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PENGEMBANGAN BAHAN AJAR STATISTIKA BERBASIS MEDIA INTERAKTIF UNTUK SISWA SMK AKUNTANSI DI LAMPUNG BARAT Puspitasari, Heni; Koestoro, Budi; Caswita, Caswita
Jurnal Teknologi Informasi Komunikasi Pendidikan Vol 3, No 5 (2015): Jurnal Teknologi Informasi Komunikasi Pendidikan
Publisher : Jurnal Teknologi Informasi Komunikasi Pendidikan

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Abstract

The purposes of this study were, (1) to describe the conditions and the potential of the instructional materials development ; (2) to describe the process of the development of interactive media ; (3) to test the efficiency, the attractiveness and effectiveness of the use of media in learning. This research was using the method of development, with the subjects of accounting vocational students in West Lampung. Data were collected through observation, questionnaires, tests and analyzed descriptively and t-test examination. Conclusions of research were : (1) the conditionsand the potential for learning was not optimal, instructional material of interactive media was needed by students ; (2) this interactive media development process involued a computer expert, instructional design and material substance ; (3) the efficiency of product was 80,55; the attractiveness of product was 79,02% and the effectiveness of product was 3,60.Penelitian ini bertujuan, (1) mendeskripsikan kondisi dan potensi pengembangan bahan ajar ; (2) mendeskripsikan proses pengembangan media interaktif; (3) menguji efisiensi, kemenarikan dan efektivitas penggunaan media dalam pembelajaran. Penelitian dengan metode pengembangan,  dengan subyek siswa SMK Akuntansi di Lampung Barat. Data dikumpulkan melalui observasi, angket,tes dan dianalisis secara deskriptif dan uji t-test. Kesimpulan penelitian adalah: (1) kondisi dan potensi pembelajaran belum optimal, bahan ajar yang dibutuhkan siswa adalah media interaktif ; (2) proses pengembangan media interaktif ini divalidasi ahli komputer, desain pembelajaran dan substansi materi ; (3) efisiensi produk sebesar 80,55 ; kemenarikan produk sebesar 79,02% dan efektivitas produk sebesar 3,60.Kata kunci : bahan  ajar, media interaktif, statistika
Changes of T CD4+ and CD8+ Lymphocyte Level Before and After Treatment in Childhood Tuberculosis Puspitasari, Heni; Nataprawira, Heda Melinda; Hilmanto, Dany
JOURNAL OF THE INDONESIAN MEDICAL ASSOCIATION Majalah Kedokteran Indonesia Vol 63, No 9 (2013): Journal of the Indonesian Medical Association Majalah Kedokteran Indonesia
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA

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Abstract

Introduction: Cellular immunity plays a central role in defenses against intracellular bacteria Mycobacterium tuberculosis. Antituberculosis treatment could be expected to increase T CD4+ and CD8+lymphocytes as dominant protective lymphocytes. However, the changes of lymphocyte profile blood in childhood tuberculosis (TB) still unclear. This study aimed to evaluate changes of CD4+ and CD8+T lymphocytes level before and after 2 months antituberculosis in childhood TB.
THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 3 (2016)
Publisher : Institute of Topical Disease

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Abstract

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun’s leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun’s leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells. 
PRODUCTION AND CHARACTERIZATION OF IMMUNOGLOBULINE YOLK AS ANTI ANTIGEN MEMBRANE TOXOPLASMA GONDII Puspitasari, Heni; Praptiwi, Yuliana; Suwanti, Lucia
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 2 (2016)
Publisher : Institute of Topical Disease

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Abstract

Toxoplasma gondii is an obligate parasite intracellular which can infected human and other mammalian. Immunoglobulin Y technology  offers several advantages better than antibody production in mammals. This research was aimed to get immunoglobulin Y from egg yolk, to prove that antibody against membrane T. gondii antigen can produced from immunoglobulin Y and to know the characterization of  immunoglobuline Y according to  molecular weight by SDS PAGE and reactivation of  antibody antigen  by Western Blott. This research devided  from many step : passase tachyzoites T. gondii into mice by peritoneal infection, cultivated  the tachyzoite from intraperitoneal fluid, preparation of  membrane antigen tachyzoite T. gondii, then  immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blott.  The result of this resarch showed that immunoglobulin Y from egg yolk can  produced antibody against protein membrane T. gondii. The result of analyzed profile protein immunoglobuline  Y according SDS PAGE  has molecular weight 179,8 kDa. Analyzed from Western Blott showed that immunoglobulin Y can recognize antigen epitope of  T. gondii on molecular weight 35,7 kDa and 78,8 kDa.