Mulyadi Adam, Mulyadi
Unknown Affiliation

Published : 27 Documents
Articles

Found 27 Documents
Search

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (223.054 KB)

Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (>2 years), six kids (<6 months), and six does (>2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat

STEROID LEVEL AND PREGNANCY RATE OF ACEH COWS IN RESPONSE TO OVULATION INDUCTION USING PRESYNCHOVSYNCH METHOD

Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (424.63 KB)

Abstract

Penelitian ini bertujuan mengetahui peningkatan level steroid dan persentase kebuntingan sapi aceh terhadap induksi ovulasi dengan metode presynch-ovsynch. Dalam penelitian ini digunakan sepuluh ekor sapi aceh betina dengan status tidak bunting, minimal dua bulan pascapartus, sudah pernah beranak, dan sehat secara klinis. Sapi dibagi atas dua kelompok, yang masing-masing terdiri atas lima ekor sapi. Kelompok pertama (K1) disinkronisasi berahi dengan metode presynch-ovsynch. Pada kelompok kedua (K2), disinkronisasi berahi menggunakan 5 ml PGF2α secara intramuskulus dengan pola penyuntikan ganda dengan interval 12 hari. Setelah  48 jam akhir perlakuan, sapi pada K1 dan K2 diinseminasi menggunakan semen beku fertil. Observasi berahi dilakukan setelah penyuntikan terakhir. Koleksi darah untuk pemeriksaan level estradiol dilakukan segera setelah inseminasi dilakukan sedangkan koleksi darah untuk pemeriksaan progesteron dilakukan pada hari ke-7 pasca-inseminasi. Level steroid diukur menggunakan teknik enzyme-linked immunosorbent assay (ELISA). Pemeriksaan kebuntingan dilakukan 90 hari pasca-inseminasi menggunakan teknik palpasi rektal. Seluruh sapi menunjukkan gejala berahi setelah perlakuan. Level estradiol dan progesteron pada K1 vs K2 masing-masing adalah 294,98±110,48 vs 392,76±11,6 pg/ml (P>0,05) dan 23,85±15,14vs 12,69±5,64ng/ml (P>0,05). Persentase kebuntingan pada K1 vs K2 masing-masing adalah 60,0 vs 0,0%. Dari hasil penelitian disimpulkan bahwa metode presynch-ovsynch tidak dapat meningkatkan level steroid tetapi dapat meningkatkan persentase kebuntingan pada sapi aceh.

The Effect of Bull Age and Ejaculation Frequency on Quality of Aceh Bull Spermatozoa

Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (141.548 KB)

Abstract

The purpose of this research was find out the effect of age and ejaculation frequency on quality of Aceh bull spermatozoa.. This research was conducted at Frozen Semen Prossesing Laboratorium, Saree, Aceh Besar from Januari to April 2013. Six healthy aceh bulls were divided into two groups of age, those were 3.0-4.0 years old; and 4.0-5.0 years old. Three numbers of bull at each group were assumed as the replication. Semen was collected once a week using artificial vagina and collected until third semen ejaculation. After collecting, volume, pH, color, consistency, concentration, motility, mortality, and abnormality of spermatozoa were immediately measured. This data was analyzed by analysis of variance (ANOVA) two-way pattern, followed by Duncant test. The result showed that the evaluation of bull semen at 3.0-4.0 years old and 4.0-5.0 years old affect significantly (P<0.05) on semen volume and spermatozoa concentration and had no contribute significantly on motility, mortality and abnormality of spermatozoa,while ejaculation frequency effect significantly (P<0.05) on semen volume, concentration, and abnormality spermatozoa, but had no effect significantly on motility and mortality (P>0.05). It is concluded that aged and ejaculation frequency effect significantly on quality of aceh bull fresh spermatozoa.Key words: ejaculation frequency, quality of spermatozoa, aceh bull, age

Study of Cat’s Welfare Kept at Pet Shops in Bekasi West Java

Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (218.886 KB)

