Uslan Uslan, Uslan
Program Studi Magister Biologi, Program Pascasarjana Universitas Udayana, Jalan PB Sudirman, Denpasar, Bali

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Optimasi Konsentrasi DNA dan MgCl2 pada Reaksi Polymerase Chain Reaction-Random Amplified Polymorphic DNA untuk Analisis Keragaman Genetik Tanaman Faloak (Sterculia quadrifida R.Br) (Optimization of DNA and MgCl2 Concentrations in Polymerase Chain Reacti Uslan, Uslan; Pharmawati, Made
JURNAL BIOS LOGOS Vol 5, No 1 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.1.2015.9316

Abstract

Abstrak Faloak  merupakan tanaman yang tumbuh di lahan kritis. Sebagai upaya mendukung pemuliaan dan konservasi tanaman faloak diperlukan informasi keragaman genetiknya. Salah satu metode analisis keragaman genetik adalah menggunakan penanda DNA yang berbasis PCR. Untuk itu diperlukan kondisi PCR (Polymerase Chain Reaction) yang tepat sehingga diperoleh hasil yang dapat dianalisis lebih lanjut. Penelitian ini bertujuan menentukan kondisi optimum PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) tanaman faloak. Ekstraksi DNA dilakukan dengan metode CTAB. Optimasi dilakukan dengan menggunakan beberapa konsentrasi DNA cetakan dan MgCl2. Kondisi optimum PCR-RAPD tanaman faloak yang menghasilkan pita produk PCR yang jelas diperoleh  menggunakan 50 ng/ul DNA, 3 mM MgCl2 serta jumlah siklus termal 45 x. Kata kunci : PCR-RAPD, optimasi, tanaman faloak Abstract Faloak is a plant that grows on critical lands. In an effort to support breeding and conservation of faloak, information about its genetic diversity is required. One of the methods of genetic diversity analysis is using PCR-based DNA markers. For that purpose, proper PCR conditions is needed in order to obtain results that can be further analyzed. This study aimed to determine the optimum conditions for PCR-RAPD of faloak plants. DNA extraction was conducted using CTAB. Optimization was done by using several concentrations of DNA templates and MgCl2. The optimum conditions of PCR-RAPD of faloak plants that produce clear band of PCR products were obtained using 50 ng/ ul DNA, 3 mM MgCl2 and 45x thermal cycles Keywords : PCR-RAPD, optimization, faloak plant
MODEL STUDENT CENTERED LEARNING (SCL) DI SEKOLAH DASAR (SD) YANG INOVATIF TERINTEGRASI PEMBELAJARAN TERPADU Uslan, Uslan; Nuriyah, Nuriyah
PENDAS MAHAKAM: Jurnal Pendidikan Dasar Vol 3, No 1 (2018)
Publisher : PENDAS MAHAKAM: Jurnal Pendidikan Dasar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Learning model as a conceptual framework used as a guide in learning. An example of a commonly used learning model is student centered learning (SCL). Thus, the adoption of an innovative SCL model helps learners understand concepts by connecting between the content learned and the real life of learners that are integrated into The objective of this research is to integrate integrated learning for students in elementary school (SD) into innovative SCL model.This type of research is literature research with documentation study method from various sources of books and scientific journals Discussion of research, reviewing: innovative integrated learning models in SD, SCL model function in KBM, integrated learning benefits, and SCL model correlation with integrated learning.Keywords: SCL SD Model, Innovative, Integrated Learning