Beni Lestari, Beni
Farmasi, Fakultas Farmasi, Universitas Gadjah Mada

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POTENSI BIJI LABU KUNING SEBAGAI AGEN FITOESTROGEN PADA WANITA POST MENSTRUAL

Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

Osteoporosis and hypercholesterolemia are prevalent condition in menopausal women. The common therapy to prevent the estrogen degrading condition is Hormone Replacement Therapy (HRT). However, HRT possessed various risks. Curcubita pepo L. seed (pumpkin seed) contains lignan secoisolariciresinol and lariciresinol which exhibit estrogenic effect. The aim of this study is to determine the estrogenic effect of Ethanolic Extract of Pumpkin Seeds (EEPS) through in silico and in vivo study. In silico study were conducted by molecular docking of lignan which is secoisolariciresinol and lariciresinol with Estrogen Receptor (ERα and Erβ). In vivo study conducted by using ovariectomized Sprague dawley female rats as a model of postmenopausal women. Blood lipid profile, bone density, and uterine weight were assayed after thirty days. Molecular docking score of secoisolariciresinol and lariciresinol were similar to estradiol. In vivo study found that EEPS increase bone density and uterine weight percentage while also improve the blood profile. In conclusion, these result showed that EEPS is potential to be developed as an osteoporosis and hypercholesterolemia prevention agent. Keywords:  Lime peel, Limonene, Aromatic candles, Repellent

PENERAPAN MODEL TARI BAMBU (BAMBOO DANCING) DENGAN MEDIA KARTU DALAM PENINGKATAN HASIL BELAJAR PENDIDIKAN KEWARGANEGARAAN TENTANG ORGANISASI DI KELAS V SEKOLAH DASAR

KALAM CENDEKIA PGSD KEBUMEN Vol 5, No 5.1 (2017): KALAM CENDEKIA PGSD KEBUMEN (EDISI KHUSUS)
Publisher : KALAM CENDEKIA PGSD KEBUMEN

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Abstract

Abstrak: Penerapan Model Tari Bambu (Bamboo Dancing) dengan Media Kartu dalam Peningkatan Hasil Belajar PKn tentang Organisasi siswa kelas V SDN 2 Ngasinan. Penelitian ini bertujuan untuk mendeskripsikan langkah-langkah penerapan model pembelajaran model tari bambu dengan media kartu,  meningkatkan hasil belajar PKn tentang organisasi di kelas V SDN 2 Ngasinan tahun ajaran 2016/2017, dan mendeskripsikan  kendala dan  solusinya dalam peningkatan hasil belajar PKn di kelas V SDN 2 Ngasinan tahun ajaran 2016/2017. Penelitian ini merupakan Penelitian Tindakan Kelas (PTK) kolaboratif yang dilaksanakan dalam tiga siklus. Tiap siklus terdiri atas empat unsur yaitu perencanaan, pelaksanaan, pengamatan, dan refleksi. Subjek penelitian ini adalah siswa kelas V SDN 2 Ngasinan yang berjumlah 31 siswa yang terdiri dari 13 laki-laki dan 18 perempuan. Objek penelitian ini adalah peningkatan hasil belajar siswa. Hasil penelitian ini menunjukkan bahwa penerapan model tari bambu dengan media kartu dapat meningkatkan hasil belajar PKn siswa kelas V SDN 2 Ngasinan.Kata Kunci: Model Tari Bambu, Media Kartu, Hasil Belajar PKn.