Abstract

The aim of this study was to find out correlation between cat’s welfare and caring management in a number of cats kept at p etshops inBekasi from January to February 2013. This study used a questionnaire with ratings based on five aspects of animal welfare (feeding, healthy,comfortable, fear, and normal behavior) and through direct observation. Data was analyzed using Correlation Test Chi Square with 95%confidence interval. The result showed that there is the corelation between animal welfare and caring management of cats at pet shop in Bekasi,West Java.____________________________________________________________________________________________________________________Key words: animal welfare, cats, pet shops 

PENGARUH PEMBERIAN GLISEROL DALAM MEDIUM TRIS KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI ACEH SETELAH PEMBEKUAN (The Effect of Giving of Glycerol into Tris-Yolk Medium to the Aceh Cattle Spermatozoa after Freezing)

JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Original Source | Check in Google Scholar | Full PDF (365.079 KB)

Abstract

Spermatoza yang melalui proses pembekuan seringkali mengalami kerusakan karena terbentuknya kristal es. Pembentukan kristal es dapat menyebabkan penurunan kualitas spermatozoa pasca thawing terutama penurunan motilitas. Penambahan krioprotektan dalam pengencer dapat meminimalisir kerusakan sel. Salah satu jenis krioprotektan yang sering digunakan pada pembekuan semen adalah gliserol. Penelitian ini bertujuan untuk melihat pengaruh penambahan gliserol dalam pengencer Tris Kuning Telur (TKT) terhadap kualitas spermatozoa (motilitas, spermatozoa hidup, dan MPU) sapi aceh  yang disimpan dalam nitrogen cair. Desain penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 6 pengulangan. Pengamatan kualitas spermatozoa dilakukan setelah pembekuan di dalam nitrogen cair. Data dianalisis dengan uji Analysis of Variance (ANOVA) pola satu arah. Hasil kualitas spermatozoa pada masing-masing konsentrasi gliserol (3%, 5%, dan 7%) berturut-turut untuk hasil motilitas sebesar: 17,17±12,17%, 51,17±11,13%, dan  33,33±8,76%; hasil spermatozoa hidup sebesar: 22,50±15,41%, 56,33±8,98%, dan 42,50±6,35%; hasil MPU sebesar: 31,42±17,90%, 61,50±10,82%, dan 42,50±12,14%. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan gliserol dalam pengencer Tris Kuning Telur dapat mempertahankan kualitas spermatozoa (motilitas, spermatozoa hidup, dan MPU) sapi aceh setelah pembekuan. Penambahan gliserol dengan konsentrasi 5% dalam pengencer Tris Kuning Telur paling baik dalam mempertahankan kualitas spermatozoa sapi aceh dibandingkan dengan konsentrasi 3% dan 7% (Freezing spermatozoa are often experiencing the damage by the formation of ice crystal. The formation of ice crystal leads to the quality reduction of post-thawing spermatozoa especially reducing motility. The addition of cryoprotectant in the diluent can minimize cell damage. One of the most common cryoprotectant type that was used in sement freezing is glycerol. This research aims to examine the influence of the addition of glycerol in tris-yolk, or known as Tris Egg Yolk (TKT) to the quality of spermatozoa (motility, live spermatozoa, and MPU) of aceh cattles stored in liquid nitrogen. The research design used in this research is a completely randomized design, that consists of three treatments and six repetitions. Quality of spermatozoa observation were performed after freezing process in the liquid nitrogen. Data were analyzed by Analysis of Variance (ANOVA) with one tail pattern. The result of spermatozoa quality in each concentration of glycerol (3%, 5%, and 7%) respectively for the motitity as follows: 17.17±12.17%; 51.17±11.13%; and  33.33±8.76%; result of live spermatozoa as follows: 22.50±15.41%; 56.33±8.98%; and 42.50±6.35%; result of MPU as follows: 31.42±17.90%; 61.50±10.82%; and 42.50±12.14%. Based on the result, the conclusion is that the addition of glycerol in the tris-yolk can maintain the quality of spermatozoa (motility, live spermatozoa, and MPU) of aceh cattle after freezing. The addition of glycerol with 5% concentration in the tris-yolk dilator is the best concentration to maintain the quality of spermatozoa aceh cattle, compared to the 3% and 7% concentration)

pengaruh domestikasi terhadap jenis pakan pada biawak air (varanusa salvator)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Original Source | Check in Google Scholar

