Rani Agustina Wulandari, Rani Agustina
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Pengendalian Mutu Benih Tebu (Saccharum officinarum L.) Mata Tunas Tunggal Menggunakan Pengaturan Kadar Lengas Media Serbuk Abu Sekam Padi Panuntun, Henricus Tegar; Taryono, Taryono; Wulandari, Rani Agustina
Vegetalika Vol 6, No 1 (2017)
Publisher : Vegetalika

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Abstract

Bibit tebu mata tunas tunggal memiliki umur simpan yang relatif pendek. Apabila tanpa perlakuan, daya kecambah mata tunas tunggal sudah menurun pada umur simpan 2 hari. Oleh karena itu, dibutuhkan metode penyimpanan yang tepat untuk memperpanjang umur simpan, sehingga kualitasnya masih baik saat akan ditanam di lahan petani. Penelitian ini bertujuan untuk mengetahui kadar lengas media simpan dan umur simpan tebu terbaik untuk mempertahankan mutu bibit tebu mata tunas tunggal. Penelitian ini dilakukan dengan menggunakan rancangan perlakuan faktorial 3 × 4 + 1 dalam rancangan lingkungan acak lengkap (RAL). Faktor pertama adalah media simpan yang terdiri dari media simpan serbuk abu sekam padi dengan penambahan kadar lengas media 0, 20, 40 dan 60 %. Faktor kedua adalah klon tebu yang terdiri dari klon Kidang Kencana, klon Bululawang, dan klon VMC. Pengamatan dilakukan pada umur simpan 0, 4, 8, 12 hari. Hasil penelitian menunjukkan bahwa terdapat interaksi antara media simpan dan klon yang digunakan pada  jumlah mata tunas mati, jumlah mata tunas tumbuh, indeks vigor (IV), tinggi tanaman, jumlah daun, diameter batang sedangkan pada pengamatan lainnya, media simpan dan klon tebu tidak memberikan pengaruh yang nyata.  Penyimpanan menggunakan media dengan kadar lengas 20% secara umum sedikit menurunkan kualitas benih. Klon VMC menunjukkan perkecambahan benih paling baik dilihat dari gaya berkecambah dan indeks vigornya. Dari pengamatan mutu benih diketahui penambahan kadar lengas mampu memperpanjang masa simpan hingga umur simpan 12 hari , sedangkan pada mutu bibit penambahan kadar lengas mampu memperpanjang masa simpan hingga 8 hari.
Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D Dharmayanti, Krisna; Sulistyaningsih, Endang; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 2, No 3 (2017): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.26276

Abstract

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant  and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0?0, 2?1, 2?2, 2?3, and 2?4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1? 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.
Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids Murianingrum, Mala; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 1 (2018): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.905 KB) | DOI: 10.22146/ipas.34673

Abstract

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.
Pendugaan Parameter Genetik Komponen Hasil untuk Seleksi Tidak Langsung Tanaman Padi (Oryza sativa L.) Berdaya Hasil Tinggi Faiqon, M. Mujadid; Supriyanta, Supriyanta; Wulandari, Rani Agustina
Vegetalika Vol 6, No 2 (2017)
Publisher : Vegetalika

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Abstract

Seleksi langsung terhadap daya hasil seringkali sulit dilakukan karena sifat alami hasil yang kompleks dan besarnya pengaruh lingkungan terhadap hasil. Seleksi tidak langsung mendasarkan pada komponen hasil yang berkorelasi terhadap hasil dapat dilakukan sebagai solusi untuk masalah tersebut. Pendugaan parameter genetik dilakukan untuk memperoleh informasi mengenai komponen hasil yang dapat digunakan untuk meningkatkan hasil per hektar. Sembilan genotipe padi  diasumsikan  berdistribusi  random  ditanam  mengikuti  rancangan RCBD di dua lokasi, Klaten dan Sleman. Komponen varian dan kovarian yang diduga melalui ANOVA dan ANCOVA digunakan untuk menduga heritabilitas, korelasi genetik, koheritabilitas, dan rasio nilai harapan CRA/RA. Analisis lebih lanjut untuk korelasi genetik dilakukan dengan analisis lintas untuk memperoleh informasi tambahan  mengenai hubungan  antara hasil dengan komponen hasil. Tinggi tanaman, panjang daun bendera, umur berbunga, umur panen, bobot 100 butir biji, jumlah malai per rumpun, panjang malai, dan kerapatan malai diketahui memiliki heritabilitas tinggi (lebih dari 0,7) dan lebih tinggi dibandingkan heritabilitas hasil per hektar (0,55 di Klaten dan 0,42 di Sleman). Jumlah malai per rumpun memiliki korelasi genetik positif dan tinggi dengan hasil per hektar sementara panjang daun bendera dan umur berbunga memiliki pengaruh langsung positif dan tinggi terhadap hasil per hektar di lokasi Klaten. Bobot 100 butir biji dan panjang malai memiliki korelasi genetik yang positif dan tinggi terhadap hasil per hektar sementara tinggi tanaman dan jumlah biji per malai memiliki pengaruh langsung yang tinggi dan positif. Akan tetapi, tidak terdapat komponen hasil yang memiliki rasio nilai harapan CRA/RA lebih tinggi dari 1 yang menunjukkan bahwa tidak ada komponen hasil yang dapat meningkatkan kemajuan genetik hasil per hektar melalui seleksi tidak langsung. Seleksi langsung terhadap daya hasil lebih efektif dibandingkan dengan seleksi tidak langsung melalui komponen hasil tertentu.
Pengaruh Pemeraman Eksplan Daun dengan Kolkisina Secara In Vitro terhadap Keberhasilan Pembentukan Terung Tetraploid Nabihaty, Fathin; Taryono, Taryono; Wulandari, Rani Agustina
Vegetalika Vol 7, No 1 (2018)
Publisher : Vegetalika