Reveal Cytotoxicity and Antigenotoxicity of Piper nigrum L. Ethanolic Extract and its Combination with Doxorubicin on CHO-K1 Cells

Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

Black pepper (Piper nigrum L.), one of the most popular Indonesian spices has been reported to possess various therapeutic effects. The aim of this study is to evaluate the cytotoxicity and antigenotoxicity of black pepper ethanolic extract (BPE) and its combination with doxorubicin (Dox) on CHO-K1 cells. Based on thin layer chromatographyanalysis, BPE contained piperine.Under MTT assay, BPE showed cytotoxic effect with the IC50 value of 68 μg/mL and performed synergism in combination with Dox. In vitro micronucleus test using Giemsa staining revealed that BPE did not cause morphological changes qualitatively on CHO-K1 cells at concentration of 8.5 μg/mL, whereas using flow cytometry analysis showed that BPE could decrease the number of micronucleus (MN) formation induced by doxorubicin. In addition, BPE reduced the ROS level on the CHO-K1 cells which observed by reactive oxygen species (ROS) intracellular assay. The decrease in ROS level indicated that the antioxidant activity of BPE contribute to the antigenotoxicity. Furthermore, molecular docking performed that piperine interacted with DNA Topoisomerase II with docking score of -80.68. Overall,BPE performed cytotoxic effect in single treatment, increased the cytotoxicity and reduced the genotoxicity of doxorubicin. Thus, BPE has potential to be developed further as co-chemotherapeutic and antigenotoxic agent.Keywords: Cytotoxic, genotoxic, Piper nigrum L., CHO-K1, micronucleus

Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words : food additives, genotoxic, cytotoxic, micronucleus

A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds

Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

The pumpkin belongs to the family of Cucurbitaceae, is a well-known edible plant that has been frequently used as functional food or herbal medicine. Pumpkins contain rich unsaturated fatty acids, phytoestrogens and vitamins E in their seeds that have potential pharmaceutical, nutraceutical, and cosmeceutical properties. Information regarding their nutritional components and therapeutic properties of pumpkin seeds has expanded dynamically in the recent years and this review focus on the three main components of pumpkin seeds that described before. Several types of unsaturated fatty acids are the dominant component in pumpkin seeds which can play a role in the disease prevention and promote health. Pumpkin seeds also contain the important phytoestrogen compounds, i.e., secoisolariciresinol and lariciresinol that have estrogenic-like effect such as preventing hyperlipidemia and osteoporosis for menopausal women. Phytoestrogens in pumpkin seeds also could be related to a reduced hormone-dependent tumor. Pumpkin seeds are rich in vitamin E contents as an emerging free radical scavenger, anti-aging and antioxidant such as a-tocopherol and g-tocopherol. Findings of these studies prove that patents field for the innovation product of pumpkin seeds holds promise for the future along with their immense nutraceutical properties.Keywords : pumpkin seeds, estrogenic, anticancer, antioxidant, nutraceutical

A Comparison of Antimetastatic Activity between Nerium indicum and Cinnamomum burmannii on 4T1 Cells

Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

Metastatic process becomes a major problem in advanced cancer cases. Natural compounds found in several plants in Indonesia have a potency to be developed as chemoterapeutic agent which are targeted to metastatic process. Jure leaves (Nerium indicum) which contain oleandrin and cinnamaldehyde in cinnamon bark (Cinnamomum burmannii) reported to have cytotoxic activity on several cancer cells, but their activities on metastasic process have never been explored. This research aims to reveal and to compare their anti-metastatic effect toward 4T1 breast cancer cells. The cytotoxicity of jure leaves extract (JLE) and cinnamon essential oil (CEO) was obtained by MTT assay. Metastatic process mainly on cell migration was examined by scratch wound healing assay while MMP-9 expression that described the invassion process was observed by gelatin zymography assay. Molecular interaction between their active compounds and MMP-9 receptor was predicted by molecular docking. The result showed that treatment with JLE and CEO inhibited the growth of 4T1 cells with IC50 value of 125 µg/mL and 2.5 µg/mL, respectively. In addition, JLE performed inhibitory effect of cell migration better than CEO. Meanwhile, both JLE and CEO decreased MMP-9 protein expression. Thus, JLE and CEO have potentials to be developed as an anti-metastatic agent and JLE could be more effective.Key words: Nerium indicum, Cinnamomum burmannii, anti-metastasis, scratch assay, gelatin zimography