Abstract

ABSTRAK Penelitian ini bertujuan mengetahui pengaruh domestikasi terhadap jenis pakan biawak air (Varanus salvator ) dengan pemberian jenis pakan jangkrik dan udang. Dalam penelitian ini digunakan 8 ekor biawak air yang telah didomestikasi (BD), dengan kisaran umur 2-6 bulan dengan berat badan 30-40 g, panjang 20-30 cm dan diberi perlakuan selama 30 hari. Biawak air dibagi ke dalam dua kelompok, kelompok pertama diberikan pakan jangkrik (K I) pada pagi dan sore hari sedangkan kelompok kedua diberikan pakan udang (K II) dengan waktu yang sama. Hasil dari penelitian ini dianalisis dengan menggunakan uji t. Hasil penelitian menunjukkan bahwa jenis pakan biawak air domestikasi yang dikonsumsi pakan jangkrik adalah 16,47±1,77 g dan pakan udang 11,08±1,18 g tidak berbeda nyata (P>0,05). Dapat disimpulkan bahwa domestikasi dari biawak air tidak berpengaruh terhadap jenis pakan.ABSTRACT This study aims to determine the effect of domestication on feeding behavior of water monitor lizard (Varanus salvator) that fed with crickets and shrimps. This study used eight water monitor lizard aged range from 2-6 months, 20-30 cm in long and 30-40 g in weight. The water monitor lizard are divided into two groups, the first groups was given crickets (KI) and the second groups was given shrimps (KII)  in the morning and in the afternoon for 30 days. Data collected was analyzed using t-test. From this studied showed that water monitor lizard consumed crickets and shrimps 16,47±1,77 g and 11,08±1,18 g (P>0,05). The result of the present study show that domestication did not effect types of feed of water monitor lizard

pengaruh domestikasi terhadap jenis pakan pada biawak air (varanusa salvator)

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Original Source | Check in Google Scholar

Abstract

ABSTRAK Penelitian ini bertujuan mengetahui pengaruh domestikasi terhadap jenis pakan biawak air (Varanus salvator ) dengan pemberian jenis pakan jangkrik dan udang. Dalam penelitian ini digunakan 8 ekor biawak air yang telah didomestikasi (BD), dengan kisaran umur 2-6 bulan dengan berat badan 30-40 g, panjang 20-30 cm dan diberi perlakuan selama 30 hari. Biawak air dibagi ke dalam dua kelompok, kelompok pertama diberikan pakan jangkrik (K I) pada pagi dan sore hari sedangkan kelompok kedua diberikan pakan udang (K II) dengan waktu yang sama. Hasil dari penelitian ini dianalisis dengan menggunakan uji t. Hasil penelitian menunjukkan bahwa jenis pakan biawak air domestikasi yang dikonsumsi pakan jangkrik adalah 16,47±1,77 g dan pakan udang 11,08±1,18 g tidak berbeda nyata (P>0,05). Dapat disimpulkan bahwa domestikasi dari biawak air tidak berpengaruh terhadap jenis pakan.ABSTRACT This study aims to determine the effect of domestication on feeding behavior of water monitor lizard (Varanus salvator) that fed with crickets and shrimps. This study used eight water monitor lizard aged range from 2-6 months, 20-30 cm in long and 30-40 g in weight. The water monitor lizard are divided into two groups, the first groups was given crickets (KI) and the second groups was given shrimps (KII)  in the morning and in the afternoon for 30 days. Data collected was analyzed using t-test. From this studied showed that water monitor lizard consumed crickets and shrimps 16,47±1,77 g and 11,08±1,18 g (P>0,05). The result of the present study show that domestication did not effect types of feed of water monitor lizard

DIFERNSIAL LEUKOSIT BIAWAK AIR (Varanus salvator) YANG TELAH DIDOMESTIKASI DAN BIAWAK AIR LIAR

JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Original Source | Check in Google Scholar

