2,25 g, p=0,036), indeks adiposit lebih kecil (0,89% vs 1,02%, p=0,026), dan area under the curve (AUC) gukosa lebih kecil (1.849,2 mg.mnt/dL vs 2.591,7 mg.mnt/dL, p<0,001) dibanding yang mendapat HT3 saja. Tidak terdapat perbedaan kadar MDA plasma (0,49 nmol/mL vs 0,44 nmol/mL, p=0,430) dan kerusakan DNA (19,7 pixel vs 22,0 pixel, p=0,657) pada kedua kelompok yang mungkin berhubungan dengan dosis dan lama pemberian resveratrol. Suplementasi resveratrol berpotensi mengatasi efek negatif hormon tiroid pada hewan model obesitas. Perlu penelitian lanjutan untuk mengetahui dosis optimal resveratrol sebagai antioksidan pada pengobatan obesitas menggunakan hormon tiroid. [MKB. 2016;48(1):51–7]Kata kunci: Hormon tiroid, obesitas, resveratrol, uji preklinikResveratrol Supplementation Potential in Managing Negative Effects of Thyroid Hormone during Obesity Treatment: a Pre-Clinical TrialAbstractTreatment of obesity using thyroid hormone could give significant result in decreasing body weight, but it also causes various negative effects such as oxidative stress, DNA damage, and muscle wasting. This sudy aimed to explore the potential of resveratrol, a natural polyphenol, that has anti-oxidant, anti-inflammatory, and anti-obesity effects, in managing negative effects of thyroid hormone used as anti-obesity. A preclinical trial was conducted at the animal laboratory, Faculty of Medicine, Universitas Padjadjaran. Forty male Wistar rats underwent obesity induction before randomly allocated into intervention and control groups in August–November 2014. The intervention group received a 14-days of triiodothyronine (T3) and resveratrol (RSV), while the control group received T3 only. Obese rats treated with T3 and RSV were heavier (body weight of 290.5 g vs 251.0 g, p<0.016), had a heavier muscle mass (2.57 g vs 2.25 g, p=0.036), smaller adipocyte index, (0.89% vs 1.02%, p=0.026) and smaller glucose area under the curve (AUC) (1,848.2 mg.mnt/dL vs 2,591.7 mg.mnt/dL, p<0.001) than those who were treated with T3 only. There was no significance difference in MDA plasma levels (0.49 nmol/mL vs 0.44 nmol/mL, p=0.0430) and DNA damage (19.7 pixel vs 22.0 pixel, p=0.657) between groups, probably due to insufficient dosage of resveratrol. Our results indicate that resveratrol supplementation could counter negative effects of thyroid hormone used as anti-obesity in obese-rats model treated with T3. More research is needed to determine the adequate dosage of resveratrol to elicit anti-oxidant effect that could minimize the negative effects of thyroid hormone as anti-obesity. [MKB. 2016;48(1):51–7]Key words: Obesity, preclinical trial, resveratrol, thyroid hormone DOI: 10.15395/mkb.v48n1.734
Kadar Interleukin-18 pada Kultur Limfosit Penderita Dermatitis Atopik yang Distimulasi Staphylococcal Enterotoxin B (SEB) Suwarsa, Oki; Sudigdoadi, -; Sutedja, Endang; Idjradinata, Ponpon
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Staphylococcus aureus (S. aureus) mempunyai peranan penting pada patogenesis dermatitis atopik (DA). Peran S. aureus tersebut tidak hanya sebagai pencetus DA, tetapi juga menyebabkan inflamasi kronik. Peran tersebut berhubungan dengan dihasilkannya protein antara lain toksin poten oleh S. aureus, yaitu Staphylococcal enterotoxin B (SEB). Interleukin-18 (IL-18) merupakan regulator penting dari produksi sitokin Th-1 yaitu interferon-γ (IFN-γ).  Tujuan penelitian ini adalah mengetahui kadar IL-18 pada kultur limfosit pasien DA yang distimulus dengan SEB. Penelitian ini dilakukan pada 20 orang penderita DA (7 laki-laki dan 13 perempuan) dan 20 orang sehat (9 laki-laki dan 11 perempuan) di Rumah Sakit Dr. Hasan Sadikin Bandung, merupakan penelitian eksperimental secara in vitro pada kultur ilmfosit yang distimulus dengan SEB di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada. Terjadi peningkatan kadar IL-18 rata-rata pada kultur limfosit antara sebelum dipapar dan setelah dipapar SEB, baik pada kelompok DA maupun kelompok kontrol. Setelah dilakukan uji statistik perbandingan antara kadar IL-18 rata-rata sebelum dan sesudah dipapar SEB antara kelompok DA dan kontrol, didapatkan hasil kadar IL-18 kelompok DA lebih tinggi bermakna dibanding  dengan kelompok kontrol (p<0,05) sehingga dapat disimpulkan kadar IL-18 meningkat tinggi pada kelompok DA yang dipapar SEB. [MKB. 2015;47(4):249–54]Kata kunci: Dermatitis atopik, interleukin-18 (IL-18), Staphylococcus enterotoxin BInterleukin-18 Levels in Lymphocytes Cultures from Atopic Dermatitis Patients Stimulated by Staphylococcal Enterotoxin BAbstractStaphylococcus aureus (S. aureus) has an important role in the pathogenesis of atopic dermatitis (AD). S. aureus acts as a triggering factor for AD and also causes chronic inflammation. These roles of S. aureus are related to various proteins such as Staphylococcal enterotoxin B (SEB) as a potent toxin. Interleukin-18 (IL-18) is an important regulator of cytokine production