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Abstract

Perbaikan tanaman untuk meningkatkan daya tawar terung dapat dilakukan melalui poliploidisasi. Pemberian kolkisina secara in vitro berpeluang meningkatkan efisiensi poliploidisasi. Penelitian dilakukan untuk mengkaji pengaruh pemeraman eksplan daun dengan kolkisina secara in vitro terhadap keberhasilan poliploidisasi dan pembentukan terung tetraploid (4x), serta mendapatkan tetraploid berdasarkan ciri morfologi planlet dan jumlah kromosom. Tiga aras periode peram dengan 2,5 mM kolkisina (24, 48, dan 72 jam) dan satu perlakuan pemeraman tanpa kolkisina sebagai pembanding, diujicobakan pada lima jenis terung (Rimbang, Lokal Bantul, Pipit, Hijau Malang, dan Limao) menggunakan rancangan acak lengkap. Tahap utama yang dilalui eksplan yaitu pra-pemeraman, pemeraman dengan kolkisina, regenerasi, penggantian media tanam 1-6 kali, pengakaran, dan regenerasi ulang hingga 2 kali. Tata cara ini berhasil membentuk Terung Rimbang poliploid yang terdiri dari 2x+3x dan 3x+4x. Jumlah individu poliploid diperkirakan sebanyak 59%, sisanya 41% merupakan diploid. Planlet yang memiliki sel tetraploid sekaligus ploidinya tertinggi diperoleh dari pemeraman kolkisina selama 72 jam.
Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D Dharmayanti, Krisna; Sulistyaningsih, Endang; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 2, No 3 (2017): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.26276

Abstract

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant  and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0–0, 2–1, 2–2, 2–3, and 2–4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1– 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.
Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids Murianingrum, Mala; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 1 (2018): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.34673

Abstract

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.
Yield Components of Some Sesame Mutant Populations Induced by Gamma Irradiation Aristya, Vina Eka; Taryono, Taryono; Wulandari, Rani Agustina
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 10, No 2 (2018): Oktober 2018
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/btsm.v10n2.2018.64-71

Abstract

Sesame is an producing seed whose oil is commercially needed. Breeding attempts to improve the productivity of sesame and yield components are induction of gamma ray irradiation mutations (Co-60). This study was aimed to identify effects of induced mutation by gamma rays irradiation in quantitative characteristics and yield of sesame in M4 generation originated from local cultivars. Two types of sesame (black and white) are irradiated with eight doses (100-800 Gy) of Co-60. The result showed a high variation in almost all morphological characters and modified the character of stem height from base to first branch, number of capsules per plant, biomass yield per plant, and seed yield per plant. Sesame irradiated with 600 Gy Co-60 doses has a beneficial effect on the number of capsules (black:120.23; white: 255.23, respectively) and the weight of 1000 seeds (black:3.63 g; white: 4.55 g, respectively). Genotypic Coefficient of Variation in M4 generation were recorded for high value for characters number of primary branches (30.16%), stem height from base to the first branch (30.96%), stem height from base to first capsule (14.82%), number of secondary branches (53.64%), number of nodes to first flower (72.66%), number of capsules/plant (44.90%), biomass yield/plant (28.37%), and seed yield/plant (36.68%). Genetic variability of plant population is very important for plant breeding program and to sustain level of high productivity.Komponen Hasil Beberapa Populasi Mutan Wijen yang Diinduksi oleh Iradiasi GammaWijen adalah tanaman penghasil biji yang minyaknya dibutuhkan secara komersial. Upaya pemuliaan untuk meningkatkan produktivitas wijen dan komponen hasil adalah dengan induksi mutasi iradiasi sinar gamma (Co-60). Penelitian ini bertujuan mengidentifikasi pengaruh induksi mutasi iradiasi sinar gamma pada karakter kuantitatif dan hasil pada generasi M4 wijen yang berasal dari kultivar lokal. Dua jenis wijen (hitam dan putih) diiradiasi dengan delapan dosis (100-800 Gy) Co-60. Sejumlah pengaruh mutasi wijen berhasil menunjukkan variasi yang tinggi pada hampir semua ciri morfologi dan memodifikasi karakter tinggi batang dari pangkal ke cabang pertama, jumlah kapsul/tanaman, hasil biomassa/tanaman dan hasil biji/tanaman. Wijen yang diiradiasi dengan dosis 600 Gy Co-60 memiliki efek menguntungkan pada jumlah kapsul (hitam:120,23; putih: 255,23) dan berat karakter 1000 biji (hitam:3,63 g; putih: 4,55 g). Koefisien Keragaman Genotipik pada generasi M4 dicatat nilai tertinggi pada karakter jumlah cabang primer (30,16%), tinggi batang dari pangkal ke cabang pertama (30,96%), tinggi batang ke kapsul pertama (14,82%), jumlah cabang sekunder (53,64%), jumlah ruas ke bunga pertama (72,66%), jumlah kapsul/tanaman (44,90%), hasil biomassa/tanaman (28,37%), dan hasil biji/tanaman (36,68%). Keragaman genetik dari populasi tanaman sangat penting untuk program pemuliaan tanaman dan mempertahankan produktivitas yang tinggi.
Genetic Variation in Selected Individuals Based on Number of Capsule in M5 Sesame Mutan Lines Detected by RAPD Kholida, Masruru; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.32699

Abstract

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 ? 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).  
Genetic Variation in Selected Individuals Based on Number of Capsule in M5 Sesame Mutan Lines Detected by RAPD Kholida, Masruru; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.32699

Abstract

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 – 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).