Abstract

Penelitianinibertujuanmengetahuidiferensial leukositbiawak air (Varanussalvator) yang telahdidomestikasi dan biawak air liar. Dalampenelitianinidigunakan 16 ekor biawak air yang dibagi dalam dua kelompok, yaitu8 ekorbiawak airyang didomestikasi (BD) dan 8 ekorbiawak air liar hasiltangkapan (BT).KelompokBDdipeliharaselama60 haridandiberimakananikan, udangdanjangkrik.Untukpembuatanpreparatdarahhapus, darahdiambildari BD padaharike 60, sedangkanuntuk BT pengambilan darah dilakukansaatpenangkapan.Hasil penelitian nilaidiferensial leukositBD sebagai berikut:heterofil 36,7%, basofil 13,0%, limfosit 12,0%,eosinofil 15,3%, monosit 14,0%, dan azurofil 8,8%;sedangkan BTsebagai berikut: heterofil29,3%, basofil16,3%,limfosit 14,1%,eosinofil 9,1%,monosit 17,7% danazurofil 13,2%. Hasil analisis datadenganmenggunakanuji Tmenunjukkanbahwadiferensialleukosit BD tidakberbedanyata (P>0.05) dengan BT. Hasil penelitian ini dapat disimpulkan bahwadiferensial leukosit biawak air yang didomestikasi tidak berbeda dengan biawak air liar.

PENGARUH METODE PENCUCIAN SPERMATOZOA SAPI ACEH TERHADAP MOTILITAS, PERSENTASE HIDUP, DAN INTEGRITAS MEMBRAN PLASMA UTUH SPERMATOZOA (The Infuence of Aceh Bull Spermatozoa Washing Method on Spermatozoa Motility and Plasma Membrane Integrity of Intact Spermatozoa)

Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Original Source | Check in Google Scholar | Full PDF (389.844 KB)

Abstract

This study aimed to determine the effect of sperm washing by swim up and centrifugation in isotonic medium on sperm quality of aceh bull. In this study, fresh semen from healthy male aceh bull aged 3-4 months was collected using artificial vagina. Immediately after semen collection, fresh semen quality was examined macroscopically and microscopically. Subsequently, sperm washing was performed by centrifugation and swim up in sperm washing medium. Group 1 (P0) as control group, cement washed with isotonic solution (andromed medium: saline solution) with ratio of 1:8. 2. Group 2 (P1), cement was separated by centrifugation method, group 3 (P2), all cement was separated by swim up method then examined the sperm quality sperm washing results. Each treatment was repeated 5 times. Quality parameters measured were the percentage of spermatozoa motility, sperm viability, and plasma membrane integrity intact spermatozoa. Data were analyzed with analysis of variance one-way pattern, followed by Duncans multiple test. The results showed the mean ± SD percentage of sperm motility of each treatment group (P0; P1; P2) respectively amounted to 72.00±3.74, 66.40±4.77, and 73.60±3.29%. The percentage of viability was 72.00 ±3.74%, 66.40±2.88%, 71.80±2.17%. The percentage of plasma membrane integrity is intact spermatozoa was 68.20±1.79%, 57.20±3.77%, 69.00±2.00%. Results of this study showed that the percentage of motility, live spermatozoa and plasma membrane integrity intact after separation by swim-up method were significantly different (P <0.05) compared with no separation.Key words: spermatozoa quality, aceh bulls, centrifugation, swim up

PERSENTASE SPERMATOZOA HIDUP PADA TIKUS WISTAR DAN SPRAGUE-DAWLEY

Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Original Source | Check in Google Scholar | Full PDF (373.243 KB)