of Th-1, which is interferon-γ (IFN-γ). The aim of this study was to reveal the levels of IL-18 in cultured lymphocytes from AD patients exposed by SEB. This study was conducted on 20 people with DA (7 men and 13 women) and 20 healthy volunteers (9 men and 11 women) in dr. Hasan Sadikin General Hospital. The in vitro experimental study on cultured lympocytes exposed with SEB was performed at the Integrated Research and Testing Laboratory of Gadjah Mada University. The average levels of IL-18 in cultured lymphocytes before and after being exposed to SEB increased both in AD group and control group. After the statistical tests was performed on the ratio of the average levels of IL-18 before and after being exposed to SEB between AD and control groups, it was shown that the levels of IL-18 AD group was significantly higher than the control group (p <0.05). Therefore, it can be concluded that the levels of IL-18 increased higher in AD group exposed by SEB. [MKB. 2015;47(4):249–54]Key words: Atopic dermatitis , interleukin-18, Staphylococcal enterotoxin BDOI: 10.15395/mkb.v47n4.620
Potensi Suplementasi Resveratrol dalam Mengatasi Efek Negatif Hormon Tiroid pada Terapi Obesitas: Uji Preklinik Ruslami, Rovina; Rosalita, Renny; Yolanda, Raesa; Agustin, Agnes; Sudigdoadi, -
Majalah Kedokteran Bandung Vol 48, No 1 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Terapi obesitas menggunakan hormon tiroid menurunkan berat badan dengan nyata, namun menyebabkan berbagai efek negatif seperti stres oksidatif, kerusakan DNA, dan muscle wasting. Penelitian ini bertujuan mengkesplorasi potensi resveratrol (RSV), polifenol alami yang mempunyai efek antioksidan, anti-inflamasi, dan antiobesitas. Uji preklinik dengan rancangan acak sederhana dilakukan di Lab Hewan Fakultas Kedokteran Universitas Padjadjaran menggunakan 40 ekor tikus Wistar yang diinduksi obesitas. Hewan model obesitas dialokasikan secara acak ke dalam dua kelompok selama periode Agustus–November 2014. Kelompok perlakuan mendapat hormon tiroid 3 (HT3) dan RSV, serta kelompok kontrol mendapat HT3 saja selama 14 hari. Hewan coba yang mendapat HT3 dan RSV memiliki bobot badan lebih besar (290,5 g vs 251 g, p=0,016), massa otot lebih berat (2,57 g vs 2,25 g, p=0,036), indeks adiposit lebih kecil (0,89% vs 1,02%, p=0,026), dan area under the curve (AUC) gukosa lebih kecil (1.849,2 mg.mnt/dL vs 2.591,7 mg.mnt/dL, p<0,001) dibanding yang mendapat HT3 saja. Tidak terdapat perbedaan kadar MDA plasma (0,49 nmol/mL vs 0,44 nmol/mL, p=0,430) dan kerusakan DNA (19,7 pixel vs 22,0 pixel, p=0,657) pada kedua kelompok yang mungkin berhubungan dengan dosis dan lama pemberian resveratrol. Suplementasi resveratrol berpotensi mengatasi efek negatif hormon tiroid pada hewan model obesitas. Perlu penelitian lanjutan untuk mengetahui dosis optimal resveratrol sebagai antioksidan pada pengobatan obesitas menggunakan hormon tiroid. [MKB. 2016;48(1):51–7]Kata kunci: Hormon tiroid, obesitas, resveratrol, uji preklinikResveratrol Supplementation Potential in Managing Negative Effects of Thyroid Hormone during Obesity Treatment: a Pre-Clinical TrialAbstractTreatment of obesity using thyroid hormone could give significant result in decreasing body weight, but it also causes various negative effects such as oxidative stress, DNA damage, and muscle wasting. This sudy aimed to explore the potential of resveratrol, a natural polyphenol, that has anti-oxidant, anti-inflammatory, and anti-obesity effects, in managing negative effects of thyroid hormone used as anti-obesity. A preclinical trial was conducted at the animal laboratory, Faculty of Medicine, Universitas Padjadjaran. Forty male Wistar rats underwent obesity induction before randomly allocated into intervention and control groups in August–November 2014. The intervention group received a 14-days of triiodothyronine (T3) and resveratrol (RSV), while the control group received T3 only. Obese rats treated with T3 and RSV were heavier (body weight of 290.5 g vs 251.0 g, p<0.016), had a heavier muscle mass (2.57 g vs 2.25 g, p=0.036), smaller adipocyte index, (0.89% vs 1.02%, p=0.026) and smaller glucose area under the curve (AUC) (1,848.2 mg.mnt/dL vs 2,591.7 mg.mnt/dL, p<0.001) than those who were treated with T3 only. There was no significance difference in MDA plasma levels (0.49 nmol/mL vs 0.44 nmol/mL, p=0.0430) and DNA damage (19.7 pixel vs 22.0 pixel, p=0.657) between groups, probably due to insufficient dosage of resveratrol. Our results indicate that resveratrol supplementation could counter negative effects of thyroid hormone used as anti-obesity in obese-rats model treated with T3. More research is needed to determine the adequate dosage of resveratrol to elicit anti-oxidant effect that could minimize the negative effects of thyroid hormone as anti-obesity. [MKB. 2016;48(1):51–7]Key words: Obesity, preclinical trial, resveratrol, thyroid hormone DOI: 10.15395/mkb.v48n1.734