Abstract

Penelitian ini bertujuan menentukan dan membandingkan persentase spermatozoa hidup dari kauda epididimis pada tikus strain Wistar dan Sprague-Dawley. Dua puluh ekor tikus jantan (Rattus norvegicus) yang terdiri dari 10 ekor strain Wistar dan 10 ekor strain Sprague-Dawley berumur 5 bulan digunakan dalam penelitian ini. Pada saat berumur 2,5 bulan seluruh tikus diadaptasikan selama 75 hari di dalam kandang dan diberikan pakan Turbo Feed T.79-4 (PT. Central Protein Prima, Medan) dan minuman secara ad libitum. Setelah masa adaptasi selesai, setiap hari diambil masing-masing dua ekor strain Wistar dan Sprague-Dawley untuk dibedah dan diambil kauda epididimisnya. Selanjutnya dibuat preparat hapus semen dengan menggunakan pewarnaan eosin untuk menghitung jumlah spermatozoa hidup. Preparat diamati di bawah mikroskop dengan 5 lapangan pandang. Data yang diperoleh dianalisis menggunakan uji T. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata persentase spermatozoa yang sangat nyata (P<0,01) antara strain tikus Wistar (92,00±2,83%) dan Sprague-Dawley (90,70±4,37%). Disimpulkan bahwa persentase spermatozoa hidup pada tikus Wistar lebih tinggi daripada tikus Sprague-Dawley

Co-Authors . Amiruddin . Rusli Abdyad, Baradillah Afifuddin Afifuddin, Afifuddin Al Azhar Amiruddin A Arman Sayuti Aulanni’am a Azhar A, Azhar Basuki B. Purnomo budianto panjaitan Cut Dahlia Iskandar Cut Nila Thasmi, Cut Nila Darmawi Darmawi Darni, Dasrul Dasrul Dasrul Dian Masyitha Dini Melita, Dini Dwinna Aliza Erdiansyah Rahmi Erwin Erwin Etriwati, . Fahlevi, Zoerul Fajri, Fauzan Fakhrurrazi Fakhrurrazi Firda Muharrami, Firda Fitriani -, Fitriani Gholib G, Gholib Gholib Gholib, Gholib Ginta Riady Hafizuddin Hafizuddin, Hafizuddin Hamdan Hamdan Hamny H, Hamny Hamny Hamny Herrialfian, Herrialfian Huda, Raja Nurul I. Ketut Mudite Adnyane, I. Ketut Mudite Idawati Nasution Indra Saputra Simbolon, Indra Saputra Isa, M Ismail Ismail Joharsyah J, Joharsyah Juli Melia Kusuma, Afif Yuda Listin Handayani, Listin M Hasan M. Aris Widodo M. Nur Salim Mahdi Abrar Maryulia Dewi Muhammad Hambal Muhammad Hasan Muhammad Jalaluddin, Muhammad Muhammad Toras, Muhammad Muslim Akmal Mustafa Sabri Muttaqien Muttaqien, Muttaqien Nazaruddin Nazaruddin Nellita Meutia, Nellita Nofri Alfi, Nofri Nova Lianda, Nova Nurliana -, Nurliana Nuzul Asmilia Okta Hilda Kadar, Okta Hilda Ramadhana, Cut Erika Razali Daud Reza Sofa Hartuti, Reza Sofa Rinidar -, Rinidar Rinidar R, Rinidar Riski Ananda, Riski Rizky, Asri Roslizawaty -, Roslizawaty Roslizawaty r Rosmaidar Rosmaidar, Rosmaidar Rusli -, Rusli Rusli Rusli Setiadi, Rori Silvia Rizki, Silvia Siregar, Tongku Niswan Sri Wahyuni Srihadi Agungpriyono Sugito s Suriadi S, Suriadi Sutiman B. Sumitro Syafruddin s Syafruddin Syafruddin T. Armansyah Taufiq Purna Nugraha, Taufiq Purna Teuku Armansyah Teuku Reza Ferasyi Tongku Nizwan Siregar Triva Murtina Lubis Triva Murtina, Triva uropdana, simeon Yohanna Putri, Yohanna Yudha Bhaskara, Yudha Yudha Fahrimal Zahara, Widya Zahra, Amalia Fatma Zainuddin - Zainuddin Z Zuhrawati Zuhrawati, Zuhrawati Zuraidawati Zuraidawati, Zuraidawati